• Korean Journal of Microbiology
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• v.40 no.2
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• pp.65-72
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• 2004

Instability of some eukaryotic sequence propagated in prokaryotic hosts is a frequently observed phenomenon. It is well documented that long inverted repeats, AT-rich sequences with structures like Z-DNA are extremely unstable in E. coli. These sequences may either be under-represented or even lost when cloned in E. coli. When we analyzed the polymorphic pattern for several tandom repeat (TR) in human SCKI gene, we found some TR regions were frequently deleted from plasmids and had difficult problem for their sequencing. These regions may result in non-clonability of the DNA sequence. Here we have cloned two difficult TR regions under low temperature and made two library for DNA sequencing using a nebulizer or sonicator. This study will help to determine the unstable genomic elements in complex mammalian genome.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.11
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• pp.1434-1443
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• 1999

The turbulent fluctuations of temperature and two components of velocity have been measured with hot- and cold-wires in the Thermally Stratified Wind Tunnel(TSWT). Using the fin-tube heat exchanger type heaters and the neural network control algorithm, both stable ($dT/dz=109.4^{\circ}C$) and unstable ($dT/dz=-49.1^{\circ}C$) stratifications were realized. An ambient air jet was issued normally into the cross flow($U_{\infty}=1.0 m/s$) from a round nozzle(d = 6 mm) flushed at the bottom waII of the wind tunnel with the velocity ratio of $5.8(U_{jet}/U_{\infty})$. The characteristics of turbulent dispersion in the cross flow jet are found to change drastically depending on the thermal stratification. Especially, in the unstable condition, the vertical velocity fluctuation increases very rapidly at downstream of jet. The fluctuation velocity spectra and velocity-temperature cospectra along the jet centerline were obtained and compared. In the case of stable stratification, the heat flux cospectra changes Its sign from a certain point at the far field because of the restratification phenomenon. It is inferred that the main reason in the difference between the vertical heat fluxes is caused by the different length scales of the large eddy motions. The turbulent kinetic energy and scalar dissipation rates were estimated using partially non-isotropic and isotropic turbulent approximation. In the unstable case, the turbulent energy dissipation decreases more rapidly with the downstream distance than in the stable case.

• Journal of Korean Association for Spatial Structures
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• v.20 no.4
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• pp.149-158
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• 2020

The governing equation for a dome-type shallow spatial truss subjected to a transverse load is expressed in the form of the Duffing equation, and it can be derived by considering geometrical non-linearity. When this model under constant load exceeds the critical level, unstable behavior is appeared. This phenomenon changes sensitively as the number of free-nodes increases or depends on the imperfection of the system. When the load is a periodic function, more complex behavior and low critical levels can be expected. Thus, the dynamic unstable behavior and the change in the critical point of the 3-free-nodes space truss system were analyzed in this work. The 4-th order Runge-Kutta method was used in the system analysis, while the change in the frequency domain was analyzed through FFT. The sinusoidal wave and the beating wave were utilized as the periodic load function. This unstable situation was observed by the case when all nodes had same load vector as well as by the case that the load vector had slight difference. The results showed the critical buckling level of the periodic load was lower than that of the constant load. The value is greatly influenced by the period of the load, while a lower critical point was observed when it was closer to the natural frequency in the case of a linear system. The beating wave, which is attributed to the interference of the two frequencies, exhibits slightly more behavior than the sinusoidal wave. And the changing of critical level could be observed even with slight changes in the load vector.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.5
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• pp.346-352
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• 2016

This paper proposes an adaptive robust control method for an acrobot system in the presence of input disturbance. The acrobot system is a typical example of the underactuated system with complex nonlinearity and strong dynamic coupling. Also, disturbance can cause limit cycle phenomenon which appears in the acrobot system around the desired unstable equilibrium point. To minimize the effect of the disturbance, we apply a fuzzy disturbance estimation method for the swing-up and balancing control of the acrobot system. In this paper, both disturbance observer and controller for the acrobot system are designed and verified through mathematical proof and simulations.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.1
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• pp.89-95
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• 2015

A surge phenomenon cause noise and pulsations in a turbo compressor, which is an unstable operating regime. Because surge protection ensures a safe compressor operation, it is important to understand the physics of the surge phenomenon. In this study, the surge characteristics of a 250-hp class turbo-compressor were evaluated experimentally. The experimental parameters were the rotational speed, opening angles of the inlet guide vane and exit valve, and inlet pipe diameter and flow rates of the inlet gases. The results showed that the compressor surge was very sensitive to the gas flow rates, exit pressure, rotational speed, and bypass flow rates.

