• Journal of Korean Neurosurgical Society
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• v.46 no.5
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• pp.472-478
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• 2009

Objective : Hyaluronidase (HAse), a degrading enzyme of hyaluronic acid (HA), is highly expressed in patients with malignant glioma. The purpose of this study was to verify whether HAse is related to the invasion of glioma cells. We also investigated if glioma cells with higher mobility in 2-dimensioal (2-D) method have also higher mobility at 3-dimensional (3-D) environment. Methods : Malignant glioma cell lines (U87MG, U251MG, U343MG-A, and U373MG) were used, and their HAse expressions were evaluated by HA zymography. The migration ability was evaluated by simple scratch technique. The invasiveness of each cell lines was evaluated by Matrigel invasion assay and HA hydrogel invasion assay. In HA hydrogel invasion assay, colonies larger than $150\;{\mu}m$ were regarded as positive ones and counted. Statistical analysis of migration ability and invasion properties of each cell lines was performed using t-test. Results : In scratch test to examine migration ability of each cell lines, U87MG cells were most motile than others, and U343MG-A least motile. The HAse was expressed in U251MG and U343MG-A cell lines. However, U87MG and U373MG cell lines did not express HAse activity. In Matrigel invasion assay, the cell lines expressing HAse (U251MG and U343MG-A) were more invasive in the presence of HA than HAse deficient cell lines (U87MG and U373MG). In HA hydrogel invasion assay, the HAse-expressing cell lines formed colonies more invasively than HAse-deficient ones. Conclusion : Malignant Glioma cells expressing HAse were more invasive than HAse-deficient ones in 3-dimensional environment. Therefore, it might be suggested that invasion of malignant gliomas is suppressed by inhibition of HAse expression or HA secretion. Additionally, the ability of 2-D migration and 3-D invasion might not be always coincident to each other in malignant glioma cells.

• Proceedings of the Korea Information Processing Society Conference
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• 2010.11a
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• pp.751-753
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• 2010

본 논문에서는 위상차 현미경 영상 내 U87 세포의 정확한 형태학적 분류를 위한 이진 분류기 구축 방법을 제안한다. 본 방법은 Fourier descriptor 기반 세포형상 표현을 SVM 이진분류기 구축에 사용함으로써 분류 대상인 원추형과 원형세포에 대해 영상 내 세포의 위치와 회전, 크기의 변화에 대해 강인한 분류성능을 제공한다. 본 실험을 통해 polynomial 커널에서 학습된 SVM 분류기가 linear, RBF, sigmoid 에 비교하여 가장 정확한 분류 성능을 보임을 확인하였다. 본 연구는 논문상 기준인 두 종류의 세포 형태 분류기를 기반 프레임워크로 삼아 좀더 다양한 세포 형태를 분류할 수 있도록 개선된다면 악성뇌종양의 전이억제치료에 효과적인 전이행동분석에 도움을 줄 수 있을 것으로 기대된다.

• Cement Symposium
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• s.41
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• pp.82-87
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• 2014

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.7B
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• pp.727-736
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• 2002

In this paper, the broadband microstrip antenna with small size, thin-profile, conformability and ease of manufacture is proposed for providing PCS(down link: 1.75GHz∼1.78GHz, up link: 1.84GHz∼1.87GHz) '||'&'||' IMT-2000(down link: 1.92GHz∼1.98GHz, up link: 2.11GHz∼2.17GHz) services simultaneously. By using double U-slots and matching stub, the bandwidth and operating performance of printed antenna was significantly improved, with need for a complex fabrication procedure. We have also studied the various parameters of the U-slot$_2$for the performances of the antenna. Impedance bandwidth of the wideband microstrip antenna with the double U-slots is about 30.45% (VSWR<2)

• 레미콘
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• no.9 s.17
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• pp.83-87
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• 1988

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.7
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• pp.1022-1028
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• 2013

Chaga mushroom (Inonotus obliquus) was considered a functional food with an anti-cancer effect in colon, gastric, and lung cancer. Therefore, this study was conducted in order to elucidate the effect of chaga mushroom extract in brain cancer. Glioblastoma U-87 MG cells were used in investigation of cell survivability, apoptosis, and cell cycle arrest analysis. Treatment with various concentrations of chaga mushroom extract resulted in inhibition of cell proliferation and cell cycle arrest. Although caspase-3 expression was increased over $100{\mu}g/mL$ of chaga mushroom extract treatment, apoptosis factors with Bcl-2, Bax and p53 did not change. In analysis of cell cycle regulatory factors, expression of cyclin D1 and CDK2 decreased in a dose-dependent manner. We have demonstrated the anti-cancer effect of chaga mushroom extract in glioblastoma, which may be mediated by activation of the caspase pathway and induction of cell cycle arrest.

• IMMUNE NETWORK
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• v.16 no.3
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• pp.183-188
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• 2016

Sirtuin family members with lysine deacetylase activity are known to play an important role in anti-aging and longevity. Cellular senescence is one of the hallmarks of aging, and downregulation of sirtuin is reported to induce premature senescence. In this study, we investigated the effects of small-molecule sirtuin inhibitors on cellular senescence. Various small molecules such as tenovin-1 and EX527 were employed for direct sirtuin activity inhibition. U251, SNB-75, and U87MG glioblastoma cells treated with sirtuin inhibitors exhibited phenotypes with nuclear enlargement. Furthermore, treatment of rat primary astrocytes with tenovin-1 also increased the size of the nucleus. The activity of senescence-associated β-galactosidase, a marker of cellular senescence, was induced by tenovin-1 and EX527 treatment in U87MG glioblastoma cells. Consistent with the senescent phenotype, treatment with tenovin-1 increased p53 expression in U87MG cells. This study demonstrated the senescence-inducing effect of sirtuin inhibitors, which are potentially useful tools for senescence research.

• 대한생식의학회:학술대회논문집
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• 2004.11a
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• pp.87-87
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• 2004

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2009.05a
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• pp.87-87
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• 2009

• Proceedings of the Korean Vacuum Society Conference
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• 2001.07a
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• pp.87-87
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• 2001