• BMB Reports
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• 제44권1호
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• pp.28-33
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• 2011

MicroRNAs are potential key regulators in mesenchymal stem cells chondrogenic differentiation. However, there were few reports about the accurate effects of miRNAs on chondrogenic differentiation. To investigate the mechanisms of miRNAs-mediated regulation during the process, we performed miRNAs microarray in MSCs at four different stages of TGF-${\beta}3$-induced chondrogenic differentiation. We observed that eight miRNAs were significantly up-regulated and five miRNAs were downregulated. Interestingly, we found two miRNAs clusters, miR-143/145 and miR-132/212, kept on down-regulation in the process. Using bioinformatics approaches, we analyzed the target genes of these differentially expressed miRNAs and found a series of them correlated with the process of chondrogenesis. Furthermore, the qPCR results showed that the up-regulated (or down-regulated) expression of miRNAs were inversely associated with the expression of predicted target genes. Our results first revealed the expression profiles of miRNAs in chondrogenic differentiation of MSCs and provided a new insight on complicated regulation mechanisms of chondrogenesis.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.176-176
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• 2003

Methylmercury is known to have devastating effects on the mammalian nervous system. When human neuroblastoma SH-SY5Y cells were treated with methylmercury at sublethal concentrations (6.25 uM), up-regulated genes (39) ＆ down-regulated genes (19) were identified by human 8k cDNA microarray. These genes are related with microtubule process, signal transduction pathway and cell death (apoptosis), Apoptosis-associated genes, HSP70, CDK inhibitor 1, FOS-like antigen were up-regulated and microtubule related genes like villin and dynein down-regultaed. To confirm the presence of apoptosis in cultured SH-SY5Y cells treated 6.25 and 1 uM methylmercury, we applied Annexin V-FITC assay followed by flow cytometric measurements after 6 and 24h. Studies on transcriptional and molecular effect by methylmercury may provide an insight into the neurotoxic effects of methylmercury in human neuronal cells and a possibility to develop more efficient and exact monitoring system of heavy metals as ubiquitous environmental pollutants.

• Genomics & Informatics
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• 제16권4호
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• pp.25.1-25.5
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• 2018

Coronary artery disease (CAD) is one of the leading causes of death and disability all around the world. Recent studies have revealed that aberrantly regulated long non-coding RNA (lncRNA) as one of the main classes of cellular transcript plays a key regulatory role in transcriptional and epigenetic pathways. Recent reports have demonstrated that circulating lncRNAs in the blood can be potential biomarkers for CAD. HOTAIR is one of the most cited lncRNAs with a critical role in the initiation and progression of the gene expression regulation. Recent research on the role of the HOTAIR in cardiovascular disease lays the basis for the development of new studies considering this lncRNA as a potential biomarker and therapeutic target in CAD. In this study, we aimed to compare the expression of HOTAIR lncRNA in the blood samples of patients with CAD and control samples. The expression level was examined by semi-quantitative reverse transcriptase polymerase chain reaction technique. Our data shows that expression of HOTAIR is up-regulated in blood samples of patients with CAD.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.129-129
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• 2003

Methylmercury is known to have devastating effects on the mammalian nervous system. When human neuroblastoma SH-SY5Y cells were treated with MeHg at sublethal concentrations (6.25 uM), up-regulated genes (39) ＆ Down-regulated genes (19) were identified by microarray.(omitted)

• 대한화학회지
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• 제35권5호
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• pp.560-568
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• 1991

국내에서 현재 규제되고 있는 농약에 대해 신속, 간단한 동시분석법이 연구되었다. 시료는 아세톤과 메탄올로 추출하고 물을 가한 다음, $CH_2Cl_2$로 분배시킨다. 추출물은 Bio-Beads S-X3 컬럼에서 cyclohexane-methylene chloride의 용출액 11ml를 받아 불순물을 분리, 제거하고 ECD/NPD가 병렬되게 BP-1(methyl silicone) 모세관 컬럼에 연결된 기체 크로마토그래피로 분석한다. 농약 첨가에 의한 평균 회수율은 쌀에서 83% 이상이었고, 콩에서 3종 농약을 제외하면 81% 이상이었다. 검출한계는 쌀에서 0.002 ppm(${\alpha}$-BHC 등)~0.3 ppm(carbaryl 등) 이었다.

