• 한국환경농학회지
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• 제23권4호
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• pp.251-257
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• 2004

Analytical methods were developed to determine cyclosulfamuron residues in soil, water, rice grain and straw using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. In these methods, cyclosulfamuron was extracted with aqueous $Na_2HPO_4$/acetone and acetone/methanol mixture from soil and rice samples respectively. Liquid-liquid partition coupled with ion-associated technique, Florisil column chromatography, and solid-phase extraction (SPE) were used to separate cyclosulfamuron from interfering co-extractives prior to HPLC analysis. For water sample, the residue was enriched in $C_{18}$-SPE cartridge, cleaned up in situ, and directly subjected to HPLC. Reverse-phase HPLC under ion-suppression was successfully applied to determine cyclo-sulfamuron in sample extracts with the detection at its ${\lambda}_{max}$ (254 nm). Recoveries from fortified samples averaged $87.8{\pm}7.1%$ (n=12), $97.3{\pm}7.2%$ (n=12), $90.8{\pm}6.6%$ (n=6), and $78.5{\pm}6.7%$ (n=6) for soil, water, rice grain and straw, respectively. Detection limits of the methods were 0.004 mg/kg, 0.001 mg/L, 0.01 mg/kg and 0.02 mg/kg for soil, water, rice grain and straw samples, respectively.

• 한국축산식품학회지
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• 제41권2호
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• pp.335-342
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• 2021

Vitamin B12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC) was developed for the rapid detection and quantitation of vitamin B12 in powdered milk. Detection limit and powdered milk recovery were determined by quantitative analysis. The limits of detection and quantitation were 2.71 and 8.21 ㎍/L, respectively. Relative standard deviations of the intra-day and inter-day precisions varied in the ranges of 0.98%-5.31% and 2.16%-3.90%, respectively. Recovery of the analysis varied in the range of 83.41%-106.57%, suggesting that the values were acceptable. Additionally, vitamin B12 content and recovery in SRM 1849a were 54.10 ㎍/kg and 112.24%, respectively. Our results suggested that the analytical method, including the sample pretreatment step, was valid. This analytical method can be implemented in many laboratory-scale experiments that seek to save time and labor. Therefore, this study shows that immunoaffinity-HPLC/ultraviolet is an acceptable technique for constructing a reliable database on vitamin B12 in powdered milk containing starch as well as protein and/or fat in high amounts.

• 한국군사과학기술학회지
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• 제27권2호
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• pp.213-221
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• 2024

Aircraft's operational effectiveness is reduced due to threats from enemy anti-aircraft weapons, which is a weak point. In particular, guided missiles, which pose a threat to aircraft, are rapidly developing due to technological advancements in seekers, and are classified as one of the important technologies in weapon systems. Missile approach warning equipment installed to ensure aircraft survivability detects guided missiles and provides relevant information to respond. Tests were conducted domestically to verify the detection level of missile approach warning equipment, and test results were presented under various test conditions.

• 대한약침학회지
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• 제17권4호
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• pp.36-49
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• 2014

Objectives: Quercetin and curcuminoids are important bioactive compounds found in many herbs. Previously reported high performance liquid chromatography ultraviolet (HPLC-UV) methods for the detection of quercetin and curcuminoids have several disadvantages, including unsatisfactory separation times and lack of validation according the standard guidelines of the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. Methods: A rapid, specific, reversed phase, HPLC-UV method with an isocratic elution of acetonitrile and 2% v/v acetic acid (40% : 60% v/v) (pH 2.6) at a flow rate of 1.3 mL/minutes, a column temperature of $35^{\circ}C$, and ultraviolet (UV) detection at 370 nm was developed. The method was validated and applied to the quantification of different types of market available Chinese medicine extracts, pills and tablets. Results: The method allowed simultaneous determination of quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin in the concentration ranges of $0.00488-200{\mu}g/mL$, $0.625-320{\mu}g/mL$, $0.07813-320{\mu}g/mL$ and $0.03906-320{\mu}g/mL$, respectively. The limits of detection and quantification, respectively, were 0.00488 and $0.03906{\mu}g/mL$ for quercetin, 0.62500 and $2.50000{\mu}g/mL$ for bisdemethoxycurcumin, 0.07813 and $0.31250{\mu}g/mL$ for demethoxycurcumin, and 0.03906 and $0.07813{\mu}g/mL$ for curcumin. The percent relative intra day standard deviation (% RSD) values were $0.432-0.806{\mu}g/mL$, $0.576-0.723{\mu}g/mL$, $0.635-0.752{\mu}g/mL$ and $0.655-0.732{\mu}g/mL$ for quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin, respectively, and those for intra day precision were $0.323-0.968{\mu}g/mL$, $0.805-0.854{\mu}g/mL$, $0.078-0.844{\mu}g/mL$ and $0.275-0.829{\mu}g/mL$, respectively. The intra day accuracies were 99.589%-100.821%, 98.588%-101.084%, 9.289%-100.88%, and 98.292%-101.022% for quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin, respectively, and the inter day accuracy were 99.665%-103.06%, 97.669%-103.513%, 99.569%-103.617%, and 97.929%-103.606%, respectively. Conclusion: The method was found to be simple, accurate and precise and is recommended for routine quality control analysis of commercial Chinese medicine products containing the flour flavonoids as their principle components in the extracts.

