• Title/Summary/Keyword: UV peak

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Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons (염화 방향족 탄화수소 분해세균의 분리 및 특성)

  • 김종우;김치경;김영창;염재홍;이재구
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.122-128
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    • 1987
  • Several bacterial isolates capable of degrading 4-chlorobiphenyl or 2,4,5-trichlorophenoxyacetic acid were isolated from industrial wastes by the agar plate method and studied for their biodegradabilities of the hydrocarbons and some biochemical characteristics. The isolates DJ-12, DJ-26 and TP-1 were identified as Pseudomonas spp. and they could not degrade 2,4-dichlorophenoxyacetic acid. The absorption spectra for 4-chlorobiphenyl and 2,4,5-trichlorophenoxyacetic acid showed the peaks at 253 and 292 nm, respectively. Biodegradability of the isolates was determined by decrease of the absorbance for the test hydrocarbons with a UV-scanning spectrophotometer. The plasmids of the isolates were studied to examine whether or not the hydrocarbon-degrading genes exist in the plasmids. Antibiotics resistance was also examined to search out a proper marker for the isolates in further experiments, such as curing test and genetic recombination.

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Magellan High Resolution Spectroscopy of Raman-Scattered He II, C II and O VI Lines in the Symbiotic Nova RR Telescopii

  • Heo, Jeong-Eun;Lee, Hee-Won;Di Mille, Francesco;Palma, Tali;Angeloni, Rodolfo
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.87.2-87.2
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    • 2017
  • RR~Telescopii is a symbiotic nova exhibiting accretion activities through gravitational capture of the slow stellar wind from a Mira variable. We present high resolution spectra of RR~Tel obtained with MIKE and the 6.5 m Magellan-Clay telescope, in which we find broad features with FWHM exceeding $10{\AA}$ at 6545, 6825, 7082, 7023 and $7053{\AA}$. They are formed through Raman-scattering with atomic hydrogen of far-UV He II 1025, O~VI 1032, $1038{\AA}$ and C II 1036 and $1037{\AA}$. We compute the Raman conversion efficiencies using the case B recombination theory for He II emissions, which are used in turn to infer the intrinsic line luminosities of O VI and C II. The Raman O~VI features are characterized by double-peaked profiles with a peak separation ~ 60km/s, pointing out the presence of an accretion disk with a physical size of ~ sub AU. In contrast, Raman C II features exhibit profiles with a simple peak and a narrower width ~40 km/s, indicating that C II is formed in a much more extended region. The weak C II multiplet at 1335, $1336{\AA}$ found in the IUE spectral archive and the absence of C II 1036, $1037{\AA}$ in the FUSE archive show that far-UV C II lines suffer heavy interstellar extinction consistent with the distance of ~ 2.5 kpc to RR Tel.

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Physicochemical Characteristics and Pharmacokintics of Ibuprofen Lysine Salts (흰쥐에서 이부프로펜 리신염의 물리화학적 특성 및 약물동태에 관한 평가)

  • Shin, Dae-Hwan;Kim, Tai-Sung;Park, Seong-Hyeok;Kim, Si-Hyun;Jo, Han-Jun;Han, Kun;Chung, Youn-Bok
    • YAKHAK HOEJI
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    • v.55 no.3
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    • pp.260-266
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    • 2011
  • Two types of water soluble lysine salts of ibuprofen were prepared and evaluated. Physicochemical properties for ibuprofen-l-lysinate (IBL-l), ibuprofen-dl-lysinate (IBL-dl) and ibuprofen (IB) were studied on melting point, specific ratation, UV spectra and $^1H$-NMR spectra. There were not differences between IBL-dl and IBL-l in UV spectra and $^1H$-NMR spectra. The pharmacokinetic parameters of IB were compared to those of its lysine salts (IBL-l and IBL-dl) after i.v. or oral administration at the dose of 50 mg/kg (calculated as IB). Total body clearance ($CL_t$) and area under the plasma concentration-time curve (AUC) were not different between IB group and IBL groups after i.v. administration. On the other hand, IBL-l and IBL-dl produced peak plasma concentrations ($C_{max}$) significantly ealier and higher than IB. Time to reach peak concentration ($T_{max}$) after IBL administration was lower than that after IB administration. There was no difference in AUC across all different groups (IB, IBL-l and IBL-dl) after oral administration. However, absorption rate constant ($k_a$) of IBL-l and IBL-dl were significantly increased than that of IB. These results indicated that the administration of IBL-l and IBL-dl may be advantageous if rapid and reliable onset of pain relief is required.

