Browse > Article
http://dx.doi.org/10.3746/jkfn.2013.42.7.1022

Inhibition Effect of Cell Proliferation and Apoptosis by Inonotus obliquus in Human Glioblastoma U-87 MG Cells  

Shin, Jung-A (Division of Clinical Research Medical Institute, Seoul Medical Center)
Park, Joo Hyun (Division of Clinical Research Medical Institute, Seoul Medical Center)
Kim, Sun Hee (Division of Clinical Research Medical Institute, Seoul Medical Center)
Song, Kwan Yong (Division of Clinical Research Medical Institute, Seoul Medical Center)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.7, 2013 , pp. 1022-1028 More about this Journal
Abstract
Chaga mushroom (Inonotus obliquus) was considered a functional food with an anti-cancer effect in colon, gastric, and lung cancer. Therefore, this study was conducted in order to elucidate the effect of chaga mushroom extract in brain cancer. Glioblastoma U-87 MG cells were used in investigation of cell survivability, apoptosis, and cell cycle arrest analysis. Treatment with various concentrations of chaga mushroom extract resulted in inhibition of cell proliferation and cell cycle arrest. Although caspase-3 expression was increased over $100{\mu}g/mL$ of chaga mushroom extract treatment, apoptosis factors with Bcl-2, Bax and p53 did not change. In analysis of cell cycle regulatory factors, expression of cyclin D1 and CDK2 decreased in a dose-dependent manner. We have demonstrated the anti-cancer effect of chaga mushroom extract in glioblastoma, which may be mediated by activation of the caspase pathway and induction of cell cycle arrest.
Keywords
Inonotus obliquus; U-87 MG; proliferation; cell cycle arrest;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Fulda S, Debatin KM. 2006. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene 25:4798-4811.   DOI   ScienceOn
2 Zhang M, Cui SW, Cheung PCK, Wang Q. 2007. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 18: 4-19.   DOI   ScienceOn
3 Farrow SN, Brown R. 1996. New members of the Bcl-2 family and their protein partners. Curr Opin Genet Dev 6:45-49.   DOI   ScienceOn
4 Bae JT, Chang JS, Lee KR. 2002. Effect of Sarcodon aspratus extract on expression of cycle-associated proteins in HepG2 cells. J Korean Soc Food Sci Nutr 31: 329-332.   과학기술학회마을   DOI   ScienceOn
5 Levine AJ, Momand J, Finlay CA. 1991. The p53 tumour suppressor gene. Nature 351: 453-456.   DOI   ScienceOn
6 Finlay CA, Hinds PW, Tan TH, Eliyahu D, Oren M, Levine AJ. 1988. Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life. Mol Cell Biol 8: 531-539.   DOI
7 Hengartner MO. 2000. The biochemistry of apoptosis. Nature 407: 770-776.   DOI   ScienceOn
8 Nicholson DW, Thornberry NA. 1997. Caspases: killer proteases. Trends Biochem Sci 22: 299-306.   DOI   ScienceOn
9 Kim YK, Kim HJ, Kwon CH, Kim JH, Woo JS, Jung JS, Kim JM. 2005. Role of ERK activation in cisplatin-induced apoptosis in OK renal epithelial cells. J Appl Toxicol 25:374-382.   DOI   ScienceOn
10 Ye M, Liu JK, Lu ZX, Zhao Y, Liu SF, Li LL, Tan M, Weng XX, Li W, Cao Y. 2005. Grifolin, a potential antitumor natural product from the mushroom Albatrellus confluens, inhibits tumor cell growth by inducing apoptosis in vitro. FEBS Lett 579: 3437-3443.   DOI   ScienceOn
11 Hiddemann W, Schumann J, Andreef M. 1984. Convention on nomenclature for DNA cytometry. Cytometry 5: 445-446.   DOI
12 Kuo ML, Huang TS, Lin JK. 1996. Curcumin, an antioxidant and anti-tumor promoter, induces apoptosis in human leukemia cells. Biochim Biophys Acta 1317: 95-100.   DOI   ScienceOn
13 Beaulieu JF, Quaroni A. 1991. Clonal analysis of sucraseisomaltase expression in the human colon adenocarcinoma Caco-2 cells. Biochem J 280: 599-608.   DOI
14 Rho SN, Hong JY. 1998. Antitumor effect and the change of chemosensitivity of chitosan in human lung cancer cell line. Korean J Nutr 31: 739-746.   과학기술학회마을
15 Kim DW, Kim TY, Park JT, Kim JM. 2001. Apoptosis induced by cisplatin in human glioblastoma A172 cells. J Korean Neurosurg Soc 30: S5-S12.
