• IMMUNE NETWORK
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• 제2권3호
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• pp.166-174
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• 2002

Background: Human umbilical cord bloods, which could be taken during the delivery are utilized as a source of hematopoietic stem cells. Also in cord blood, there are several kinds of stem cells such as endothelial and mesenchymal stem cells. Methods: We isolated the mesenchymal stem cells from human umbilical cord bloods and confirmed the differentiation of these cells into osteoblast progenitor cells. The mesenchymal stem cells derived from umbilical cord blood have the ability to differentiate into specific tissue cells, which is one of characteristics of stem cells. These cells were originated from the multipolar shaped cells out of adherent cells of the umbilical cord blood mononuclear cell culture. Results: The mesenchymal stem cells expressed cell surface antigen CD13, CD90, CD102, CD105, ${\alpha}$-smooth muscle actin and cytoplasmic antigen vimentine. Having cultrued these cells in bone formation media, we observed the formation of extracellular matrix and the expression of alkaline phosphatase and of mRNA of cbfa-1, ostoecalcin and type I collagen. Conclusion: From these results we concluded that the cells isolated from the umbilical cord blood were mesenchymal stem cells, which we could differentiate into osteoblast when cultured in bone formation media. In short, it is suggested that these cells could be used as a new source of stem cells, which has the probability to alternate the embryonic stem cells.

• Journal of Advanced Marine Engineering and Technology
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• 제33권7호
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• pp.981-986
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• 2009

The application of a cryogenic storage system is growing fast in different kinds of fields including to keep umbilical cord blood. Umbilical cord blood stem plays an important role in the treatment of a blood and immune system related genetic diseases, cancers and blood disorders. This study gives the optimal cryogenic system for cord blood banking. Three-dimensional models are employed and finite element method is used to do structure analyses of all designed models. The results shows model 3 have a good structure properties, and model 4 shows the best structure property as its maximum is 92.9 MPa. The other is too dangerous or infeasible to support load condition that allowed by STS 304. The results can be used in the design of these kinds of systems to obtain good predictions of trends over a wide range of design alternatives and operating conditions.

• Journal of Yeungnam Medical Science
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• 제8권1호
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• pp.98-106
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• 1991

본 연구에서 정상적인 분만 산모의 제대동맥혈의 pH와 신생아의 Apgar score및 질병의 이환유무를 관찰하였고, 또한 분만시 제대결찰 시간의 경과가 태아의 혈중가스농도의 변화 및 신생아의 건강에 미치는가를 분석해 보였다. 본 연구의 결과, 불량한 Apgar score를 보인 많은 신생아에서 산성증을 보인 경우가 적음으로 제대동맥혈의 pH와 Apgar score의 상관관계는 미약한 것으로 생각되며, 제대동맥혈의 분석이 Apgar score보다는 태아의 건강상태의 파악에는 좀더 도움이 되리라 생각한다. 그러나 제대동맥혈의 pH와 Apgar score등, 단독적인 지표만으로 초기 신생아의 건강 상태 및 평가 및 관리에 임상적 의의를 부여하기는 어려웠다. 또한 분만시 제대혈관의 결찰의 시간의 결정은 대부분의 산과의들의 토론의 대상처럼 어느 시간이 태아의 건강에 도움이 되는가는 결정할 수가 없었다. 그러나 신생아의 건강 상태를 파악하기 위해서 제대동맥혈을 채취할 경우는, 조기결찰의 특별한 적응증이 없는 한 분만직후에는 제대결찰을 시행하지 않고 즉시 제대동맥혈을 채취하는 것이 바람직하다고 할 수 있다. 이는 분만직후의 제대동맥혈이 분만진통이나 분만자체가 태아에 미치는 영향을 가장 잘 반영하기 때문으로 생각되어지기 때문이다. 태아의 건강의 지표로서 Apgar score, 제대혈관내의 pH와 가스농도의 분석이 그 정확도와 감수성을 더하기 위해서는 좀 더 많은 대상군과 좀 더 다양한 대조군으로 연구를 하여야 할 것 으로 사료된다.

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.187-195
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• 2007

The cord blood serves as a vehicle for the transportation of oxygen and nutrients to the fetus. In the past, the human cord blood has generally been discarded after birth. However, numerous studies have described the regenerative ability of the cord blood cells in various incurable diseases. The umbilical cord blood (UCB)-derived stem cells are obtained through non-invasive methods that are not harmful to both the mother and the fetus. Furthermore, the cord blood stem cells are more immature than the adult stem cells and expand readily in vitro. The mesenchymal stem cells (MSCs) have the capacity to differentiate in vitro into various mesodermal (bone, cartilage, tendon, muscle, and adipose), endodermal (hepatocyte), and ectodermal (neurons) tissues. This review describes the immunological properties of the human UCB-MSCs to assess their potential usefulness in the allogeneic transplantation for the regenerative medicine.

