• Journal of the Chungcheong Mathematical Society
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• v.5 no.1
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• pp.9-21
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• 1992

We define a certain subgroup $E_n$ (X, K, G) of the relative homotopy groups of a transformation group ${\sigma}_n$(X, K, G). The algebraic properties of these groups are examined.

• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1279-1287
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• 2012

We established an efficient transformation method for thermophile Geobacillus kaustophilus HTA426 using conjugative transfer from Escherichia coli of host-mimicking plasmids that imitate DNA methylation of strain HTA426 to circumvent its DNA restriction barriers. Two conjugative plasmids, pSTE33T and pUCG18T, capable of shuttling between E. coli and Geobacillus spp., were constructed. The plasmids were first introduced into E. coli BR408, which expressed one inherent DNA methylase gene (dam) and two heterologous methylase genes from strain HTA426 (GK1380-GK1381 and GK0343-GK0344). The plasmids were then directly transferred from E. coli cells to strain HTA426 by conjugative transfer using pUB307 or pRK2013 as a helper plasmid. pUCG18T was introduced very efficiently (transfer efficiency, $10^{-5}-10^{-3}\;recipient^{-1}$). pSTE33T showed lower efficiency ($10^{-7}-10^{-6}\;recipient^{-1}$) but had a high copy number and high segregational stability. Methylase genes in the donor substantially affected the transfer efficiency, demonstrating that the host-mimicking strategy contributes to efficient transformation. The transformation method, along with the two distinguishing plasmids, increases the potential of G. kaustophilus HTA426 as a thermophilic host to be used in various applications and as a model for biological studies of this genus. Our results also demonstrate that conjugative transfer is a promising approach for introducing exogenous DNA into thermophiles.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.40-49
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• 1984

To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM $CaCl_2$ before heat shock and the treatment of $CaCl_2$ was essential step in the process of E. coli transformation. 2. The highest transformation frequency was observed in the treatment of heat shock at $42^{\circ}C$ for 4 min. or $37^{\circ}C$ for 6 min., but the prolonged heat shock resulted a decreased transformation frequency. 3. Treatments of ice incubation at $0^{\circ}C$ for 45 min. before heat stocks or at $0^{\circ}C$ for 30min. after heat shock resulted an increased transformation frequency. 4. There was a linear relationship between DNA concentration and transformation frequency at the concentration of $8{\times}10^3$ recipient cells. The highest transformation frequency reached in carte of 7 mcg of donor DNA, but above 1 mcg of DNA concentration, transformation frequency was not remarkably increased. Addition of donor DNA just after the treatment of $CaCl_2$ was the best. 5. The best condition of gene expression at $37^{\circ}C$ were 40min. for TC-resistant gene and 100min. for AP-resistant gene. TC-resistant gene was higher in the transformation frequency and faster in the gene expression time than AP-resistant gene. In these results, the best conditions for the transformation of E. coli C 600 with pBR322 DNA were: treatment with 100mM $CaCl_2$, ice incubation at $0^{\circ}C$ for 45 min, heat shock at $42^{\circ}C$ for 4 min., 30 min. of ice incubation and incubation at $37^{\circ}C$ for 100min. for gene expression in that order.

• Molecules and Cells
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• v.24 no.3
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• pp.445-451
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• 2007

Translation initiation factor 4E (eIF4E) is a key regulator of protein synthesis. Abnormal regulation of eIF4E is closely linked to oncogenic transformation. Several regulatory mechanisms affecting eIF4E are discussed, including transcriptional regulation, phosphorylation and binding of an inhibitor protein. However it is not clear how the level of eIF4E protein is regulated under basal conditions. Here we demonstrate that Diap1 (Drosophila Inhibitor of Apoptosis Protein), a cell death inhibitor, binds directly to eIF4E and poly-ubiquitinates it via its E3 ligase activity, promoting its proteasome-dependent degradation. Expression of Diap1 caused a reduction of Cyclin D1 protein level and inhibited the growth stimulation induced by overexpression of eIF4E. Taken together, our results suggest that the level of eIF4E protein is regulated by Diap1, and that IAPs may play a role in cap-dependent translation by regulating the level of eIF4E protein.