• 한국식품위생안전성학회지
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• 제22권4호
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• pp.370-374
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• 2007

본 논문에서는 테트라사이클린(TCs)계 항생물질 4종(옥시테트라사이클린, 테트라사이클린, 클로르테트라사이클린, 독시사이클린)에 대하여, 액-액 추출 과정을 거쳐서 자외선검출기가 장착된 액체크로마토그래피를 이용하여 계란 중에서 TCs를 효율적으로 분석하는 방법을 확립하였다. 컬럼은 Zorbax Eclipse XDB-C8$(150mm{\times}4.6mm,\;5{\mu}m)$, 이동상 용매는 20 mM oxalic acid(pH 1.5), acetonitrile로 기울기 용리를 사용하였으며, 검출파장은 360 nm로, 그리고 유속은 1.0 ml/min, 주입량은 10 ml로 설정하여 분석하였다. 확립된 분석조건으로, TCs 4종에 대한 표준검정 곡선은 $50-800{\mu}g/kg$의 농도범위에서 상관계수가 0.994이상의 양호한 직선성을 나타내었다. 회수율은 $50-800{\mu}g/kg$의 농도범위에서 79.6-109.3%로, 향상된 추출효율을 나타내었으며, 검출한계는 $30{\mu}g/kg$이었고, 정량한계는 $50{\mu}g/kg$으로서 MRL/4 수준 이하까지는 충분히 검출 가능하였다. 또한, 일내(intra-day)와 일간(inter-day) 정밀도(RSD)는 0.1-10.9%, 1.1-13.7%이었다. 따라서, 확립된 분석방법은 계란 중의 TCs을 효과적으로 분석하는데 이용될 수 있을 것으로 사료된다.

• 융합신호처리학회논문지
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• 제23권4호
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• pp.256-263
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• 2022

피부암은 세계에서 가장 흔한 질병 중 하나로 국내에선 발병률이 지난 5년 동안 약 100%가 증가했고 미국에선 매년 500만여 명이 피부암을 진단받는다. 피부암은 주로 자외선의 노출로 피부 조직이 오랜 시간 손상되면서 발생하게 된다. 피부암의 악성종양인 흑색종은 피부 위에서 발생하는 멜라닌 세포 모반과 생김새가 유사해 2차 징후가 발생하지 않는 한 일반인이 자각하기 어려운 점이 있다. 본 논문에서는 이러한 피부암의 조기 발견과 분류를 위해 피부암 병변 윤곽선 검출 알고리즘과 피부암 병변 분류를 수행하는 딥러닝 모델인 CRNN을 제안한다. 실험 결과 본 논문에서 제안하는 윤곽선 검출 알고리즘을 이용할 시 분류 정확도가 97%로 가장 높은 정확도를 보였고 Canny 알고리즘의 경우 78%를 보였고 Sobel의 경우 55%, Laplacian의 경우 46%를 보였다.

• 분석과학
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• 제27권3호
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• pp.147-152
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• 2014

범죄 현장에서 눈으로 보이지 않는 지문의 위치를 파악하기 위해 254 nm의 단파자외선과 루비스(RUVIS;Reflective Ultraviolet Imaging System, 반사자외선이미징시스템) 장비를 사용하는 것이 매우 효과적이다. 최근 유전자 감식 기술의 발전으로 지문과 같은 극미량의 생체시료에서도 성공적으로 DNA 프로필을 확보할 수 있게 되었지만, 지문 탐색에 사용되는 단파자외선에 의해 DNA가 파괴될 수 있다. 본 연구에서는 일반적으로 가장 많이 사용되고 있는 4 종류의 자외선 광원을 대상으로 자외선 조사 시간과 조사 거리에 따른 DNA 손상 정도를 비교하였다. 단파 자외선을 사용하는 경찰 루비스, SIRCHIE 미니라이트 및 SIRCHIE 루비스의 경우에는 10 cm 거리에서 10초간 조사할 경우 약 50% 정도의 DNA가 손상되었고, 시료와의 거리가 가까울수록, 처리 시간이 길수록 DNA 손상 정도가 증가하였다. 이 장비들을 사건 현장에서 사용할 경우에는 유전자 감식 시료의 DNA에 많은 손상을 가져올 수 있기 때문에 1 m 이상의 거리에서 조사하는 것이 바람직할 것으로 판단되었다. 반면 350 nm의 장파자외선을 사용하는 폴리라이트 장비는 단파 자외선 장비에 비해 DNA 손상 정도가 크지 않았다. 지문 탐색과 유전자감식을 모두 고려한다면, 자외선 광원의 종류에 따라 조사 거리와 조사 시간을 결정하는 것이 필요하다.