• 약학회지
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• 제52권2호
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• pp.111-116
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• 2008

Diethylstilbestrol (DESB) is a synthetic estrogen not only that routinely prescribed, but also that known to be a teratogen. In this study, we found a novel pharmacological feature that DESB is able to positively modulate CD29 $({\beta}1-integrin)$ function. Thus, DESB up-regulated homotypic cell-cell adhesion of monocytic U937 cells mediated by CD29. However, DESB did not increase the surface level of CD29 and its binding activity to ligand (fibronectin), according to flow cytometric analysis and cell-fibronectin adhesion assay. Instead, the DESB-mediated up-regulation of cell-cell adhesion was blocked by several signaling enzyme inhibitors. Treatment of U0126 [an extracellular signal-regulated kinase (ERK) inhibitor], SB20358 (a p38 inhibitor) or Rp-8-pCPT-cGMP (a protein kinase G inhibitor) clearly inhibited DESB-mediated up-regulation of cell-cell adhesion induced by CD29. However, estrogen receptor antagonist ICI 182,780 failed to abrogate DESB effect. Therefore, our data suggest that DESB may up-regulate CD29-mediated cell-cell adhesion via modulating intracellular signaling enzymes such as ERK, PKG, and p38, independent of estrogen receptor function.

• 한국문헌정보학회지
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• 제47권4호
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• pp.103-124
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• 2013

본 연구는 문헌정보학과 교과목에 문제기반학습을 적용한 자기조절학습모형을 설계해 실행한 후 학습자의 인지적 동기적 자기조절학습능력의 변화를 살펴보는 것을 목적으로 하는 연구이다. 이를 위해 본 연구는 수강생들의 자기조절학습능력의 변화를 사전 사후 동일 검사지로 설문조사하여 살펴보았다. 인지적 동기적 자기조절학습능력은 각각 일반적 영향과 상관관계를 살펴보고 해당 자기조절학습능력의 세부하위요인들의 학습 전 후 변화를 측정하였으며 통계적 유의성은 대응표본 t-test로 분석하였다. 연구결과 문제기반학습법은 학생들의 인지적 동기적 자기조절학습능력의 향상에 효과가 있음을 밝혀냈으며 동기적 자기조절학습능력에 있어서는 후속 연구에 대한 가능성을 발견하였다.

• BMB Reports
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• 제35권6호
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• pp.623-628
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• 2002

The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential display-polymerase chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counter-regulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3'UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12 h, and lasted until 24 h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

• 예술인문사회 융합 멀티미디어 논문지
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• 제9권3호
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• pp.51-59
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• 2019

외국어 학습이론에서 자기조절학습, 교과흥미, 학습몰입, 자기효능감은 주요 구성개념들로 연구되었다. 이에 본 연구에서는 이 구성개념 간 상호 미치는 영향관계를 파악하기 위하여 상기 구성개념들을 토대로 연구모형을 설정하고 SEM분석을 통하여 그 내용을 파악하고자 하였다. 경남에 위치한 중학교 학생들을 대상으로 수집한 자료를 분석한 결과는 첫째, 교과흥미는 학습몰입에 긍정적 영향을 미치는 것으로 나타났다. 둘째, 교과흥미는 자기효능감에 긍정적 영향을 미치고 또한 자기조절학습에 긍정적 영향을 미쳤으며, 셋째, 학습몰입은 자기효능감에 긍정적 영향을 미치는 것으로 나타났고 넷째, 자기조절학습은 자기효능감에 긍정적 영향을 미쳤고 다섯째, 자기조절학습은 학습몰입에 긍정적 영향을 미쳤다. 상기 분석결과를 살펴보면 영어교육의 경우 학생들의 교과흥미는 학습몰입을 가져오고 자기효능감을 증진시키며 자기조절학습 능력을 증진하여 결국은 성과로 이어지게 된다.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.177-190
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• 2006

Objectives : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cell lines. Oligonucleotide microarray and proteomic approaches were employed to screen the differential expression genes. Methods : GRR~HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS (0.1, 0.5, 1.5, 10, $20mg/m{\ell}$) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with $1.5mg/m{\ell}$ of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : It has no cytotoxic effects on both HepG2 cells in all concentrations(0.1, 0.5, 1.5, 10,$20mg/m{\ell}$). In oligonucleotide microarray assay, the number of more than twofold differentially regulated known genes was 320 with 6 up-regulated and 314 down-regulated genes in HepG2 cells. In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. One down -regulated protein was protein disulfide isomerase and up-regulated proteins were fatty acid binding protein 1 and 14-3-3 gan1lTIa protein by $1.5mg/m{\ell}$ of CRR-HAS. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.