Synthesis of New Spiro[benzo[c]fluorene-7,9'-fluorene] Dimers and Their Optical Properties

  • Seo, Jeong-A;Lee, Chil-Won;Gong, Myoung-Seon
    • Bulletin of the Korean Chemical Society
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    • v.34 no.5
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    • pp.1414-1420
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    • 2013
  • Five novel spiro[benzo[c]fluorene-7,9'-fluorene] based dyes, including 5-[spiro[benzo[c]fluorene-7,9'-fluoren]-5-yl] spiro[benzo[c]fluorene-7,9'-fluorene] (7), 5-[spiro[benzo[c]fluorene-7,9'-fluoren]-9-yl] spiro[benzo[c]fluorene-7,9'-fluorene] (8), 5-[spiro[benzo[c]fluorene-7,9'-fluoren]-2'-yl] spiro[benzo[c]fluorene-7,9'-fluorene] (9), 9-[spiro[benzo[c]fluorene-7,9'-fluoren]-9-yl] spiro[benzo[c]fluorene-7,9'-fluorene] (10), and 2'-[spiro[benzo[c]-fluorene-7,9'-fluoren]-2'-yl] spiro[benzo[c]fluorene-7,9'-fluorene] (11) were successfully prepared from the corresponding halogen and boronic acid derivatives through the Suzuki coupling reaction, respectively. Chemical structures were confirmed by $^1H$ nuclear magnetic resonance (NMR), $^{13}C$ NMR, Fourier transforminfrared spectrscopy, mass spectroscopy, and elemental analysis. The thermal properties were determined by differential scanning calorimetry and thermal gravimetric analysis. The relationships between the optical and electrochemical properties and the combined positions between these dimers were systematically investigated using UV-vis, photoluminescence (PL), and photoelectron spectroscopy. These five dimers exhibited high fluorescent quantum yields and good morphological stability with high glass transition states > $174^{\circ}C$. Dimer 7 showed a UV absorbance peak at 353 nm, emission PL peak at 424 nm, and quantum efficiency of 0.62 in a cyclohexane solution.

Antioxidative Characteristics of Browning Reaction Products of Glucose-Poly-${\gamma}$-Glutamate (GIu-PGA) obtained from Amino-carbonyl Reaction (Amino-carbonyl 반응에 의한 glucose-poly-${\gamma}$-glutamate (Glu-PGA) 갈변 반응물질의 항산화적 특성 연구)

  • Lee, Nam-Keun;Hahm, Young-Tae
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.812-815
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    • 2005
  • Effects of poly-${\gamma}$-glutamate (PGA) on antioxidative characteristics of amino-carbonyl reaction products of glucose were investigated. Rapid browning reaction was observed under heat and alkaline condition (pH 8.2). Browning products were separated by Sephadex G-50, and brown color intensity and electron-donating ability of DPPH (2,2-diphenyl-1-picrylhydrazyl) of each fraction were measured. Fraction-7 (F-7) and -20 (F-20) showed high DPPH scavenging values. UV-VIS absorption spectrum of F-20 was similar to melanoidin peak, and F-7 showed maximum absorption peak at 270 nm. Molecular weight of F-7 was over 35 kDa as determined by SDS-PAGE. Although F-20 could not be measured on SDS-PAGE, its size was smaller than F-7.

