• Journal of Microbiology and Biotechnology
• /
• v.16 no.6
• /
• pp.979-982
• /
• 2006

Two knockout vectors, in which the truncated Kluyveromyces lactis URAS gene is flanked by a direct repeat, were developed for Saccharomyces cerevisiae. Each vector was designed to harbor 5'- and 3'-end homology regions for integration. Two knockout fragments were devised to integrate into the correct locus in a complementary manner to disrupt a gene of interest and. concomitantly to make functional Kl URA3 for transfomant selection. The use of dual complementary knockout cassettes was expected to dramatically reduce integration into unwanted loci in the genome. The knockout system developed in this study was successfully used for disruption of the GAL1 gene in S. cerevisiae.

• Journal of the Korean Society of Tobacco Science
• /
• v.23 no.2
• /
• pp.133-140
• /
• 2001

PAP-I cDNA was synthesized from total RNA of Phytolacca americana leaves by RT-PCR, and then subcloned to recombinant vector pBluescript II SK-. Using PCR with primers designed in our laboratory, we could get the 9 deletion mutant PAP-I cDNA fragments. The first of the fragments was deleted by 66bp from immature N-terminal and then the rest were deleted by 90bp sequentially. Sequentially deletion mutant PAP-I cDNAs were inserted to pAc55M, on down-stream of gall promoter. Recombinant pAc55M was transformed to yeast cells, psy1 and the cells were spreaded on SC_urn-/glucose plate media. Colonies on SC_ura-/glucose plate were streaked on the same position of SC_ura-/glucose and SC_ura-/galactose plate, and we selected colonies growing on both plates, which carry non-cytotoxic deleted mutant PAP-I cDNA. We selected 4 deletion mutant PAP-I cDNAs which have not cytotoxicity.

• Asian-Australasian Journal of Animal Sciences
• /
• v.2 no.3
• /
• pp.456-457
• /
• 1989

• Journal of the Korea Convergence Society
• /
• v.10 no.2
• /
• pp.309-313
• /
• 2019

This study examined the response of the EMG of URA, LRA, IO, EO and RFM of various types of abdominal motion (crunch, spine V-up on ball, prone V-up on slide board, prone V-up on TRX, and prone V-up power wheel). The subjects performed anisometric contact of abs during these exercises. Tests have shown that there were no statistically significant differences between EO, URA and LRA between any movements. However, during the inspection of IO, the positive-wawed V-up motion showed significantly greater muscle activity than during the slide movement. Also, EMG activity during crunch was significantly lower than any other five exercises. These results indicate that in the implementation of equilateral absolutism, the equipment-free based exercise gives an impetus similar to equipment-based exercise. Abdominal muscle tissue is considered one of the five components that make up an individual's core. The abdominal muscles also ensure proper functioning of the lumbar spine. Although all abdominal muscles contribute to lumbar stabilization, TA & IO has been shown to perform major stabilizers.

• Applied Biological Chemistry
• /
• v.28 no.1
• /
• pp.13-18
• /
• 1985

To investigate the fusion of bacterial protoplasts, two auxotrophic mutants of Bacillus subtilis were isolated after treatment with nitrosoguanidine. Two auxotrophs were uracil-auxotroph and tryptophan-auxotroph. Back mutation frequencies of $ura^-$ and $trp^-$ -auxotrophs were $2.4{\times}10^{-8}$ and less than $1.0{\times}10^{-8}$, respectively. The optimal pH and temperature of protoplast formation with lysozyme were 6.5 and $30^{\circ}C$. The optimal lysozyme concentration was $200{\mu}g/ml$. The regeneration frequency of protoplast was 3.3%.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• v.2
• /
• pp.143-148
• /
• 2006

The goal of this paper is to analyze, through simulations and experiments, GNSS interference mitigation performance under various types of antenna structures against wideband and narrowband interferences using spatial-temporal adaptive signal processing (STAP) techniques. The STAP approach, which combines spatial and temporal processing, is a viable means of GNSS array signal processing that enhancing the desired signal quality and providing protection against interference. In this paper, we consider four types of 3D antenna array structure - Uniform Linear Array (ULA), Uniform Rectangular Array (URA), Uniform Circular Array (UCA), and the Single-Ring Cylindrical Array (SRCA) under an interference environment. Analytical evaluation and simulations are performed to investigate the system performance. This is followed by simulation GPS orbits in interfered environment are used to evaluate the STAP performance. Furthermore, experiments using a 2x2 URA hardware simulator data show that with the removal of wideband and narrowband interference through the STAP techniques, the signal tracking performance can be enhanced.

