• Journal of Biosystems Engineering
• /
• 제31권6호
• /
• pp.506-513
• /
• 2006

The effects of ultrasound stimuli on the germination and sprout growth of brown rice were investigated. Ultrasound was applied to brown rice at the frequencies of 28, 40, and 60 kHz before germination test and it was germinated in three methods (Type I, II and III). Type I was to soak brown rice into water for 60 hours. Type II was to expose brown rice to air for 48 hours after soaking them into water for 12 hours. Type III was a repetitive method of water-soaking and air exposure for 12 hours respectively. The most effective method for the germination was Type III without ultrasound. However, Type I was a best method after ultrasound treatment. As power of ultrasound increased, sprouts grew faster after brown rice were treated in 40%, 70%, and 100% power (0.137, 0.241, and 0.344 $W/cm^2$) at 40 kHz. The good treatments for fast sprout growth of brown rice at each frequency were the 28 kHz-10min group, the 40 kHz-5min group, and the 60 kHz-20min group of Type I. The best effective treatment was the 40 kHz-5min group at 0.344 $W/cm^2$ and at that condition the time required for sprout growth of 2.5 m was 51.9 hours. The ultrasound stimuli was very effective in the beginning of the rice germination, and the germination ratio was more than 95% in all ultrasound treatments.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1700-1703
• /
• 2008

The purpose of this study was to examine the effects of various mechanical stimuli for MC3T3-E1 cells. Among the several mechanical stimulations, we focused on compressive stain and ultrasound. In this study, we developed a bioreactor capable of applying controlled stimuli to scaffolds. PLLA/PCL scaffold was fabricated by using salt-leaching method. We performed dynamic cell culture using preosteoblasts MC3T3-E1 cells with 1MHz, 30mW/cm2 ultrasound and 10% of compressive strain. Result of CCK-8 analysis at 1, 4, 7, 10 days showed that mechanical stimuli had no significant effect for cell proliferation. However, those stimuli influenced ALP(Alkaline phopatase) activity, which is one of differentiation marker.

• BMB Reports
• /
• 제56권3호
• /
• pp.145-152
• /
• 2023

Mechanosensitive ion channels sense mechanical stimuli applied directly to the cellular membranes or indirectly through their tethered components, provoking cellular mechanoresponses. Among others, Piezo1 mechanosensitive ion channel is a relatively novel Ca²⁺-permeable channel that is primarily present in non-sensory tissues. Recent studies have demonstrated that Piezo1 plays an important role in Ca²⁺-dependent cell death, including apoptosis and ferroptosis, in the presence of mechanical stimuli. It has also been proven that cancer cells are sensitive to mechanical stresses due to higher expression levels of Piezo1 compared to normal cells. In this review, we discuss Piezo1-mediated cell death mechanisms and therapeutic strategies to inhibit or induce cell death by modulating the activity of Piezo1 with pharmacological drugs or mechanical perturbations induced by stretch and ultrasound.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2021년도 추계학술대회
• /
• pp.397-399
• /
• 2021

본 논문에서는 폐암 세포 증식을 억제하기 위해 멀티모달 시스템 개발에 관한 기본연구를 수행하였다. 광 자극 시스템과 특정한 주파수를 발생시키는 초음파를 이용하여 폐암 세포를 대상으로 자극을 인가하였고, 세포의 증식 억제 효과를 영상화하여 정량적으로 평가하였다. 실험 결과, 초음파와 LED 단일 자극을 인가하였을 때와 동시에 인가하였을 때 폐암 세포의 증식률에서 의미 있는 결과를 보였다.

• The Journal of Korean Physical Therapy
• /
• 제17권4호
• /
• pp.589-600
• /
• 2005

The purposes of this study is to discuss and analyze the effect on the recovery from cut in sciatic nerve. This study used 9 weeks male rats of Sprague-Dawley family. Rat in groups 4 were treated with pulsed therapeutic ultrasound for 3 minutes. 3 times weekly at 3MHz respectively (intensity; $0.2W/cm^2,\;0.5W/Cm^2,\;10W/cm^2$); rat in group 1 received placebo ultrasound. In addition, changes of serum aspartate amino-transferase(AST) and creatine phosphokinase(CPK) levels were also demonstrated with diameter of individual muscle fasciculate and number of muscle fiber in each of three types of muscles located in gastrocnemius, soles. The results of comparing the changes in groups are as follows; 1. We found out that hypertrophic epineurium was present in sciatic nerve injured ultrasound treatment of groups. 2. In the gastrocnemius morphological investigation of the group I (control group), severe muscle atrophy were observed at the 7th days of the sciatic nerve injury. however, muscle atrophy of the group IV ($1.0W/cm^2$) were slightly recovered at the 14th days after treatment ultrasound. At the 28th days, muscular fibers were formed in polygon and were significantly recovered. 3. C-fos immunoreactive of the group II ($0.2W/cm^2$), III ($0.5W/cm^2$) were remarkably increased at the 1th day after treatment of ultrasound. Group IV were markedly deceased. 4. Brain-Derived Neurotrophic Factor(BDNF) immunoreactive of the group II, III were increased after 7 days of the sciatic nerve injury. Group IV were markedly increased from 14th days to 28th days after treatment of ultrasound. 5. A significant increase of serum AST levels were demonstrated in control group. However, serum AST levels of massage groups were significantly decreased compared to that of control group in followed order ; ($0.2W/cm^2<0.5W/cm^2<1.0W/cm^2$). 6. A significant increase of serum CK levels were demonstrated in control of group. However, serum CK levels of massage groups were significantly decreased compared to that of control group in followed order ; ($0.2W/cm^2<0.5W/cm^2<1.0W/cm^2$). The above results suggest that ultrasound treatment after peripheral nerve injury might reduce noxious stimuli, facilitate nerve recovery and effective in the functional improvement delaying muscle atrophy or degeneration.

