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Development of multiplex PCR for detection of vancomycin resistant enterococci(VRE) and epidemiological application in Korea

  • Seo, Keun-seok;Song, Deok-jln;Gwyther, M.M.;Park, Yong-ho
    • Korean Journal of Veterinary Research
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    • v.39 no.2
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    • pp.343-352
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    • 1999
  • Vancomycin resistant enterococci (VRE) have emerged as an important nosocomial pathogen. Since 1989 the Center for Disease Control, United States, has reported a rapid increase in the incidence of enterococcal bacteremia and endocarditis infection by VRE. It was suggested that the use of avoparcin was associated with the appearance of VRE in animal husbandry. To date, several detection methods have been used based on conventional methods of culture and gene detection. However, these methods have some limitations such as time-consuming, laborious and additional differential needs. Therefore, In this study a multiplex PCR method was established to detect and differentiate resistance types of enterococci which specifically amplify the four van genes encoding vancomycin resistance elements. Using the method, we investigated the incidence rates and types of VRE from farms using or not using avoparcin. A total of 1091 animal fecal samples were collected from 70 pig and 32 poultry farms. A total of 425 of enterococci were isolated from samples. Of the 425 isolates, 11 of the them showed a pattern of high-level vancomycin resistance (MIC : $64{\sim}256{\mu}g/ml$) which was associated with the presence of the vanA or vanB gene. Fifty-seven isolates showed a pattern of low-level vancomycin resistance (MIC : $3{\sim}8{\mu}g/ml$) associated with the vanC-1 or vanC-2 gene. Interestingly, all isolates with high-level vancomycin resistance were from farms that have never used avoparcin. Moreover, the high-level VRE isolation rate in Korea (2.58%) was much lower than that of other countries (50% in England, 7% in Belgium) where avoparcin have been used. In conclusion, the multiplex PCR method established in this study could be applied for detection of VRE.

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A Study on the Exquisite Revolution of the 16th Century Ruff Collar and Ruff Cuffs in the Portraitures of Queen Elizabeth I (엘리자베스 1세의 초상화에 나타난 16세기 러프 칼라와 러프 커프스의 미시적 변화 연구)

  • Bae, Soo-Jeong
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.2
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    • pp.167-180
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    • 2011
  • The portraits created in the age of Queen Elizabeth I show a very exquisite description about the ruff collar and cuffs representing the notable evolution of periodical costumes from a detailed viewpoint. This thesis analyzes the ruff collar and cuffs of the portraits of Queen Elizabeth I. The method used selects 32 pieces from the portraits of Queen Elizabeth I that are considered excellent depictions of a ruff collar and cuffs, to investigate size, form and decorations. Conclusively, the ruff collar tends to be extremely enlarged and thickened (coinciding with the development of glue) while the cuffs denote the aspect of double cuffs coupled with the turn-back cuffs without a change in size. These traits were widely shown from 1585 to 1587, while after 1588 the ruff cuffs completely disappeared in the portraits of Elizabeth I. At the same time, the change of a ruff collar was remarkable, the collar being in the erect position behind the head (with the use of supporters), of a consistent thickness and decoration of elaborate lace. During the transition period from the $16^{th}$ century to the $17^{th}$ century the earliest change was in cuffs followed by collars. This research is a helpful guide to detect the relatively exact date of portraits not definitively identified in the latter part of the $16^{th}$ century and to observe the microscopic evolution of costumes in the latter part of the $16^{th}$ century.

Disaster Vulnerability Analysis for Steep Slope Failure (급경사지 재해도 분석)

  • Choi, Eun-Kyeong;Kim, Sung-Wook;Kim, Sang-Hyun;Park, Dug-Keun;Oh, Jeong-Rim
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.03a
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    • pp.930-939
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    • 2009
  • Most of steep slope failures occurring in Korea have appeared during the localized heavy rain period, whereas the evaluation model of a disaster vulnerability analysis that has been proposed to date, has been prepared in consideration only of external factors comprising geographical features. This study calculated a wetness index and a contributory area which delivers moisture to the upper slant surface during the rainfall period, and also conducted a disaster vulnerability analysis in consideration of the convergence of surface water as well as the water system created during the occurrence of rainfall by including a curvature that shows a close relevance with the shape of the minute water system that is created temporarily during the occurrence of rainfall and with the convergence and divergence of surface water. When compared with a steep slope failure occurring within a selected model district in order to verify the prepared disaster analysis, a landslide occurring in the model district had emerged in a region in which the disaster vulnerability analysis was high and the density of the minor water system was also high. If these research results are extended nationwide, it is the most effective to use a disaster vulnerability analysis and the density of the minute water system; and it is supposed to be the simplest and the most effective method for preparing a disaster analysis of mountainous land shape such as the model district.

