• Bulletin of the Korean Chemical Society
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• v.32 no.8
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• pp.2549-2552
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• 2011

In this study, the kinetic properties of wild-type and C117D mutant H. influenzae MurA (Hi MurA), which catalyzes the first reaction in the biosynthetic pathway of the cell wall, were characterized. Purified recombinant Hi MurA was active at pH values ranging from pH 5.5 to pH 10, and its $K_m$ (UNAG), $K_m$ (PEP), and $k_{cat}$ values were measured to be 31 ${\mu}M$, 24 ${\mu}M$, and 210 $min^{-1}$, respectively. Hi MurA activity was effectively inhibited by fosfomycin with an $IC_{50}$ value of 60 ${\mu}M$. Hi MurA contains a cysteine residue (C117) at the loop region near the PEP binding, whereas MurA from fosfomycin resistant Mycobaterium tuberculosis or Chlamydia trachomatis contain an aspartate residue instead of the cysteine at the corresponding site. Aspartate substitution of Cys117 in Hi MurA shifted its optimum pH from 7.8 to 6.0. In addition, the $K_m$ values for UNAG and PEP were increased to 160 ${\mu}M$ and 150 ${\mu}M$, respectively, and the $k_{cat}$ value was significantly reduced to 41 $min^{-1}$. Furthermore, the C117D mutant form of Hi MurA was not inhibited by 1 mM fosfomycin. These results indicate that the Cys117 of Hi MurA is the binding site of fosfomycin and plays an important role in the fast turnover of the catalytic reaction.

• Health Policy and Management
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• v.12 no.4
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• pp.1-17
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• 2002

This study was attempted to identify the liquidity trends and determinants of private hospitals in Korea different. Data used in this study were collected from 98 hospitals with complete general data of present conditions as well as financial statements(balance sheets, income statements). They were chosen from hospitals that passed the standardization audit undertaken by the Korean Hospital Association from 1996 to 2000 for the purpose of accrediting training hospitals. The dependent variables in this study were used current ration and quick ratio as a proxy indicator for liquidity. The independent variables were ownership type, hospital type, location, bed size, period of establishment, short-term liabilities to total assets, long-term liabilities to total assets, borrowings to total assets, fixed asset ration, net profit to total assets, operating margin to gross revenue, growth rate of net worth to total assets, total asset turnover, and business risk(volatility of profit). The major findings of this study were as follows. Trends of liquidity(current ratio, quick ratio) had been continuously decreased. Especially, There were very distinct decreasing trends of personal hospitals and less than 300beds, which weakened liquidity. The factors had significant effect on current ratio were short-term debt to total assets(-), fixed asset ratio(-), business risk(+). High short-term debt to total assets, high fixed asset ratio and high business risk significantly decreased in liquidity. The factors that significantly affected on quick ratio were short-term debt to total assets(-), borrowings to total assets(+), fixed asset ratio(-), business risk(+).

• The Korean Journal of Mycology
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• v.23 no.1 s.72
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• pp.71-79
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• 1995

The mechanism of cadmium adaptation and detoxification in Rhizopus oryzae was investigated. The lag phase was lengthened as the concentration of cadmium increased. Detoxication of cadmium were postulated to be primarily operated by the induction of two cadmium binding proteins and increment of inorganic polyphosphate pools in adaptation phase. After adaptation, inorganic polyphosphate system has been involved in turnover and compartmentalization. The secondary system for cadmium adaptation and detoxification might be derepression of ACPase activity and the synthesis of phosphatidyl serine. It has been considered that the overall changes for cadmium adaptation and detoxfication eventually influence on the morphology, resulting in the dispersed filamentous type which may be the most advantageous form.

