Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Ectopic Overexpression of Coiled-Coil Domain Containing 110 Delays G2/M Entry in U2-OS Cells

  • Lee, Sue Nyoung (Dept. of Nanobiomedical Science & BK21 PLUS Research Center for Regenerative Medicine, Dankook University) ;
  • Hong, Kyeong-Man (Research Institute, National Cancer Center) ;
  • Seong, Yeon Sun (Dept. of Nanobiomedical Science & BK21 PLUS Research Center for Regenerative Medicine, Dankook University) ;
  • Kwak, Sahng-June (Dept. of Biochemistry, College of Medicine, Dankook University)
  • Received : 2020.04.27
  • Accepted : 2020.05.19
  • Published : 2020.06.30
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Abstract

Coiled-coil domain containing 110 (CCDC110, KM-HN-1) is a protein containing C-terminal coiled-coil domain (CCD) which was previously discovered as a member of the human cancer/testis antigen (CTA). In addition, CCDC110 has both nuclear localization signal sequence and the leucine zipper motif. Although the functional role of CCDC110 has yet to be fully identified, the mRNA expression levels of CCDC110 are known to be highly elevated in various cancer types including testis, implying its relevance to cancer pathogenesis. In this study, we first developed several monoclonal antibody (mAb) hybridoma clones targeting CCDC110 and further isolated clone by characterizing for its specificity using immunoblotting and immunoprecipitation approaches with basal parenchymal sperm cells in testis tissue. Next, using these mAbs, we showed that the Tet-inducible overexpression of CCDC110 protein delayed the entry of G2/M phase in U2-OS osteosarcoma cells. Based on these results, we propose that CCDC110 plays a crucial role in cell cycle progression.

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References

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