• 생태와환경
• /
• 제40권2호
• /
• pp.244-253
• /
• 2007

본 연구에서는 잉어의 생태 반응에 미치는 초음파(강도 31 dB re 1 ${\mu}Pa$, 주파수 $14{\sim}15$ kHz)의 영향을 구명하기 위하여 치사율, 체중변화, 건강성 평가 및 행동패턴을 관찰하였다. 실험기간 동안 초음파에 노출된 잉어 Cyprinus carpio의 치사율은 대조군을 포함하여 처리군 모두 5% 이하로 실험구 사이의 치사율은 뚜렷하게 구별되지 않았다(P>0.05). 각각의 실험구에서 측정한 실험어류의 평균 체중은 실험종료 시 대조군에 비해 처리군에서 약간 증가하는 경향을 보였으나 실험구간의 뚜렷한 차이는 보이지 않았다. 대조군과 고처리군의 일부 개체들은 가벼운 표피손상과 꼬리지느러미의 출혈 흔적이 관찰되었으나 눈, 지느러미, 기생충 감염, 흉선의 출혈 및 아가미에서는 이상증상이 전혀 관찰되지 않았다(P>0.05). 근거리에서 일시적으로 초음파를 발생시켰을 때 어류는 음원으로부터 회피하는 방향성 움직임을 보였으나 원거리에서 초음파에 노출된 잉어는 뚜렷한 행동변화를 보이지 않았다. 결론적으로 낮은 강도의 초음파에 노출된 어류는 초음파 발생에 따른 심각한 스트레스는 받지 않을 것으로 판단된다.

• Journal of Acupuncture Research
• /
• 제23권3호
• /
• pp.231-239
• /
• 2006

Functional medicine is a system which utilizes certain investigative and treatment methods that are primarily oriented toward the recognition of functional disorder. Functional disorders take place on an energetic plane. This plane is not capturable using our regular investigatory techniques, such as X -ray. ultrasound, computer tomography and laboratory analyses. The 7-zone-diagnostic system(VEGA-DFM 722 and ABR-2000, etc) is a diagnostic device Which applies pulse signals to predetermined bodily locations. Applying alternating positive and negative stress to tissues with positive and negative pulses first manifests itself as negative and positive wave forms. The skin has many functions as an organ. It is the organism's interlace with the outsider world. Hence, the manner in which the skin reacts to external stimuli reflects the current state of the organism(as meridian systems). This system make a diagnosis of functional disorder or some typical organic diseases include stress of the mind or the body, and the energetic situation, reserves. We are known a disorder or disease throw the result chart. This result chart contain disorder flow wave forms and some valuable diagnostic hints (letters ; S, OM, RA, etc). In spite of this 7-zone-diagnostic system that is applied plentifully in the clinical application, there is not a statistics which is suitable. Therefor we must carry out many various research in the future.

• The Journal of Korean Physical Therapy
• /
• 제12권1호
• /
• pp.49-56
• /
• 2000

The purpose of this study was to investigate the influnce of afferent stimuli, transcutaneous electrical nerve stimulation and ultra sound, on the electrdiagnostic study of normal subjects. Electrodiagnostic study was performed before and after the application of afferent stimulation of the right popliteal fossa on 18 healthy female volunteers. After the transcutaneous electrical nerve stimulation, there is no significantly change of latencies and amplitudes of SEP, H-reflex, peroneal nerve F-wave, and sensory nerve conduction. After the ultra sound, there is no significantly change of latencies and amplitudes of SEP, H-reflex, peroneal nerve F-wave, and sensory nerve conduction. Tibial nope F-wave and motor nerve shows prolonged latency after TENS and US (p<0.01). Ultrasound may have a similar mechanism of action compared to transcutaneous electrical nerve stimulation by having localized inhibitory effects of the peripheral nerve. However, further investigation is needed to assess their mechanism of action and the precise relevance of stimulation modality.