• Journal of Korean Clinical Nursing Research
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• v.24 no.3
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• pp.336-346
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• 2018

Purpose: This study was conducted to identify nurses'critical thinking disposition, self-leadership and clinical competency in small to medium sized hospitals less than 300beds. Methods: Data were collected using the questionnaire from 227 nurses in Incheon city and Gyeonggi province from March to April, 2017. The data were analyzed using t-test, ANOVA and $Scheff{\acute{e}}^{\prime}s$ test to identify differences in critical thinking disposition, self-leadership and clinical competency. Pearson correlation coefficients were used to identify the correlation among the study variables, and multiple regression was used to identify factors contributing to clinical competency. Results: There were significant differences in critical thinking disposition according to age, marital status, clinical career, career in currently working department and education about leadership. Significant differences in self-leadership were identified according to marital status, work position, working department, work type, education about leadership, and turnover intention. Clinical competency was significantly different depending on age, education, monthly income, work position, career in currently working department, work type, education about critical thinking disposition and education about leadership. Clinical competency was positively correlated critical thinking disposition and self-leadership. Critical thinking deposition, monthly income and self-leadership explained 30.1% of clinical competency of nurses working in small to medium sized hospitals. Conclusion: The results of this study suggest that we need to improve nurses'critical thinking disposition, self-leadership, and the clinical competency.

• Journal of the Korean Association of Geographic Information Studies
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• v.12 no.2
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• pp.23-33
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• 2009

Satellite image is very usefully practiced to predict and analyze physical expansion and change of city. Physical expansion and change of city is closely related to the use of land, and continuous growth management focused on the use of land is essential for sustainable city growth. In this research, the change of land cover and land-use were analyzed with basic input data from 1985 to 2000 according to artificial satellite. Moreover, the land-use turnover rate was understood and expansion trend of urban sprawl in Busan metropolitan city and land-use characteristics of the expansion area. The results are, first, the area for urban region was expanded continuously but areas for agriculture area, forest area, and water area had different changes due to administrative district reform of Busan by each year. Second, the urbanization area in Busan was increased by 3.8% from $92.5km^2$ in 1985 to $167.5km^2$ in 2000. Third, the result of analysis on land-use turnover rate showed that agriculture area was turned into urbanized area the most, and forest area was followed by. Fourth, the result of analysis on database and overlay of buildings in Busan established in 2001 showed that agriculture area are had type 1 and 2 neighborhood living facilities (45.63%), apartment house in forest area (18.49%), and factory in water area (31.84%) with high ratio.

• Korean Journal of Poultry Science
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• v.14 no.1
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• pp.9-13
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• 1987

Three-week-old, broiler-type, mixed sex chicks were divided into replicate groups of 10 birds each and fed for 5 weeks. The wheat bran was defatted and added at 0, 10 and 20% levels. A fourth group received the 20% wheat bran plus a cellulase enzyme added at the level of 0.008%. After a five-week experimental period without a marker a 24-pen battery on the four diets were supplemented with 1% chromic oxide and fed 100g daily. After a 2-day preliminary period, feces were collected three times daily from each diet group for two days at 2, 4 or 8 hours after feeding. At the end of 4 days, within each diet group, birds were randomly selected for slaughter at 2, 4 or 8 hours after feeding and the entire gastrointestinal tract was removed and ligated to form five compartments. The lengths of each segment were measured after straightening, and the gizzard was emptied and weighed. The summarized data showed that the group fed on the high-energy basal diet had the lowest gizzard weight (P〈0.05). Chromium turnover time (minutes) in the each segment and entire GI tract of chicks was not influenced by the high fiber diet or cellulase.

• Analyses & Alternatives
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• v.8 no.1
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• pp.169-196
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• 2024

In the House of the Parliament of Kazakhstan, which members are re-elected several times, while others are only first-term? Existing studies on the Kazakhstan political elites have mainly discussed the effect of clans on the appointment or replacement of elites. These studies have contributed to explaining the characteristics of Kazakhstan's clientelistic political structure, but the analysis of the relationship between political background and elite appointment or replacement is very poor. The purpose of this study is to analyze what characteristics of members have continuity in the 4th to 8th House of the Parliament of Kazakhstan. As a result, members with activities in Communist Party of the Soviet Union had a higher average seniority than those who did not in the 4th, 6th, and 7th House of the Parliament. And Nur Otan members had a higher average seniority than those who did not in 4th and 5th House of the Parliament. On the other hand, there was no difference in average seniority by local political experience, and the difference by elite type was only partially found in the 6th House of the Parliament. These results reflect the president's strategy for parliamentary control in that the parliament is used as a means of solidifying Kazakhstan's political regime as an authoritarian state. The significance of this study is that for the first time it empirically proved who sustains political survival in the House of the Parliament of Kazakhstan.

