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A Study on the Analysis of Five Artificial Sweetners in Beverages by HPLC/MS/MS (HPLC/MS/MS를 이용한 음료류 중 인공감미료 동시분석에 관한 연구)

  • Lee, Seong-Bong;Yong, Kum-Chan;Hwang, Sun-Il;Kim, Young-Su;Jung, You-Jung;Seo, Mi-Young;Lee, Chang-Hee;Sung, Jin-Hee;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.327-333
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    • 2014
  • A method for analysis of five artificial sweetners (sodium saccharin, aspartame, acesulfame-K, sucralose, cyclamate) in beverage samples was developed using high-performance liquid chromatography/triple quadrupole mass spectrometry (HPLC/MS/MS). The method uses a single-step dilution for sample preperation. Seperation was achieved on a $C_{18}$ column ($2.1{\times}150mm$, $3.5{\mu}m$) with A- 2% methanol (1 mM ammonium acetate), B-95% methanol (1 mM ammonium acetate) as mobile phase with gradient mode. The quantitation of target compounds was performed by external calibration in selected reaction monitorning (SRM) mode. The coefficient of determination of calibration curve for sodium saccharin, aspartame, acesulfame-K, sucralose and cyclamate were 0.9957, 0.9991, 0.9943, 0.9982 and 0.9948, respectively. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0.001~0.022 mg/L and 0.004~0.073 mg/L, repectively. Recoveries for beverage samples were in the range of 92.76~113.50% with RSD < 10.91%. The method has applied to the determination of the five sweetners in 102 beverage samples. Three artificial sweetners-aspartame, acesulfame-K, sucralose were detected from 42 samples. Sodium saccharin and cyclamate were not detected in all samples.

Der Verlust der Amtsfähigkeit bzw. des Wahlrechts und das Gebot der Individualisierung der Strafen (선거범에 대한 자격제한과 형벌개별화원칙)

  • Chung, Kwang-Hyun
    • Journal of Legislation Research
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    • no.53
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    • pp.337-374
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    • 2017
  • Wer wegen eines Wahldelikts zu Geldstrafe von mehr als 1 Million Won verurteilt wird, verliert nach ${\S}$ 18 Abs. 1 Satz 3, ${\S}$ 19 Satz 1, ${\S}$ 266 des Koreanischen Wahlgesetzes $f{\ddot{u}}r$ die Dauer von $f{\ddot{u}}nf$ Jahren die $F{\ddot{a}}higkeit$, ${\ddot{o}}ffentliche$ ${\ddot{A}}mter$ zu bekleiden und Rechte aus ${\ddot{o}}ffentlichen$ Wahlen. Bei Verurteilung zu Freiheitsstrafe wegen eines Wahldelikts ${\ddot{A}}mter$ sich die Dauer des Verlusts der $Amtsf{\ddot{a}}higkeit$ und des aktiven bzw. passiven Walhrechts auf 10 Jahre. Dies erfolgt kraft Gesetzes. Das $hei{\ss}t$, dass die Entscheidung ${\ddot{u}}ber$ das Ob und die Dauer des Verlusts nicht im Ermessen des Gerichts steht. Allerdings sollte $diesbez{\ddot{u}}glich$ nicht verkannt werden, dass ein deratriger Entzug von $Amtsf{\ddot{a}}higkeit$, $W{\ddot{a}}hlbarkeit$ u.s.w., mit dem eine Straftat geahndet werden soll, selber von Natur aus eine Art Strafen darstellt. Der im ${\S}$ 41 des Koreanischen StGB geregelte Strafen-Katalog $enth{\ddot{a}}lt$ $n{\ddot{a}}mlich$ eine zeitlich begrenzte Aberkennung des oben genannten ${\ddot{o}}ffentliche$n Rechtsstatus als eine Art Ehrenstrafen. Nicht einleuchtend ist, warum das Wesen der Sanktion $gem{\ddot{a}}{\ss}$ ${\S}$ 18 Abs. 1 Satz 3, ${\S}$ 19 Satz 1, ${\S}$ 266 des Koreanischen Wahlgesetzes, die den gleichen Zweck und die gleiche Rechtsfolge wie die im ${\S}$ 41 des Koreanischen StGB geregelte Ehrenstrafe hat, nicht als Strafe aufgefasst werden sollte. Handelt es sich bei der oben genannten Sanktion um eine Art Ehrenstrafen, so stellt sich die Anforderung, sie je nach der Eigenart der begangenen Tat bzw. des $T{\ddot{a}}ters$ zu individualisieren. Das Gebot der Individualisierung der Strafen, welches $haupts{\ddot{a}}chlich$ vom materiellen Rechtsstaatsprinzip ableitbar ist, kann im Grunde nur verwirklicht werden, wenn das Gericht dazu befugt ist, unter $Ber{\ddot{u}}cksichtigung$ der konkreten $Umst{\ddot{a}}nde$ jedes Einzelfalls ${\ddot{u}}ber$ eine angemessene Strafe zu befinden. Somit ist der kraft Gesetzes eintretenden Verlust der $Amtsf{\ddot{a}}higkeit$ und der $W{\ddot{a}}hlbarkeit$ nur schwer mit dem Gebot der Individualisierung der Strafen vereinbar. Es $w{\ddot{a}}re$ deshalb $w{\ddot{u}}nschenswert$, wenn der Gesetzgeber eine Reform in Betracht ziehen $w{\ddot{u}}rde$, welche den Ersatz des kraft Gesetzes automatisch eintretenden Entzugs der $Amtsf{\ddot{a}}higkeit$ bzw. des Wahlrechts durch die gerichtliche fakultative Aberkennung von diesen Statusrechten beinhaltet.