• Korean Journal of Geomatics
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• v.4 no.1
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• pp.31-37
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• 2004

The aim of this research is comparing the existing approximation models (e.g. Affine Transformation and Direct Linear Transformation) with Rational Function Model as a substitute of rigorous sensor model of linear array scanner, especially push-broom sensor. To do so, this research investigates the mathematical model of each approximation method. This is followed by the assessments of accuracy of transformation from object space to image space by using simulated data generated by collinearity equations which incorporate or depict the physical aspects of linear array sensor.

• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.345-348
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• 1985

Recombinant chimeric plasmid constructed with Xba I digested pUBl10 and -pE194 was transformed by polyethylene glycol induced protoplast transformation system into Bacillus subtilis BR 151 on the mannitol regeneration media, and two genes of antibiotics resistance were expressed simultaneously in the transfromant. Transformation frequency of the recombinant plasmid was 6.5 $\times$ 10$^{-5}$ on the mannitol regeneration agar plate containing neomycin and erythromycin. The replication of recombinant plasmid in the recipient cells was confirmed by the alkaline extraction method and agarose gel electrophoresis.

• Information Systems Review
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• v.10 no.2
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• pp.269-289
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• 2008

It is very difficult problem to estimate and evaluate the performance of e-government system which scope and size are large and its effectiveness can not be seen shortly but reveals after several years. It is because the previous offline processes can not be transformed to online ones fully and shortly. For such e-transformation cases, the performance evaluation model should be adjusted and modified gradually as time passes. This paper propose new EEM(E-transformation Evaluation Model) model and methodology to evaluate G2B system that is one of large e-government project. EEM model can derive monetary value of e-transformatized business process areas(online areas). It also estimate the expected effect of offline area that is not yet transformed to online. EEM model consists of standard model, verification model and estimation model with some variables such as evaluation year, evaluation area and data type. By using survey data and database data together it can validate the correctness of the model and derive the effect of the system introduction. This paper also propose EEM evaluation methodology consisting of 5 stages and 10 sub processes to evaluate online and offline effect efficiently. To show the usefulness of this study, we evaluate the performance of Korea G2B system named KONEPS which is famous as a successful e-government case in the world by using the proposed model and methodology. The proposed model and methodology can be applied to different similar areas including e-government projects and large scale information system introduction in private sectors. This study can be also used for establishing appropriate policies about e-government project and informatization issues.

• Mycobiology
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• v.45 no.2
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• pp.84-89
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• 2017

Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.

• Journal of Information Technology Applications and Management
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• v.15 no.3
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• pp.227-242
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• 2008

The purpose of this study is to identify the critical factors for the successful implementation of e-business in the public sector. The paper reports on a case study of the 'Onbid' asset-management system developed by the Korean Asset Management Corporation (KAMCO). 'Onbid' system is an e-marketplace for trading in public assets, including the disposition by public sale of real estate. Through this case study, the paper: (i) explores the changes in organizational culture that are required for successful e-transformation in organizations of public sectors; and (ii) identifies the critical success factors for the implementation of e-business in the public sector.

• The KIPS Transactions:PartA
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• v.9A no.1
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• pp.37-44
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• 2002

Most researches of transforming sequential programs into parallel programs have been based on the loop structure transformation method. However, most programs have implicit interprocedure parallelism. This paper suggests a way of extracting parallelism from the loops with procedure calls using the data dependency elimination method. Most parallelization of the loop with procedure calls have been conducted for extracting parallelism from the uniform code. In this paper, we propose interprocedural transformation, which can be apply to both uniform and nonuniform code. We show the examples of uniform, nonuniform, and complex code parallelization. We then evaluated the performance of the various transformation methods using the CRAY-T3E system. The comparison results show that the proposed algorithm out-performs other conventional methods.