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http://dx.doi.org/10.5012/bkcs.2004.25.6.829

Electrochemical and Spectroscopic Studies on the Interaction between DNA and the Product of Enzyme-catalyzed Reaction of OPD-H2O2-HRP  

Niu, S.Y. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
Zhang, S.S. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
Ma, L.B. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
Jiao, K. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
Publication Information
Bulletin of the Korean Chemical Society / v.25, no.6, 2004 , pp. 829-832 More about this Journal
Abstract
The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of ophenylenediamine-$H_2O_2$-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1 : 2 and the binding constant is to be $5.07{\times} 10^3L{\cdot}mol^{-1}$ at room temperature.
Keywords
2,3-Diaminophenazine; DNA; Cyclic voltammetry; UV/Vis spectroscopy; Intercalation;
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