• Clinical and Experimental Pediatrics
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• v.45 no.4
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• pp.439-448
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• 2002

Purpose : The aim of this study is to determine and compare the effects of adjunctive therapy with different doses of recombinant human granulocyte-colony stimulating factor(rhG-CSF) on reversing sepsis-associated neonatal neutropenia, and their survival rate in a group I/II-type trial. Methods : RhG-CSF was injected subcutaneously to 10 septic-neutropenic neonates with doses of $10{\mu}g/kg$ from Oct. 1995 to Sep. 1996, and was administered to another 12 septic-neutropenic neonates with doses of $5{\mu}g/kg$ from Oct. 1996 to Sep. 1997. Neutrophilic responses and the outcomes of both groups were compared. Results : In the rhG-CSF $10{\mu}g/kg$ treated group and in the $5{\mu}g/kg$ treated group, the absolute neutrophil count(ANC) was $1,065{\pm}89$($mean{\pm}SEM$) and $1,053{\pm}131$, respectively. The only difference between the two groups was the peak ANC at 48 hours. Eight patients from the remaining nine of rhG-CSF $10{\mu}g/kg$ treated group(88.9％) and ten in $5{\mu}g/kg$ treated group(83.3％) survived the sepsis and were discharged without any problems. Conclusions : RhG-CSF can increase the neutrophil count in critically ill septic neutropenic neonats. The survival rate of both groups were up to 90％. This finding suggests that both doses of rhG-CSF may be effective in a therapeutically useful time frame to treat septic neonates with neonatal neutropenia attributable to bone marrow supression or neutrophil consumption.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.330-342
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• 1998

Benthic environmental parameters were analysed at 40 stations during the period from April 1995 to February 1996. such as water temperature, salinity, and dissolved oxygen (DO)-concentration in the surface and bottom water layers, grain size, chemical oxygen demand (COD), ignition loss, particulate organic carbon (POC) in the sediment of Youngsan River estuary. The water temperature ranged from 4.1 to $29.8^{\circ}C$ in the surface and 4.0 to $20.7^{\circ}C$ in the bottom layers. Salinity ranged from 15.1 to $33.6\%_{\circ}$ in the surface and 31.5 to $33.2\%_{\circ}$ in the bottom layer. The salinity in the outer pan of the study area was higher than that of inner area from autumn to spring, whereas they remained lower in summer. Dissolved oxygen concentration ranged from 5,1 to 11.2 $mg/\ell$ in the surface, and 0.79 to 10,2 $mg/{\ell}$ in the bottom layers. Hypoxic condition ($\le2.0mg/\ell$) was developed in the bottom water layer from Youngsan dike to Mokpo Harhour in summer due to the summer stratification. The surface sediment type was silty clay with a mean grain size of $9.12{\pm}0.45\phi$. The range of COD was from 6.15 to $15.49mgO_2/g$ with a mean of $10.59{\pm}12.64mgO_2/g$. The COD in the inner stations was relatively higher than that of outer stations, and decreased toward the outer part of the study area. Ignition loss (IL) ranged from 3.35 to $15.45\%$ with a mean of $5.96{\pm}1.91\%$. Principal component analysis was carried out from the following five environmental parameters: water temperature, dissolved oxygen in the bottom layer and mean grain size, clay content and COD in the sediment. The forty stations in the study area were classified into three stational groups. Group I was located in the inner part of the estuary characterised by relatively low surface salinity and bottom water temperature, fine sedimemt texture, high organic matter and low dissolved oxygen concentration during the summer. Meanwhile, Group III showing relatively high bottom salinity and water temperature was located in the outer part of the estuary characterising coarse sediment and low organic content in sediment. Group II was between Group I and Group III. The division of the areal groups had high correlations to the DO in the bottom layer and clay content in the sediment.