• Journal of Acupuncture Research
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• 제32권2호
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• pp.1-9
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• 2015

Objectives : The present study was an evaluation and standardization of herbal components in order to establish the efficacy and safety of Shinbaro pharmacopuncture. Methods : Among the raw materials of Shinbaro pharmacopuncture, the components Cibotii Rhizoma, Eucommiae Cortex, and Ledebouriellae Radix were assessed through ingredient verification experiments using thin-layer chromatography(TLC) and ultraviolet rays(UV) lamps. In addition, we standardized Acanthopanacis Cortex and Achyranthis Radix through validation using high performance liquid chromatograph-diode array detector(HPLC-DAD). Results : As result appeared a blue-white fluorescence under ultraviolet rays; changed to dark green after adding 1 % ferric chloride solution(due to Cibotii Rhizoma), and presented a yellow-green fluorescence when mixed with an ethyl ether under UV lamps by way of the ethyl ether layer, confirming Eucommiae Cortex. Ledebouriellae Radix was confirmed as dark brown spots at Rf values of 0.56 and 0.71 using TLC. Additionally, Acanthopanacis Cortex and Achyranthis Radix HPLC test results showed that linearity was $R^2{\geq}0.99$, and detection limit and quantitation limit were 0.23 to $1.29{\mu}g/mL$, and 0.71 to $3.90{\mu}g/mL$, respectively. Furthermore, precision and accuracy were confirmed to have relative standard deviation(RSD) values of 0.10 to 1.89 % and 96.19 to 103.72 %, respectively. Shinbaro pharmacopuncture did not have any overlapping or interference from other peaks in detection under the abovementioned analysis conditions. Conclusions : In conclusion, we confirmed that maintenance of Shinbaro pharmacopuncture validity was possible by means of quality control of Cibotii Rhizoma, Eucommiae Cortex, and Ledebouriellae Radix through ingredient identification and Acanthopanacis Cortex and Achyranthis Radix through high performance liquid chromatograph(HPLC) analysis. Further, we hope to contribute to the development strategy of herbal industry acupuncture.

• Fisheries and Aquatic Sciences
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• 제7권3호
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• pp.148-162
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• 2004

The monitoring activities at the National Fisheries Research and Development Institute (NFRDI) in Korea have been extended to include all the coastal waters of Korea after the outbreak of Cochlodinium polykrikoides blooms in 1995. We used several alternative methods including climatological analysis, spectral and optical methods which may offer potential detection of the major species of red tide in Korean waters. In the climatological analysis, NOAA, SeaWiFS, OCM satellite data was chosen using the known C. polykrikoides red tide bloom data and the area was mapped by helicopter reconnaissance and ground observation. The relationship between the distribution of sea surface temperature to C. polykrikoides bloom areas was studied. The anomalies of SeaWiFS chlorophyll a imageries against the imageries of non-occurring red tide for August, 2001 showed where the C. polykrikoides occurred. The anomalies of chlorophyll a concentrations from the satellite data during red tide outbreaks showed a similar distribution of C. polykrikoides in the red tide in August, 2001. The distribution between differences in sea surface temperatures during the day and at night also showed a possibility for red tide detection. We used a corrected vegetation index (CVI) to detect floating vegetation and submerged vegetation containing algal blooms. The results of from the optical absorption of C. polykrikoides in the ultraviolet band (340 nm) showed that if we use the optical characteristics from each red tide, we will be able to establish the feasibility of red tide detection.

• Applied Biological Chemistry
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• 제41권8호
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• pp.595-599
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• 1998

An analytical method was developed to determine tricyclazole residues in rice grain, straw, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Tricyclazole was extracted with methanol from moist rice grain, straw, and soil samples. n-Hexane washing was employed to remove nonpolar co-extractives during liquid-liquid partition. Tricyclazole was then extracted with dichloromethane from alkaline aqueous phase, while acidic interferences remained in the phase. Dichloromethane extract was further purified by silica gel column chromatography prior to HPLC determination. Reverse-phase HPLC using an octadecylsilyl column was successfully applied to separate and quantitate the tricyclazole residue in sample extracts monitored at ${\lambda}_{max}$ 225nm. Recoveries from fortified samples averaged $95.5{\pm}3.0%\;(n=6),\;87.5{\pm}20.%\;(n=6),\;and\;84.3{\pm}2.8%$ (n=12) for rice grain, straw, and soil, respectively. Detection limit of the method was 0.02 mg/kg for rice grain and soil samples while 0.05 mg/kg for rice straw samples. The proposed method was reproducible and sensitive enough to evaluate the safety of tricyclazole residues in rice grain, straw, and soil.