Comparison of γ-aminobutyric acid and isoflavone aglycone contents, to radical scavenging activities of high-protein soybean sprouting by lactic acid fermentation with Lactobacillus brevis (발아 고단백 콩의 Lactobacillus brevis 젖산발효에 의한 가바와 이소플라본 함량 및 라디칼 소거활성의 비교)

  • Hwang, Chung Eun;Haque, Md. Azizul;Lee, Jin Hwan;Joo, Ok Soo;Kim, Su Cheol;Lee, Hee Yul;Um, Bong Sik;Park, Kyung Sook;Cho, Kye Man
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.7-18
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    • 2018
  • In this study, soy-powder yogurt (SPY) with enhanced levels of ${\gamma}$-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and $491.25{\mu}g/g$ in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

Suitability Grouping System of Paddy Soils for Multiple Cropping -Part I: Basic Experiments (다모작(多毛作)을 위한 답토양(畓土壤) 적성등급(適性等級) 구분(區分) -제(第) 1 보(報) : 기초시험(基礎試驗))

  • Jung, Yeun-Tae;Park, Eun-Ho;No, Yeong-Pal;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.3
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    • pp.211-216
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    • 1986
  • To establish a suitability grouping system of paddy soils for double or multiple cropping with rice which is intensively practiced in the southern parts of Korea, a few basic experiments were carried out for two years. The results are summarized as follows; 1. The potential productivities of the paddy soils which were tested without any fertilizer in the pots of subsoil samples by the double cropping of rice and other upland crops were resulted that the soils of "Moderately well drained" fine silty textured were the highest while the soils of "Poorly drained" sandy were the lowest, and the productivities could be clearly comparable according to the differences of soil conditions. 2. The decomposability of organic matter also was higher in the soils of "Moderately well drained" than the "Imperfectly drained". The coarse loamy and coarse silty textured soils were high in the upland condition and in the early stages of submerging while the fine loamy and fine silty textured soils were high at the late stage of submerging in the rates of organic matter decomposition. 3. The days to be reached to tillable condition after rainfall in fine loamy textured soils were about 5 days earlier than the clayey soils. The period of tillable condition of fine clayey soils with "Moderately well drained" was the longest and that of the fine loamy textured soils was the shortest. But the soils with "Imperfectly drained" were not clear among soil textural classes. 4. The lower the ground water table the higher was the productivity indices. The variation of ground water table in the medium textured soils was higher than the both of coarse and fine textured soils among "Moderately well drained". But it was observed the opposite in the soils of "Imperfectly drained".