• The Journal of the Korean life insurance medical association
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• v.4 no.1
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• pp.44-76
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• 1987

Present study was undertaken to establish the modified Broca's indices to estimate standard body weight by using a total of 5,496 insured persons who were medically examined at the Honam Medical Room of Dong Bang Life Insurance Company Ltd. from January, 1983 to January, 1986. The results were as follows: 1. The linear regression equations of body weight to $height^3$ to estimate standard body weight were as follows: In male, for $18{\sim}19$ age group $y=7.272{\times}10^{-6}{\times}x^3+23.560$ for $20{\sim}29$ age group $y=8.187{\times}10^{-6}{\times}x^3+22.031$ for $30{\sim}39$ age group $y=8.627{\times}10^{-6}{\times}x^3+23.169$ for $40{\sim}49$ age group $y=9.561{\times}10^{-6}{\times}x^3+20.994$ for $50{\sim}59$ age group $y=8.604{\times}10^{-6}{\times}x^3+23.081$ and for all ages group $y=7.778{\times}10^{-6}{\times}x^3+25.929$ In female, for $18{\sim}19$ age group $y=8.252{\times}10^{-6}{\times}x^3+18.920$ for $20{\sim}29$ age group $y=7.715{\times}10^{-6}{\times}x^3+22.409$ for $30{\sim}39$ age group $y=8.808{\times}10^{-6}{\times}x^3+21.440$ for $40{\sim}49$ age group $y=9.691{\times}10^{-6}{\times}x^3+21.940$ for $50{\sim}59$ age group $y=12.550{\times}10^{-6}{\times}x^3+11.031$ and for all ages group $y=7.300{\times}10^{-6}{\times}x^3+26.601$ In both sexes, for all ages group $y=8.342{\times}10^{-6}{\times}x^3+22.998$ 2. The modified Broca's index is expressed by formula $\{height(cm)-100\}{\times}K(kg)$. K is obtained from the following formula standard weight to average height estimated $\frac{by\;means\;of\;linear\;regression\;equation(kg)}{\{Average\;height(cm)-100\}{\times}K(kg)}$=1 Author's modified Broca's indices are as follows: In male, for $18{\sim}19$ age group $\{height(cm)-100\}{\times}0.85(kg)$ for $20{\sim}29$ age group $\{height(cm)-100\}{\times}0.90(kg)$ for $30{\sim}39$ age group $\{height(cm)-100\}{\times}0.95(kg)$ for $40{\sim}49$ age group $\{height(cm)-100\}{\times}1.00(kg)$ for $50{\sim}59$ age group $\{height(cm)-100\}{\times}0.95(kg)$ and for all ages group $\{height(cm)-100\}{\times}0.95(kg)$ In female, for $18{\sim}19$ age group $\{height(cm)-100\}{\times}0.90(kg)$ for $20{\sim}29$ age group $\{height(cm)-100\}{\times}0.90(kg)$ for $30{\sim}39$ age group $\{height(cm)-100\}{\times}1.00(kg)$ for $40{\sim}49$ age group $\{height(cm)-100\}{\times}1.05(kg)$ for $50{\sim}59$ age group $\{height(cm)-100\}{\times}1.05(kg)$ and for all ages group $\{height(cm)-100\}{\times}1.00(kg)$ In both sexes, for all age group $\{height(cm)-100\}{\times}0.95(kg)$ 3. Several types of modified Broca's index recommended by author are as follows: I. In male, for $18{\sim}29$ age group $\{height(cm)-100\}{\times}0.90(kg)$ and for $30{\sim}59$ age group $\{height(cm)-100\}{\times}0.95(kg)$ In female, for $18{\sim}29$ age group $\{height(cm)-100\}{\times}0.90(kg)$ and for $30{\sim}39$ age group $\{height(cm)-100\}{\times}1.00(kg)$ II. In male, for all ages group $\{height(cm)-100\}{\times}0.95(kg)$ In female, for all ages group $\{height(cm)-100\}{\times}1.00(kg)$ III. In both sexes, for all ages group $\{height(cm)-100\}{\times}0.95(kg)$ Note: The first type of modified Broca's index is the most precise one in estimating standard body weight among several types established by author. 4. Error of estimated standard body weight appearing by applying modified Broca's indices is generally greater in short build persons than in tall build persons and is more dominant especially in female group.

