• Title/Summary/Keyword: Uniaxial stretch

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Effects of Mechanical Stimuli on the Cell Proliferation and Collagen Production on the Micropatterned Substrate

  • Park Su-A;Kim In-Ae;Kim Chong-Rak;Shin Ji-Won;Heo Su-Jin;Hwang Young-Mi;Kim Dong-Hwa;Shin Jung-Woog
    • Biomedical Science Letters
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    • v.12 no.2
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    • pp.119-125
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    • 2006
  • In relation to the tissue engineering, the cellular responses to the morphology of the scaffold surface are interesting topics. Human ligament fibroblasts (HLFs) were cultured on the micrpatterned silicone substrates subjected to cyclic stretch to simulate ligament motion. Groove and ridge width of silicone substrates was 10/50, 20/50, 20/10, and 20/20 ${\mu}m$ (groove/ridge ${\mu}m$) with a depth of $3{\mu}m$. Strain was applied over two days for 4 hours per day with a frequency of 0.5 Hz with the magnitudes of 4 or 8%. The purpose of this study was to evaluate ligament fibroblast alignment and cellular responses in relation to the pattern of microgrooved surface and stretching magnitude. Ligament fibroblasts in the microgrooved surface were elongated and aligned parallel to the microgrooves under no stretch. Uniaxial cyclic stretch induced cellular activities and their orientation rise in cellular response and the cells showed alignment and elongation perpendicular to the direction of the stretch. Biochemical analyses showed that the best cellular response was found on the $20/50{\mu}m$ under 8% stretch. The surface morphology and mechanical stretching were found to contribute to increase of proliferation, collagen production.

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Measurement of Mechanical Material Properties of Rubber Compounds Sampled from a Pneumatic Tire (타이어에서 채취한 고무배합물의 기계적 물성 측정)

  • 김용우;김종국
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 2002.04a
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    • pp.404-409
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    • 2002
  • Pneumatic tires usually contain a variety of rubber compositions, each designed to contribute some particular factor to overall performance. Rubber compounds designed for a specific function will usually be similar but not identical In composition and properties. Since 1970`s finite element analysis of tire has been performed extensively, which requires some energy density functions of rubber components of a tire. The conventional Mooney-Rivlin material model is one of the description that is commonly used in the analysis of tire. In this paper, we report the two material constants of gooney-Rivlin material model for some rubber compounds of a real pneumatic tire, which are obtained through uniaxial tension test.

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A FINITE-VISCOELASTIC CONTINUUM MODEL FOR RUBBER AND ITS FINITE ELEMENT ANALYSIS

  • Kim, Seung-Jo;Kim, Kyeong-Su;Cho, Jin-Yeon
    • Journal of Theoretical and Applied Mechanics
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    • v.1 no.1
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    • pp.97-109
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    • 1995
  • In this paper, a finite viscoelastic continuum model for rubber and its finite element analysis are presented. This finite viscoelatic model based on continuum mechanics is an extended model of Johnson and Wuigley's 1-D model. In this extended model, continuum based kinematic measures are rigorously defied and by using this kinematic measures, elastic stage law and flow rule are introduced. In kinematics, three configuration are introduced. In kinematics, three configuration are introduced. They are reference, current and virtual visco configurations. In elastic state law, it is assumed that at a certain time, there exists an elastic potential which describes the recoverable elastic energy. From this elastic potential, elastic state law is derived. The proposed flow rule is based on phenomenological observation. The flow rule gives precise relaxation response. In finite element approximation, mixed Lagrangian description is used, where total and similar method of updated Lagrangian descriptions are used together. This approach reduces the numerical job and gives simple nonlinear syatem of equations. To satisfy the incompressible condition, penalty-type modified Mooney-Rivlin energy function is adopted. By this method nearly incompressible condition is obtain the virtual visco configuration. For verification, uniaxial stretch tests are simulated for various stretch rates. The simulated results show good agreement with experiments. As a practical experiments. As a preactical example, pressurized rubber plate is simulated. The result shows finite viscoelastic effects clearly.

