• 한국측량학회지
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• 제36권6호
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• pp.457-467
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• 2018

공공측량/통합기준점측량 작업규정은 기존 트래버스 측량 작업규정을 준용하고 있으며, GNSS관측 특성을 정확하게 반영하지 않아서 현장 작업과 자료처리에 어려움이 있다. 또한, GNSS관측 자료처리 절차에 대한 규정이 명확하지 않고, 정확도 검증방법 역시 일반적인 기준과 차이가 있다. 본 연구에서는 현재 규정을 분석하고 적절한 업무프로세스를 제안하기 위해 공공기준점 측량과 유사한 시나라오를 바탕으로 짧은 세션(30분) 데이터를 처리했다. 서울특별시 네트워크 RTK (Real Time Kinematic) 기준점에 대해서 3일간 동일한 시간대 결과를 비교했으며, 하루 중 시간에 따른 결과를 비교해서 전반적인 자료처리 정확도를 평가했다. 대류권 지연오차 추정여부에 따른 정확도 차이를 동시에 분석했으며, 추정결과는 24시간 정지측량 결과와 비교했다. 대류층 지연오차를 추정하는 것이 정확도와 좌표안정성 향상에 유리하며, 평균제곱근오차는 대략 평면 5mm, 수직 1cm 수준으로 추정되었다. 본 연구결과를 바탕으로 통합기준점을 포함한 동시관측 일간해를 추정하고, 이를 통합하여 최소제약조건을 통해 최종해를 결정하는 업무프로세스를 제안한다. 이를 위해서는 학술용 자료처리시스템을 이용한 자료처리자동화시스템이 구축되어야하며, GNSS자료처리를 위해 통합기준점과 공공기준점 코드를 표준화해야 한다.

• 한국압력기기공학회 논문집
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• 제12권1호
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• pp.11-16
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• 2016

Fuel assembly's mechanical characterization test facility (FAMeCT) in KAERI was constructed with upgraded functional features such as increased loading capacity, underwater vibration testing and severe earthquake simulation for extended fuel design guideline. This facility is designed and developed to provide out-pile fuel data for accident analysis model and fuel licensing. Functional tests of FAMeCT were performed to confirm functionality, structural integrity, and validity of newly-built fuel assembly mechanical test facility. Test program includes signal check of data acquisition system, load delivering capacity using real-sized fuel assemblies and a standard loading cylindrical rigid specimen. Fuel assembly's lateral bending test was carried out up to 30 mm of pull-out displacement. Limit case axial compression loading test up to 33 kN was performed to check structural integrity of UCPS (Upper Core Plate Simulator) support frame. Test results show that all test equipment and measurement system have acceptable range of alignment, signal to noise ratio, load carrying capacity limit without loss of integrity. This paper introduces newly constructed fuel assembly's mechanical test facility and summarizes results of functional test for the mechanical test equipment and data acquisition system.

• Molecules and Cells
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• 제39권9호
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• pp.680-686
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• 2016

Uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that function to dissipate proton motive force and mitochondrial membrane potential. One UCP has been identified in Caenorhabditis elegans (C. elegans), namely UCP-4. In this study, we examined its expression and localization using a GFP marker in C. elegans. ucp-4 was expressed throughout the body from early embryo to aged adult and UCP-4 was localized in the mitochondria. It is known that increased mitochondrial membrane protential leads to a reactive oxygen species (ROS) increase, which is associated with age-related diseases, including neurodegenerative diseases in humans. A ucp-4 mutant showed increased mitochondrial membrane protential in association with increased neuronal defects during aging, and the neurons of ucp-4 overexpressing animals showed decreased neuronal defects during aging. These results suggest that UCP-4 may be involved in neuroprotection during aging via relieving mitochondrial membrane protential. We also investigated the relationship between UCP-4 and innate immunity because increased ROS can affect innate immunity. ucp-4 mutant displayed increased resistance to the pathogen Staphylococcus aureus compared to wild type. The enhanced immunity in the ucp-4 mutant could be related to increased mitochondrial membrane protential, presumably followed by increased ROS. In summary, UCP-4 might have an important role in neuronal aging and innate immune responses through mediating mitochondrial membrane protential.