• Tuberculosis and Respiratory Diseases
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• v.43 no.1
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• pp.46-53
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• 1996

Background: Endotoxin or lipopolysaccharide(LPS) can prime phagocytic cells such as polymorphonuclear leukocytes, monocytes or animal peritoneal macrophages to generate increased amounts of secretory products such as oxygen free radicals and tumor necrosis factor, which play an important role in developing adult respiratory distress syndrome in gram negative sepsis. Human alveolar macrophages(HAM) are continuously exposed to various stimuli inhaled into the alveoli, and the response to LPS might be different in HAM. Therefore, we investigated the effect of LPS pre-exposure on HAM adhered to plastic surface and A549 cell(type II human alveolar epithelial cell line) monolayer. Methods: HAM were isolated from bronchoalveolar lavage fluid from normal lung of the patients with localized lung cancer and esophageal cancer. LPS was exposed to HAM for 2hrs before or after adherence to plastic surface of 24-well Linbro plate and A549 cell monolayer. And then HAM was stimulated with PMA(phorbol myristate acetate) or fMLP(N-formyl-methionylleucyl-phenylalanine). The amount of hydrogen peroxide($H_2O_2$) production in the supernatant was measured on the principle of peroxidase-dependent oxidation of phenol red by hydrogen peroxide. Results: LPS pre-exposure could not enhance $H_2O_2$ production in neither HAM adhered to plastic surface nor one to A549 cell monolayer. But LPS even in the absence of PMA or fMLP stimulation directly increased $H_2O_2$ release in HAM if added after the adherence to A549 cell monolayer. Conclusion: Endotoxin does not prime HAM, but may directly activate HAM adhered to alveolar epithelial cells. Further investagation will be necessary.

• Journal of the Korean earth science society
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• v.18 no.6
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• pp.453-463
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• 1997

The contents of high school 'Common science' textbooks was analyzed qualitatively and quantitatively. Seven common science textbooks were selected and its contents, structure, inquiry, activities, appendix and its characteristics were investigated, and analyzed using the Goal Clusters of Project Synthesis and Romey's indices of text evaluation were calculated. The contents of each unit are not much different among textbooks because they are written according to the curriculum ordinance and textbook guidelines of the Ministry of Education. The textbooks was consist of $471{\sim}519$ pages. It was distribute similarly among the chapter of 'materials', 'forces', lives' and 'earth'. The chapter of 'energy' and 'environment' was treat significantly. The contents and structure of common science is a mere physical consolidation. I make an alternative plan that a topic form. Inquiry activities used in the textbooks are 11 type, however most of that is interpretation of data, experiment, survey and discussion. Ninety six percents of the experiment, belong to the 1st level, four percents of that belong to the 2nd level of the Schwab's inquiry level and there are no activities of the 3rd level. Little attention is given to Goal Cluster I, II, IV in the common science textbooks currently employed. Its content should be broadened to include all Goal Clusters of Project Synthesis. Homey's indices representing the degrees of student involvement. are $0.57{\sim}1.14$ for sentence analysis, $0.60{\sim}1.67$ for figure and diagram analysis, $0.67{\sim}1.50$ for analysis of questions at chapter ends, respectively, student activity per page investigated being $0.6{\sim}0.9$. But chapter summaries cease to repeats the conclusions of the chapter also it be rather formally and inattentively written.

• Journal of Gastric Cancer
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• v.2 no.2
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• pp.73-80
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• 2002

