• Title/Summary/Keyword: UVS

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Analysis of epistatic interactions and properties of UV-sensitive, uvs mutants of Aspergillus nidulans (Aspergillus nidulans의 자외선 감수성, uvs 돌연변이주들의 epistatic 연관성 및 성질에 대하여)

  • Chae, Suhn-Kee
    • The Journal of Natural Sciences
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    • v.11 no.1
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    • pp.45-54
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    • 1999
  • In epistatic grouping of uvs genes in A. nidulans based on the sensitivities to 4-NQO, the same epistatic grouping was obtained as those for UV and MMS-sensitivities. Based on the MMS-sensitivities, uvsA demonstrated synergistic interactions to uvsF and uvsH, the UvsF group genes, but exhibited epistatic interactions to uvsB and uvsC. The same epistatic grouping was also seen for uvsI when UV was irradiated after 4h germination of conidia, showing synergistic interactions to uvsH, uvsC, and uvsB. However, epistatic interactions were observed with uvsF, which were different from those obtained in quiescent conidia by UV. Intergenic and intragenic recombination frequencies were normal in uvsI compared with wild type.

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Different properties of mutagen sensitive musN mutant, a member of the UvsC group, from uvsC mutant strains in Aspergillus (Aspergillus의 UvsC group에 속한 돌연변이원 감수성 변이주 musN이 uvsC 돌연변이주와 다른 성질)

  • Chae, Suhn-Kee
    • The Journal of Natural Sciences
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    • v.8 no.2
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    • pp.43-48
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    • 1996
  • Mutangen sensitive hyper-rec type musN mutants were assigned into the UvsC group which contains genes involved in recombination and mutation. however, phenotypic properties of musN mutants were very different from those found in uvsC mutant strains which are rec- and lack UV-induced mutation. musN was not a mutator like uvsC. In addition, selenate resistant mutations in musN were induced sililar to those in wild types by UV irradiation. Wild type levels of UV-sensitivity in dividing cells of musN also differ from the uvsC phenotypes. These indicate that the UvsC group has branched pathways.

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A Novel UV-Sensitivity Mutation Induces Nucleotide Excision Repair Phenotype and Shows Epistatic Relationships with UvsF and UvsB Groups in Aspergillus nidulans

  • Baptista, F.;Castro-Prado, M.A.A.
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.102-108
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    • 2001
  • DNA damage response has a central role in the maintenance of genomic integrity while mutations in related genes may result in a range of disorders including neoplasic formations. The uvsZl characterized in this report is a navel uvs mutation in Aspergillus nidulans, resulting in a nucleotide excision repair (NER) phenotype: UV-sensitivity before DNA synthesis (quiescent cells), high UV-induced mutation frequency and probable absence of involvement with mitotic and meiotic recombinations. The mutation is recessive and nan-allelic to the previously characterized uvsA101 mutation, also located on the paba-y interval on chromosome I. uvsZl skewed wild-type sensitivity to MMS, which suggests non-involvement of this mutation with BER. Epitasis tests showed that the uvsZ gene product is probably involved in the same repair pathways as UVSB or UVSH proteins. Although mutations in these proteins result in an NER phenotype, UVSB is related with cell cycle control and UVSH is associated with the post-replicational repair pathway. The epistatic interaction among uvsZl and uvsB413 and uvsH77 mutations indicates that different repair systems may be related with the common steps of DNA damage response in Aspergillus nidulans.

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The Effects of uvsH Gene in Aspergillus nidulans on Mitotic Recombination Behabiour (Aspergillus nidulans에 있어서 uvsH 유전자가 mitotic recombination에 미치는 영향)

  • 채순기;한동민;강현삼
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.221-227
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    • 1986
  • The strain of Aspergillus nidulans carring a uvsH mutation which had been shown to be absolutely required for UV or 4-NQO induced mutagenic processes was studied on mitotic recombinational behaviour. Although the effect of uvsH locus on spontaneous mitotic crossing over between fpB37 and centromere was not considerable, UV-induced intergenic recombination did not occur in uvsH/uvsH homozygotic diploid. In case of gene conversion at riboflavin locus between a pair of non-complementary alleles, riboA1 and riboA3, the uvsH mutation was not concerned with that process occurred spontaneously or induced by UV irradiation. When the cells were irradiated by UV light, high degrees of aneuploid productions were detected in diploid homozygous for uvsH as compared with wild type, while much difference was not found during normal growth.

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Mapping of UV-B sensitive gene in Arabidopsis by CAPS markers (CAPS marker에 의한 Arabidopsis의 자외선 B 감수성 유전자 지도작성)

  • 박홍덕;김종봉
    • Journal of Life Science
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    • v.12 no.6
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    • pp.715-720
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    • 2002
  • A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.

