• 한국식물병리학회지
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• 제10권2호
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• pp.136-139
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• 1994

Turnip mosaic virus (TuMV) was isolated from Rorippa islandica showing mild mosaic symptom in growing field of Chinese cabbage and radish. Identification of the virus was based on host range, transmission by aphids, electron micrograph, serological reaction and hybridization detection. The virus systemically infected on Chenopodium quinoa, Nicotiana clevelandii, N. glutinosa, Brassica rapa, B. campestris subsp. pekinensis and Raphanus sativus, whereas showed local infection on C. amaranticolor, Gomphrena globosa and Tetragonia tetragonoides. The virus was transmitted by aphid (Myzus persicae). The virus particle was filamentous with 720$\times$12 nm in length, and reacted positively with an antiserum of TuMV in agar gel double duffusion test. In slot-blot hybridization using the digoxigenin(DIG)-labeled RNA probe, TuMV-RNA could be detected in sap of R. islandica infected with the virus. This is the first report of a natural infection of that virus on R. islandica.

• 한국식물병리학회지
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• 제14권3호
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• pp.223-228
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• 1998

Turnip mosaic virus)TuMV-Ca) was isolated from a Chinese cabbage showing severe mosaic and black necrotic spots symptoms in Korea. The virus was identified as a strain of TuMV by its host range test, particle morphology, serology, double stranded RNA analysis. For detection of the virus, reverse transcription and polymerase chain reaction(RT-PCR) was performed with a set of 18-mer TuMV-specific primers to amplify a 876 bp DNA fragment The virus was rapidly detected from total nucleic acids of virus infected tissues as well as native viral RNA of purified virion particles by RT-PCR. Detection limit of the viral RNA by RT-PCR was 10 fg.

• 한국응용곤충학회지
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• 제17권1호
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• pp.33-35
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• 1978

모자익병징을 나타내는 이병시금치를 채집하여 순무모자익 바이러스를 분류 동정하였다. 분리된 순무모자익바이러스를 지표식물에 접종한 결과 담배(B.Y)와 명아주에는 국부병반이 형성되었고 쑥갓, 시금치, 무우에는 모자익 병징이 나타났다. 이병시금치 잎을 착즙하여 순무모자익바이러스의 항혈청과의 혈청반응 시험 결과 양성 반응이 나타났다. 이병엽을 Dip법으로 시료를 제작하여 전자현미경에서 검경한 결과 대부분이 750nm의 사상 입자가 관찰되었다. 시금치에서 순무모자익바이러스의 발생분포는 수원, 안양, 대구, 진주 등 거의 전국적으로 발생하였다

• 한국식물병리학회지
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• 제14권3호
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• pp.220-222
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• 1998

In 1995, we collected stock (Matthiola incana) plants causing mosaic symptoms on leaves and color breakings on flowers in Daekwallyong, Korea. Two viruses were isolated from the infected plants, and identified as cucumber mosaic virus (CMV) and turnip mosaic virus (TuMV) by experiments of host range, serology and electron microscopy. Each of the virus did not produce the same symptoms on the stock seedlings as naturally infected plants caused. When the viruses were coinoculated to the stock seedlings, however, severe mosaic symptoms were observed on leaves, and then the color breakings were expressed on flowers.

• 한국식물병리학회지
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• 제10권3호
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• pp.235-239
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• 1994

Viral RNA was extracted from purified Chinese cabbage strain of turnip mosaic virus (TuMV-Ca) from infected leaves of turnip. Polyadenylated genomic viral RNA was recovered by oligo (dT) cellulose column chromatography and used as a template for the synthesis of complementary DNA (cDNA). Recombinant plasmids contained cDNA ranged from about 900 bp to 2, 450 bp were synthesized. Among the selected 41 transformants, pTUCA31 and pTUCA35 had over 2 Kbp cDNA insert. Restriction endonuclease patterns of the clones examined were very similar among them. Clones pTUCA23 and pTUCA31 were overlapped with pTUA35. The longest clone pTUCA35, encoding 3'-end, showed that it contained two sites for EcoRI, and one site for BamHI, ClaI, HincII, SacI and XbaI, respectively. The restriction mapping indicated that the clone pTUCA35 contained partial nuclear inclusion body gene, complete coding region of the coat protein and 3' untranslated region of TuMV-Ca genomic RNA.

• The Plant Pathology Journal
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• 제29권1호
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• pp.105-109
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• 2013

Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.

