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Detection of PSS Gene through Genomic DNA of Umbilical Cord Blood by PCR-RFLP in Piglets  

김계웅 (공주대학교 산업과학대학 동물자원학과)
유재영 (건국대학교 축산대학 축산학과)
박홍양 (건국대학교 축산대학 축산학과)
윤종만 (군산대학교 해양과학대학 해양생명과학부)
조규석 (공주대학교 산업과학대학 동물자원학과)
정재록 (공주대학교 산업과학대학 동물자원학과)
김건중 (공주대학교 산업과학대학 동물자원학과)
이종완 (공주대학교 산업과학대학 동물자원학과)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.2, 2003 , pp. 97-102 More about this Journal
Abstract
This study was carried out to find out PSS(Porcine Stress Syndrome) with the PSE(Pale, Soft, Exudative) in different piglets. These experiments were accomplished with the aid of PCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The samples were collected and examined from umbilical cord blood of piglets of Yorkshire, Landrace and Crossbred. And then, the PCR products were digested by restriction enzyme, Hha I. The results obtained were as follows; The PCR products of the blood genomic DNA of ryanodine receptor gene were length of 1 .8kb in umbilical cord blood. Normal type(NN), heterozygous type(Nn) and recessively homozygous type(nn, PSS) as a result of digestion of restriction enzyme, Hha 1, were 90.0%, 10.0% and 0.0% in Yorkshire piglets, 76.2%, 19.0% and 4.8% in Landrace, 69.1%, 23.8% and 7.1% in crossbred, respectively. As already showing the above results, the blood from piglets umbilical cord can be availably used for the determination of genotypes of PSS because of easiness of blood collection without stress in live piglets.
Keywords
PSS; Umbilical cord blood; PCR-RFLP; Restriction enzyme;
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