Ultraviolet Lamp Replacement Period and Hygiene Management Plan of Ultraviolet Sterilizer (자외선 살균고의 자외선 램프 교체 주기와 위생관리 방안)

  • Young-Ju Lee;Ju-Hyun Lee;Eun-Sol Go;Jung-Beom Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.1
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    • pp.26-30
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    • 2023
  • In this study, we analyzed the microbial contamination level of ultraviolet sterilizer (UVS) chambers and suggested plans to improve hygiene management. In this study, UVS chambers targeted 98 UVS in some childcare centers in Jeollanam-do, Korea. Total aerobic bacteria and coliform bacteria were tested according to the Korean Food Code. Of the 98 UVS chambers, total aerobic bacteria were detected in 67 (68.4%) and coliform bacteria in 5 (5.1%). Six kinds of food-poisoning bacteria, including Salmonella spp., were not detected, but Bacillus cereus was detected in 1 (2.8%) out of 98 UVS chambers. According to the UV lamp replacement period, the detection rate of total aerobic bacteria was 3 (50%) out of 6 UVS within 3 months, 3 (60%) out of 5 UVS in 3 to 6 months, and 61 (70.1%) out of 87 UVS over 6 months. The detection rate of coliform bacteria according to the UV lamp replacement period was not detected within 6 months, however, they were detected in 5 (5.7%) out of 87 chambers after more than 6 months. The level of microbial contamination in the UVS chambers was higher as the lamp replacement period was longer. Considering these results, it was determined that the UVS chambers should be kept dry and clean, and the UV lamp should be replaced periodically. In addition, it is necessary to provide the staff catering for childcare centers with continuous education regarding the cleaning of UVS chambers and the replacement of UV lamps.

Distribution of Ultraviolet Intensity and UV Leaking of Commercial UV Sterilizers Used in Restaurants (음식점에서 사용하는 자외선 살균소독기 내 자외선 강도 분포 및 자외선 누출)

  • Mok, Chul-Kyoon;Lee, Nam-Hoon
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.228-233
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    • 2008
  • Ultraviolet sterilizers (UVS) are widely used in restaurants, cafeterias and catering businesses in Korea. The proper application of UVS, however, is still questionable since no studies have clearly identified their efficacy and safety, while regulations and regulatory management systems are yet to be established. In the present study, the efficacy of UVS were investigated by measuring spatial UV intensity inside five commercially operated UVS. The operating safety parameters were also checked by measuring leaked UV intensity. The UV intensities were inversely proportional to distance from the UV lamp within 25 cm, and to the square of the distance exceeding 25 cm. The UV intensities in commercial UVS varied with the distance from UV lamp and the incident angle, highlighting efficacy and stability concerns. Notable leakage was detected through the door gaps of a studied UVS, which also brought about safety concerns. Allowable working distances for the UV leaking UVS were suggested based on international standards.

Forecasting Market Demand of u-Transportation Vehicle Sensor OBU (u-Transportation UVS 단말기 시장수요예측)

  • Jeong, Eon-Su;Kim, Won-Kyu;Kim, Min-Heon;Kim, Byung-Jong;Kim, Song-Ju
    • Journal of The Institute of Information and Telecommunication Facilities Engineering
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    • v.8 no.4
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    • pp.157-162
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    • 2009
  • This study's purpose is to forecast the market demand of UVS (u-Transportation Vehicle Sensor) OBU (On-board Unit) of the ubiquitous Transportation. Bass model, Logistic model, and Gompertz model were used for the forecasting market demand. Firstly, this research focused on the market size for the u-T OBU. All three models were used for the market size prediction and the average values were used. The Bass model were calibrated and the market demand for the UVS OBU of the u-Transportation system were estimated using this model.

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An Automatic Segmentation System Based on HMM and Correction Algorithm (HMM 및 보정 알고리즘을 이용한 자동 음성 분할 시스템)

  • Kim, Mu-Jung;Kwon, Chul-Hong
    • Speech Sciences
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    • v.9 no.4
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    • pp.265-274
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    • 2002
  • In this paper we propose an automatic segmentation system that outputs the time alignment information of phoneme boundary using Viterbi search with HMM (Hidden Markov Model) and corrects these results by an UVS (unvoiced/voiced/silence) classification algorithm. We selecte a set of 39 monophones and a set of 647 extended phones for HMM models. For the UVS classification we use the feature parameters such as ZCR (Zero Crossing Rate), log energy, spectral distribution. The result of forced alignment using the extended phone set is 11% better than that of the monophone set. The UVS classification algorithm shows high performance to correct the segmentation results.

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