• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.1022-1026
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• 2010

The different cleavage patterns of pYBamy59 plasmid isolated from E. coli $DH5{\alpha}$ and B. longum MG1 by the cell extract of B. longum MG1 suggested that the main reason for its low transformation efficiency was related to the restriction modification (R-M) system. To confirm the correlation between the R-M system and transformation efficiency, in vitro methylation and site-directed mutagenesis were performed in pYBamy59. Sequence analysis of pYBamy59 fragments digested by the cell extract of B. longum MG1 revealed that all fragments were generated by restriction of the sequence recognized by SacII endonuclease. When pYBamy59 from E. coli was methylated in vitro by CpG or GpC methyltransferase, it was protected from SacII digestion. Site-directed mutagenesis, which removed SacII sites from pYBamy59, or in vitro methylation of pYBamy59 showed 8- to 15-fold increases in the transformation efficiency over intact pYBamy59. Modification of the SacII-related R-M system in B. longum MG1 and in vitro methylation in pYBamy 59 can improve the transformation efficiency in this strain. The results showed that the R-M system is a factor to limit introduction of exogenous DNA, and in vitro modification is a convenient method to overcome the barrier of the R-M system for transformation.

• Information Systems Review
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• 제4권2호
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• pp.111-131
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• 2002

Strategic information systems assist organization with the significant opportunity to gain competitive advantage in executing its business plans and realizing its business goals. The universities establish long-range plan of information systems in an effort to provide just-in-time, useful, efficient and effective information services to their members such as professors, students, and staffs when carrying out various organizational activities. The university information systems started from the primitive computer-based application, extended to the administrative and educational e-transformation, even to the virtual university concept. Through an empirical analysis and complementary case examples, the current study carefully chose three Korean universities according to their chronological importance, that have undergone the information systems planning process years from 1995 to 2001. These universities then, were compared in terms of the information technology architecture: the information architecture, infra architecture, and information management architecture. These three areas of system were cross-examined under the consideration of the evolution of information technology architecture, and its impact on the development of university e-transformation. As part of a fulfillment plan to satisfy the needs of customers of current trend, it is evident that the university e-transformation provides a stepladder to highly competitive, first class university in the market, through achieving the educational advancement, research enhancement, and administrative efficiency. The result of the study analysis indicates that, of the three architectures examined, the weakness showed in the information management architecture. Therefore it is crucial for the university staffs, managers and professors to understand the significance of the successful implementation of the information system and its maintenance. Our study certainly will act as a catalyst for the promotion of the future university e-transformation projects.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.246-254
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• 2021

Environmental stresses are estimated to have reduced global crop yields of wheat by 5.5%. However, traditional approaches for the transfer of resistance to these stresses in wheat plants have yielded limited results. In this regard, genetic transformation has undoubtedly opened up new avenues to overcome crop losses due to various abiotic stresses. Particle bombardment has been successfully employed for obtaining transgenic wheat. However, most of these procedures employ immature embryos, which are not available throughout the year. Therefore, the present investigation utilized mature seeds as the starting material and used the calli raised from three Moroccan durum wheat varieties as the target tissue for genetic transformation by the biolistic approach. The pANIC-5E plasmid containing the SINA gene for drought and salinity tolerance was used for genetic transformation. To enhance the regeneration capacity and transformation efficiency of the tested genotypes, the study compared the effect of copper supplementation in the induction medium (up to 5 μM) with the standard MS medium. The results show that the genotypes displayed different sensitivities to CuSO₄, indicating that the transformation efficiency was highly genotype-dependent. The integration of transgenes in the T₀ transformants was demonstrated by polymerase chain reaction (PCR) analysis of the obtained resistant plantlets with primers specific to the SINA gene. Among the three genotypes studied, 'Isly' showed the highest efficiency of 9.75%, followed by 'Amria' with 1.25% and 'Chaoui' with 1%.

• Proceedings of the Korea Association of Information Systems Conference
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• 한국정보시스템학회 2005년도 추계학술대회 발표 논문집
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• pp.359-366
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• 2005

As the ubiquitous computing technology (uT) is prevailing, applying uT is more likely to transform the legacy way of doing business to a new way with the goals of ubiquitous computing: strategic use of information resources by using them everywhere, every time, with any devices for any services. These opportunities naturally require the provision to assess the degree of the transformation from the legacy IT-based e-business to the uT-based business. However, research about assessing the degree of u-transformation has been still very few. Moreover, even deciding what is ubiquitous or not is obscure. Hence, this paper aims to propose the methodology for assessing the degree of u-transformation oriented by the teleology of ubiquitous service, which intends to fully make use of uT in creating new business of next generation. Through the literature review, we developed the methodology to check whether the provided service is ubiquitous or not based on the capabilities that technologies have. and then, the methodology developed to assess the technical requirements that the uT should have when the transformation is considered through the focus group interview based on the literature review of the capability. A two-layered approach is introduced to assess not only the level of ubiquity but also the degree of u-transformation.