• 대한지역사회영양학회지
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• 제10권5호
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• pp.615-622
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• 2005

Folate and Vitamin $B_{12}$ are essential nutrients important during pregnancy. This study was conducted to evaluate the folate and vitamin $B_{12}$ nutritional status of Korean pregnant women and to investigate the relationship between maternal­umbilical cord serum folate and vitamin B12 levels and pregnancy outcomes. Dietary intakes of the pregnant women were estimated by 24 hour-recall (3 times). Serum folate and vitamin B12 levels in maternal blood and umbilical cord of 27 pregnant women at 1'st-, 2'nd-, 3'rd-trimester and delivery were measured by RIA (radioimmuno assay), respectively. Means of folate and vitamin $B_{12}$ intake were $283.53\pm58.01{\mu}g/day\;and\; 2.99\pm1.32mg/day$, respectively. Maternal mean serum folate levels of the trimester and delivery were $9.75\pm3.60ng/ml,\;10.46\pm4.63ng/ml,\;10.71\pm4.14ng/ml\;and\;15.05\pm7.04ng/ml$. Those maternal levels were significantly lower than that of umbilical cord blood $(23.99\pm9.42ng/ml)$. Serum vitamin $B_{12}$ levels of maternal trimester and delivery were $479.07\pm137.56 pg/ml,\;310.96\pm137.56pg/ml,\;308.22\pm74.65pg/ml,\;and\;295.67\pm93.36pg/ml$, which were significantly lower than those of umbilical cord blood $(500.13\pm185.60ng/ml)$. This finding indicates that the uptake of folate and vitamin $B_{12}$ in the fetus may be due to an active placental transport mechanism. Maternal serum level correlated positively with those of umbilical cord blood, showing that folate and vitamin $B_{12}$ concentration of umbilical cord blood might be affected by maternal status. There was no significant correlation between the serum folate levels in maternal-umbilical cord blood and the pregnancy outcomes. However, maternal vitamin $B_{12}$ level at l'st trimester was significant positive correlation between the gestational age except for birth weight and weight gain.

• 대한지역사회영양학회지
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• 제10권6호
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• pp.860-868
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• 2005

Anemia diagnosed early in pregnancy is associated with increased risks of low birth weight and preform delivery. The purposes of this study were to assess the maternal iron status during pregnancy and to evaluate the relationships between the iron indices of maternal-umbilical cord serum iron and ferritin levels and pregnancy outcomes. Dietary intakes of the pregnant women were estimated by 24 hour-recall (3 times). Serum iron and ferritin levels in maternal blood and umbilical cord were measured at 1st-, 2nd-, 3rd- trimester and delivery, respectively. The mean of maternal se겨m iron levels of the trimester and delivery were $124.27\;{\mu}g/dl,\;97.03\;{\mu}g/dl,\;94.32\;{\mu}g/dl,\;and\;145.53\;{\mu}g/dl$. Those maternal levels were significantly lower than that of umbilical cord blood ($222.59\;{\mu}g/dl$). Serum ferritin levels of maternal trimester and delivery were 22.68 $22.68\;{\mu}g/l,\;11.09\;{\mu}g/l,\;14.18\;{\mu}g/l,\;and\;\;24.54\;{\mu}g/l$, which were significantly lower than those of umbilical cord blood ($184.35\;{\mu}g/l$) (p < 0.0001). This prevalence of anemia of total subjects was $30.3\%$ by WHO criteria (Hb < 11.0 g/dl, Hct < $33\%$). Iron levels of 2nd-trimester was significantly higher in the normal group than in the anemia group. And ferritin levels of 3rd-trimester and delivery was significantly higher in the normal group than in the anemia group. Therefore, we suggest for successful pregnancy outcome and delivery differential iron supplementation programs will be carried out with individual Pregnant women on the basis of pre-Pregnancy nutrition. (Korean J Community Nutrition 10(6) : $860\∼868$, 2005)

• 한국가축번식학회지
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• 제27권2호
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• pp.97-102
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• 2003

