• Molecules and Cells
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• 제28권5호
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• pp.463-472
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• 2009

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of development in rice plants.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.599-602
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• 2005

Truncated form of UBP1, an ubiquitin-specific protease of Saccharomyces cerevisiae, was overexpressed in Escherichia coli. The hexahistidine residue $(His_6)$ was fused to the N-terminus of truncated UBP1 and the corresponding recombinant protein was purified with high yield by immobilized metal affinity chromatography. The truncated form of UBP1 protein was functional to cleave ubiquitinated human growth hormone as substrate. Effects of pH and temperature were investigated in order to optimize deubiquitinating reactions for the truncated UBP1. Optimum temperature and pH for the cleavage reaction were $40^{\circ}C$ and pH 8.0, respectively.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.554-556
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• 2003

본 연구에서는 고부가가치 의약단백질인 human growth hormone을 고순도로 얻기 위하여 재조합 대장균을 이용하여 ubiquitin이 융합된 형태로 단백질을 발현시키고, 이를 분해하는 ubiquitin-specific pretense를 발현시켜 이를 분리 ${\cdot}$ 정제하고 효소특성을 살펴보았다. UBP1 enzyme을 재조합 대장균을 이용하여 발현하고 분리 ${\cdot}$ 정제한 결과, 분자량은 약 83.5kDa이었으며, $40^{\circ}C$, pH 8.0에서 최대 효소활성을 보였다.

• International Journal of Control, Automation, and Systems
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• 제3권1호
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• pp.15-31
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• 2005

Nowadays, the road safety is an important research field. One of the principal research topics in this field is the vehicle localization in the road network. This article presents an approach of multi sensor fusion able to locate a vehicle with a decimeter precision. The different informations used in this method come from the following sensors: a low cost GPS, a numeric camera, an odometer and a steer angle sensor. Taking into account a complete model of errors on GPS data (bias on position and nonwhite errors) as well as the data provided by an original approach coupling a vision algorithm with a precise numerical map allow us to get this precision.

• 유기물자원화
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• 제16권2호
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• pp.81-87
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• 2008

본 연구에서는 버려지는 연탄재를 하수처리에 이용하여 그 처리효율 및 활용성 등을 조사하였다. 본 연탄재 공법에 의해 처리된 처리수의 $BOD_5$, CODcr, SS 등은 각각 1.1 (mg/L), 9.5 (mg/L), 3.8(mg/L)를 나타내며 원수와 비교하여 99%, 92.7%, 96.3%의 처리효율을 보여주었다. 본 연탄재공법에 의한 처리수의 총 세균 및 대장균에 대한 제거효율은 각각 평균 9 (cfu/ml) 및 1.2 (cfu/ml)로 나타나 기존의 하수처리장 방류수와 비교하여 괄목할만한 감소효과를 보여주었다. 한편, 1년 이상의 장기적 운전실험에서는 변함없는 높은 처리효율의 유지와 함께 계절별 처리효율의 변동폭이 매우 낮았으며, 슬러지발생이 차단되는 효과를 보여주었다. 본 연탄재공법은 연탄재를 재활용함은 물론, 높은 처리효율과 슬러지의 발생을 감소시킴으로써 환경친화적인 공법이라 할 수 있으며, 중소규모의 하수처리 및 처리수의 재활용에 매우 유용할 것으로 사료되는 바이다.

• 한국동물학회:뉴스레터
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• 제12권2호
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• pp.111.2-111
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• 1995

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.247.2-247
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• 1995

No Abstract, See Full Text

• 대한안전경영과학회지
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• 제14권2호
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• pp.147-158
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• 2012

This paper aims to develop a new chain metrics for obtaining lean Overall Equipment Effectiveness(OEE) and present implementation strategy which considers the properties for Total Productive Maintenance(TPM) to reduce machine losses, Performance Analysis and Control(PAC) to reduce labor losses, Lean Production System(LPS) to reduce floor wastes, and Theory of Constraints(TOC) to minimize the problem of Capacity Constrained Resource(CCR). The study reviews the related literatures and reformulates the structure of machine losses, labor losses and field wastes. The research also develops the integrated productivity metrics according to time, units, reliability and maintainability. It is found that the study develops the actual productivity measure in terms of efficiency, effectiveness and standard productivity. In addition to that, it outlines and develops by using the integrated LPS and TPM, lean OEE measures such as Time Based Productivity(TBP), Unit Based Productivity(UBP), and Reliability & Maintainability Based Availability(RMBA). Implication examples are proposed to make it easier and available for practioners to understand the implementation strategies about TPM OEE, lean OEE and TOC OEE. Futhermore related to other studies, the research contributes to create a new chain productivity measures to clear the interrelationship concepts of productivity, efficiency and effectiveness. Moreover the paper develops the enhanced OEE measures by integration of TPM, PAC, LPS and TOC with the perspective of schedule, throughput, reliability, maintainability and availability.

• 대한소아치과학회지
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• 제49권4호
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• pp.379-391
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• 2022

이 연구는 premixed MTA 제재와 기존 치수복조제의 경화 전, 후의 pH 값 그리고 칼슘, 황, 스트론튬 이온의 유리량, 용해도를 비교했다. 사용된 재료는 다음과 같다 : 레진 강화형 칼슘실리케이트(TheraCal LC^®; TLC), 레진 강화형 수산화칼슘(UBP, Ultra-Blend^TM plus), 2종류의 premixed MTA(Endocem MTA^® premixed regular [EMPR] and Well-Root^TM PT [WRP]). 각 재료의 시편은 경화 전, 경화 후 2군으로 나누어 준비한 뒤 증류수에 보관하였다. pH, 용해도를 측정하였으며 ICP-AES를 이용한 칼슘, 황, 스트론튬의 3가지 이온 유리량을 측정하였다. 경화 후 군에서 TLC와 UBP의 pH 값은 감소했다. 그러나 premixed MTA 재료의 pH 값은 증가했다. TLC는 다른 재료와 비교하여 스트론튬 이온 유리량이 더 많았다. 동시에 EMPR에서 황이온 유리량이 높았다(p < 0.05). 경화 후 군에서 칼슘 이온 방출은 두 종류의 premixed MTA에서 더 높았다(p < 0.05). 경화 후 군에서 용해도는 Kruskal-Wallis 서 test를 이용하여 통계분석하였고 Mann-Whitney U test를 이용하여 사후검정하였다. 결론적으로 레진 강화형 칼슘 실리케이트 시멘트, 레진 강화형 수산화칼슘 시멘트, 2종류의 premixed MTAs 모두 경화 후 알칼리성 pH 값과 낮은 용해도를 가지고 있었으며 다양한 이온을 유리했다.

• 한국정보처리학회논문지
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• 제6권11S호
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• pp.3350-3355
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• 1999

Modeling for the Internet resource is important to use of limited resource efficiently. Also, the modeling could guide how we can approach the Internet problems. The many studies of price mechanism to internalize the externality, to decrease the externality from congestion, and to use the Internet resource efficiently, are widely going on. The represent styles for Internet pricing are usage-based pricing(UBP) and flat-rat pricing(FRP) and the FRP could prefers for the beginning stage of Internet introduce. The purpose of this study is to examine the efficiency of Internet pricing mechanism based on flat-rate pricing, both consumer and producer side.