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Production of curdlan with agro-industrial byproduct by Agrobacterium sp. ATCC 31749

  • 정대영;김현숙;서형필;이남규;김지모;이진우
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.251-254
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    • 2000
  • Effect of carbon sources including agro-industrial byproduct on cell growth and production of curdlan by Agrobacterium sp. ATCC 31749 was investigated. Maximal production of curdlan was obtained when the carbon source was sucrose. The conversion rate of curdlan from 2% (w/v) sucrose was 59%. Glucose, mannose and maltose were also found to be good carbon sources for production of curdlan. Production of curdlan increased up to 3% (w/v) glucose as the carbon source and then decrease as the concentration of glucose increased. The major components of agro-industrial byproduct (AIB) were glucose, maltose, and maltose, and maltotriose. Agrobacterium sp.ATCC 31749 utilized up to 25% (v/v) AIB and produced curdlan with 29.8g/1.

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Antioxidant Effects of Isoflavones from the Stem Bark of Cudrania tricuspidata

  • Lee, Jin-Hwan;Lee, Byong-Won;Kim, Jin-Hyo;Seo, Woo-Duck;Jang, Ki-Chang;Park, Ki-Hun
    • Journal of Applied Biological Chemistry
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    • 제48권4호
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    • pp.193-197
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    • 2005
  • Isoflavones 4'-O-methylalpinumisoflavone (1) and 5, 7, 4'-trihydroxy-6,8-diprenylisoflavone (2) were isolated from stem bark of Cudrania tricuspidata (Carr.) Bureau. These compounds were the first reported from this plant. Antioxidant activities 1 and 2 were evaluated by measuring their ability to scavenge DPPH and ABTS radicals. Diprenylisoflavone 2 showed strong scavenging activity against ABTS ($IC_{50}\;=\;16.3\;{\mu}M$), three-fold higher compared to genistein, which do not possess prenylated group which indicates strong scavenging activity against ABTS radical of isoflavone 2 was due to prenyl group.

Stereospecific Synthesis of the (2R,3S)- and (2R,3R)-3-Amino-2-hydroxy-4-phenylbutanoic Acids from D-Glucono-δ-lactone

  • Lee, Jin Hwan;Kim, Jin Hyo;Lee, Byong Won;Seo, Woo Duck;Yang, Min Suk;Park, Ki Hun
    • Bulletin of the Korean Chemical Society
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    • 제27권8호
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    • pp.1211-1218
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    • 2006
  • The enantiomerically pure (2R,3S)- and (2R,3R)-3-amino-2-hydroxy-4-phenylbutanoic acids (AHPBA) 1 and 3 are readily obtained from D-glucono-a-lactone. Both AHPBAs are the structural key units of KMI derivatives which are the potent inhibitors of BACE 1 ($\beta$-secretase) and HIV protease. Additionally, the obtained AHPBAs 1 and 3 are converted to dipeptides of bestatin stereoisomers 2 and 4.

Gene Expression Profiling at Early Stage of Head Regeneration in the Earthworm(Perionyx excavatus) using Expressed Sequence Tags

  • Cho, Sung-Jin;Lee, Myung-Sik;Eunsik Tak;Lee, Jong-Ae;Park, Bum-Joon;Cho, Hyun-Ju;Moon, Joo-Sik;Park, Soon-Cheol
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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    • pp.143-143
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    • 2003
  • Among all animal species, a few have the remarkable capacity to regenerate a missing part of their body after amputation. The early process of epimorphic regeneration in which dedifferentiation and cell proliferation are involved, provides a useful model to investigate the mechanism of normal development as well as differentiation. To better understand early stage of head regeneration, we have generated 5'-end sequence of 1,592 expressed sequence tags (ESTs) from cDNA library of regenerating tissue. (omitted)

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