Synthesis, Film Fabrication, and Optical Properties of Polymers Containing Metal Cation Complex Type D-$\pi$-A Chromophore (금속 양이온 배위형 D-$\pi$-A 발색단을 포함하는 폴리머의 합성 및 박막화와 광학특성)

  • Jeong, Seon-Ju;Kim, Hye-Ryun;Yoon, Keun-Byoung;Han, Yoon-Soo;Fujiki, Michiya;Takagi, Akiko;Kwak, Gi-Seop
    • Polymer(Korea)
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    • v.34 no.4
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    • pp.376-380
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    • 2010
  • Donor-$\pi$-acceptor (D-$\pi$-A) type chromophore-based polymers were newly synthesized. These polymers exhibited absorption peak due to intramolecular charge transfer (ICT) in a visible range as well as absorption peak due to carbonyl group in both solution and film state by measuring UV visible spectra. The addition of $Eu^{3+}$ ion into the polymers induced red-shift in absorption due to ICT and the color changes from yellow to red in the solution and film were observed by naked eyes. The contents of crosslinking agent influenced the features and solubility of the polymers. In addition, the contents of crosslinking agent and the $Eu^{3+}$ ion addition improved film-forming ability.

Influence of UV Irradiation Interval on Mineral Content in Metatarsus of Broiler Chicks (자외선 조사간격이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향)

  • 장윤환;조인호;여영수;이은택;배은경;김중달
    • Korean Journal of Poultry Science
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    • v.20 no.3
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    • pp.133-140
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    • 1993
  • A study was conducted to investigate the concentrations of Ca, P and ash in metatarsal bone of broiler chicks exposed to UV light in different Interval. Day-old Hubbard broiler chicks (199=10 control+3 irradiation interval $\times$ 9 elapsed time $\times$ 7 replicate) were fed vitamin D3 deficient diet for 3 wk in a windowless subdued-light room and exposed to 297 nm UVB light by 0.068 mJ/$\textrm{cm}^2$ three times In 0, 12 or 24 h interval. The metatarsal bones were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, separated from adhering tissue, ether extracted, dried and ashed. The Ca concentration was measured by atomic absorption spectrophotometry and P by ammonium metavanadate colorimetry. When the birds were continuously exposed to UVB light for 30 min without interval, the Ca content in metatarsus increased gradually according to the time after irradiation and reached the highest value 16.75% at 240 h after exposure. The P content also increased gradually until 144 h, where it was 9.75%. The ash content in metatarsus increased continuously until 240 h, the final time in this research, where 42.75% was shown. As 10 min three times irradiation in 12 h interval was applied to the chicks, the metatarsal Ca presented a small peak(13.31%) at 12 h after irradiation and a large peak(16.91%) at 144 h. P content showed a small peak(7.18%) at 12 h and a large level(8.34%) at 240 h. Ash content increased continuously until 240 h, where it was 46.53%. The small peaks in Ca and P concentration were thought to be derived from preirradiation at 12 and 24 h before final irradiation for 10 min. When 24 h interval system was treated, the peak value of Ca content(24.18%) occurred earlier(96 h) than those in 0 and 12 h interval systems. P content also showed the maximum value at 96 h(7.29%). Ash content presented an increasing trend until 240 h, where 45.75% was appeared. In respecting the method of UVB irradiation, the peak value of Ca content in metatarsus appeared earlier in 24 h interval system than in other systems. Meanwhile the ash contents in metatarsus of birds exposed to UVB light in 12 and 24 h interval procedures were higher than those in 0 h interval one. Therefore, it was concluded that a daily 10 min irradiation of UVB light would be desirable for increasing the Ca and ash content in metatarsus of brolier chicks.

