• The Plant Pathology Journal
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• 제28권4호
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• pp.341-356
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• 2012

Shiitake (Lentinula edodes) is the most economically important cultivated mushroom, but yields are impacted by its competitor, Trichoderma spp. We previously found two unidentified Trichoderma species growing in bedlogs and sawdust shiitake media in Korea. Here, we identify and re-describe those two species based on molecular sequence data, morphology, and culture characteristics. Well-supported clades based on phylogenetic analyses of internal transcribed spacer, translation elongation factor 1-${\alpha}$, and RNA polymerase subunit II sequences grouped one of the unidentified Trichoderma spp. with Hypocrea pseudogelatinosa and the other with Hypocrea pseudostraminea, and their morphologies matched well with the original descriptions of the two Hypocrea species. This study reports the first phylogenetic analyses of H. pseudogelatinosa and Japanese strains of H. pseudostraminea. Based on the phylogenetic results, we redescribed these two species using modern taxonomic concepts in Hypocrea/Trichoderma.

• 농업생명과학연구
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• 제43권4호
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• pp.21-31
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• 2009

Dipterans are excellent insects to serve as forensic indicators to determine the postmortem interval of a human corpse. Therefore, we undertook a study to identify the major dipterans that are attracted to and develop in vertebrate carcasses. To determine what dipterans occur in Korea, chicken carcasses were placed in various habitats including the forest, open field, stream bank, greenhouse, rooftop, rice paddy, Chinese cabbage field, vacant hut, and pond at different times of the year. The dipterans were collected at various time intervals after placement in each habitat until the end of decomposition and identified. Our results showed that 49 identified and unidentified dipteran species from 36 genera in 19 families were collected from the carcasses. Habitat placement influenced the number of species isolated from the carcasses. For example, 39 species were collected from the forest habitat, 28 species from the open field, stream bank, or greenhouse each, 16 species from a vacant home, and 12 species from the pond. The dipteran species that were collected from all habitats were Phaenicia sericata, Lucilia illustris, and Lucilia sp. in the family Calliphoridae and Boettcherisca peregrine in the family Sarcophagidae. Other dipteran species that were isolated depended on carcass placement that is, fewer dipteran species were observed from carcasses in the backpack and much more species were recorded from unburied carcasses than buried carcasses. P. sericata, L. illustris, Lucilia sp., B peregrine and an unidentified sarcophagid species were observed year round, but some dipterans showed seasonal differences.

• 대한본초학회지
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• 제26권1호
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• pp.97-101
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• 2011

Objectives : The purpose of this research is to identify the actual origin species imported as Aquilaria malaccensis from Indonesia. Methods : After producing the permanent preparat by cutting off the specimen thinly, we observed its characteristics. Results : The imported ones have not interxylary parenchyma, so none is in the genus aquilaria. Since the aliform parenchyma is mostly observed around the vessel pore, they are in the genus gonystylus, and the rest have no aliform parenchyma, so their specimen is unidentified. Conclusion : The imported as A. malaccensis are not in the genus aquilaria, but most of them are in the genus gonystylus and, for the rest, the specimen is unidentified.

• The Korean Journal of Ecology
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• 제21권4호
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• pp.345-349
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• 1998

thirteen dictyostelid cellular slime molds were isolated from forest soils, cattle dung and manure inisland Ulneungdo, South Korea. They were ten species including Dictyostelium aureo-stipes var. aureo-stipes, D. brefeldianum, D. macrocephalum, D. caudabasis, D. sphaerocephalum, D. giganteum, D. crassicaule, Polysphondylium pallidum, P. violaceum and P. tenuissimum as well as three species unidentified. D. aureo-stipes var. aureo-species of genus Polysphondylium distributed widely. It was thought that species diversi쇼 was considerabley higher.

• 한국어병학회지
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• 제18권2호
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• pp.119-124
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• 2005

Two species of myxosporean parasites - Parvicapsula anisocaudata and an unidentified myxosporean were found in the lumina of renal tubules and the tubular epithelium, respectively, from cultured olive flounder, Paralichthys olivaceus in Korea. The latter was also seen in interstitial tissue of spleen and interrenal gland of the head kidney. Group of pseudoplasmodia of P. anisocaudata were firmly attached on the epithelium of renal tubules through pseudopodia. In the renal tubule epithelium, a group of unidentified myxosporean trophozoites, which were 2-3 times larger than intraluminal trophozoites of P. anisocaudata, was observed. The parasites being burst out into the lumen was occasionally encountered with partial break of the epithelium. Although infection of P. anisocaudata and unidentified myxosporean parasites did not induce any cellular reaction of the host, occlusion of renal tubules and rupture of renal epithelium would impact negatively on the renal functions of severely infected fish.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1553-1560
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• 2022

A Gram-stain-negative, rod-shaped bacterial strain, JC4^T, was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4^T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133^T. The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4^T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4^T contained summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and iso-C_15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4^T was determined to be 42.44%. The above polyphasic evidences support that strain JC4^T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4^T (= KCTC 92230^T = LMG 32715^T).

