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Citric Acid Production and Scale-up in Dual Hollow Fiber Bioreactor (이중실관 생물 반응기에서의 구연산 생산과 Scale-up)

  • 장호남;지동진;심상준
    • Membrane Journal
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    • v.2 no.2
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    • pp.122-128
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    • 1992
  • A study on the citric acid production was performed in various size dual hollow fiber bioreactors with immobilized Aspergillus niger (KCTC 1232). The final dry cell mass density reached 300g/l based on the space volume available for cell growth. Under air and oxygen aeration the volumethe productivity reached 0.63 and 1.02g/l.h, which cormsponded to 10 and 16 fold over those of batch fermentation, respectively. The initial pH of the medium was a critical factor and the lower value resulted in higher citric acid yield. The increase in the feeding rate of medium or the number of reactor unit resulted in the improvement of the productivity due to higher consumption rate of substrate.

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A Constitutive Inulase from a Bacillus (Bacillus 균이 생산하는 구성효소로서의 이눌라아제)

  • Uhm, Tai-Boong;Hong, Jai-Sik;Sohn, Hee-Suk;Park, Mun-Kuk;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.28 no.3
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    • pp.131-136
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    • 1985
  • Recently, we investigated characteristics of a bacterium which has never been reported as an inulin hydrolyzing microorganism. Several properties of the isolated microorganism were aerobic, rod typed, spore forming and Gram positive. According to the Bergey's manual, this bacterium tentatively appeared to be Becillus subtilis. This bacterium produced inulase constitutively in the media containing giucose, sucrose or cellulose without inulin as a sole carbon source. Also, inulase activities of the bacterium per unit culture volume showed 1.1 unit/ml comparable to 0.9 unit/ml of Kluyveromyces fragilis with relatively short culture time.

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A Suggestion for the Worth Evaluation of Warm Air and the Allocation Methodology of Heating Cost (온기의 가치평가 및 난방비 배분방법론 제안)

  • Kim, Deok-Jin
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.20 no.10
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    • pp.654-661
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    • 2008
  • Our government will make a plan regulating the cooling limit temperature of the summer season to 26 degree and the heating limit temperature of the winter season to 20 degree for energy saving. Where, the key point of this politic pursuit can be the charge system on heating and cooling cost. Currently, the heating and cooling cost are charged as much as the volume or heat flow rate regardless of the high and low room temperature. However, we have suggested new cost estimating methodology as a worth method in the precedent study, and preformed the worth evaluation and cost allocation of electricity and heat on various cogenerations. In this study, we applied the suggested worth method to four kind of warm air, and preformed the worth evaluation and cost allocation of each warm air. As a result, the more energy a customer saved, the more heating unit cost decreased, and the more energy a customer consumed, the more heating unit cost increased. From this analysis, we hope that the suggested worth methodology can offer a theoretical basis to the politic pursuit of government, and induce the spontaneous energy saving of consumers.

A Study on the Estimation of Total Amounts of Blasted Rock by Detonator Volume used in the Blasting (뇌관당 파쇄암량을 고려한 발파작업수량 산정 연구)

  • 김민규;안명석
    • Explosives and Blasting
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    • v.21 no.1
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    • pp.41-47
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    • 2003
  • A large scale blasting is necessary for the construction or road, harbor or ground foundation of building and it is common that the blasting work is performed by a specialist subcontracted from the construction company who is originally responsible for the project. Sometimes the latter do not agree with the former in total amount of blasted rock. They try to find out real work amount as precisely as possible. The estimation is sometimes carried out by an entrusted person when it is impossible to come to an agreement with each other. There are several methods in estimating the blasted rock volume; a calculation by prescribed equivalents of explosive before construction, a calculation by specific charge per unit volume of rock, and a calculation by rock volume per detonator. In this study, the last method is reviewed and recommended as most reliable one.