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Considerations of Automatic Passenger Counting System using Infrared Sensors at doorway in Overseas Railway Transit (적외선 센서를 이용한 자동 승객 계수 시스템에 대한 고찰)

  • Kim, Jin-Seok;Gwak, Ho-Seung
    • Proceedings of the KSR Conference
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    • 2009.05a
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    • pp.418-423
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    • 2009
  • Unlike domestic railway transportation system in which majority of station are equipped with gate access controller and ticket office, it has been a very common practice in overseas railway transit or railway station that they use a pressure door mat, infrared-sensors or CCTV cameras so as to automatically determine the number of passenger onboard and alight and to reflect the information to their business (i.e., deployment of vehicles and human resources). The data collected by the automatic passenger counting (APC) system provides methods how to obtain the information about the number of passenger using the vehicles on the basis of date, time and stop(station) which enables large-scaled transit company to create profits through effective vehicle deployment and management of their employees. This paper addresses the basic features of the automatic passenger counting system using infrared sensor and describes those of the extended APC system in conjunction with wireless technologies such as GPS, WLAN or Cellular network.

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The Study of the Effects of Factors on the Attitude and the Using Intention of Mobile Fashion Shopping - Focused on 20's${\sim}$30's Female - (모바일 패션 쇼핑에 대한 태도 및 이용 의도에 영향을 미치는 요인에 관한 연구 - 20${\sim}$30대 여성을 중심으로 -)

  • Kim, Min-Jung
    • The Research Journal of the Costume Culture
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    • v.17 no.4
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    • pp.709-722
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    • 2009
  • At the end of April, 2009, the number of mobile phone users reached about 46 million in Korea. Consequently, the mobile shopping market is growing rapidly, and fashion products are one of the most concerned categories in mobile shopping market. In the meantime, the growth rates of domestic fashion industry and fashion retail market are forecasted very slow. In this situation, however, the growth rate of on-line shopping market including internet shopping, TV home shopping, and mobile shopping is estimated more than 12% per year until 2010. Particularly, mobile shopping area is forecasted the most rapid growing field. The objective of this study were to confirm the factors which had influences on mobile fashion shopping and to examine the effects of these factors affect the attitude toward mobile fashion shopping and the using intention of mobile fashion shopping. With the results, marketing strategies for mobile fashion shopping companies would be proposed. Date were gathered from 125 respondents of 20's and 30's females and were analyzed by AMOS 7.0. The key points of the results are; 1) The 5 factors of instant access, individualization, amusement, usefulness and ease of use which extracted from preceding researches were confirmed as the characteristics of mobile fashion shopping, 2) among these factors, amusement and usefulness had positive influences on the attitude toward mobile fashion shopping, 3) reliability on mobile fashion shopping didn't had an influence on the attitude toward mobile fashion shopping significantly, but had a positive influence on the using intention of mobile fashion shopping directly, and 4) the attitude toward mobile fashion shopping affects using intention of mobile fashion shopping positively. With these results, mobile fashion shopping companies can establish marketing strategies from various angles.

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Design and Development of Scenario-Based Simulation System to Improve Shipbuilding Execution Scheduling Assessment -A Case Study on Panel Line- (시나리오 기반 조선 실행계획 평가 향상을 위한 시뮬레이션 시스템 개발 -패널라인 개발 사례를 중심으로-)

  • Back, Myunggi;Kim, Youngmin;Hwang, Inhyuck;Lee, Kwang-Kook;Ryu, Cheolho;Shin, Jong Gye
    • Korean Journal of Computational Design and Engineering
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    • v.18 no.3
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    • pp.211-223
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    • 2013
  • Today's ever-increasingly competitive shipbuilding market makes it essential for a shipbuilding company to have more efficient production processes and higher productivity as well as better design ability to obtain its competitiveness. A well-established production execution schedule plays an indispensable role to achieve this goal. Most shipbuilding companies carry out an evaluation on their mid-term plan once it is established. However, no evaluation activity exists for a production execution schedule, because practically all the companies depend on the field workers for the production execution scheduling. In this study, a prototype of a ship production execution schedule evaluation system is developed based on the component based design (CBD) methodology. This system enables one to make a production execution schedule that reflects up-to-date shipyard situation and to validate whether the schedule is feasible or not by running a production simulation according to the schedule. Users can also make use of the system as a decision supporting tool that compares several different execution schedules and evaluates which one is the best execution schedule.

Evaluation of Residual Tensile Load of Field Ground Anchors Based on Long-Term Measurement (현장 그라운드 앵커 장기거동 분석을 통한 잔존긴장력 평가)

  • Park, Seong-yeol;Lee, Sangrae;Jung, Jonghong;Cho, Wanjei
    • Journal of the Korean Geotechnical Society
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    • v.36 no.8
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    • pp.35-47
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    • 2020
  • For permanent anchors used for slope reinforcement, bearing capacity and durability should be secured during the period of use. However, according to recent domestic and foreign studies, phenomena such as tension fractures, damage to anchorages, deformation and damage to slope and reduction of residual load over time have been reported along the long-term behavior of the anchors. These problems are expected to increase in the future, which will inevitably lead to problems such as increasing maintenance costs and relevant facility collapse. It is necessary to improve maintenance procedures and methods of ground anchors more practically. In this study, the problems and limitations of domestic maintenance methods were analyzed by conducting a literature study, and the measurement data of load cells installed on the install ground anchors were analyzed to determine the change in the residual load with regard to the elapsed date of the anchors. Based on the results, the effect of the construction conditions of anchors and the soil compositions on the increase and decrease of load were identified.