• Animal cells and systems
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• 제13권3호
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• pp.323-329
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• 2009

We analyzed partial sequences of cytochrome b (cyt-b), a mitochondrial DNA (mtDNA) gene, to determine the genetic relationships between six horsehead fish species: Branchiostegus japonicus, Branchiostegus albus, Branchiostegus auratus, Branchiostegus argentatus, Branchiostegus wardi, and an unidentified Branchiostegus species. The specimens were collected in Korea, China, Japan, and Vietnam. We compared their molecular phylogenetic relationships inferred from mtDNA cyt-b sequences with an osteological analysis. The unidentified species, B. sp., was similar to B. albus in terms of the lack of triangular silver-white dot at the posterior region of eyes (vs. large one present in B. japonicus), but was also similar to B. japonicus in terms of the presence of a straight-shaped first hemal spine (vs. a curve-shaped hemal spine in B. albus). Analysis of the mtDNA cyt-b sequences indicated that the smallest estimated sequence divergence was between the B. japonicus and B. sp. (0.70-0.94%), whereas the largest difference was between B. auratus and B. argentatus (23.06-23.36%). Both the maximum parsimony and maximum likelihood trees showed that the B. sp. was closely clustered with B. japonicus, and that B. auratus was most distant from the other species. When comparing the osteological characters, UPGMA tree showed that the B. japonicus and B. sp. were the most closely clustered species, and B. auratus was the most distantly clustered fish relative to the other species. The shape of the nasal, otolith and first hemal spine was informative for distinguishing B. auratus from the other species. These osteological differences were consistent with the differences in mtDNA.

• 해양환경안전학회지
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• 제19권4호
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• pp.309-314
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• 2013

인공용승류 해역에서 난자치어의 종조성 및 시공간 변동을 파악하기 위하여 2012년 6월 13일, 9월 21일 및 25일에 난자치어 네트를 이용하여 조사하였다. 조사기간 동안 출현한 어란은 4,849 ind./$1,000m^3$, 자치어는 641 ind./$1,000m^3$이였다. 출현한 어란 중 가장 우점한 종은 멸치(Engraulis japonicus)로써 총 출현량의 약 57.5 %를 차지하였다. 그 외 미동정(Unidentified) 어란 I, II, III, IV이 출현하였다. 자치어는 청베도라치(Pictiblennius yatabei)가 가장 우점하였으며, 그 다음으로 돛양태류(Repomucenus sp.A, sp.B), 고등어(Scomber japonicus), 멸치(Engraulis japonicus), 전어(Konosirus punctatus) 등의 순으로 나타났다. 그리고 미동정(Unidentified) 자치어 I, II가 출현하였다. 대조구 해역에서는 어란은 멸치, 자치어는 돛양태류가 출현하였다. 시기별 변동에서는 어란 및 자치어 모두 7월에 가장 많았으며, 8월에 가장 낮았다. 정점별 변동에서는 인공용승이 일어나는 인근 정점인 2, 3번에서 어란 및 자치어의 출현량이 가장 많았다. 대조구 해역과의 비교하여 정점당 약 4배 이상 많은 어란 및 자치어가 출현하였다. 인공용승에 의한 영양염류 및 먹이생물의 증가로 인하여 어란 및 자치어의 출현량이 증가한 것으로 판단된다.

• 수산해양기술연구
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• 제55권3호
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• pp.243-251
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• 2019

The ichthyoplanktons were sampled by Bongo net to study the distribution of the eggs and larvae in the West coastal waters of Korea during the study period (February, May, August and October of 2017). Collected fish eggs were divided into Engraulis japonicus and unidentified species. The most dominant species, Engraulis japonicus accounted for 79.52% of the total fish egg, which was followed by unidentified species (20.48%). The total of 36 taxa in fish larvae were identified based on morphological and molecular characteristics. Fish larvae were dominant by Gobiidae species. 48.30% of the total catch in abundance, which was followed by Engraulis japonicus (31.86%). These two taxa composed 80.16% of the total collected fish larvae. The seasonal diversity index of species from the collected fish larvae in the West coastal waters in 2017 was 0.625-1.883 and the evenness index was 0.380-0.865. In addition, dominance index was 0.618-0.920 and richness was 0.573-3.189.

• BMB Reports
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• 제39권4호
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• pp.361-370
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• 2006

Genetic variation and molecular phylogeny of 22 taxa representing 14 extant species and 3 unidentified taxa of Boesenbergia in Thailand and four outgroup species (Cornukaempferia aurantiflora, Hedychium biflorum, Kaempferia parviflora, and Scaphochlamys rubescens) were examined by sequencing of 3 chloroplast (cp) DNA regions (matK, psbA-trnH and petA-psbJ). Low interspecific genetic divergence (0.25-1.74%) were observed in these investigated taxa. The 50% majority-rule consensus tree constructed from combined chloroplast DNA sequences allocated Boesenbergia in this study into 3 different groups. Using psbA-1F/psbA-3R primers, an insertion of 491 bp was observed in B. petiolata. Restriction analysis of the amplicon (380-410 bp) from the remaining species with Rsa I further differentiated Boesenbergia to 2 groupings; I (B. basispicata, B. longiflora, B. longipes, B. plicata, B. pulcherrima, B. tenuispicata, B. thorelii, B. xiphostachya, Boesenbergia sp.1 and Boesenbergia sp.3; phylogenetic clade A) that possesses a Rsa I restriction site and II (B. curtisii, B. regalis, B. rotunda and Boesenbergia sp.2; phylogenetic clade B and B. siamensis; phylogenetic clade C) that lacks a restriction site of Rsa I. Single nucleotide polymorphism (SNP) and indels found can be unambiguously applied to authenticate specie-origin of all investigated samples and revealed that Boesenbergia sp.1, Boesenbergia sp.2 and B. pulcherrima (Mahidol University, Kanchanaburi), B. cf. pulcherrima1 (Prachuap Khiri Khan) and B. cf. pulcherrima2 (Thong Pha Phum, Kanchanaburi) are B. plicata, B. rotunda and B. pulcherrima, respectively. In addition, molecular data also suggested that Boesenbergia sp.3 should be further differentiated from B. longiflora and regarded as a newly unidentified Boesenbergia species.