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http://dx.doi.org/10.6116/kjh.2012.27.2.53

Ethyl acetate fraction of GGEx18 modulates fatty acid β-oxidizing enzymes  

Joo, Byung-Soo (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
Lee, Hee-Young (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
Lee, Hye-Rim (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
Yoon, Mi-Chung (Dept. of Life Sciences, Mok-Won University)
Seo, Bu-Il (Dept. of Herbology, College of Oriental Medicine, Daegu Haany University)
Kim, Beom-Hoi (Dept. of Anatomy, College of Oriental Medicine & Research Institute of Oriental Medicine, Dong-Eui University)
Shin, Soon-Shik (Dept. of Formula Science, College of Oriental Medicine, Dong-Eui University)
Publication Information
The Korea Journal of Herbology / v.27, no.2, 2012 , pp. 53-59 More about this Journal
Abstract
Objectives : This study was undertaken to investigate the effects of the GGEx18 ethyl acetate fraction (EF) on lipid accumulation and gene expression of fatty acid-oxidizing enzymes using 3T3-L1 adipocytes, C2C12 skeletal muscle cells, and NMu2Li liver cells. Methods : PPAR${\alpha}$, AMPK and UCPs transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Compared with control, EF significantly increased the mRNA expression of VLCAD in 3T3-L1 adipocytes. 2. Compared with control, EF (0.1 ${\mu}g/ml$) significantly inhibited lipid accumulation in 3T3-L1 adipocytes. 3. EF significantly increased the mRNA expression of AMPK${\alpha}$1, AMPK${\alpha}$2 and PPAR${\alpha}$ in C2C12 skeletal muscle cells compared with control. 4. EF significantly increased the mRNA expression of genes involved in fatty acid ${\beta}$-oxidation, such as thiolase, MCAD, and CPT-1 in C2C12 skeletal muscle cells compared with control. 5. EF significantly increased the mRNA expression of UCP2 involved in energy expenditure in C2C12 skeletal muscle cells compared with control. 6. Compared with control, EF (10 ${\mu}g/ml$) significantly inhibited lipid accumulation in C2C12 skeletal muscle cells. 7. EF (10 ${\mu}g/ml$) significantly increased the mRNA expression of ACOX, HD, VLCAD and MCAD in NMu2Li liver cells compared with control. Conclusions : These results suggest that EF may prevent obesity by increasing the mRNA expression of mitochondrial fatty acid ${\beta}$-oxidizing enzymes in 3T3-L1 adipocytes, by not only regulating the fatty acid oxidation through activation of AMPK and PPAR${\alpha}$, but also increasing the UCP2 mRNA expression in C2C12 skeletal muscle cells, and by stimulating the mRNA expression of fatty acid-oxidizing enzymes in NMu2Li liver cells.
Keywords
Gyeongshingangjeehwan18; ethyl acetate fraction; fatty acid ${\beta}$-oxidation; obesity;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Ki Jeong Park. Ethyl acetate fraction of GGEx18 modulates feeding efficiency ratio and blood leptin level in high fat diet-fed obese mice. thesis for the degree of master of oriental medicine, the graduate school, Dongeui University, 2011:8-32.
2 Couillard C, Mauriege P, Imbeault P, Prud'homme D, Nadeau A, Tremblay A, Bouchard C, Després JP. Hyperleptinemia is more closely associated with adipose cell hypertrophy than with adipose tissue hyperplasia. Int J Obes Relat Metab Disord 2000; 24:782-8.   DOI   ScienceOn
3 Suzuki A, Okamoto S, Lee S, Saito K, Shiuchi T, Minokoshi Y. Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase. Mol Cell Biol. 2007;27(12):4317-27.   DOI   ScienceOn
4 Minokoshi Y, Kim YB, Peroni OD, Fryer LG, Müller C, Carling D, Kahn BB. Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase. Nature. 2002;415(6869):339-43.   DOI   ScienceOn
5 Lim CT, Kola B, Korbonits M. AMPK as a mediator of hormonal signalling. J Mol Endocrinol. 2010;44(2):87-97.   DOI   ScienceOn
6 Herbology Association of Korea's Colleges of Oriental Medicine. "Textbook of Herbology". First Edition. Seoul: younglim press, 2004:154-6, 285-7, 510-1.