• ALGAE
• /
• v.22 no.1
• /
• pp.37-44
• /
• 2007

In order to understand the impact of the heavy-oil pollution by the 1997 Nakhodka oil spill on the intertidal macroalgal vegetation, we have been monitoring succession in the intertidal flora since 1997 at Oh-ura, Takno, and Imago-Ura Cove, Kasumi in Hyogo Prefecture, northwestern coast of Honshu, Japan. We employed two different monitoring methods: 1) The percent cover of macro-algae (seaweeds) in 1 x 1 m quadrats along 450 m intertidal transects parallel to the shoreline were assessed and recorded by photographic imaging until 2002, and for 30-40 m transects of the most heavily polluted areas in 2004 and 2006; 2) The percent cover of macro-algae in 0.5 x 0.5 m quadrats along a transect line perpendicular to the shore were recorded and all macrophytes within the quadrat were completely removed to record the wet weight of each taxon (1997-2006). Based on the monitoring data, we conclude that the high intertidal zone at Imago-ura, where a large part of the stranded oil accumulated, suffered the heaviest damage and experienced the slowest recovery. In addition, although the original status of macroalgal vegetation before the impact was not well-documented, it appeared that recovery from the damage caused by the oil pollution required four to five years.

• Molecular & Cellular Toxicology
• /
• v.4 no.4
• /
• pp.338-347
• /
• 2008

Yeast RAD2, a yeast homolog of human XPG gene, is an essential element of nucleotide excision repair (NER), and its deletion confers UV sensitivity and NER deficiency. 6-Azauracil (6AU) sensitivity of certain rad2 mutants revealed that RAD2 has transcription elongation function. However, the fundamental mechanism by which the rad2 mutations confer 6AU sensitivity was not clearly elucidated yet. Using an insertional mutagenesis, PUF4 gene encoding a yeast pumilio protein was identified as a deletion suppressor of rad2${\Delta}$ 6AU sensitivity. Microarray analysis followed by confirmatory RT-qPCR disclosed that RAD2 and PUF4 regulated expression of HPT1 and URA3. Overexpression of HPT1 and URA3 rescued the 6AU sensitivity of rad2${\Delta}$ and puf4${\Delta}$ mutants. These results indicate that 6AU sensitivity of rad2 mutants is in part ascribed to impaired expression regulation of genes in the nucleotide metabolism. Based on the results, the possible connection between impaired transcription elongation function of RAD2/XPG and Cockayne syndrome via PUF4 is discussed.

• Informatization Policy
• /
• v.24 no.4
• /
• pp.44-67
• /
• 2017

Commercial properties are gaining importance in the national economy, drawing more and more interests from agencies and individuals. Therefore, objective and accurate investment information needs to be supplied in order to support rational decision making and market transparency. To contribute to guiding the Korean commercial property market to become as transparent and competitive as the markets of developed countries, this study aims to seek for ways to establish a Korean commercial property information framework. As part of the activities to achieve the goal, the Korean commercial property market information was first evaluated in terms of data collection, production and operation. Then, US NCREIF, which is operated by the private sector, and Singapore's URA, managed by the public sector, were considered as the examples of well-managed foreign commercial property market information frameworks. Efficient management of the system requires establishing a monitoring scheme, and users' accessibility and information availability will be enhanced through phased expansion of the information.

• The Korean Journal of Mycology
• /
• v.17 no.4
• /
• pp.209-213
• /
• 1989

Transformation of an auxotrophic requirement for uracil in Pleurotus florida P101 has been achieved using chimeric vector containing Aspergillus nidulans ans 1, and Neurospora crassa pyr 4 DNA. Protoplasts of $Ura^-$strains of P. florida were incubated with plasmid pDJB3 containing the cloned pyr 4 gene in the presence of polyethylene glycol and $CaCl_2$. Transformants could grow on MMM showing mitotical stability. Southern hybridization analysis of DNA isolated from transformants showed that the Neurospora pyr 4 gene and vector sequence might be integrated into the P. florida chromosomes. As the transformants were monokaryon, each transformant was mated with the other monokaryon. Fruitbody shape of untransformant was eroded type but those of transformants were eroded type, funnel type, plane type and ungrowing cap type.