• Nutritional Sciences
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• 제6권3호
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• pp.148-154
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• 2003

Although various raw plant materials have been demonstrated to exert anti-obesity effects to a greater or lesser extent in both humans and animals when they are used to supplement the diet, it has not been shown extensively that they influence adipocyte cell differentiation involving lipid metabolic gene expressions. Using a well-established 3T3-L1 preadipocyte differentiation system, we decided to look into molecular and cellular event occurring during adipocyte differentiation when raw plant materials aye included in the process, in an effort to demonstrate the potential use of a screening system to define the functions of traditionally well-known materials. To these ends, the effects of ethanol (EtOH) or EtOH/distilled water (DW) extracts of Wax Gourd were examined using cytochemical and molecular analyses to determine whether components of the extracts modulate adipocyte differentiation of 3T3-Ll preadipocytes in vitro. The cytochemical results demonstrated that EtOH or EtOH/DW extracts did not affect lipid accumulation and cell proliferation, although the degree of lipid accumulation was influenced slightly depending on the extract. EtOH extract was highly effective in apoptotic induction during differentiation of 3T3-Ll preadipocytes (p<0.05). Reverse transcription-polymerase chain reaction (RT-PCR) analysis of lipoprotein lipase (LPL), Uncoupling protein (Ucp) 2, 3 and 4 also showed that while LPL expression was not influenced, Ucp2, 3 and 4 were up regulated in the EtOH extract-treated group and down regulated in the EtOH/DW extract-treated group. These changes in gene expressions suggest that the components in different fractions of Wax Gourd extracts may modulate lipid metabolism by either direct or indirect action. Taking these results together, it was concluded that molecular and cellular analyses of adipocyte differentiation involving lipid metabolic genes should facilitate understanding of cellular events occurring during adipocyte differentiation. Furthermore, the experimental scheme and analytical methods used in this study should provide a screening system for the functional study of raw plant materials in obesity research.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1552-1556
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• 2005

The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.

• 한국측량학회지
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• 제36권5호
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• pp.403-412
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• 2018

Recently, GNSS (Global Navigation Satellite System)-derived orthometric height determination has been studied to improve the time and cost-effectiveness of traditional leveling surveying. However, the accuracy of this new survey method was evaluated when unknown points are located lower than control points. In this study, the accuracy of GNSS-derived orthometric height was examined using TPs (Triangulation Points) to verify the stability of surveying in mountainous areas. The GNSS survey data were obtained from Mungyeong, Unbong/Hadong, Uljin, and Jangseong. Three unknown points were surrounded by more than three UCPs (Unified Control Points) or BMs (Benchmarks) following the guideline for applying GNSS-derived orthometric height determination. A newly developed national geoid model, KNGeoid17 (Korean National Geoid 2017), has been applied for determining the orthometric height. In comparison with the official orthometric heights of the TPs, the heights of the unknown points in Mungyeong and Unbong/Hadong differ by more than 20 cm. On the other hand, TPs in Uljin and Jangseong show 15-16 cm of local bias with respect to the official products. Since the precision of official orthometric heights of TPs is known to be about 10 cm, these errors exceed the limit of the precision. Therefore, the official products should be checked to offer more reliable results to surveyors. As an alternative method of verifying accuracy, three different GNSS post-processing software were applied, and the results from each software were compared. The results showed that the differences in the whole test areas did not exceed 5 cm. Therefore, it was concluded that the precision of the GNSS-derived orthometric height was less than 5 cm, even though the unknown points were higher than the control points.

• 한국측량학회지
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• 제34권1호
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• pp.91-98
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• 2016

지구통계학적인 공간분석의 대표적인 방법인 크리깅(kriging)을 적용하기 위해서는 두 관측점 사이의 거리에 기반한 상관성을 나타내는 공간상관함수의 추정이 우선적으로 이루어져야 한다. 본 연구에서는 다양한 크리깅에 적용할 수 있는 대표적인 상관함수인 semi-variogram, homeogram, covariance function에 대하여 국가지오이드 모델을 기반으로 추정하였다. 경위도 각각 2°의 대상지역 내 통합기준점의 지오이드고를 이용하였으며, 선형모델을 이용하여 공간적인 편향성을 제거하였다. 전체 100개의 샘플 포인트에 대해서 중복되지 않은 두 점 간의 거리를 기준으로 구간을 나누고, 각 함수에 대한 경험적인 값을 계산하였다. 공간상관함수의 경험적인 값은 각각 두 개의 모델에 최소제곱조정 방법으로 피팅한 결과 semi-variogram의 wave 모델 적합도가 가장 높았으며, homeogram과 covariance function은 exponential 모델이 상대적으로 좋은 피팅 결과를 보였다. 본 연구에서 결정한 공간상관함수는 추후 다양한 크리깅 방법을 통해 임의 지점에서의 예측값에 대한 정확도 검증과 이에 대한 평균제곱예측오차(Mean Squared Prediction Error, MSPE)를 계산함으로써 각 함수의 활용성에 대한 추가적인 연구가 수행되어야 한다.

