• The KIPS Transactions:PartA
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• v.16A no.5
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• pp.381-388
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• 2009

The spread of wireless Internet technology development and applications with location information in the form of location-based services are becoming more diverse. In particular, where you recognize the location of objects such as people and things and to provide valuable services based on the ubiquitous and location-based services are emerging as an important service. The collision between the vehicle position measurement in this thesis and offers related service system. Used in the proposed system, the GPS PACKET with information about the location and time of collision for the vehicle crash, the vehicle consists of a NodeID. Cause a conflict between these data at the vehicle, the vehicle through the gateway from the server to decide whether to go on to determine that an emergency situation, Emergency Center, the location information and giving information about whether the conflict is measured. Also, for such an emergency, such as a family on the outside of the wireless terminal related to Wireless (PDA, Phone) is to let me know. The server to want to save the crash information to the database of configuration. Additionally, the proposed U-LBS system to verify the validity of the experiment was performed.

• Journal of the Korea Society of Computer and Information
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• v.14 no.12
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• pp.201-208
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• 2009

As the ubiquitous environment is created, the latest ports introduce U-Port services in managing ports generally and embody container's location identification system, port terminal management system, and advanced information exchange system etc. In particular, the location identification system for freight cars and containers provide in real time the information on the location and condition for them, and enables them to cope with an efficient vehicle operation management and its related problems immediately. However, such a system is insufficient in effectively handling with the troubles in a large-scale port including freight car's disorderly driving, parking, stop, theft, damage, accident, trespassing and controlling. In order to solve these problems, this study structures the vehicle positioning system and the image verification system unsing high resolution image compression and AVE/H.264 store and transmission technology, able to mark and identify the vehicle location on the digital map while a freight car has stayed in a port since the entry of an automatic gate, or able to identify the place of accident through image remotely.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1708-1716
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• 2016

Glucose dehydrogenase (GDH) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. In this study, the glucose 1-dehydrogenase B gene (gdhB) was cloned from Bacillus thuringiensis subsp. kurstaki, and wild-type (GDH-BT^WT) and His-tagged (GDH-BT^N-His, GDH-BT^C-His) enzymes were produced in Escherichia coli BL21 (DE3). All enzymes were produced in the soluble forms from E. coli. GDH-BT^WT and GDH-BT^N-His showed high specific enzymatic activities of 6.6 U/mg and 5.5 U/mg, respectively, whereas GDH-BT^C-His showed a very low specific enzymatic activity of 0.020 U/mg. These results suggest that the intact C-terminal carboxyl group is important for GDH-BT activity. GDH-BT^WT was stable up to 65℃, whereas GDH-BT^N-His and GDH-BT^C-His were stable up to 45℃. Gel permeation chromatography showed that GDH-BT^WT is a dimer, whereas GDH-BT^N-His and GDH-BT^C-His are monomeric. These results suggest that the intact N- and C-termini are required for GDH-BT to maintain thermostability and to form its dimer structure. The homology model of the GDH-BT^WT single subunit was constructed based on the crystal structure of Bacillus megaterium GDH (PDB ID 3AY6), showing that GDH-BT^WT has a Rossmann fold structure with its N- and C-termini located on the subunit surface, which suggests that His-tagging affected the native dimer structure. GDH-BT^WT and GDH-BT^N-His regenerated NADPH in a yeast reductase-mediated chiral synthesis reaction, suggesting that these enzymes can be used as catalysts in fine-chemical and pharmaceutical industries.

• Korean Journal Plant Pathology
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• v.11 no.3
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• pp.271-277
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• 1995

The RNA nucleotide sequences of the 3/-noncoding regions (3'-NCRs) of two Korean strains of turnip mosaic virus (TuMV), Ca and cqs, have been determined from their cDNA clones that encompassed the 3'-terminal regions of the viral genomic RNAs. The 3'-NCRs of both strains were 209 nucleotides long, terminated with GAC residues and poly (A) tails. The potential polyadenylational signal motif, UAUGU, was located 140 nucleotides upstream from the poly (A) tail in each of the virus. A highly conserved hexanucleotide sequence [A G U G A/U G/C], which was common in the 3'-NCRs of the potyvirus RNAs, was also found at the regions of 119 bases upstream from the 3'-end. Comparison of the 3'-NCRs of the two Korean isolates with those of four strains from Canada, China and Japan showed significantly identical genotypes (94.3∼99.5%). The secondary structure of three loops with long stems was found within the 3'-NCRs by sequence analysis. The substituted bases in the region among the six TuMV strains did not alter their secondary structures. Length of the 3'-NCRs of the know 11 potyviral RNAs and TuMV RNAs was different from one another and their nucleotide sequences showed 55.7% to 24.0% of homology. The 3'-NCR, therefore, is considered to be useful for phylogenetic studies in potyviruses.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1014-1021
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• 2004