In spite the fact that H. pylori infection might be the causative organisms of acute and chronic gastritis, peptic ulcer diseases and the definition as the class I carcinogen by WHO IARC, still debates exist about the relationship between H. pylori and gastric carcinogenesis. Epidemiological and animal studies demonstrated a link between gastric cancer and chronic infection with H, pylori, but the exact mechanism responsible for the development of gastric cancer in H. pylori-infected patients still remain obscure. In order to declare the clear association, definate evidences like that decrement in the incidence of gastric cancer after the eradication of H. pylori in designated area compared to noneradicated region or the blockade of specific mechanism acting on the carcinogenesis by H. pylori infection. The other way is to identify the upregulating oncogenes or downregulating tumor suppressor genes specifically invovled in H. pylori-associated carcinogenesis. For that, we established the animal models using C57BL/6 mice strain. Already gastric carcinogenesis was developed in Mongolian gerbils infected with H. pylori, but there has been no development of gastric cancer in mice model infected with H. pylori after long-term evaluation. Significant changes such as atrophic gastritis were observed in mice model. However, we could observe the development of mucosal carcinoma in the stomach of transgenic mice featuring the loss of TGF-beta sig naling by the expressions of dominant negative forms of type II receptor specifically in the stomach. Moreover, the incidence of gastric adenocarcinoma was significantly increased in group administered with both MNU and H. pylori infection than MNU alone, signifying that H. pylori promoted the gastric carcinogenesis and there might be host susceptibility genes in H. pylori-associated gastric carcinogenesis. Based on the assumption that chronic, uncontrolled inflammation might predispose to carcinogenesis, there have been several evidences showing chronic atrophic gastritis predisposed to gastric carcinogenesis in H. pylori infection. Although definite outcome of chemoprevention was not drawn after the longterm administration of anti-inflammatory drug in H. pylori infection, the actual incidence of atrophic gastritis and molecular evidence of chemoprevention could be obtained. Selective COX-2 inhibitor was effective in decreasing the development of gastric carcinogenesis provoked by H. pylori infection and carcinogen like in chemoprevention of colon carcinogenesis.

• Tuberculosis and Respiratory Diseases
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• v.51 no.2
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• pp.108-121
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• 2001

Background : The $p16^{INK4a}$ (p16) twnor suppressor gene is frequently inactivated in hwnan non-small cell lung cancers (NSCLCs), predominantly through homozygous deletion or in association with aberrant promotor hypermethylation. Death-associated protein kinase (DAPK) gene influences interferon $\gamma$-induced apoptotic cell death and has important role in metastasis of lung cancer in animal model. Hypermethylation of promoter region of DAP kinase gene may suppress the expression of this gene. Methods : This study was performed to investigate the aberrant methylation of p16 or DAP kinase in 35 resected primary NSCLCs by methylation-specific PCR (MSP), and demonstrated frequency, diagnostic value and clinical implication of aberrant methylation of two genes. Results : Thirty-two cases were male patients, and 3 cases were female patients with an average age was 57. $8{\pm}10.5$ years. The histologic types of lung cancer were 22 of squamous cell carcinoma, 12 of adenocarcinoma, 1 of large cell carcinoma. Pathologic stages were 11 cases of stage I (1 IA, 10 IB), 13 cases of stage II (1 IIA, 12 IIB), and 11 cases of stage III (9 IIIA, 2 IIIB). Regarding for the cancer tissue, p16 aberrant methylation was noted in 13 case of 33 cases (39.4%), DAP kinase in 21 cases of 35 cases (60%). Age over 55 year was associated with p16 aberrant methylation significantly (p<0.05). Methylation status of two genes was not different by smoking history, histologic type, size of tumor, lymph node metastasis and disease progression of lung cancer. There was no correlation between p16 and DAP kinase hypermethylation. Conclusion: This investigation demonstrates that aberrant methylation of p16 tumor suppressor gene or DAP kinase showed relatively high frequency (74.3%) in NSCLCs, and that these genes could be a biologic marker for early detection of lung cancer.

• Tuberculosis and Respiratory Diseases
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• v.52 no.4
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• pp.395-404
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• 2002