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Studies on the Organic Tiers Contained paddy Soils in Honam Area -II. Studies on the physicochemical characteristics of Organic Tiers and bearing power of soils (유기질토층(有機質土層)을 함유(含有)한 호남지역(湖南地域) 답토양(畓土壤)에 관(關)한 연구(硏究) -II. 유기질토층(有機質土層)의 이화학적(理化學的) 특성(特性) 및 토양(土壤)의 지지력(支持力))

  • Yoo, Chul-Hyun;Cho, Guk-Hyun;Choi, Jeong-Weon;Kim, Han-Myoung;Park, Keon-Ho;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.3
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    • pp.187-194
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    • 1986
  • Present stadies were carried out to obtain fundermental data for effective management of the soils by investigating the physicochemical characteristics rubber fiber, humification grade and bearing power of peats and muck which were included in Geongdeog series, and Gimje series in Honam area. The results abtained were as follows; 1. Humification grade of peats were about 23, while that of muck soils was about 45-71 and those were higher in Gimje series than Bongnam series. 2. The organic tiers which humification grade was higher were high pH, bulk density, and contents of K, $P_2O_5$, B.S and while were low OM, T-C, T-N, Na, $SiO_2$ and rubbed fiber. 3. In peats and mucks, bulk density, pH, contents of T-N $P_2O_5$ and ash were negatively correlated with rubbed fiber and OM, CEC, T-C, PAC, C/N, C/P were positively correlated with one, while these physicochemical characteristics were oppositely correlated with humification grade. 4. Cone bearing power of each soil tiers was low in every Geongdeog series which had the thick organic tiers and showed poorly drainage and it was the highest in Gimje series which was similar to in organic soils because of its thin organic tiers.

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Studies on the Soil Properties and Fertilizer Recommendation for Grass Lands to be Established (산지초지(山地草地) 조성대상지(造成對象地) 토양특성(土壤特性)과 시비추천(施肥推薦))

  • Lee, Hyub-Sung;Hur, Bong-Koo;Yoon, Kwan-Hee;Son, Eung-Ryong;Um, Ki-Tae;Noh, Dae-Chul;Kim, Young-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.301-306
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    • 1989
  • For the agricultural utilization of Korean forest land resources, which constitutes most of country, the distribution of environment and soil physico-chemical properties of establishable grass lands were clarified. The surveyed data were analized for the reasonable utilization and management of establishable grass land. The results were as follows ; 1. About 50.2% of the grass lands to be established were located under the 200m in altitude. The higher the altitude was, the more the organic matter content. 2. Tall type grass species such as Miscanthus purpurascens, Purple eulalia, and Themeda Japonica covered 71.3% of the natural vegetation in the soils of grass land to be established. 3. The extent of sandy and clayey soils which might be limited in the establishment of grassland was only 3.3%, meanwhile majority of the soils were in the favourable condition for grassland that is about 94% have more than 20cm in available soil depth and about 60.5% of the soils have less than 10% of gravels and stones in the soil profiles. 4. The chemical properties of the prearranged grassland soils were worse than the established grasslands, especially in the content of available $P_2O_5$. 5. The amount of fertilizer recommended for meadow were 286kg of nitrogen per hectare, 271kg of phosphorus, 224kg of potassium and 2040kg of calcium per hectare, but for grazing land were 201, 204, 136 and 1920kg/ha respectively.

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Differences of Nutrient Input by Throughfall, Stemflow and Litterfall between Deciduous Forest and Larix kaempferi Plantation in Mt. Joonwang, Kangwon-do (강원도 중왕산 지역 낙엽활엽수림과 낙엽송 조림지에서 수관통과우와 수간류 및 낙엽낙지에 의한 양분 유입의 차이)

  • Jung, Mun-Ho;Lee, Don-Koo;Um, Tae-Won
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.2
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    • pp.136-144
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    • 2007
  • The objectives of this study were to compare nutrient input by throughfall, stemflow and litterfall and concentration of nutrient in soil water between deciduous forest stand and Larix kaempferi plantation at Mt. Joongwang, Pyongchang-gun, Gangwon-do. The amount of rainfall interception during study period in deciduous forest stand and L. kaempferi plantation was 12% and 36%, respectively. Concentrations of cation ($Na^+$, $Mg^{2+}$, $K^+$ and $Ca^{2+}$) in throughfall were not different, while concentration of $Cl^-$ in stemflow was higher in L. kaempferi plantation. The results indicated that annual nutrient inputs by rainfall with the exception of $Cl^-$ were significantly greater in deciduous forest stand. In soil water, concentrations of anion ($Cl^-$, $NO_3{^-}$ and $SO{_4}^{2-}$) in A-layer, and $Ca^{2+}$ and $Cl^-$ in B-layer were higher in L. kaempferi plantation. Litterfall input during study period was $2,589kg\;ha^{-1}$ in deciduous forest stand and $1,046kg\;ha^{-1}$ in L. kaempferi plantation. Concentration of N was higher in L. kaempferi plantation, while N input from litterfall was greater in the deciduous forest stand ($36.81kg\;ha^{-1}yr^{-1}$) than L. kaempferi plantation ($16.16kg\;ha^{-1}yr^{-1}$). $Na^+$, $Mg^{2+}$, $K^+$ and $Ca^{2+}$ in litterfall collected from deciduous forest stand were found to be higher than those from the L. kaempferi plantation. Also, input of those were greater in deciduous forest stand. Thus, total nutrient input by throughfall, stemflow and litterfall was greater in deciduous forest stand than L. kaempferi plantation, significantly.

