• Journal of Pharmaceutical Investigation
• /
• 제38권6호
• /
• pp.399-403
• /
• 2008

The aim of this study was to evaluate the difference of analytical methods for the pharmacokinetic study of bromosulfophthalein (BSP), an indicator of hepatobiliary function. The plasma and bile concentrations of BSP after intravenous administration were measured according to custom UV spectroscopy and HPLC, respectively. Plasma concentration of BSP measured by UV spectroscopy was similar to that measured by HPLC. There was no significant difference in the distribution volume, total body clearance, area under the curve and mean residence time of BSP between different analytical method groups. However, bile concentration of BSP measured by UV spectroscopy was overestimated compared with concentration measured by HPLC method. Biliary clearance of BSP obtained from UV spectroscopy method was almost 3 times higher than that obtained from HPLC method. Thus, a feasibility of UV spectroscopy method for high throughput pharmacokinetic evaluation of BSP was limited to the study based on the plasma concentration of BSP, not bile concentration.

• Tribology and Lubricants
• /
• 제35권3호
• /
• pp.151-157
• /
• 2019

Insulated oil degradation produces charged by-products, such as acids and hydro-peroxides, which tend to reduce the insulating properties of the oil. In this study, UV-vis spectroscopy measurement technology is developed and experimentally compared with other measurement methods, such as the titration method and IR spectroscopy, to validate its ability to monitor the degradation of electrical insulating paper. The degradation characteristics of the insulating paper are appropriately represented through various types of measurement methods, such as the Tan (delta) method, $CO_2$ gas production measurement, the titration method, and IR spectroscopy. The results are demonstrated to be well comparable to a change in the fluorescence emission ratio (FER), which is defined as the shift in fluorescence intensity in the measured wavelength range, and also to the chromatic ratio, which is defined as a color shift to longer wavelength ranges. The results also show that, by using UV-vis spectroscopy, it is possible to detect the degradation of the insulating paper. This study suggests that UV-vis spectroscopy can be applied as an alternative to high-performance liquid chromatography, which is the internationally recognized measurement technology for cellulose paper degradation. The FER detector is also verified to be useful as an effective condition-monitoring device for power transformers.

• Elastomers and Composites
• /
• 제49권2호
• /
• pp.155-159
• /
• 2014

1,2-dichlorobenzene용액에서 [$C_{70}$]풀러렌과 3-chloroperoxy benzoic acid 산화반응의 거동을 고분해능 초음파 분광기를 사용하여 고찰하였다. UV-vis spectroscopy, X-ray diffraction을 가지고 [$C_{70}$]풀러렌 산화 반응의 생성물을 확인하였다.

• Bulletin of the Korean Chemical Society
• /
• 제25권6호
• /
• pp.829-832
• /
• 2004

The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of ophenylenediamine-$H_2O_2$-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1 : 2 and the binding constant is to be $5.07{\times} 10^3L{\cdot}mol^{-1}$ at room temperature.

• 대한화학회지
• /
• 제38권2호
• /
• pp.169-173
• /
• 1994

인도네시아 Manado만의 Sulawesi에서 채집한 미동정된 해면으로부터 KB cancer cell line에 대해 활성을 갖는 xestoquinone, halenaquinol sulfate 및 halenaquinol이 검출되었다. 이들을 $^1H-,\;^{13}C$-NMR, $^1H-,\;^{13}C$(1 bond) heteronuclear Multiple Quantum Cogerence Spectroscopy$(HMQC)^1$, $^1H-,\;^{13}C$C(2 and 3 bond) Heteronuclear multiple Bond Correlation Spectroscopy$(HMBC)^2$, Electron Impact Mass Spectroscopy(EI ms), UV 및 IR에 의해 밝혔다.

• Bulletin of the Korean Chemical Society
• /
• 제25권8호
• /
• pp.1211-1216
• /
• 2004

The interaction of the antitumour agent doxorubicin with calf thymus DNA is investigated in an aqueous solution at a pH level of 6-7 with molar ratios of 1/10. A UV-resonance Raman spectroscopy and surface enhanced Raman spectroscopy are used to determine the doxorubicin binding sites and the structural variations of doxorubicin-DNA complexes in an aqueous solution. Doxorubicin intercalates with adenine and guanine via a hydrogen bond formation between the N7 positions of purine bases and the hydroxyl group of doxorubicin.

• 분석과학
• /
• 제13권3호
• /
• pp.282-290
• /
• 2000

본 연구는 390nm 이하에서 자외선 흡수가 완벽한 폴리카보네이트(광학용)에 첨가된 각종 첨가제를 아세톤으로 추출하거나 용해-침전 방식으로 분리하여 분석하는 것이다. 분리된 첨가제를 정성적으로 분석하기 위해 자외선흡광광도계, FT-IR 및 HPLC로 분석하였다. 분리된 물질은 광안정제 및 산화방지제(1차 및 2차)등의 첨가제와 단량체 및 올리고머가 함유된 복잡한 혼합물로 추정할 수 있다. 분리된 첨가물을 Bondapak $C_{18}$ 칼럼, 메탄올 이동상, PDA (Photodiode Array) 검출기를 사용한 역상(reversed phase, RP) HPLC 분석을 통해 비스페놀 A, Irganox 1010, Cyasorb UV-5411 성분들을 확인할 수 있있다. 또한, 자외선 흡광도를 이용한 정량분석을 통해 폴리카보네이트에 광안정제인 Cyasorb UV-5411가 0.12중량% 함유된 것을 알 수 있었다.

• Macromolecular Research
• /
• 제15권6호
• /
• pp.560-564
• /
• 2007

The photostabilization and cure kinetics of UV-curable, optical resins containing various formulations of photostabilizers were investigated to determine the system with the highest cure conversion and durability. Photo-DSC analysis revealed that increasing the concentration of a UV absorber (UVA) decreased both the crosslink density and the cure rate due to competition for the incident photons between the photoinitiator and the UVA, whereas including a hindered amine light stabilizer (HALS) hardly affected either the cure conversion or the cure rate due to its very low absorption of 365 nm. This result was confirmed by FTIR-ATR spectroscopy and UV-visible spectroscopy analyses. QUV ageing experiments showed that the cure conversion and durability were the highest for the UVA/HALS formulation at a ratio of 1 : 2, which is due to their synergistic action.

• 분석과학
• /
• 제10권2호
• /
• pp.139-145
• /
• 1997

합성 윤활기유로 사용되고 있는 알킬벤젠들 중에 단일, 그리고 이중치환된 알킬벤젠의 양과 알킬사슬에 존재하는 탄소원자의 수를 $^{13}C$-NMR, 근적외선 및 UV-Vis 분광법으로 분석하였다. 또한 엔진윤활유에 포함되어 있는 직선형 긴 사슬 알킬벤젠을 적외선분광법으로 분석하였다.

• 한국응용과학기술학회지
• /
• 제27권4호
• /
• pp.515-521
• /
• 2010

In this research, we were prepared the glazed ceramic samples by the change of the pigment content and investigated to reveal the standardized color. The chemical composition of the pigment was analyzed using a ICP-OES. XRF mapping and UV-Vis spectroscopy were used to evaluate the color property and surface homogeneity. The color development was observed as a result of analyzing the pigment content and change of the color. The pigment content and the spectral reflection showed the relation of the inverse proportion and the standardized color which could be revealed through analysis data.