A Study on the Forming Characteristic of Inner Pyramid Structure Bonded Sheet Metal (피라미드형 내부구조재를 가지는 중공형 접합판재의 성형특성에 관한 연구)

  • Kim, J.Y.;Kil, H.Y.;Cho, G.C.;Kim, J.H.;Chung, W.J.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2006.05a
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    • pp.295-299
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    • 2006
  • The inner-structure bonded(ISB) sheet metal is defined as a composite sheet metal which has middle layer of truss-structure between two skin sheets. The characteristics such as ultra-light weight, high rigidity, high strength, etc are required especially for automobile parts. The characteristic of ISB sheet metal depends on inner-structure pattern or method of bonding. Pyramid type of crimped expanded metal is used for inner-structure and both of resistance welding and adhesive bonding are applied to make a specimen. As a result of compression test, it is appeared that forming limit is 10% reduction in thickness under a load of 8kgf per unit element(one inner-structure). In case of uniaxial tensile test the non-uniform surface integrity rather than the buckling of inner-structure happened at a load of 450kgf, which indicates elongation of 7.2% and thickness reduction of 13%. The eye-inspection method was applied to examine the defects occurring on the specimen during stretch forming. In case of biaxial stretch forming only the non-uniform deformation on the surface of a skin sheet could be observed. The forming limit in stretching of ISB sheet metal with the hemi-spherical punch of 150mm in diameter was 3mm in forming depth and 5% reduction in thickness.

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Constitutive equations for polymer mole and rubbers: Lessons from the $20^{th}$ century

  • Wagner, Manfred H.
    • Korea-Australia Rheology Journal
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    • v.11 no.4
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    • pp.293-304
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    • 1999
  • Refinements of classical theories for entangled or crosslinked polymeric systems have led to incommensurable models for rubber networks and polymer melts, contrary to experimental evidence, which suggests a great deal of similarity. Uniaxial elongation and compression data of linear and branched polymer melts as well as of crosslinked rubbers were analyzed with respect to their nonlinear strain measure. This was found to be the result of two contributions: (1) affine orientation of network strands, and (2) isotropic strand extension. Network strand extension is caused by an increasing restriction of lateral movement of polymer chains due to deformation, and is modelled by a molecular stress function which in the tube concept of Doi and Edwards is the inverse of the relative tube diameter. Up to moderate strains, $f^2$ is found to be linear in the average stretch for melts as well as for rubbers, which corresponds to a constant tube volume. At large strains, rubbers show maximum extensibility, while melts show maximum molecular tension. This maximum value of the molecular stress function governs the ultimate magnitude of the strain-hardening effect of linear and long-chain branched polymer melts in extensional flows.

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Some Remarks on the Experiment and Finite Element Analysis to Evaluate to Forming Limit of Sheet Metals (금속판재의 성형성 평가를 위한 실험 및 유한요소해석에 관한 고찰)

  • 곽인구;신용승;김형종;김헌영
    • Transactions of Materials Processing
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    • v.9 no.4
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    • pp.379-388
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    • 2000
  • This study aims to examine the influence of experimental and numerical factors on the results of the test and finite element simulation to evaluate the formability of sheet metals. The stretch-forming test with a hemispherical punch is carried out to obtain the limiting dome height (LDH) and forming limit diagram (FLD) for several kinds of aluminium and steel sheet. The results of the LDH and FLD tests are analysed to find any correlation with the uniaxial tensile properties. It proves that the size of the prescribed grid has great influence on the measured value of strain. The finite element analysis of the punch stretching process is also carried out and the result is compared with the experimental data. The influence of the numerical parameters such as friction coefficient, element size and anisotropy model on the simulation results tms out to be very considerable.

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Development of Hyperelastic Model for Butadiene Rubber Using a Neural Network

  • Pham, Truong Thang;Woo, Changsu;Choi, Sanghyun;Min, Juwon;Kim, Beomkeun
    • Elastomers and Composites
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    • v.56 no.2
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    • pp.79-84
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    • 2021
  • A strain energy density function is used to characterize the hyperelasticity of rubber-like materials. Conventional models, such as the Neo-Hookean, Mooney-Rivlin, and Ogden models, are widely used in automotive industries, in which the strain potential is derived from strain invariants or principal stretch ratios. A fitting procedure for experimental data is required to determine material constants for each model. However, due to the complexities of the mathematical expression, these models can only produce an accurate curve fitting in a specified strain range of the material. In this study, a hyperelastic model for Neodymium Butadiene rubber is developed by using the Artificial Neural Network. Comparing the analytical results to those obtained by conventional models revealed that the proposed model shows better agreement for both uniaxial and equibiaxial test data of the rubber.

