• Journal of Genetic Medicine
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• 제20권2호
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• pp.39-45
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• 2023

As advanced sequencing technologies continue to uncover an increasing number of variants in genes associated with human genetic diseases, there is a growing demand for systematic approaches to assess the impact of these variants on human development, health, and disease. While in silico analyses have provided valuable insights, it is essential to complement these findings with model organism studies to determine the functional consequences of genetic variants in vivo. Drosophila melanogaster is an excellent genetic model for such functional studies due to its efficient genetic technologies, high gene conservation with humans, accessibility to mutant fly resources, short life cycles, and cost-effectiveness. The traditional GAL4-UAS system, allowing precise control of gene expression through binary regulation, is frequently employed to assess the effects of monoallelic variants. Recombinase medicated cassette exchange or CRISPR-Cas9-mediated GAL4 insertion within coding introns or substitution of gene body with Kozak-Gal4 result in the loss-of-function of the target gene. This GAL4 insertion strategy also enables the expression of reference complementary DNA (cDNA) or cDNA carrying genetic variants under the control of endogenous regulatory cis elements. Furthermore, the CRISPR-Cas9-directed tissue-specific knockout and cDNA rescue system provides the flexibility to investigate candidate variants in a tissue-specific and/or developmental-timing dependent manner. In this review, we will delve into the diverse genetic techniques available in Drosophila and their applications in diagnosing and studying numerous undiagnosed diseases over the past decade.

• Animal cells and systems
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• 제6권3호
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• pp.271-275
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• 2002

Rph1 and Gisl are damage-responsive repressors involved in PHR1 expression. They have two $C_{2}$H/ sub 2/ zinc finger motifs as putative DNA binding domains and N-terminal conserved domain with unknown function. They are also found in the human retinoblastoma binding protein 2 and the mouse jumonji- encoded protein. The repressors are able to bind to A $G_{4}$ sequence within a 39-bp sequence called upstream repressing sequence of PHR1 promoter (UR $S_{PHR1}$) responsible for the damage-response of PHR1. We report here that Rph1 is predominantly localized in the nucleus as examined by fluorescence microscopic analysis with GFP-Rph1 fusion protein. On the basis of the fact that the A $G_{4}$ sequence that is recognized by Rph1 and Gisl is also recognized by Msn2 and Msn4 in a process of stress response, we a1so tried to examine the in vivo function of A $G_{4}$ and the role of Msn2 and Msn4 in PHR1 expression. Our results demonstrate that Msn2 and Msn4 are actually required for the basal transcription of PHR1 expression but not for its damage induction. When A $G_{4}$ sequence was inserted into the minimal promoter of the cyc1-LacZ reporter, the increased LacZ expression was observed indicating its involvement in transcriptional activation. The data suggest that the A $G_{4}$ is primarily required for basal transcriptional activation of PHR1 or CYC1 promoter through the possible involvement of Msn2 and Msn4. However, since the A $G_{4}$ is also involved in the repression of PHR1 via Rphl and Gisl, it is proposed that A $G_{4}$ functions as either URS or upstream activating sequence (UAS) depending on the promoter context.t.

• 대한토목학회논문집
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• 제34권5호
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• pp.1645-1653
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• 2014

대부분의 지형정보획득을 위한 영상에는 RGB, 근적외선, 열영상이 주로 사용된다. 이 멀티밴드영상은 위성이나 유인항공기에 탑재되어 획득되고 있으나 주기해상도, 비용, 공간해상도, 그리고 구름의 영향 등으로 사용자를 만족시키기 어렵다. 자동항법UAV에 적합한 페이로드와 콘트롤러를 개발한다면 원하는 시간과 주기로 고해상도 멀티밴드영상을 획득할 수 있다. 본 연구에서는 멀티밴드 영상획득을 위한 센서와 페이로드의 개발을 통해 저가의 고해상 영상획득시스템을 구축하고 이를 이용하여 geo-referencing data와 함께 RGB, NIR과 열영상을 획득하였다. 획득한 RGB영상으로 정사모자익영상을 제작하여 검사점에 대한 위치정확도를 분석한 결과 수평좌표에서 0.181m, 수직좌표에서 0.203m의 편차를 얻을 수 있었다. 이는 1:1,000~5000수치지도제작과 소규모지역에 대한 원격탐측이 가능한 공간정확도를 만족하므로 페이로드의 활용성을 검증할 수 있었으며 활용이 기대된다.

• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.175-182
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• 2002

Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

• 한국환경과학회지
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• 제12권7호
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• pp.715-724
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• 2003

Distributional patterns of microalgae were studied in the tidal flats of Gamami Beach(Young-Gwang, Korea) from November 1999 to 2000 July. The tidal flats of Gamami Beach was composed mainly of sandy sediment. The concentrations of nutrients were low compared with other tidal flats. In the present study, 68 species of microalgal flora were identified. These were comprised of 25 species of benthic microalgae and 59 species of planktonic microalgae. Diatoms predominated the benthic microalgae with 96.0% of total species occurred. Dominant species were Amphora sp., Cocconeis sp., Coscinodiscus asteromphalus, Coscinodiscus sp., Nitzschia sigma var. intermedia, Nitzschia distans, Navicula spp., Paralia sulcata, Pleurosigma sp. Skeletonema coastatum, and Surirella sp. Among them, Amphora sp., Paralia sulcata, and species of Pleurosigma and Nitzschia were observed throughout the studied period. Planktonic microalgae of Gamami Beach was also predominated by diatoms. They occupied 88.1% of total planktonic microalgae. The density of microalgal population was higher in silty sediment than in sandy sediment. The population density of microalgae was higher in high tide zone than that in low tide zone. The density of the benthic microalgae in the surface layer of tidal flats showed increasing tendency for 2 hours after the beginning of ebb tide. On the contrary, benthic microalgal density of subsurface layer was decreased during the period. Concentrations of chlorophyll ${\alpha}$ from sediment and water were not synchronized during the study period. Therefore, the distributional patterns of the benthic microalgae and planktonic algae seemed not to be related. Chlorophyll ${\alpha}$ of water was highly related with the concentration of NH$_4$-N, whereas, chlorophyll ${\alpha}$ of sediment uas related with NO$_3$-N concentration.

• 대한기계학회논문집 C: 기술과 교육
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• 제4권2호
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• pp.117-122
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• 2016

세계적으로 무인기 활용이 증가하고 이와 관련한 사고 보도도 잇따르고 있는 가운데 각국 정부 당국, 관련 기관 및 산업체 등에서는 무인기 운영 관련 정책 및 규정 제 개정을 위한 노력에 박차를 가하고 있다. 또한 이와 동시에 국제민간항공기구에서 관련 연구조직(UASSG, Unmanned Aerial System Study Group)을 통해 국제 표준 마련을 위한 공동 연구를 진행해 왔다. 최근에는 국제민간항공기구에서 무인기패널체(RPASP, Remotely Piloted Aircraft System Panel)를 조직하여 무인기의 민간공역 통합 운영을 목표로 관련 부속서, 표준 및 권고 등을 제 개정하기 위한 논의를 수행하고 있다. 본 논문에서는 국제민간항공기구의 무인기 정책 연구 및 개발 동향을 조사하고, 이를 통해 국내 무인기 정책 마련을 위해 필요한 사항을 제안하였다. 이는 국제적인 무인기 정책 및 규정 개발 동향을 파악하여 관련 산업개발 방향 설정 및 국내 규정 마련에 기초자료로 활용할 수 있을 것이다.