7 Clapham JC, Arch JR, Chapman H, Haynes A, Lister C, Moore GB, Piercy V, Carter SA, Lehner I, Smith SA, Beeley LJ, Godden RJ, Herrity N, Skehel M, Changani KK, Hockings PD, Reid DG, Squires SM, Hatcher J, Trail B, Latcham J, Rastan S, Harper AJ, Cadenas S, Buckingham JA, Brand MD, Abuin A. Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean. Nature 2000;406:415-8.   DOI   ScienceOn
8 Yoon M, Jeong S, Lee H, Han M, Kang J-H, Kim EY, Kim M, Oh GT. Fenofibrate improves lipid metabolism and obesity in ovariectomized LDL receptor-null mice.Biochem Biophys Res Commun. 2003;302:29-34.   DOI   ScienceOn
9 Jeong S, Kim M, Han M, Lee H, Ahn J, Kim M, Song Y-H, Shin C, Nam K-H, Kim T W, Oh G T, Yoon, M. Fenofibrate prevents obesity and hypertriglyceridemia in LDL receptor-null mice. Metabolism. 2004;53:607-13.   DOI   ScienceOn
10 Jeong S, Yoon M. Inhibition of the actions of peroxisome proliferator-activated receptor alpha on obesity by estrogen.. Obesity 2007;15: 1430-40.   DOI
11 Wang S, Subramaniam A, Cawthorne MA, Clapham JC. Increased fatty acid oxidation in transgenic mice overexpressing UCP3 in skeletal muscle. Diabetes Obes Metab 2003;5:295-301.   DOI   ScienceOn
12 Hesselink MK, Mensink M, Schrauwen P. Human uncoupling protein-3 and obesity:an update. Obes Res 2003;11:1429-43.   DOI   ScienceOn
13 Choi CS, Fillmore JJ, Kim JK, Liu ZX, Kim S, Collier EF, Kulkarni A, Distefano A, Hwang YJ, Kahn M, Chen Y, Yu C, Moore IK, Reznick RM, Higashimori T, Shulman GI. Overexpression of uncoupling protein 3 in skeletal muscle protects against fat-induced insulin resistance. J Clin Invest 2007;117:1995-2003.   DOI   ScienceOn
14 Costet P, Legendre C, More J, Edgar A, Galtier P, Pineau T. Peroxisome proliferator-activated receptor alpha-isoform deficiency leads to progressive dyslipidemia with sexually dimorphic obesity and steatosis. J Biol Chem. 1998;273: 29577-85.   DOI
15 Yoon M, Jeong S, Nicol CJ, Lee H, Han M, Kim J, Seo Y, Ryu C, Oh GT. Fenofibrate regulates obesity and lipid metabolism with sexual dimorphism. ExpMolMed. 2002;34:481-8.
16 Chaput E, Saladin R, Silvestre M, Edgar AD. Fenofibrate and rosiglitazone lower serum triglycerides with opposing effects on body weight. Biochem Biophys Res Commun 2000;271: 445-50.   DOI   ScienceOn
17 Guerre-Millo M, Gervois P, Raspe E, Madsen L, Poulain P, Derudas B, Herbert JM, Winegar DA, Willson TM, Fruchart JC, Berge RK, Staels B. Peroxisome proliferator-activated receptor alpha activators improve insulin sensitivity and reduce adiposity. J Biol Chem. 2000;275:16638-42.   DOI   ScienceOn
18 Mancini FP, Lanni A, Sabatino L, Moreno M, Giannino A, Contaldo F, Colantuoni V, Goglia F. Fenofibrate prevents and reduces body weight gain and adiposity in diet-induced obese rats. FEBS Lett. 2001;491:154-8.   DOI   ScienceOn
19 Xue Min Gao editor-in-chief. "Chinese Herbology Medicine"(part 1). First Edition. Beijing:People's Medical Publishing House, 2000:178, 595.
20 Xue Min Gao editor-in-chief. "Chinese Herbology Medicine"(part 2). First Edition. Beijing:People's Medical Publishing House, 2000:1246.
21 Ki Hyeon Yoon, Hee Young Lee, Yang Sam Jung, Bu-Il Seo, Gyu-Ryeol Park, Michung Yoon, Soon Shik Shin. Modulation of obesity by Gyeongshingangjeehwan18 in ob/ob mice. The Korea Journal of Herbology 2010;25(3):3-8.
22 Yoo In Yang. GGEx18 reduces body weight gain and increases PPAR alpha target gene in high fat diet induced obese mice. thesis for the degree of doctor of oriental medicine, the graduate school, Dongeui University, 2009:8-50.
23 Chang Min Cha. Modulation of GGEx18 by fatty acid $\beta$-oxidation in C2C12 skeletal muscle. thesis for the degree of master of oriental medicine, the graduate school, Dongeui University, 2009:9-21.