Augustin und die Rhetorik (아우구스티누스와 수사학)

  • Hahn, Seok-whan
    • Journal of Korean Philosophical Society
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    • v.116
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    • pp.389-410
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    • 2010
  • Augustin wurde sozusagen von der Rhetorik zum Christentum bekehrt. Der einstmalige Rhetorikprofessor (bis 386) distanziert sich von seiner $fr{\ddot{u}}heren$ Kunst. Aber er $kn{\ddot{u}}pft$ als Bischof im vierten Buch seiner weniger bekannten Schrift "De doctrina Christiana" (DDC; abgef. 427) wieder an die antike Rhetorik, speziell an Cicero, an. So wird die augustinische $Sp{\ddot{a}}tschrift$ $f{\ddot{u}}r$ eine antike Rhetorik in christlicher Verwendung gehalten. Es stellt nun die Frage, was Augustin zur $R{\ddot{u}}ckkehr$ zu seiner $fr{\ddot{u}}heren$ Kunst bewegte. Neuere Forschungen sehen in Augustins Werk eine Grundlage der mittelalterlichen Predigttheorie oder einen $blo{\ss}en$ Versuch, die Rhetorik vom Makel des Sophistischen zu befreien. Gewiss ist seine Einstellung zur "leeren Beredsamkeit" der Sophistik eindeutig, aber dies war eine Haltung, die letztlich von allen seinen christlichen Zeitgenossen geteilt wurde und folglich eines geringen Beweises bedurfte. Die Aufmerksamkeit, die Augustins $sp{\ddot{a}}terem$ Einfluss und seiner Ablehnung der Zweiten Sophistik geschenkt wird, kann den Blick $tr{\ddot{u}}ben$ $f{\ddot{u}}r$ seine Rolle bei der $L{\ddot{o}}sung$ eines christlichen Dilemmas aus dem vierten Jahrhundert. Augustin sah die Gefahren einer entgegengesetzten rhetorischen $H{\ddot{a}}resie$. Die $S{\ddot{u}}nde$ des Sophisten besteht darin, dass er die Notwendigkeit des Inhalts verneint und glaubt, nur die forma alleine sei $w{\ddot{u}}nschenswert$. Der gegenteilige Fehler, dem Geschichtsschreiber der Rhetorik niemals einen Namen gegeben haben, beruht auf dem Glauben, dass derjenige, der im Besitz der Wahrheit ist, auch ipso facto in der Lage ist, die Wahrheit anderen zu ${\ddot{u}}bermitteln$. Es handelt sich um eine $ausschlie{\ss}liche$ $Abh{\ddot{a}}ngigkeit$ von der materia. Augustin erkannte eine Gefahr und benutzte DDC dazu, eine Verbindung von Inhalt und Form in der christlichen Predigt voranzutreiben. Nur wenn man daher das Buch als einen Teil der $gro{\ss}en$ Debatte des vierten Jahrhunderts ansieht, tritt seine historische Bedeutung klar hervor. Der Leser ist beeindruckt davon, dass der Autor darauf insistiert, es sei eine Torheit, dem Feind ein $n{\ddot{u}}tzliches$ Instrument zu ${\ddot{u}}berlassen$. Augustin $erkl{\ddot{a}}rt$, dass die Kunst der Beredsamkeit rege in Gebrauch genommen und nicht kurzerhand abgelehnt werden solle, weil sie mit dem Makel des Heidentums behaftet sei. Kurz gesagt, geplant ist das vierte Buch von DDC als eine ratio eloquentiae Christianae.