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A study of defect structures in $LiNbO_{3}$ single crystals by optical absorptions (광흡수에 의한 $LiNbO_{3}$ 단결정의 결함 구조 연구)

  • 김상수
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.327-340
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    • 1996
  • In this study, a series of $LiNbO_{3}$ crystals with different [Li]/[Nb] ratios, congruent $LiNbO_{3}$ crystals with doped Mg and with Mg and codoped with Mn were grown by the Czocharalski method. These were investigated by UV and IR spectrophotometry. Stoichiometry dependences of the UV absorption edge and the $OH^{-}$ absorption spectra were studied with different [Li]/[Nb] ratios. The position of the UV absorption edge adn the shape and peak point of the $OH^{-}$ absorption spectra changed monotonously upto a critical concentration of Mg ions. The mechanism of the incorporation of Mg ions changes at this concentration. The decomposition of the $OH^{-}$ absorption spectra using a Gaussian lineshape function showed that in Li-deficient crystals the absorption spectra consist of five components in contrast to more or less perfect stoichiometric crystals which reveal to three components. On the basis of these results, the intrinsic and the extrinsic defect structure models in $LiNbO_{3}$ crystals were examined. The behaviour of $\nu$ (OH) reflects the defect structure and supports the Li-site vacancy model as the intrinsic defect structure model and the corresponding extrinsic defect model. A brief discussion is also given of the behaviour of $\nu$ (OH) in $LiNbO_{3}$ crystals simultaneously doped with several kinds of impurity.

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Effect of Substrate Temperature on the Emission Characteristics of ZnO Films Grown by Pulsed Laser Deposition (기판 온도의 영향에 따른 펄스레이저 증착법으로 성장된 ZnO 박막의 발광 특성)

  • Kim, Y.H.;Kim, S.I.
    • Journal of the Korean Vacuum Society
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    • v.18 no.5
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    • pp.358-364
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    • 2009
  • We investigated the growth of ZnO thin films with prominent emission characteristics through minimizing the formation of defects by using pulsed laser deposition (PLD). To do so, the ZnO films were deposited on sapphire(0001) substrates at the substrate temperature of $400-850^{\circ}C$ and then the variation of their structural and optical properties were analyzed by x-ray diffraction, atomic force microscope and photoluminescence. As a result, all ZnO films were grown with c-axis preferential orientation irrespective of the substrate temperature. However, the crystallinity and stress state were dependent on the substrate temperature and the ZnO film deposited at $600^{\circ}C$ showed the best surface morphology and crystallinity with nearly no strain. And also this film exhibited outstanding emission characteristics from the viewpoint of full width half maximum of UV emission peak as well as visible emission due to defects. These results indicate that the emission characteristics of the ZnO films are strongly related to their structural characteristics influenced by substrate temperature. Consequently, ZnO films with strong UV emission and nearly no visible emission, which are applicable to UV emission devices, could be grown at the substrate temperature of $600^{\circ}C$ by PLD.

Application of Quantum-dot Nanocrystals for Cyanobacterial Toxin-Microcystin Detection (나노크리스탈 Quantum-dot을 적용한 남조류 독소 Microcystin 탐지 연구)

  • Lee, Jinwook;Yu, Hye-Weon;Kim, In S.
    • Journal of Korean Society on Water Environment
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    • v.23 no.5
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    • pp.705-711
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    • 2007
  • Green quantum-dot nanocrystal (QD525) with anti-microcystin monoclonal antibody was applied for detection of microcystin, a monocyclic peptide hepatotoxin, extracted from the culture of Microcystis aeruginosa. The presence of microcystin in the cell lysate was verified by HPLC analysis with UV absorbance at 238 nm. Microcystis cell extract exhibited fluorescence emission spectra, which peak was around 460 nm because of their complex organic substances. When a spherical QD525 antibody conjugates (10~20 nm in diameter) were bound to the microcystins in the Microcystis cell lysate, the fluorescence intensity of the primary peak at 525 nm diminished while the secondary emission peak at 460 nm slightly increased intensities. It is due to energy transfer from the primary (major) to the secondary (minor) peak, resulting from physical deformation of QD525 and different environmental factors. On the other hand, other cell extracts did not show any fluorescence emission change. This study is very available for detecting and monitoring the microcystin because it is one step assay without washing step and portable spectrophotometer makes on-site measurement possible. For health risk assessment of the microcystin, the reliable and rapid system to detect and quantify microcystin is seriously required.