• 대한공간정보학회지
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• 제24권2호
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• pp.47-54
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• 2016

This study aims to analyze the accuracy for feasibility study of Network-RTK(VRS) surveying inside and outside of Korean CORS network. The southwest coast of Korea where some part of mainland and islands are outside of CORS network is chosen as the test area. To evaluate the accuracy of VRS surveying at surveying points, several Unified Control Points (UCPs) inside and outside of Korean CORS network were selected as the points in the test area. The feasibility of VRS surveying was analyzed by investigating the errors related to the location of points inside and outside of CORS network and the difference of 3-dimensional coordinates observed on different date. As the results of this study, the orthometric height errors of points outside of CORS network based on KNGeoid14 were improved about 5.0 cm in RMSE in comparison with KNGeoid13. The height errors of VRS surveying were considered to be less relevant to the results from PDOP and number of satellites (GPS and GLONASS). However, the orthometric errors caused by the geoidal height of KNGeoid and the ellipsoidal height of VRS surveying at points located outside of CORS network need to be addressed carefully for control surveying. When a point surveyed twice on different date, the difference of the ellipsoidal height of the point outside of CORS network was larger than that of the point inside of CORS network.

• 한국식품과학회지
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• 제48권4호
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• pp.384-391
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• 2016

선행연구(12,13)에 따르면 10주간 ID1216을 투여한 비만 마우스에서 체중과 체지방이 감소하였고 이는 SIRT1-$PGC1{\alpha}$의 발현을 조절하여 나타나는 것으로 확인하였다. 본 연구는 $SIRT1-PGC1{\alpha}-PPAR{\alpha}$의 하위 기전인 UCPs, ACO, aP2의 발현 조절에 ID1216이 영향을 미쳐 그 효과를 나타내는 것을 추가로 확인한 것에 의미가 있다. 또한 10주간 ID1216을 투여한 비만 마우스의 혈액 분석 결과에서도 혈중 중성지방, LDL, HDL total cholesterol등의 혈중 지방질 수치가 개선됨과 동시에 free fatty acid의 농도는 감소하였는데 이는 ID1216이 HSL과 같은 지방질분해효소의 활성을 조절하여 중성지방의 분해과정에 관여하기는 하나 에너지 대사와 지방산 산화 과정에도 복합적으로 관여하여 최종적으로 나타내는 비만 대사 조절 효과에 의한 것으로 판단된다. 따라서 ID1216은 $SIRT1-PGC1{\alpha}-PPAR{\alpha}$ pathway를 촉진시켜 세포와 조직 수준에서 열발생(thermogenesis)에 관여하는 유전자인 UCP1, UCP2, UCP3의 발현을 증가시켰고 ${\beta}$-oxidation에 관여하는 유전자인 ACO와 aP2의 발현도 증가시켰으며 또한 지방분해(lypolysis)에 관여하는 유전자인 ATGL과 HSL의 발현을 증가시키는 분자생물학적 기전을 나타내어 체지방 감소 효과를 나타내는 것으로 확인되었다.

• 대한본초학회지
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• 제27권2호
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• pp.53-59
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• 2012

Objectives : This study was undertaken to investigate the effects of the GGEx18 ethyl acetate fraction (EF) on lipid accumulation and gene expression of fatty acid-oxidizing enzymes using 3T3-L1 adipocytes, C2C12 skeletal muscle cells, and NMu2Li liver cells. Methods : PPAR${\alpha}$, AMPK and UCPs transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Compared with control, EF significantly increased the mRNA expression of VLCAD in 3T3-L1 adipocytes. 2. Compared with control, EF (0.1 ${\mu}g/ml$) significantly inhibited lipid accumulation in 3T3-L1 adipocytes. 3. EF significantly increased the mRNA expression of AMPK${\alpha}$1, AMPK${\alpha}$2 and PPAR${\alpha}$ in C2C12 skeletal muscle cells compared with control. 4. EF significantly increased the mRNA expression of genes involved in fatty acid ${\beta}$-oxidation, such as thiolase, MCAD, and CPT-1 in C2C12 skeletal muscle cells compared with control. 5. EF significantly increased the mRNA expression of UCP2 involved in energy expenditure in C2C12 skeletal muscle cells compared with control. 6. Compared with control, EF (10 ${\mu}g/ml$) significantly inhibited lipid accumulation in C2C12 skeletal muscle cells. 7. EF (10 ${\mu}g/ml$) significantly increased the mRNA expression of ACOX, HD, VLCAD and MCAD in NMu2Li liver cells compared with control. Conclusions : These results suggest that EF may prevent obesity by increasing the mRNA expression of mitochondrial fatty acid ${\beta}$-oxidizing enzymes in 3T3-L1 adipocytes, by not only regulating the fatty acid oxidation through activation of AMPK and PPAR${\alpha}$, but also increasing the UCP2 mRNA expression in C2C12 skeletal muscle cells, and by stimulating the mRNA expression of fatty acid-oxidizing enzymes in NMu2Li liver cells.