Hatching Rate of Pacific Cod (Gadus macrocephalus) in a Large Volume Hatching Rate of Pacific Cod (Gadus macrocephalus) in a Large Volume of the Hatching Jar (대용량 부화기를 이용한 대구 Gadus macrocephalus 수정란부화율)

  • Gwak, Woo-Seok;Han, Dong-Hoon;Lee, So-Gwang
    • Korean Journal of Ichthyology
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    • v.22 no.3
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    • pp.195-200
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    • 2010
  • A large volume of hatching jar was tested whether it is an effective to hatch fertilized eggs of Pacific cod. The volume of hatching jar did not show any significant differences in survival of the fertilized eggs; 48.3% for the jar of 15 L and 50.4% for that of 42 L. Survival rate of the fertilized eggs in a large volume of hatching jar ranged from 40.0 to 71.2%, which was higher than those of conventional small circular tank. A higher survival could be achieved even though stocking density of fertilized eggs was as high as 5,000 mL/jar. Consequently, the tested hatching jar allowed for incubation of a greater number of eggs with higher survival rate in a much smaller space. In addition, it reduces costs and manpower, and requires a relatively small amount of water per individual unit (6,700 mL/min), and provides a way to incubate multiple rearing groups in a quarantine environment.

Evaluation of intaglio surface trueness, wear, and fracture resistance of zirconia crown under simulated mastication: a comparative analysis between subtractive and additive manufacturing

  • Kim, Yong-Kyu;Han, Jung-Suk;Yoon, Hyung-In
    • The Journal of Advanced Prosthodontics
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    • v.14 no.2
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    • pp.122-132
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    • 2022
  • PURPOSE. This in-vitro analysis aimed to compare the intaglio trueness, the antagonist's wear volume loss, and fracture load of various single-unit zirconia prostheses fabricated by different manufacturing techniques. MATERIALS AND METHODS. Zirconia crowns were prepared into four different groups (n = 14 per group) according to the manufacturing techniques and generations of the materials. The intaglio surface trueness (root-mean-square estimates, RMS) of the crown was measured at the marginal, axial, occlusal, and inner surface areas. Half of the specimens were artificially aged in the chewing simulator with 120,000 cycles, and the antagonist's volume loss after aging was calculated. The fracture load for each crown group was measured before and after hydrothermal aging. The intaglio trueness was evaluated with Welch's ANOVA and the antagonist's volume loss was assessed by the Kruskal-Wallis tests. The effects of manufacturing and aging on the fracture resistance of the tested zirconia crowns were determined by two-way ANOVA. RESULTS. The trueness analysis of the crown intaglio surfaces showed surface deviation (RMS) within 50 ㎛, regardless of the manufacturing methods (P = .053). After simulated mastication, no significant differences in the volume loss of the antagonists were observed among the zirconia groups (P = .946). The manufacturing methods and simulated chewing had statistically significant effects on the fracture resistance (P < .001). CONCLUSION. The intaglio surface trueness, fracture resistance, and antagonist's wear volume of the additively manufactured 3Y-TZP crown were clinically acceptable, as compared with those of the 4Y- or 5Y-PSZ crowns produced by subtractive milling.

Volume of Water Storage and Evapotranspiration by Inserted Materials at a Reservoir of Porous Grass Block (저수형 잔디블록 저수조 내 충진재료에 따른 저수량 및 초종별 증발산량)

  • Han, Seung-Ho;Choi, Joon-Soo;Yang, Geun-Mo;Yang, Byoung-E;Kang, Jin-Hyoung;Kim, Won-Tae
    • Journal of the Korean Institute of Landscape Architecture
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    • v.34 no.5 s.118
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    • pp.76-83
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    • 2006
  • The purpose of this study was to investigate the performance of porous grass block. For the investigation, Festuca arundinacea and Zoysia japonica 'Zenith' were planted, and the volume of evapotranspiration and remains were examined based on different materials in the water tank in the experiment of Festuca arundinacea, the volume of water storage of treatment with perlite ($10.84{\iota}/m^2$) was higher than that with drainage ($7l/m^2$). The difference between the two was $3.84/m^2$. The drainage treatment without water storage capacity showed the higher degree of dryness in turf grass. The volume of evapo-transpiration of treatment with perlite was the highest (21.57mm/week). The volume of evapotranspiration of treatment with sand was 19.57mm/week, and with treatment with drainage was 18.24mm/week. Based on the measured volume of daily evapotranspiration of $2.60{\sim}3.08mm\;d^{-1}$, it was determined that the unit with water storage capacity would store water of one to two days usage compared to unite without such storage capacity. In the experiment of Zoysia japonica 'Zenith', the volume of water storage of treatment with perlite was $10.77l/m^2$ which was similar to the former experiment. The volume of evapotranspiration of treatment with perlite and sand were 21.64mm/week and 20.64mm/week, respectively. In case of airtight water tank, the volume was measured as 22.06mm/week. Each treatment has no notable difference in the volume of evapotranspiration. In conclusion, from the investigation in this study, porous grass block with water tank was found to be effective in plant growth under low irrigation. As the ecological area ratio and vegetated porous pavement have became more emphasized, additional study of rain infiltration and reservoir effect are needed in the future.

