• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.465-474
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• 2005

A two-step broad-range PCR method detecting gram-negative bacteria at the level as low as 2 CFU was developed by using primers of GNFI and GNRI and then semi-nested primer of GNF2 and GNRI. The nucleotide sequences of the primers were determined based on l6S rRNA gene. The DNA fragments of 1173 bp and 169 bp were amplified in one-step PCRs with primer sets of GNFI-GNRI and GNF2-GNRl, respectively, using template DNA from seven strains of gram-negative bacteria including Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas spp., and Acinetobacter baumaii but not from Achromobacter lyticus, Alca/igens faecalis, and five strains of gram-positive bacteria. DNA fragments of 180 bp were amplified from LTLT-pasteurized milk and UHf-pasteurized milk in the two-step PCR. The DNA fragments were amplified from LTLT-pasteurized milk which was added with Pseudomonas j/uorescens and subsequently heated at 65 $^{\circ}C$, 80 $^{\circ}C$, and 100 $^{\circ}C$ for 30 min but they were not amplified from the milk autoclaved at 121$^{\circ}C$ for 15 min. It was suggested in PCR that Pseudomonas fluorescens heated at 65 $^{\circ}C$ for 30 min in milk was more sensitive to DNase treatment than viable bacteria.

• Journal of Korea Multimedia Society
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• v.15 no.3
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• pp.364-371
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• 2012

Currently, CMMB(China Mobile Multimedia Broadcasting) and the conventional analog TV broadcasting have transmitted by using UHF(Ultra High Frequency : 474MHz~754MHz) band. Normally, the transmission power of the digital TV broadcasting is lower than the conventional analog TV broadcasting to protect the reception quality of the conventional analog TV broadcasting. The reception sensitivity of CMMB receiver has severely deteriorated due to adjacent the conventional analog TV broadcasting signals which called ACI (Adjacent Channel Interference). To improve the reception sensitivity of a CMMB receiver on ACI environment, this paper proposed a simple method which is tuning a cut off frequency of LPF (Low Pass Filter). From the experiment, the reception sensitivity of CMMB receiver was improved as 11.3dB.

• International Journal of Aeronautical and Space Sciences
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• v.15 no.3
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• pp.309-319
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• 2014

This paper mainly focuses on the conceptual design and stealth performance of the three-surface military aircraft. A three-dimensional (3-D) digital mock-up of the three-surface strike fighter with stealth feature was designed and the schemes of carrying missiles were analyzed in CATIA. Based on physical optics principle and the Method of Equivalent Currents (MEC), a numerical simulation of the RCS feature of the aircraft was carried out with RCSPlus which is a software designed by Beihang University. The paper contributes to the RCS feature analysis of the whole plane and different parts on X-band, S-band and UHF-band and a comparison of RCS feature to Su-37 and T-50 military aircraft is drawn. On X-band, the pitch angle of the incident wave was $0^{\circ}$, and the result shows: (1) Compared with Su-37 aircraft, the forward scattering RCS of the three-surface strike aircraft was reduced to 14.9%, the side scattering RCS to 9.6% and the back scattering RCS to 40.2%. (2) Compared with T-50 aircraft, the forward scattering RCS was reduced to 38.61%, and the side scattering RCS to 67.26%. This paper should be useful for researchers in conceptual design and stealth technology of the military aircraft.

• Journal of Magnetics
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• v.20 no.3
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• pp.308-311
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• 2015

We design a crisscrossed double-layer birdcage (DLBC) coil by modifying the coil geometry of a standard single-layer BC (SLBC) coil to enhance the homogeneity of transmitting magnetic flux density ($B_1{^+}$) along the main magnetic field ($B_0$)-direction for small-animal magnetic resonance imaging (MRI) at 300 MHz. The performance assessment of the crisscrossed DLBC coil is conducted by computational analysis with the finite-difference time domain method (FDTD) and compared with SLBC coil in terms of the $B_1$ and the $B_1{^+}$ distribution. As per the computational calculation studies, the mean value in the two-dimensional $B_1{^+}$ map obtained at the mid-axial slice with the proposed DLBC coil is slightly lower than that obtained with the SLBC coil, but the $B_1{^+}$ value of the DLBC coil in the outermost plane (40 mm away from the central plane) shows improvements of 19.3% and 24.8% over the SLBC coil $B_1{^+}$ value when simulating a spherical phantom and realistic mouse body modeling. These simulation results indicate that, the $B_1{^+}$ homogeneity along the z-direction was improved by using DLBC configuration. Our approach enables $B_1{^+}$ homogeneity improvement along the zdirection, and it can also be applied to ultra-high field (UHF) MRI systems.

• Proceedings of the KIEE Conference
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• 2002.07c
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• pp.1668-1670
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• 2002

Since more than two decades, the conventional PD detecting systems have been employed in order to detect the partial discharges occurring inside the HV power apparatus for their diagnosis by use of different type of detection such as acoustic and UHF detection method. Regardless of their wide on-site application, a certain number of technical inconveniences have been disclosed as follows : multistage amplification. large volume, susceptible to external noise and high price. In this respect, the optical measurement techniques are widely proposed in these days in this concerned field ascribed to the following advantages : immune to external EMI noise and broad band response of the Pockels cell covering from DC to GHz. However, the reliability of several proposed techniques enabling to measure the electric field inside the large high power apparatus has not yet been well approved In this work, an optical measuring system, based on the Pockels effect, has been developed for measuring the field variation due to the corona discharges occurring in air and in oil. This system consists of He-Ne laser, single mode optical fiber, multi mode optical fiber, polarizing film, Y-cut LiNbO3 cell, photo detector, digital oscilloscope and personal computer with GPIB. For this purpose, optical probe has been specially designed and realized and put into the needle-plane electrode. Afterward, same measurement is carried out in oil. We demonstrate the characteristic of the optical measuring system and the measurement results.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.2A
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• pp.177-183
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• 2009

In this paper, we propose an improved anti-collision algorithm. We have designed an anti-collision unit using this algorithm for the 18000-6 Type C Class 1 Generation 2 standard (Gen2). The Gen2 standard uses a Q-algorithm for incremental method on the Dynamic Slot-Aloha algorithm. It has basically enhanced performance over the Slot-Aloha algorithm. Unfortunately, there are several non-clarified parts: initial $Q_{fp}$ value, weighted C, and the ending point of the algorithm. If an incorrect value is selected, it causes degradation in performance. Thus we propose an improved anti-collision algorithm by clearly defining the vague parts of the existing algorithm. Simulation results showed an improved performance of up to 34.8% using an optimized value of C and the initial $Q_{fp}$ value. With the ending condition, performance is 34.7%. The anti-collision unit is designed using the Verilog HDL. The module was synthesized using Synopsys' Design Compiler and the TSMC $0.2{\mu}m$ standard cell library. The synthesized result yielded 3,847 gates, and was guaranteed under the proposed working frequency of 19.2MHz.