Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Application of Quantum-dot Nanocrystals for Cyanobacterial Toxin-Microcystin Detection

나노크리스탈 Quantum-dot을 적용한 남조류 독소 Microcystin 탐지 연구

  • Lee, Jinwook (Bio-Environmental Engineering Lab. (BEEL), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Yu, Hye-Weon (Bio-Environmental Engineering Lab. (BEEL), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Kim, In S. (Bio-Environmental Engineering Lab. (BEEL), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST))
  • 이진욱 (광주과학기술원 환경공학과 BEEL연구실) ;
  • 유혜원 (광주과학기술원 환경공학과 BEEL연구실) ;
  • 김인수 (광주과학기술원 환경공학과 BEEL연구실)
  • Received : 2007.07.11
  • Accepted : 2007.09.14
  • Published : 2007.09.30
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Abstract

Green quantum-dot nanocrystal (QD525) with anti-microcystin monoclonal antibody was applied for detection of microcystin, a monocyclic peptide hepatotoxin, extracted from the culture of Microcystis aeruginosa. The presence of microcystin in the cell lysate was verified by HPLC analysis with UV absorbance at 238 nm. Microcystis cell extract exhibited fluorescence emission spectra, which peak was around 460 nm because of their complex organic substances. When a spherical QD525 antibody conjugates (10~20 nm in diameter) were bound to the microcystins in the Microcystis cell lysate, the fluorescence intensity of the primary peak at 525 nm diminished while the secondary emission peak at 460 nm slightly increased intensities. It is due to energy transfer from the primary (major) to the secondary (minor) peak, resulting from physical deformation of QD525 and different environmental factors. On the other hand, other cell extracts did not show any fluorescence emission change. This study is very available for detecting and monitoring the microcystin because it is one step assay without washing step and portable spectrophotometer makes on-site measurement possible. For health risk assessment of the microcystin, the reliable and rapid system to detect and quantify microcystin is seriously required.

Keywords

Acknowledgement

Supported by : 광주과학기술원

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