본 연구는 분만시 동복 신생자돈의 제대혈에서 추출한 genomic DNA를 PCR-RFLP 기법을 이용하여 농가수입증대를 위하여 육질이 불량한 PSS 돼지를 판별하는 방법을 개발하기 위한 기초실험으로써 그 결과를 요약하면 다음과 같다. 자돈 제대에서 혈액 genomic DNA를 추출하여 PCR에 의하여 증폭된 ryanodine receptor gene 영역의 산물은 자돈의 제대혈에서 1.8kb의 길이로 증폭되었음을 확인하였다. 제대혈에서 추출된 DNA 의 PCR 증폭 단편을 가지고 Hha I 제한효소로 digest 하여준 결과에서 PSS 돼지는 Yorkshire 종에서 출현하지 않았으나, Landrace 종과 Crossbred 종에서 각각 4.76%와 7.14%로 교잡종(LYD 또는 YLD)에서 더욱 많이 출현되었다. 이상의 결과로 볼 때 분만시 신생자돈의 제대혈을 채취하여 PSS 돼지를 조기에 선발하면 스트레스 감소는 물론 혈액채취의 간편성을 기대할 수 있을 것으로 판단된다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2005년도 제60회 한국생물과학협회 정기학술발표대회
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• pp.26-26
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• 2005

• 한국식품위생안전성학회지
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• 제27권1호
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• pp.74-80
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• 2012

어류는 두뇌발달에 중요한 영양소이기도 하지만 신경독인 메틸수은을 함유하고 있는 것으로 알려져 있다. 메틸수은은 임산부가 임신 중에 태아의 두뇌에 치명적인 손상을 유발하는 것으로 알려져 있다. 따라서 연구자들은 생선소비와 임산부 제대혈 중의 메틸수은 함량을 확인하기 위하여 통영지역 임산부 159명을 대상으로 2010년 10월부터 2011년 3월 사이에 임산부의 생선소비와 제대혈의 수은농도의 관계를 조사하였다. 어류소비 관련자료는 설문조사를 통하여 수집하였고 통계처리는 one way ANOVA 를 이용하였다. 조사에 참여한 임산부의 평균나이는 30.1세이었고 임산부 제대혈의 수은 함량은 0.01-14.80 ppb 범위에 평균 $2.69{\pm}2.50ppb$이었다. 제대혈의 평균 수은함량은 WHO 기준인 5.0 ppb를 초과하지는 않았지만 159명 중 17명(10.7%)이 WHO 기준을 초과하였다. 연령별로는 30세 이상이 0.01-14.8 ppb 범위, 평균 $3.18{\pm}2.74ppb$로 29세 이하에서 0-8.00 ppb 범위, 평균 $2.04{\pm}2.00ppb$ 보다 1.6배 높았으며 유의적 차이가 있었다. 이 연구에서 생선섭취가 제대혈의 수은농도에 영향을 미치는 것으로 나타났다. 1주일에 3회 이상 섭취군이 평균 $4.15{\pm}4.02ppb$로 주 1-2회 섭취군의 $2.63{\pm}2.22ppb$, 먹지 않는 군의 $1.60{\pm}1.44ppb$ 보다 2.6배와 1.6배 각각 높게 나타났으며 적게 먹는 군과 먹지 않는 군과의 사이에는 유의적 차이가 없었고, 많이 섭취하는 군과는 유의적 차이 (p < 0.05)가 있었다. 이상의 결과에서 제대혈의 수은 농도는 생선섭취량과 관련이 있는 것으로 판단되며 가임기 여성이나 임산부에게 생선섭취를 제한하는 것이 제대혈의 수은 농도를 낮출 수 있고 향후 있을지도 모를 신생아의 신경발달 장애나 뇌 신경학적 손상을 줄일 수 있는 방법이라고 생각되었다.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2009년도 추계학술발표대회 논문집
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• pp.409-414
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• 2009

Since cryogens are stored at very low temperatures, the cryogenic storage systems are quite sensitive to heat leaks. Even though the vessel operated under sealed condition with vacuum insulation and reflective coatings are used, the heat leakage into the vessel is still unavoidable. Therefore, this paper concerns with numerical study of self-pressurization used to analysis the optimum design with the variation volume fraction, effect of heat flux and storage pressure of liquid nitrogen. The result shows that as the volume fraction increases, the pressure rise reduces and the relatively at atmosphere pressure is better than the higher one. In addition, higher heat flux leads the pressure rise increases faster than low one. The additional of heat pipe system to reduce the pressure rise rate also has been done. By this comparison, the optimum design for storage umbilical cord blood can be selected.