The Prediction of Health care Outcome of Total Hip Replacement Arthroplasty Patients using Charlson Comorbidity Index (Charlson Comorbidity Index를 활용한 고관절치환술 환자의 건강결과 예측)

  • Choi, Won-Ho;Yoon, Seok-Jun;Ahn, Hyeong-Sik;Kyung, Min-Ho;Kim, Kyung-Hun;Kim, Kyeong-Uoon
    • Korea Journal of Hospital Management
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    • v.14 no.1
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    • pp.23-35
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    • 2009
  • The objectives of the present study is to examine the validity of Charlson Comorbidity Index(CCI) based on medical record data; to utilize the index to determine outcome indexes such as mortality, length of stay and cost for the domestic patients whose have received total hip arthroplasty. Based on medical record date, 1-year Mortality was analyzed to be 0.664 of C statistic. The $R^2$ for the predictability for length of stay and the cost was about 0.0181, 0.1842. Fee of national health insurance and total cost including the cost not covered by insurance, also had statistically significance above 3 points of Charlson point score(p=0.0290, 0.0472; $p.{\le}0.05$). The 1-year mortality index, length of stay and cost of the total hip arthroplasty patients which was obtained utilizing CCI have a limitative prediction power and therefore should be carefully analyzed for use.

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A Study on the Actual Condition of Using Low-priced Cosmetics and on the Purchasing Behavior in Female Undergraduates (여대생들의 저가 화장품에 대한 사용실태 및 구매행동에 관한 연구)

  • Kim, Ju-Duck
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.2
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    • pp.177-189
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    • 2011
  • Women starts to make up after high school graduation. They have low economic power. But they select cosmetics by themselves and have high demand. Therefore, they can be major customers of low-priced cosmetics and it can be regarded as independent cosmetics market. Thus, this study analyzes the purchasing propensity of lowpriced cosmetics in female undergraduates, who are main targets of low-priced cosmetics, satisfies the more customers desire through further segmenting the targets, suggests a continuous developmental plan for low-priced cosmetics through securing potential customers and segmenting market, and analyzes and typologizes female undergraduates lifestyle. The aim is to grasp the actual condition of using the low-priced cosmetics and the satisfaction with purchase, and to utilize it as basic date of inducing right consumption culture of female undergraduates. For this study, 305 reliable questionnaires are analyzed from the total 320 questionnaires and spss win 15.0 program was used. The results were as follows. About 90.1 percent of female undergraduates had experience using low-priced cosmetics and standard of priority selection was the quality of low-priced cosmetics. The main reason not to use was low price image.

Plant Height, Dry Matter Yield and Forge Quality at Different Maturity of Whole Crop Rice (수확시기가 사료용 벼의 초장, 건물수량 및 사료성분에 미치는 영향)

  • 성경일;홍석만;김병완
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.1
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    • pp.53-60
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    • 2004
  • Recently, there has been a growing tendency to use whole crop rice for feeds as there is an increased rice supply and a decrease in the demand for rice in Korea. Little information, however, is available about the management and utilization of whole crop rice for feeds. This study was conducted to decide the optimum harvest date through examining the plant height, dry matter(DM) yield and feed composition of whole crop rice harvested at different maturity. Field study was established early in May until October 7th on a rice field at Yupori, Sinbuk-yeup, Chunchon, Kangwon-Do. "Ilpum" mutant rice was sown at 52 kg/ha, and N, P and K were applied at 209, 113 and 81 kg/ha, respectively. The rice was harvested at six different mature stages for analysis; booting stage; 17 Aug., milk-ripe stage; 27 Aug., dough stage; 7 Sep., yellow ripe stage; 17 Sep., dead ripe stage; 27 Sep. and full ripe stage; 7 Oct. Plant height was 77 cm in booting stage (P<0.05) which was lower when compared to the other stages among which the heights did not differ with average range of 93∼97 cm. The highest dry matter yield was observed in yellow ripe stage (22.8 T/ha), which was followed by dead ripe stage (19.3 T/ha), full ripe stage (19.3 T/ha), and dough stage (15.1 T/ha). Crude protein (CP) and Neutral detergent fiber (NDF) concentrations consistently decreased with maturity. The highest concentrations of CP and NDF were obtained in booting stage with 9 and 73.6%, respectively (P<0.05), and lowest in full ripe stage with 5.8 and 64.6%, respectively (P<0.05). The concentration of total digestible nutrient (TDN) was highest in booting stage (59.7%); however no significant difference was found among other stages. The concentrations of Ca and P were not different among mature stages. According to these results, the yellow ripe period is appropriate to harvest the whole crop rice for forage considering dry matter yields, feed compositions and TDN concentrations.