• 항공우주정책ㆍ법학회지
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• 제35권3호
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• pp.65-93
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• 2020

정보통신기술 발전과 함께 4차 산업혁명 시대에 돌입하면서 신성장 산업으로 무인항공기 산업이 주목받기 시작했다. 소형 드론에서부터 대형 무인항공기까지 규모와 비행 공역의 범위도 다양해지면서 선진국(미국, 유럽)은 유인항공기와 무인항공기의 통합 운용에 대한 계획을 수립하고 있다. 또한, ICAO에서는 무인항공기의 국제기준 및 권고사항 수립을 하기 위해 관련 부속서 개정 작업을 수행하고 있다. 우리나라도 앞으로 도래할 유인항공기와 무인항공기의 통합 운영에 대비가 필요하여 이를 위해 무인항공기 안전운항에 대한 국내 제도 현황을 검토하고 정비해야 한다. 본 연구는 ICAO에서 논의하고 있는 원격조종항공기시스템(Remotely Piloted Aircraft System; RPAS) 관련 부속서의 개정사항들에 대해 분석하고 기존의 항공안전법과 비교하여 무인항공기 안전운항을 위한 항공안전법의 개정방향을 제시하였다.

• 동의신경정신과학회지
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• 제20권2호
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• pp.121-131
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• 2009

Objectives : From Scrophularia buergeriana water extract(SBW), has been used in vivo test for its beneficial effects on neuronal survival and neuroprotective functions, particularly in connection with APP-related dementias and Alzheimer's disease(AD). $A{\beta}$ oligomer derived from proteolytic processing of the ${\beta}$-amyloid precursor protein(APP), including the amyloid-${\beta}$ peptide($A{\beta}$), play a critical role in the pathogenesis of Alzheimer's dementia. Methods : Using drosophila APP model on APP-induced neuronal cytotoxicity, we demonstrated that SBW prevents neurotoxicity of $A{\beta}$ oligomer, which are the behavior, and possibly causative, feature of AD. We investigated the neuroprotective effects of SBW against the effects of oligomeric $A{\beta}$ and fly behaveior and life span by UAS-GRIM/APP-GAL within transgenic flies. Results and Conclusions : SBW repaired damage leading to the behaveior of APP-induced fly and delayed life span. These results suggest that neuronal damage in AD might be due to two factors: a direct $A{\beta}$ oligomer toxicity and multiple cellular and molecular neuroprotective mechanisms, including attenuation of apoptosis and direct inhibition of $A{\beta}$ oligomer, underlie the neuroprotective effects of SBW.

• 동의생리병리학회지
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• 제22권5호
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• pp.1215-1222
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• 2008

Recent studies indicate that the deposition of ${\beta}$-amyloid ($A{\beta}$) is associated with the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is not clear yet. To investigate the effects of Jangwonhwangagambang (JWHG) extract on AD pathogenicity, we have generated transgenic Drosophila model in which GMR-APP-GAL4/UAS-GRIM system was designed to overexpress amyloid precursor protein(APP), We examined fly's survival ratio, flight behavior, and morphological patterns of chest and eye. We found that JWHG treatment improved fly's survival ratio by inhibiting apoptosis and flight behavior. APP-GRIM transgenic flies treated with JWHG showed had significantly lower levels of APP deposition in the chest and eye compared to control animals. JWHG treatment further inhibited chest and eye degeneration. These results suggest that JWHG prevents APP-induced neurotoxicity, and thus may be applicable for the development of preventive or therapeutic agents for AD treatment.

• 설비공학논문집
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• 제17권3호
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• pp.250-255
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• 2005

The objective of this paper is to develop a new energy transport system for district cooling application by using type 2 absorption cycle. Cold energy from the LNG storage system is utilized as the cooling source of the condenser and the rectifier. The pressures of the system, UAs of the evaporator and the desorber, and the inlet temperatures of the refrigerant to each component are considered as the key parameters. The results show that UA of the evaporator is more dominant parameter on COP than that of the desorber and the optimum system pressure for the demand side is estimated as 525 kPa. For the present system, it is recommended that the refrigerant inlet temperature of the evaporator be lower than $4.3^{\circ}C$ for long-distance transportation. It is concluded that the cold energy from the LNG storage system can be effectively applied to the long-distance transportation system for district cooling application with the type 2 absorption cycle. The optimum operation conditions are also predicted from the parametric analysis.