Browse > Article
http://dx.doi.org/10.4333/KPS.2008.38.6.399

Effect of Analytical Method on the Pharmacokinetic Evaluation of Bromosulfophthalein: Comparison of HPLC and UV Spectroscopy Method  

Oh, Ju-Hee (College of Pharmacy, Kyung Hee University)
Cha, You-Kyoung (College of Pharmacy, Kyung Hee University)
Lee, Young-Joo (College of Pharmacy, Kyung Hee University)
Publication Information
Journal of Pharmaceutical Investigation / v.38, no.6, 2008 , pp. 399-403 More about this Journal
Abstract
The aim of this study was to evaluate the difference of analytical methods for the pharmacokinetic study of bromosulfophthalein (BSP), an indicator of hepatobiliary function. The plasma and bile concentrations of BSP after intravenous administration were measured according to custom UV spectroscopy and HPLC, respectively. Plasma concentration of BSP measured by UV spectroscopy was similar to that measured by HPLC. There was no significant difference in the distribution volume, total body clearance, area under the curve and mean residence time of BSP between different analytical method groups. However, bile concentration of BSP measured by UV spectroscopy was overestimated compared with concentration measured by HPLC method. Biliary clearance of BSP obtained from UV spectroscopy method was almost 3 times higher than that obtained from HPLC method. Thus, a feasibility of UV spectroscopy method for high throughput pharmacokinetic evaluation of BSP was limited to the study based on the plasma concentration of BSP, not bile concentration.
Keywords
Bromosulfophthalein; UV spectroscopy; HPLC; Biliary clearance;
Citations & Related Records
연도 인용수 순위
  • Reference
1 J. Konig, A.T. Nies, Y. Cui, I. Leier and D. Keppler, Conjugate export pumps of the multidrug resistance protein (MRP) family: localization, substrate specificity, and MRP2- mediated drug resistance, Biochim. Biophys. Acta., 1461, 377-394 (1999)   DOI   ScienceOn
2 M.A. Gonzalez, M.G. Roma, C.A. Bernal, L. Alvarez Mde and M.C. Carrillo, Biliary secretory function in rats chronically intoxicated with aluminum, Toxicol. Sci., 79, 189-195 (2004)   DOI   ScienceOn
3 B. Combes, The Importance of Conjugation with Glutathione for Sulfobromophthalein Sodium (Bsp) Transfer from Blood to Bile, J. Clin. Invest., 44, 1214-1224 (1965)   DOI   ScienceOn
4 K. Sano, Y. Totsuka, Y. Ikegami and T. Uesugi, Metabolism of sulphobromophthalein I: positional isomers of sulphobromophthalein monoglutathione conjugate, J. Pharm. Pharmacol., 53, 1015-1020 (2001)   DOI   ScienceOn
5 B.G. Priestly and G.L. Plaa, Sulfobromophthalein metabolism and excretion in rats with iodomethane-induced depletion of hepatic glutathione, J. Pharmacol. Exp. Ther., 174, 221-231 (1970)
6 K.K. Machavaram, J. Gundu and M.R. Yamsani, Effect of various cytochrome P450 3A and P-glycoprotein modulators on the biliary clearance of bromosulphaphthalein in male wistar rats, Pharmazie, 59, 957-960 (2004)
7 C.A. Snel, M.M. Moons, F.G. Russel and G.J. Mulder, Disposition of the bromosulfophthalein-glutathione conjugate in the isolated perfused rat kidney, J. Pharmacol. Exp. Ther., 273, 1300-1306 (1995)
8 M.L. Rocci, and W.J. Jusko, LAGRAN program for area and moments in pharmacokinetic analysis, Comput. Programs. Biomed., 16, 203-216 (1983)   DOI   ScienceOn
9 G. Whelan, J. Hoch and B. Combes, A direct assessment of the importance of conjugation for biliary transport of sulfobromophthalein sodium, J. Lab. Clin. Med., 75, 542-557 (1970)
10 K. Sano, Y. Totsuka, Y. Ikegami and T. Uesugi, Metabolism of sulphobromophthalein II. Species differences between rats, guinea-pigs and rabbits, J. Pharm. Pharmacol., 54, 231-239 (2002)   DOI   ScienceOn
11 S. Itagaki, M. Sugawara, M. Kobayashi, K. Miyazaki, T. Hirano and K. Iseki, Comparison of the disposition behavior of organic anions in an animal model for Wilson's disease (Long-Evans Cinnamon rats) with that in normal Long- Evans Agouti rats, Drug. Metab. Pharmacokinet., 19, 150-154 (2004)   DOI   ScienceOn
12 S. Tazuma, K. Horikawa, H. Ochi, T. Nishioka, Y. Sunami, S. Yasumiba, Y. Asamoto, K. Tsuboi, K. Nakai, M. Sakomoto, K. Kanno, A. Yamaguchi, Y. Numata and K. Chayama, Dose-dependent conjugation of sulfobromophthalein and hepatic transit time in bile fistula rats: role of the microtubule-dependent vesicle pathway, Dig. Dis. Sci., 46, 1285-1289 (2001)   DOI   ScienceOn
13 C.C. Paulusma, M.A. van Geer, R. Evers, M. Heijn, R. Ottenhoff, P. Borst and R.P. Oude Elferink, Canalicular multispecific organic anion transporter/multidrug resistance protein 2 mediates low-affinity transport of reduced glutathione, Biochem. J., 338, 393-401 (1999)   DOI