Background : Surfactant protein A (SP-A) is important for regulating surfactant secretion, synthesis and recycling. However, It's regulation in vivo is unclear. SP-A has important roles in regulating surfactant metabolism as well as determining its physical properties. Glucocorticoid accelerates the morphologic differentiation of epithelial cells into type II cells and increase the rate of phosphatidylcholine synthesis. Methods : The authors investigated the effects of glucocorticoid on the accumulation of mRNA encoding SP-A and SP-A protein content. Adult rats were given various doses of subcutaneous dexamethasone and sacrificed after 24 hours and one week. SP-A mRNA was measured using a filter hybridization method. The lung SP-A protein content was determined using a double sandwich ELISA assay with polyclonal antiserum raised in rabbits against purified rat SP-A. Results : 1) The accumulation of SP-A mRNA in the dexamethasone treated group 24 hours after 0.2 mg/kg dexamethasone treatment was increased 38.8% compared to the control group. 2) The accumulation of SP-A mRNA in the dexamethasone treated group 1 week after 2 mg/kg dexamethasone treatment was 49.7% higher than the control group(P<0.01). 3) The total lung SP-A level was not altered after 24 hours by the 0.2mg/kg treatment. The total lung SP-A content one week after 2mg/kg dexamethasone administration was 373.7% higher than the control group(P<0.005). Conclusion : Dexamethasone treatment results in an increase in the SP-A mRNA and SP-A protein levels, suggesting that the pretranslational events in vivo may in part contribute to this process.

• Tuberculosis and Respiratory Diseases
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• v.66 no.6
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• pp.444-450
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• 2009

Background: Biomarkers for cancer have several potential clinical uses, including the following: early cancer detection, monitoring for recurrence prognostication, and risk stratification. However, no biomarker has been shown to have adequate sensitivity and specificity. Many investigators have tried to validate biomarkers for the early detection and recurrence of lung cancer. To evaluate plasma G-CSF as such a biomarker, protein levels were measured and were found to correlate with the clinicopathological features of primary lung tumors. Methods: Between December 2006 and May 2008, 100 patients with histologically-validated primary lung cancer were enrolled into this study. To serve as controls, 127 healthy volunteers were enrolled into this study. Plasma G-CSF levels were measured in lung cancer patients using the sandwich ELISA system (R & D inc.) prior to treatment. Results: The mean plasma G-CSF levels were 12.2$\pm$0.3 pg/mL and 46.0$\pm$3.8 pg/mL (mean$\pm$SE) in the normal and in the cancer groups, respectively. In addition, plasma G-CSF levels were higher in patients with early lung cancer than in healthy volunteers (p<.001). Plasma G-CSF levels were higher in patients who were under 65 years old or smokers. Within the cancer group, plasma G-CSF levels were higher in patients with non small cell lung cancer than in patients with small cell lung cancer (p<.05). Overall, plasma G-CSF levels were shown to increase dependent upon the type of lung cancer diagnsosed. In the order from highest to lowest, the levels of plasma G-CSF tended to decrease in the following order: large cell carcinoma, squamous cell carcinoma, adenocarcinoma, and bronchioloalveolar carcinoma. Plasma G-CSF levels tended to be higher in patients with advanced TNM stage than in localized TNM stage (I, II

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.3
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• pp.243-250
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• 1981

Soybean, azuki-bean and mungbean were cultivated at different growing conditions, May 10, June 9, July 9 seeding, and May 10 seeding-short day treatment, to investigate the distribution of flowering date and the variation of pod/flower ratio. The growth habit type of the soybean variety used was determinate, but the azuki-bean and mungbean varieties used showed indeterminate growth habit. The pod/flower ratio ranged 38 to 48% for soybean, 23 to 34% for azuki-bean and 46 to 60% for mungbean along with growing conditions. Flower abscission for soybean and mungbean, and pod abscission for azuki-bean affected more on the number of matured pods. Soybean showed once full blooming period, but azuki-bean and mungbean showed two or three times full blooming period except July 9 seeding plot through the whole growing period. Flowers bloomed just after every full blooming period showed lower value of pod/flower ratio and higher pod/flower ratio was obtained from flowers bloomed before or far after full blooming at plots seeded May 10 and June 9 in three pulse crops. Most full blooming period of soybean coincided within 5 days near the terminal leaf appearance date, and number of flowers bloomed before terminal leaf expansion and matured pods from these flowers were major in every soybean plant at all growing codition plots. No relations were found between terminal leaf appearance and flowering or pod setting patterns of azuki-bean and mungbean.