• Journal of Genetic Medicine
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• v.20 no.2
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• pp.39-45
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• 2023

As advanced sequencing technologies continue to uncover an increasing number of variants in genes associated with human genetic diseases, there is a growing demand for systematic approaches to assess the impact of these variants on human development, health, and disease. While in silico analyses have provided valuable insights, it is essential to complement these findings with model organism studies to determine the functional consequences of genetic variants in vivo. Drosophila melanogaster is an excellent genetic model for such functional studies due to its efficient genetic technologies, high gene conservation with humans, accessibility to mutant fly resources, short life cycles, and cost-effectiveness. The traditional GAL4-UAS system, allowing precise control of gene expression through binary regulation, is frequently employed to assess the effects of monoallelic variants. Recombinase medicated cassette exchange or CRISPR-Cas9-mediated GAL4 insertion within coding introns or substitution of gene body with Kozak-Gal4 result in the loss-of-function of the target gene. This GAL4 insertion strategy also enables the expression of reference complementary DNA (cDNA) or cDNA carrying genetic variants under the control of endogenous regulatory cis elements. Furthermore, the CRISPR-Cas9-directed tissue-specific knockout and cDNA rescue system provides the flexibility to investigate candidate variants in a tissue-specific and/or developmental-timing dependent manner. In this review, we will delve into the diverse genetic techniques available in Drosophila and their applications in diagnosing and studying numerous undiagnosed diseases over the past decade.

• Animal cells and systems
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• v.6 no.3
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• pp.271-275
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• 2002

Rph1 and Gisl are damage-responsive repressors involved in PHR1 expression. They have two $C_{2}$H/ sub 2/ zinc finger motifs as putative DNA binding domains and N-terminal conserved domain with unknown function. They are also found in the human retinoblastoma binding protein 2 and the mouse jumonji- encoded protein. The repressors are able to bind to A $G_{4}$ sequence within a 39-bp sequence called upstream repressing sequence of PHR1 promoter (UR $S_{PHR1}$) responsible for the damage-response of PHR1. We report here that Rph1 is predominantly localized in the nucleus as examined by fluorescence microscopic analysis with GFP-Rph1 fusion protein. On the basis of the fact that the A $G_{4}$ sequence that is recognized by Rph1 and Gisl is also recognized by Msn2 and Msn4 in a process of stress response, we a1so tried to examine the in vivo function of A $G_{4}$ and the role of Msn2 and Msn4 in PHR1 expression. Our results demonstrate that Msn2 and Msn4 are actually required for the basal transcription of PHR1 expression but not for its damage induction. When A $G_{4}$ sequence was inserted into the minimal promoter of the cyc1-LacZ reporter, the increased LacZ expression was observed indicating its involvement in transcriptional activation. The data suggest that the A $G_{4}$ is primarily required for basal transcriptional activation of PHR1 or CYC1 promoter through the possible involvement of Msn2 and Msn4. However, since the A $G_{4}$ is also involved in the repression of PHR1 via Rphl and Gisl, it is proposed that A $G_{4}$ functions as either URS or upstream activating sequence (UAS) depending on the promoter context.t.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.5
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• pp.1645-1653
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• 2014

In general, RGB, NIR, and thermal images are used for obtaining geospatial data. Such multiband images are collected via devices mounted on satellites or manned flights, but do not always meet users' expectations, due to issues associated with temporal resolution, costs, spatial resolution, and effects of clouds. We believe high-resolution, multiband images can be obtained at desired time points and intervals, by developing a payload suitable for a low-altitude, auto-piloted UAV. To achieve this, this study first established a low-cost, high-resolution multiband image collection system through developing a sensor and a payload, and collected geo-referencing data, as well as RGB, NIR and thermal images by using the system. We were able to obtain a 0.181m horizontal deviation and 0.203m vertical deviation, after analyzing the positional accuracy of points based on ortho mosaic images using the collected RGB images. Since this meets the required level of spatial accuracy that allows production of maps at a scale of 1:1,000~5,000 and also remote sensing over small areas, we successfully validated that the payload was highly utilizable.

• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.175-182
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• 2002

Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

• Journal of Environmental Science International
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• v.12 no.7
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• pp.715-724
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• 2003

Distributional patterns of microalgae were studied in the tidal flats of Gamami Beach(Young-Gwang, Korea) from November 1999 to 2000 July. The tidal flats of Gamami Beach was composed mainly of sandy sediment. The concentrations of nutrients were low compared with other tidal flats. In the present study, 68 species of microalgal flora were identified. These were comprised of 25 species of benthic microalgae and 59 species of planktonic microalgae. Diatoms predominated the benthic microalgae with 96.0% of total species occurred. Dominant species were Amphora sp., Cocconeis sp., Coscinodiscus asteromphalus, Coscinodiscus sp., Nitzschia sigma var. intermedia, Nitzschia distans, Navicula spp., Paralia sulcata, Pleurosigma sp. Skeletonema coastatum, and Surirella sp. Among them, Amphora sp., Paralia sulcata, and species of Pleurosigma and Nitzschia were observed throughout the studied period. Planktonic microalgae of Gamami Beach was also predominated by diatoms. They occupied 88.1% of total planktonic microalgae. The density of microalgal population was higher in silty sediment than in sandy sediment. The population density of microalgae was higher in high tide zone than that in low tide zone. The density of the benthic microalgae in the surface layer of tidal flats showed increasing tendency for 2 hours after the beginning of ebb tide. On the contrary, benthic microalgal density of subsurface layer was decreased during the period. Concentrations of chlorophyll ${\alpha}$ from sediment and water were not synchronized during the study period. Therefore, the distributional patterns of the benthic microalgae and planktonic algae seemed not to be related. Chlorophyll ${\alpha}$ of water was highly related with the concentration of NH$_4$-N, whereas, chlorophyll ${\alpha}$ of sediment uas related with NO$_3$-N concentration.