• Korean Journal of Community Nutrition
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• v.4 no.3
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• pp.466-478
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• 1999

A significant gap can exist between what customers expect in foodservice and what service providers deliver to customers. Reducing the gap and enhancing service quality plays a key role in increasing customer satisfaction and repurchase intentions. The purpose of this study was to investigate the personal and operational characteristics that affect the customer perceptions of service quality, to analyze the overall satisfaction and repurchase intention of customers, and to study the service quality gap between customer and foodservice provides. 427 customers and 278 foodservice providers in 82 fast food and family restaurants were surveyed. T-test, ANOVA, Correlation Analysis, and Multiple Regression were used for statistical analysis, The results of this study were as follows: 1) Among the personal characteristics of customers, sex affected the preception of 'General Management' and 'Reputation', and the expense per person showed a correlation with service quality. 2) Among the operational characteristics, the type of restaurant, months since opening, and the number of seats had a significant impact on service quality, while the seat turnover rate showed a negative correlation with service quality. 3) Among the human resource characteristics, the proportion of part-time employees had a negative correlation with service quality, and in general, the training program for full-time employees led to a higher degree of customer perception of service quality. 4) Six dimensions of service quality accounted for 38.39% of customer satisfaction in Multiple Regression. 5) The overall satisfaction of customers willing to repurchase was significantly higher than that of the non-repurchase customers. 6) The operational characteristics explained over 35% for the service quality gap among the customers and the service providers in Multiple Regression.

• Journal of Korean Neurosurgical Society
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• v.54 no.6
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• pp.461-466
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• 2013

Objective : Although curcumin has a protective effect on bone remodeling, appropriate therapeutic concentrations of curcumin are not well known as therapeutic drugs for osteoporosis. The purpose of this study was to compare the bone sparing effect of treatment of low-dose and high-dose curcumin after ovariectomy in rats. Methods : Forty female Sprague-Dawley rats underwent either a sham operation (the sham group) or bilateral ovariectomy (OVX). The ovariectomized animals were randomly distributed among three groups; untreated OVX group, low-dose (10 mg/kg) curcumin administered group, and high-dose (50 mg/kg) curcumin group. At 4 and 8 weeks after surgery, serum biochemical markers of bone turnover were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae were determined by micro-computed tomography (CT). In addition, mechanical strength was determined by a three-point bending test. Results : High-dose curcumin group showed significantly lower osteocalcin, alkaline phosphatase, and the telopeptide fragment of type I collagen C-terminus concentration at 4 and 8 weeks compared with the untreated OVX group as well as low-dose curcumin group. In the analyses of micro-CT scans of 4th lumbar vertebrae, the high-dose curcumin treated group showed a significant increase in bone mineral densities (p=0.028) and cortical bone mineral densities (p=0.036) compared with the low-dose curcumin treated group. Only high-dose curcumin treated group had a significant increase of mechanical strength compared with the untreated OVX group (p=0.015). Conclusion : The present study results demonstrat that a high-dose curcumin has therapeutic advantages over a low-dose curcumin of an antiresorptive effect on bone remodeling and improving bone mechanical strength.

• Journal of Korean Neurosurgical Society
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• v.52 no.1
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• pp.1-6
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• 2012

Objective : The purpose of this study was to evaluate the different patterns of bone loss between the lumbar spine and the femur after ovariectomy in rats. Methods : Twenty-four female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy (the ovariectomized group). Four and eight weeks after operation, six rats from each of the two groups were euthanized. Serum biochemical markers of bone turnover including osteocalcin and alkaline phosphatase (ALP), which are sensitive biochemical markers of bone formation, and the telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption, were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae and femur were determined by micro-computed tomography. Results : Ovariectomized rats were found to have higher osteocalcin, ALP and CTX levels than sham controls. Additionally, 8 weeks after ovariectomy in the OVX group, serum levels of osteocalcin, ALP and CTX were significantly higher than those of 4 weeks after ovariectomy. Bone loss after ovariectomy was more extensive in the 4th lumbar spine compared to the femur. Bone loss in the 4th lumbar spine was mainly caused by trabecular thinning, but in the femur, it was mainly caused by trabecular elimination. Conclusion : The present study demonstrates different patterns of bone loss between the 4th lumbar spine and the femur in ovariectomized rats. Therefore, when considering animal models of osteoporosis, it is important that bone sites should be taken into account.