16 Madajewicz S, Chowhan N, Tfayli A, Roque C, Meek A, Davis R. 2000. Therapy for patients with high grade astrocytoma using intraarterial chemotherapy and radiation therapy. Cancer 88: 2350-2356.   DOI   ScienceOn
17 Vick NA, Khandekar JD, Bigner DD. 1977. Chemotherapy of brain tumors. Arch Neurol 34: 523-526.   DOI   ScienceOn
18 Najafzadeh M, Reynolds PD, Baumgartner A, Jerwood D, Anderson D. 2007. Chaga mushroom extract inhibits oxidative DNA damage in lymphocytes of patients with inflammatory bowel disease. Biofactors 31: 191-200.   DOI
19 Lee IK, Kim YS, Jang YW, Jung JY, Yun BS. 2007. New antioxidant polyphenols from the medicinal mushroom Inonotus obliquus. Bioorg Med Chem Lett 17: 6678-6681.   DOI   ScienceOn
20 Park YM, Won JH, Kim YH, Choi JW, Park HJ, Lee KT. 2005. In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus. J Ethnopharmacol 101: 120-128.   DOI   ScienceOn
21 Pardee AB. 1989. G1 events and regulation of cell proliferation. Science 246: 603-608.   DOI   ScienceOn
22 Elledge SJ. 1996. Cell cycle checkpoints: preventing an identity crisis. Science 274: 1664-1672.   DOI   ScienceOn
23 Alao JP. 2007. The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic invention. Mol Cancer 6: 24.
24 Jo EH, Kim SH, Ra JC, Kim SR, Cho SD, Jung JW, Yang SR, Park JS, Hwang JW, Aruoma OI, Kim TY, Lee YS, Kang KS. 2005. Chemopreventive properties of the ethanol extract of chinese licorice (Glycyrrhiza uralensis) root: induction of apoptosis and G1 cell cycle arrest in MCF-7 human breast cancer cells. Cancer Lett 230: 239-247.   DOI   ScienceOn
25 Sartorius U, Schmitz I, Krammer PH. 2001. Molecular mechanisms of death-receptor-mediated apoptosis. Chembiochem 2: 20-29.   DOI   ScienceOn
26 Kim EJ, Lee YJ, Shin HK, Yoon Park JH. 2006. A study on the mechanisms by which the aqueous extract of Inonotus obliquus induces apoptosis and inhibits proliferation in HT-29 human colon cancer cells. J Korean Soc Food Sci Nutr 35: 516-523.   과학기술학회마을   DOI   ScienceOn
27 Burczyk J, Gawron A, Slotwinska M, Smietana B, Terminska K. 1996. Antimitotic activity of aqueous extracts of Inonotus obliquus. Boll Chim Farm 135: 306-309.
28 Hwang YJ, Noh GW, Kim SH. 2003. Effect of extracts on proliferation and caspase-3 activity in human castro-intestinal cancer cell lines. Korean J Nutr 36: 18-23.   과학기술학회마을
29 Cohen JJ. 1993. Apoptosis. Immunol Today 14: 126-130.
30 Searle J, Kerr JF, Bishop CJ. 1982. Necrosis and apoptosis: Distinct modes of cell death with fundamentally different significance. Pathol Annu 17: 229-259.
31 Okragly A, O'Brien M. 1999. Death $check^{TM}$ assay systems for monitoring apoptosis. Promega Notes 72: 3-6.
32 Burnette WN. 1981. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112: 195-203.   DOI   ScienceOn
33 Youn MJ, Kim JK, Park SY, Kim Y, Park C, Kim ES, Park KI, So HS, Park R. 2009. Potential anticancer properties of the water extract of Inonotus obliquus by induction of apoptosis in melanoma B16-F10 cells. J Ethnopharmacol 121: 221-228.   DOI   ScienceOn
34 Kim HG, Yoon DH, Kim CH, Shrestha B, Chang WC, Lim SY, Lee WH, Han SG, Lee JO, Lim MH, Kim GY, Choi S, Song WO, Sung JM, Hwang KC, Kim TW. 2007. Ethanol extract of Inonotus obliquus inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophage cells. J Med Food 10: 80-89.   DOI   ScienceOn
35 Sung B, Pandey MK, Nakajima Y, Nishida H, Konishi T, Chaturvedi MM, Aggarwal BB. 2008. Identification of a novel blocker of $I{\kappa}B{\alpha}$ kinase activation that enhances apoptosis and inhibits proliferation and invasion by suppressing nuclear factor-${\kappa}B$. Mol Cancer Ther 7: 191-201.