Two thermostable xylanases, designated XynA and XynB, were purified to homogeneity from the culture supernatant of Paenibacillus sp. DG-22 by ion-exchange and gel-filtration chromatography. The molecular masses of xylanases A and B were 20 and 30 kDa, respectively, as determined by SDS-PAGE, and their isoelectric points were 9.1 and 8.9, respectively. Both enzymes had similar pH and temperature optima (pH 5.0-6.5 and $70^{\circ}C$), but their stability at various temperatures differed. Xylanase B was comparatively more stable than xylanase A at higher temperatures. Xylanases A and B differed in their $K_m$ and $V_{max}$ values. XynA had a $K_m$ of 2.0 mg/ml and a $V_{max}$ of 2,553 U/mg, whereas XynB had a K_m$ of 1.2 mg/ml and a $V_{max}$, of 754 U/mg. Both enzymes were endo-acting, as revealed by their hydrolysis product profiles on birchwood xylan, but showed different modes of action. Xylotriose was the major product of XynA activity, whereas XynB produced mainly xylobiose. These enzymes utilized small oligosaccharides such as xylotriose and xylotetraose as substrates, but did not hydrolyzed xylobiose. The amino terminal sequences of XynA and XynB were determined. Xylanase A showed high similarity with low molecular mass xylanases of family 11.

• Journal of information and communication convergence engineering
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• v.7 no.2
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• pp.135-140
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• 2009

The wide deployment of wireless access technologies and the integration of various access network interfaces into a single terminal, allows mobile end-users to be always connected to the IP network, and to use those interface simultaneously. In this paper the CTE provides various access network interfaces capabilities, allowing reception of data over multiple service providers with different characteristics. Considerations for multiple network and service provider environments are regarded as essential for the successful deployment of convergence services in Next Generation Network (NGN). Event Driven Service (EDS) is regarded as a typical convergence service converging different functions of multiple service providers. This paper first describes the deployment model of NGN convergence services for multiple service provider environments. It also covers the service scenario of EDS, a convergence service for multiple service provider environments in NGN. Multiple provider environments stimulates the unified identifier management, namely ubiquitous identification (U-ID), to enable users to be provided convergence services without awareness of multiple provides. Then the designed structure and procedures of U-ID based EDS are given. Finally, the implementation results tested on Korea Advanced Research Network (KOREN) are described.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.6
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• pp.693-700
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• 2003

The effect of the configuration of the nozzle and the impinging surface on the characteristics of the hole-tones has been experimentally investigated. It is found that the plate-tone is a special case of hole-tones, where the hole diameter is zero. The jet velocity range for hole-tones is divided into the low velocity region associated with laminar jet and the high velocity region with turbulent jet. The frequency of the tone is that for the shear layer instability at the nozzle exit or that attainable by a cascade of vortex pairing process with increase of the impinging distance. When the distance is longer than one diameter the frequency decreases to the terminal value near the preferred frequency of the column mode instability, in the range 0.23< $St_d$<0.53, where $St_d$ is the Strouhal number defined by $fd/U_J$, f the frequency, d the nozzle diameter, and $U_J$ the exit velocity. While the convection speed of the downstream vortex, in the present study, is almost constant at low-speed laminar jet, it increases with distance at high-speed turbulent jet. As the frequency increases, the convection speed decreases in the low frequency range corresponding to the preferred mode, in agreement with the existing experimental data for a free jet.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.839-846
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• 1999

An extracellular chitinase-producing bacterial strain induced by colloidal chitin was isolated from sea sand and was identified to be a member of the genus Cytophaga. The chitinase was purified successively by 30-60% ammonium sulfate fractionation, and DEAE-Bio gel A column, Octyl-Sepharose CL-4B column, and DEAE-Bio gel A column chromatographies. The enzyme had a molecular mass of 59.75 kDa, and the amino terminal amino acid sequence was ATPNAPVISW MPTDXXLQNXS. The enzyme acted better on colloidal chitin as a substrate than on chitosan. For colloidal chitin and chitosan (Degree of Acetylation, 15-25%), $K_{cat}$ values were 0.60U/mg and 0.08U/mg, respectively. HPLC analysis of the enzymatic reaction products showed that the chitinase produced mostly N-acetyl-D-glucosarnine and di-N-acetylchitobiose. The optimum temperature and pH for the enzyme were $50^{\circ}C$ and 4.0, respectively. N-Bromosuccinimide and $Hg^{2+}$ inhibited the chitinase activity as much as 90%, and $Sb^{3+}$, diethylpyrocarbonate, and $Ag^{+}$ inhibited it by 50-70%.

• Development and Reproduction
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• v.24 no.2
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• pp.101-111
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• 2020

Coiled-coil domain containing 110 (CCDC110, KM-HN-1) is a protein containing C-terminal coiled-coil domain (CCD) which was previously discovered as a member of the human cancer/testis antigen (CTA). In addition, CCDC110 has both nuclear localization signal sequence and the leucine zipper motif. Although the functional role of CCDC110 has yet to be fully identified, the mRNA expression levels of CCDC110 are known to be highly elevated in various cancer types including testis, implying its relevance to cancer pathogenesis. In this study, we first developed several monoclonal antibody (mAb) hybridoma clones targeting CCDC110 and further isolated clone by characterizing for its specificity using immunoblotting and immunoprecipitation approaches with basal parenchymal sperm cells in testis tissue. Next, using these mAbs, we showed that the Tet-inducible overexpression of CCDC110 protein delayed the entry of G2/M phase in U2-OS osteosarcoma cells. Based on these results, we propose that CCDC110 plays a crucial role in cell cycle progression.

• Animal cells and systems
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• v.6 no.2
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• pp.167-170
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• 2002

The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99％) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.