Zwei Perspektiven für die Kunst - Kants Ästhethik des Empfangenden und Nietzsches Physiologie der Kunst - (예술에 대한 두 가지 태도 - 칸트의 수용미학과 니체의 예술생리학 -)

  • Chung, Nak-rim
    • Journal of Korean Philosophical Society
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    • v.130
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    • pp.277-304
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    • 2014
  • Der vorliegende Beitrag zielt darauft ab, zwei Perspektiven $f{\ddot{u}}r$ die Kunst bei Kant und Nietzsche und ihre Schlussfolgerungen zu $er{\ddot{o}}rtern$. Kants Kritik der Urteilskraft hat eine enorme Rolle in der Geschichte der ${\ddot{A}}sthetik$ gespielt. $F{\ddot{u}}r$ Kant sollen ${\ddot{a}}sthetische$ Urteile ebenso wie Erkenntnis- und Moralurteile $allgemeing{\ddot{u}}ltig$ sein, obwohl sie auf einem $pers{\ddot{o}}nlichen$ Geschmack beruhen. Die $Allgemeing{\ddot{u}}ltigkeit$ des $Sch{\ddot{o}}nen$ sei $m{\ddot{o}}glich$, weil sie nicht auf dem Gegenstand, sondern auf dem transzendentalen Subjekt basiere. Die $sch{\ddot{o}}ne$ Kunst als Kunst des Genies soll uns wie die $Natursch{\ddot{o}}nheit$ ohne Interesse wohlgefallen. Nietzsches Stellungnahme zu Kants ${\ddot{A}}sthetik$ ist sehr kritisch. Nietzsches erster Kritikpunkt richtet sich gegen das 'interesselose Wohlgefallen'. Gegen Kant behauptet Nietzsche, dass die $Sch{\ddot{o}}nheit$ sehr wohl mit Interesse verbunden ist. Grund $daf{\ddot{u}}r$ ist, dass das $Sch{\ddot{o}}ne$ wesentlich aus dem Willen zur Macht entspringt. Der zweite Kritikpunk Nietzsches liegt darin, dass in Kants ${\ddot{A}}sthetik$ die Moral im Vordergrund steht. Das $Sch{\ddot{o}}ne$ ist $f{\ddot{u}}r$ Kant durch die Moral gerechtfertigt. Nietzsche dreht diese Stellung der Moral zur Kunst um. Der dritte Kritikpunkt Nietzsches ist, dass Kant statt von der Erfahrung des $K{\ddot{u}}nstlers$ (Schaffenden) aus das ${\ddot{a}}sthetische$ Problem zu betrachten, allein vom Zuschauer (Empfangenden) aus ${\ddot{u}}ber$ die Kunst und das $Sch{\ddot{o}}ne$ nachgedacht habe. $F{\ddot{u}}r$ Nietzsche ist die Kunst $prim{\ddot{a}}r$ vom $K{\ddot{u}}nstler$ aus zu verstehen. Nietzsches Physiologie der Kunst ist mit dem Begriff 'Leib' $verkn{\ddot{u}}pft$, d.h. Nietzsche behauptet, dass physiologische und ${\ddot{a}}sthetische$ Prozesse wesentlich $zusammenh{\ddot{a}}ngen$. Die Schlussfolgerung der Physiologie der Kunst lautet: erstens, jeder Mensch ist $K{\ddot{u}}nstler$, sofern er schaffend ist, und zweitens, die Welt selbst ist nichts als Kunst. Nietzsches Physiologie der Kunst hat einen $gro{\ss}en$ Einfluss auf die $gegenw{\ddot{a}}rtige$ Kunst $ausge{\ddot{u}}bt$ und kein anderer Philosoph hat auf die $gegenw{\ddot{a}}rtige$ Kunst nachaltiger gewirkt.

Cytotoxicity of Particulate Matter in Various Human Cells Lines (미세먼지가 다양한 사람 세포주에 미치는 세포 독성)

  • Lee, Ji-Hyeon;Lee, Joo-Yeong;Kim, Mi-Jeong;Kim, Hyeon-Ji;Lee, Sung-Ho;Jeon, Byeong-Gyun
    • Journal of Life Science
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    • v.29 no.6
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    • pp.724-734
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    • 2019
  • The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.