A study on the development of high strength for acryl fiber during uniaxial stretching by swell-wet process (팽윤습열연신에 의한 아크릴섬유의 고강도화에 관한 연구)

  • Song, Kyoung-Hun;Lee, Mun-Soo
    • The Journal of Natural Sciences
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    • v.8 no.1
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    • pp.145-151
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    • 1995
  • The stretching of synthetic fibers by hot dry process is very difficult, because these fibers have high glass transition temperature at above $150^{\circ}C$. But, we used a swell-wet stretching precess; the fibers are stretched in a swelling agent such as organic solvents at lower temperature. In this study, 100% acryl fibers were uniaxially stretched with free width at $70^{\circ}C$ by swell-wet process in organic solvents. The stretchability was estimated by stretching work. This work is concerned with stretching stress and strain, and initial modulus. We found that it is a good parameter for the estimatation of high strength to the acrylic fiber. The effects of stretching conditions on the molecular orientation for high strength and mechanical properties of PAN fibers were measured.

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Tensile stress regulation of NGF and NT3 in human dermal fibroblast

  • Kim, Mi-Na;Hong, Jung-Woo;Nho, Min-Soo;Na, Yong-Joo;Shin, Jennifer Hyun-Jong
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1585-1587
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    • 2008
  • Fibroblast is constantly subjected to mechanical loads in connective tissues where mechanical signals are converted to intercellular biochemical events. The aim of this study is to understand the effects of tensile stress on the neurotrophin (NT) and transforming growth factor (TGF) expression of fibroblast in vitro. Nerve growth factor (NGF) stimulates fibroblast migration, and TGF is related to tissue repair. In this study, at the uniaxial stretch of 10% strain and frequency of 0.5 Hz, different resting times of 0, 20, and 60 min are placed in between 10 min stimulations periods. Results show increase in NGF mRNA levels and a substantial decrease in NT3 mRNA after 1 hr of stimulation, indicating that the tensile stress may regulate NGF and NT3, key factors for the neurocosmetic applications. The mRNA level for TGF-${\alpha}$ and TGF-${\beta}2$ had increased up to two-folds after 1 hr of stimulation, showing that the tensile stress may control TGF, an important part of wound healing.

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Circumferential Alignment of Vascular Smooth Muscle Cells in a Cylindrical Microchannel

  • Choi, Jong Seob;Piao, Yunxian;Kim, Kyung Hoon;Seo, Tae Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.274.1-274.1
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    • 2013
  • We report the circumferential alignment of human aortic smooth muscle cells (HASMCs) in an orthogonally micropatterned circular microfluidic channel to form an in vivo-like smooth muscle cell layer. To realize a biomimetic smooth muscle cell layer which is aligned perpendicular to the axis of blood vessel, we first fabricated a half-circular polydimethylsiloxane (PDMS) microchannel by soft lithography using a convex PDMS mold. The orthogonally micro wrinkle patterns were generated inside the half-circular microchannel by stretching-releasing operation under UV irradiation. Upon UV treatment with uniaxial 40 % stretch of a PDMS substrate and releasing process, the microwrinkle patterns perpendicular to the axial direction of the circular microchannel were generated, which could guide the circumferential alignment of HASMCs successfully during cultivation. The analysis of orientation angle, shape index, and contractile protein marker expression indicates that the cultured HASMCs revealed the in vivo-like cell phenotype. Finally, we produced circular microchannels by bonding two half-circular microchannels, and cultured the HASMCs circumferentially with high alignment and viability for 5 days. These results are the first demonstration for constructing an in vivo-like 3D smooth muscle cell layer in the circular microfluidic channel which can provide novel bioassay platforms for in-depth study of HASMC biology and vascular function.

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