• The Plant Pathology Journal
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• 제37권1호
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• pp.1-23
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• 2021

Resistance to diseases caused by turnip mosaic virus (TuMV) in crop species of the family Brassicaceae has been studied extensively, especially in members of the genus Brassica. The variation in response observed on resistant and susceptible plants inoculated with different isolates of TuMV is due to a combination of the variation in the plant resistome and the variation in the virus genome. Here, we review the breadth of this variation, both at the level of variation in TuMV sequences, with one eye towards the phylogeny and evolution of the virus, and another eye towards the nature of the various responses observed in susceptible vs. different types of resistance responses. The analyses of the viral genomes allowed comparisons of pathotyped viruses on particular indicator hosts to produce clusters of host types, while the inclusion of phylogeny data and geographic location allowed the formation of the host/geographic cluster groups, the derivation of both of which are presented here. Various studies on resistance determination in particular brassica crops sometimes led to further genetic studies, in many cases to include the mapping of genes, and in some cases to the actual identification of the genes. In addition to summarizing the results from such studies done in brassica crops, as well as in radish and Arabidopsis (the latter as a potential source of candidate genes for brassica and radish), we also summarize work done using nonconventional approaches to obtaining resistance to TuMV.

• The Plant Pathology Journal
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• 제17권4호
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• pp.201-204
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• 2001

Ultrastructural observation was conducted for the cells of oriental cabbage, Brassica campestris ssp. pekinensis 'Chungawang', inoculated simultaneously with Turnip mosaic virus (TuMV-ACT2-4vq) and Ribgrass mosaic virus (RMV-Ca1dn2) which were known as major destructive viruses of oriental cabbage in Korea. In cells infected with RMV alone, the virus particles were located as bundle or scattering in cytosols and vacuoles, which were typical ultrastructures of tobamovirus. Vessels of xylem were compacted with RMV particles. The cells infected only with TuMV had the cluster of virus particles scarcely and the typical potyvirus inclusions of scrolls, pinwheels, tubes and laminated aggregates in cytosols. The TuMV particles were jammed lineally between tonoplasts. In double infection, the two unrelated viruses of TuMV-ACT2-4vq and RMV-CA1dn2 were located together in a cell, and typical properties of each virus were also observed. The potyvirus inclusions and the tobamovirus particles were mixed entirely in cytoplasm. The virus particles of RMV wre presented strikingly near and in the center of potyvirus inclusions. In vascular cells, the tobamovirus particles were located abundantly than those in single infection. The potyvirus inclusions were embedded in the cluster of RMV particles in phloem parenchyma cells and the vascular elements were degenerated severely.

• 한국응용곤충학회지
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• 제16권2호
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• pp.93-100
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• 1977

무우에서 많이 발생하고 있는 turnip mosaic 대한 성질과 이의 충매전염에 대하여 몇가지를 조사하여 다음과 같은 결과를 얻었다. 1) 수집된 TuMV의 물리적성질은 기존성적과 일치하였고, 시무대근의 이병율이 가장 높았다. 2) TuMV의 매개곤충은 Myzus persicae, Lipaphis erysimi, Aphis gossypii, Aphis craccivora의 4종이었다. 3) TuMV의 진딧물에 의한 전염에서 가장 적당한 처리시간은 Myzus persicae는 1시간절식, 3분흡즙, 1분접종이었으며, Lipaphis erysimi는 2시간절식, 5분흡즙, 3분접종이었고, Aphis gossypii는 1시간절식 3분흡즙, 3분접종이었다. 4) 진딧물의 Probing 횟수는 2시간 절식시킨 진딧물과 절식시키지 않는 것에 큰 차이가 없었고, 절식시킨 진딧물의 전매체가 4분경과시부터 섭식하기 시작하였다. 5) 복숭아 혹 진딧물을 1-2분 간격으로 인위적으로 옮겨주었을 때, 2시간 절식시킨 진딧물의 Probing 휫수가 절식시키지 않은 것의 Probing횟수보다 훨씬 많았고, 2시간 절식시킨 진딧물이 주로 3분이내의 획득흡즙시간후 4분이내에 Virus를 매개하였다.

• 한국식물병리학회지
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• 제11권3호
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• pp.271-277
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• 1995

The RNA nucleotide sequences of the 3/-noncoding regions (3'-NCRs) of two Korean strains of turnip mosaic virus (TuMV), Ca and cqs, have been determined from their cDNA clones that encompassed the 3'-terminal regions of the viral genomic RNAs. The 3'-NCRs of both strains were 209 nucleotides long, terminated with GAC residues and poly (A) tails. The potential polyadenylational signal motif, UAUGU, was located 140 nucleotides upstream from the poly (A) tail in each of the virus. A highly conserved hexanucleotide sequence [A G U G A/U G/C], which was common in the 3'-NCRs of the potyvirus RNAs, was also found at the regions of 119 bases upstream from the 3'-end. Comparison of the 3'-NCRs of the two Korean isolates with those of four strains from Canada, China and Japan showed significantly identical genotypes (94.3∼99.5%). The secondary structure of three loops with long stems was found within the 3'-NCRs by sequence analysis. The substituted bases in the region among the six TuMV strains did not alter their secondary structures. Length of the 3'-NCRs of the know 11 potyviral RNAs and TuMV RNAs was different from one another and their nucleotide sequences showed 55.7% to 24.0% of homology. The 3'-NCR, therefore, is considered to be useful for phylogenetic studies in potyviruses.