Micro/Millimeter-Wave Dielectric Indialite/Cordierite Glass-Ceramics Applied as LTCC and Direct Casting Substrates: Current Status and Prospects

  • Ohsato, Hitoshi;Varghese, Jobin;Vahera, Timo;Kim, Jeong Seog;Sebastian, Mailadil T.;Jantunen, Heli;Iwata, Makoto
    • Journal of the Korean Ceramic Society
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    • v.56 no.6
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    • pp.526-533
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    • 2019
  • Indialite/cordierite glass-ceramics demonstrate excellent microwave dielectric properties such as a low dielectric constant of 4.7 and an extremely high quality factor Qf of more than 200 × 103 GHz when crystallized at 1300℃/20 h, which are essential criteria for application to 5G/6G mobile communication systems. The glass-ceramics applied to dielectric resonators, low-temperature co-fired ceramic (LTCC) substrates, and direct casting glass substrates are reviewed in this paper. The glass-ceramics are fabricated by the crystallization of glass with cordierite composition melted at 1550℃. The dielectric resonators are composed of crystallized glass pellets made from glass rods cast in a graphite mold. The LTCC substrates are made from indialite glass-ceramic powder crystallized at a low temperature of 1000℃/1 h, and the direct casting glass-ceramic substrates are composed of crystallized glass plates cast on a graphite plate. All these materials exhibit excellent microwave dielectric properties.

A sutdy on the District Unit Design for CO2 Reduction of Transportation (교통부문 CO2 저감을 위한 지구단위설계 방법에 관한 연구)

  • Jin, Jang-Won;Park, Min-Kwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.3
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    • pp.1370-1376
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    • 2012
  • This study tried to analyze $CO_2$ emission volume as green-house gases by application of land use patterns and transport policies in District Unit Design. It is postulated a Toy network and various scenarios which are combined land use patterns and transport policies for analyzing $CO_2$ gas reduction. As results, this study shows best District Unit Design technique is the policy that develop mid block and introduction of car free zone to inner 2 way streets. Worst design technique is the policy that make hierarchical network and introduction of access control to outer roads that have been known as a best road policy till nowadays. Therefore, we need more carefully introduce design technique for reduction of $CO_2$ in District Unit.

Design and Effectiveness Analysis of prefabricated Storage-type infiltration facility (조립식 저류형 침투시설의 설계 및 공간적용 효과분석)

  • Lee, Tae-Goo
    • KIEAE Journal
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    • v.16 no.6
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    • pp.103-108
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    • 2016
  • Purpose: This study has developed economical and environmentally friendly storage type infiltration facilities that securing storage space inside the infiltration facility. It focused on preventing flooding rainfall as well as securing more groundwater through rainwater infiltration that is valuable for the dry season. In addition, this study compares the installation cost of the storage-type infiltration facility to the cost of the conventional rainwater management facilities to demonstrate the economic efficiency of the storage-based infiltration facility. Method: Unit infiltration of this facility is calculated and when it was applied to a certain capacity, the amount of countermeasures are proposed in case study. Result: Unit infiltration of it is $0.2541m^3/hr$ and un it Temporary storage of it is $1.054m^3/m$. As a result, the infiltration effect of this facility is $1.306m^3/hr$. The cost was approximately 30% reduction in time to apply the storage type infiltration facility as compared with the case to apply the existing penetration of the facilities. Since the penetration of the existing facilities is smaller than that and it has much securing volume to process the same the amount of countermeasures. Therefore, it is determined that the cost significantly increases in material cost part. On the other hand, storage type infiltration facility is installed a small quantity because Unit Temporary storage and infiltration are bigger than that. So, it occurred to reduce material and installation costs.