• Journal of Environmental Science International
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• v.4 no.2
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• pp.121-139
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• 1995

Based on the Results of Marine Meteorological and Oceanographical Observations (1966 -1987), the phenomenon of chimney is found as a candidate for the formation of the Japan Sea Proper Water (JSPW). The chimney phenomenon occurs twelve times Inuring 1966∼ 1987. The water types in the chimney denoting the deep convection are similar to those of the JSPW 0∼ 1℃ in potential temperature, 34.0∼34.1 ‰ in salinity and 68∼80 cl/t in potential thermosteric anomaly from the sea surface to the deep layer. The static stabilities in the chimney stations are unstable or neutral. This indicates that the winter time convection occurs. The JSPW sunken from the surface layer of chimney in winter spreads out under the Tsushima Warm Current area, following the isosteric surface of about 76 cl/t in Potential thermosteric anomaly. The formation of the deep water of the JSPW is mainly affected by the cooling of the sea surface than the evaporation of winds because the temperature and the salinity on the isoteric surface of about 76 cl/t in potential thermosteric anomaly ate cold and low The phenomenon of chimney occurred in here and there of the area in the north of 40" 30'N, west of 138" E. This suggests that the deep water of the JSPW is formed not in a limited area but probably in the overall region of the northern open ocean.

• Journal of Korean Association for Spatial Structures
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• v.4 no.2 s.12
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• pp.63-71
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• 2004

The structural space is gradually wide and is wanting agreeable environment by the requirement and necessity of people who lives modem stage. The building coincides with such requirements and is the high rise building actual circumstances which is doing ultra-large. The confirmed report of the technology to organize great merit is becoming currently considerably important issue in constructing a building field. Thus, this paper examine closely for nonlinear unstable taking a picture uneasiness height of prosperity considering to initial imperfection by a numerical method with a space frame structure of discrete system in large space structure. Based on previous investigation method, this paper induce nodal stiffness matrix of solid truss elements considering geometrical nonlinear using finite element method. In this paper, three types of space structure considered; i) 1-free node space structure, ii) 2-free node space structure, iii) multi-free node space structure. It apply the above examples to a nonlinear program, next, grasp the characteristic of an unstable conduct and the result was a clearing low.

• Korean Institute of Interior Design Journal
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• no.16
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• pp.106-115
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• 1998

Latest modernism shows general idea of phenomenal space through nature of place motility and experience sinceded from standard form and it has general idea of space. therefore this study presents the tension and the relaxation as a method looking for spatal change and value that in spatial experience. For the analysis of work the Conference Pavillon of Tadoa Ando in the furniture company in Vitra Fire Station of Zaha Hidid is analyzed in spatial experence. As the result the result the systematic straight mixture that has a superrimotion with long retangle which the space acts independently and is shown in the drawing of absolutism is formed and collapses theregularity of construction form by decomposition annexation mixing collision and ambivalence. The spatial experiance in this unstable form changes the grade of rank of space by opposite phenomenon by the change of spatial flowing the disorted and vague space that is occurred by superrimotion which has no direction and change of lirht and place and consolidates dynamic strain makes possible to construct against the gravity. And it also creates experienced space thatis reaxed with maintenance of organization of unity harmony, and stsbility.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.04a
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• pp.1-8
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• 2004

For the linearized differential algebraic equation of the nonlinear constrained system, exact initial values of the acceleration are needed to solve itself. It may be very troublesome to perform the inverse operation for obtaining the incremental quantities since the mass matrix contains the zero element in the diagonal. This fact makes the mass matrix impossible to be positive definite. To overcome this singularity phenomenon the mass matrix needs to be modified to allow the feasible application of predictor and corrector in the iterative computation. In this paper the proposed numerical algorithm based on the modified mass matrix combines the conventional implicit algorithm, Newton-Raphson method and Newmark method. The numerical example presents reliabilities for the proposed algorithm via comparisons of the 4th order Runge-kutta method. The proposed algorithm seems to be satisfactory even though the acceleration, Lagrange multiplier, and energy show unstable behaviour. Correspondingly, it provides one important clue to another algorithm for the enhancement of the numerical results.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1166-1171
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• 2004

The combustion instability acts as a serious obstacle for the lean premixed combustion of gas turbine and even causes the fatal damage to the combustor and whole system. In this experiment, the pressure fluctuation is highly related to the stabilizing position of flame and fuel injection location. The fuel injection location is connected with the convection time of the fresh mixture, which is important time scale to refresh the mixtures near the flame stabilization location. The flame is extremely unstable when the alternative stabilization occurs and bulk mode frequency (${\sim}10Hz$) of pressure fluctuation is observed in this condition. It was found that the convection time scale of the fresh reactant coincided with the time scale of the bulk mode fluctuation. Hence this phenomenon results from the local equivalence ratio change caused by the pressure fluctuation induced by thermo-acoustic effects.