• The Journal of the Acoustical Society of Korea
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• 제22권4E호
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• pp.150-154
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• 2003

The distortion effects of microphones have been analyzed and compensated at mel-frequency feature domain. Unlike popular bias removal algorithms a linear transformation of mel-frequency spectrum is incorporated. Although a diagonal matrix transformation is sufficient for medium-quality microphones, a full-matrix transform is required for low-quality microphones with severe nonlinearity. Proposed compensation algorithms are tested with HTIMIT database, which resulted in about 5 percents improvements in recognition rate over conventional CMS algorithm.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.9-14
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• 2006

As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

• Journal of Engineering Education Research
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• 제14권5호
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• pp.67-74
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• 2011

The object of this study is to examine the process of the birth and transformation of civil engineering education as well as of modern civil engineers, called 'State noblesse', in the state engineer groups of France. For this, we will look into the background of founding the civil engineer corps and the civil engineering school, the transformation process of the civil engineering school into the applied school of Ecole Polytechnique, the activities of civil engineers in the industrialization process, and finally the organization and identity of the civil engineer group.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.747-758
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• 2002

The skin displays a highly active metabolism of polyunsaturated fatty acids (PUFA). Dietary deficiency of linoleic acid (LA), an 18-carbon (n-6) PUFA, results in characteristic scaly skin disorder and excessive epidermal water loss. Although arachidonic acid (AA), a 20-carbon (n6) PUFA, is metabolized via cyclooxygenase pathway into predominantly prostaglandin $E_2(PGE_2)$ and $PGF_{2{\alpha}}$, the metabolism of AA via the 15-lipoxygenase (15-LOX) pathway, which is very active in skin epidermis and catalyzes the transformation of M into predominantly 15S-hydroxyeicosatetraenoic acid (15S-HETE). Additionally, the 15-LOX also metabolizes the 18-carbon LA into 13S-hydroxyoctadecadienoic acid (13S-HODE), respectively. Interestingly, 15-LOX catalyzes the transformation of $dihomo-{\gamma}-linolenic$ acid (DGLA), derived from dietary gamma-linolenic acid, to 15S-hydroxyeicosatrienoic acid (15S-HETrE). These monohydroxy fatty acids are incorporated into the membrane inositol phospholipids which undergo hydrolytic cleavage to yield substituted-diacylglycerols such as 13S-HODE-DAG from 13S-HODE and 15S-HETrE-DAG from 15S-HETrE. These substituted-monohydroxy fatty acids seemingly exert anti-inflammatory/antiproliferative effects via the modulation of selective protein kinase C as well as on the upstream/down-stream nuclear MAP-kinase/AP-1/apoptotic signaling events.

• Journal of Institute of Control, Robotics and Systems
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• 제19권12호
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• pp.1145-1151
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• 2013

Kinematic modeling is a prerequisite for motion planning and the control of mobile robots. In this paper, we proposed a new method of kinematic modeling for a type of mobile robot based on differential motion transformation. The differential motion implies a small translation and rotation in three-dimensional space in a small time interval. Thus, transformation of the differential motion gives the velocity relationship, i.e., Jacobian between two coordinate frames. Since the theory of the differential motion transformation is well-developed, it is useful for the systematic velocity kinematic modeling of mobile robots. In order to show the validity for application of the differential motion transformation, we obtained velocity kinematic models for a type of exemplar mobile robot including spherical ballbots.

• Korean Journal of Mathematics
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• 제14권2호
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• pp.227-232
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• 2006

We study some dynamical properties in the context of bitransformation groups, and show that if (H,X,T) is a bitransformation group such that (H,X) is almost periodic and (X/H,T) is pointwise almost periodic $T_2$ and $x{\in}X$, then $E_x=\{q{\in}E(H,X){\mid}qx{\in}{\overline{xT}\}$ is a compact $T_2$ topological group and $E_{qx}=E_x(q{\in}E(H,X))$ when H is abelian, where E(H,X) is the enveloping semigroup of the transformation group (H,X).