• Transactions of the KSME C: Technology and Education
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• v.4 no.2
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• pp.117-122
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• 2016

Ecumenically, RPAS(Remotely Piloted Aircraft System) operation has been increased and the related accidents also have occurred. Accordingly, national authorities, organizations and industry have been striving for amendment and enactment of policy and regulation related to the RPAS(Remotely Piloted Aircraft System) operation. And ICAO had performed the joint study to make international standards through UASSG(Unmanned Aerial System Study Group). Recently this group has been switched to the panel meeting, RPASP(Remotely Piloted Aircraft System Panel). It has been discussed to make the related annex, SARPs(Standards and Recommended Practices) and etc. in RPASP. In this paper, we investigated the trend of study and development for ICAO RPAS policy and regulation. Based on these results, we suggested considerations to prepare domestic policy and regulations.

• The Korean Journal of Air & Space Law and Policy
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• v.35 no.3
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• pp.65-93
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• 2020

With the development of information and communication technology, the unmanned aerial vehicle industry began to attract attention as a new growth industry as it entered the fourth industrial revolution. As the size of the unmanned aerial vehicles and the scope of airspace vary from small drones to large unmanned aerial vehicles, the developed countries such as USA and Europe are developing plans for the integrated operation of manned and unmanned aerial vehicles. ICAO is also working on amendments to the relevant ICAO annexes to establish international standards and recommendations for unmanned aerial vehicles. Korea also needs to prepare for the integrated operation of manned and unmanned aerial vehicles that will come in the future, and for this purpose, it is necessary to review and revise the national regulation systems for the safe operation of unmanned aerial vehicles. This study analyzes the amendments of related annexes discussed on the Remotely Piloted Aircraft System (RPAS) pannel, and suggests the direction of revision of the Aviation Safety Act for the safe operation of unmanned aerial vehicles in comparison with the existing Aviation Safety Act.

• Journal of Oriental Neuropsychiatry
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• v.20 no.2
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• pp.121-131
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• 2009

Objectives : From Scrophularia buergeriana water extract(SBW), has been used in vivo test for its beneficial effects on neuronal survival and neuroprotective functions, particularly in connection with APP-related dementias and Alzheimer's disease(AD). $A{\beta}$ oligomer derived from proteolytic processing of the ${\beta}$-amyloid precursor protein(APP), including the amyloid-${\beta}$ peptide($A{\beta}$), play a critical role in the pathogenesis of Alzheimer's dementia. Methods : Using drosophila APP model on APP-induced neuronal cytotoxicity, we demonstrated that SBW prevents neurotoxicity of $A{\beta}$ oligomer, which are the behavior, and possibly causative, feature of AD. We investigated the neuroprotective effects of SBW against the effects of oligomeric $A{\beta}$ and fly behaveior and life span by UAS-GRIM/APP-GAL within transgenic flies. Results and Conclusions : SBW repaired damage leading to the behaveior of APP-induced fly and delayed life span. These results suggest that neuronal damage in AD might be due to two factors: a direct $A{\beta}$ oligomer toxicity and multiple cellular and molecular neuroprotective mechanisms, including attenuation of apoptosis and direct inhibition of $A{\beta}$ oligomer, underlie the neuroprotective effects of SBW.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1215-1222
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• 2008

Recent studies indicate that the deposition of ${\beta}$-amyloid ($A{\beta}$) is associated with the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is not clear yet. To investigate the effects of Jangwonhwangagambang (JWHG) extract on AD pathogenicity, we have generated transgenic Drosophila model in which GMR-APP-GAL4/UAS-GRIM system was designed to overexpress amyloid precursor protein(APP), We examined fly's survival ratio, flight behavior, and morphological patterns of chest and eye. We found that JWHG treatment improved fly's survival ratio by inhibiting apoptosis and flight behavior. APP-GRIM transgenic flies treated with JWHG showed had significantly lower levels of APP deposition in the chest and eye compared to control animals. JWHG treatment further inhibited chest and eye degeneration. These results suggest that JWHG prevents APP-induced neurotoxicity, and thus may be applicable for the development of preventive or therapeutic agents for AD treatment.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.17 no.3
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• pp.250-255
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• 2005

The objective of this paper is to develop a new energy transport system for district cooling application by using type 2 absorption cycle. Cold energy from the LNG storage system is utilized as the cooling source of the condenser and the rectifier. The pressures of the system, UAs of the evaporator and the desorber, and the inlet temperatures of the refrigerant to each component are considered as the key parameters. The results show that UA of the evaporator is more dominant parameter on COP than that of the desorber and the optimum system pressure for the demand side is estimated as 525 kPa. For the present system, it is recommended that the refrigerant inlet temperature of the evaporator be lower than $4.3^{\circ}C$ for long-distance transportation. It is concluded that the cold energy from the LNG storage system can be effectively applied to the long-distance transportation system for district cooling application with the type 2 absorption cycle. The optimum operation conditions are also predicted from the parametric analysis.