Turfgrass Rhizoctonia blight is a severe disease in golf courses in Korea. Attempts were made in 1989 to 1994 to identify the Rhizoctonia species associated with turfgrass blights and also to examine their epidemiology. A total of 120 Rhizoctonia isolates collected were identified as R. solani AG1, R. solani AG2-2, R. cerealis(AG-D) and R. oryzas from brown patch, large patch, yellow patch and white patch, re-spectively. R. solani AG1 was mostly associated with brown patch of cool-season grasses. and most frequently isolated in June through July and also in September. R. solani AG2-2 was isolated exclusively from zoysiagrasses from April to November, most frequently in June through July and October through November. R. cerealis was isolated frequently from both creeping hentgrass in March through April and in November, and zoysiagrass in April and July. Thermophilic R. oryzae was isolated only from creeping bentgrass in August, although with very low frequency. R. solani AG2-2 was strongly pathogenic specifically to Korean lawngrasses(Zoysia japonica, Z.matrella, Z. tenuifolia), but non-pathogenic to creeping bentgrass(Agrostis palustris), bermudagrass (Cynodon dactylon), Kentucky bluegrass(Poa pratensis), perennial ryegrass(Lolium prenne), and creeping red fescue(Festuca rubra subsp. ruhra L.). R. cerealis was strongly pathogenic to zoysiagrass and bentgrass only, but was isolate-specific i.e., from non-pathogenic to pathogenic, for other turfgrasses. The mycelial growth was optimum at relatively high temperature ranges of 25~30$^{\circ}C$ for R.solani AG1, AG2-2 and R. oryzae, while the mycelial growth of R. cerealis was initiated at $^{\circ}C$ and almost ceased at or above $^{\circ}C$.
This study is aimed to understand the effects of temperature, light and GA soaking treatment on the seed germination of perennial ryegrass 'Dandy', creeping hentgrass 'Penlinks', tall fescue 'Arid' and Kentucky hluegrass 'Nasaw'. These experiments are carried in vitro, and their results obtained are summarized as the followings ; The optimum temperature for seed germination of perennial ryegrass 'Dandy' was found to he ranged at 20~30$^{\circ}C$, and the optimum temperature for germination of creeping bentgrass 'Penlinks', tall fescue 'Arid' and kentucky bluegrass 'Nasaw' seeds was found to be ranged at 20~25$^{\circ}C$, whereas the germination percentage under the below or above temperature of this range decreased. The germination of perennial ryegrass 'Dandy' and tall fescue 'Arid' seeds showed no effect on light, and while the germination percentage of creeping hentgrass 'Penlinks' increased more highly under the dark condition than under the light condition. The germination percentage decreased according to the higher temperature. The germination of Kentucky bluegrass 'Nasaw' seeds increased more highly under the dark condition than under the light condition at 20~25$^{\circ}C$, whereas the germination percentage under the dark condition at 15$^{\circ}C$ and 30$^{\circ}C$ decreased. In case of GA soaking treatment of seeds, all the cool season turfgrasses tested showed their own promoting effects on seed germination. The early germination of tall fescue 'Arid' was good without the soaking times. Creeping bentgrass 'Penlinks' and perennial ryegrass 'Dandy' had re-spectively the highest germination percentage at 10min and 1hr soaking treatment, and the longer the soaking time, the lesser the germination percentage. Kentucky bluegrass 'Nasaw' obtained the best results at 24has soaking treatment, and the longer the soaking time, the better the ger-mination percentage.
This study was initiated to evaluate green color retention under three different soil systems. Several turfgrasses were evaluated in multi-layer, USGA, and mono-layer systems. Turfgrass entries were comprised of three cultivars of Korean lawngrass (Zoysia japonica Steud.) as warm-season grass (WSG) and three blends and three mixtures of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.) as cool-season grass (CSG). Significant differences were observed in visual turf color and green color retention among soil systems and turfgrasses. Both the multi-layer and USGA systems were highly associated with better color ratings and longer color retention, as compared with the mono-layer system. Seasonal variation of visual turf color greatly occurred from late December to early spring. CSG exhibited longer color retention than did WSG. The latter maintained green color for approximately 6 months, regardless of the soil system. Spring green-up of Korean lawngrass occurred from early to middle May, while it underwent discoloration from late October to early November. Among the CSGs green-up occurred between early March and early April and leaf color was maintained until middle December to early February. Therefore, the CSGs were green for 8.5 to 11 months, depending on turfgrass and soil system. The mean period of green color duration across all soil systems was approximately 10-11, 9-10 and 8.5-9.0 months for PR, KB and TF, respectively. As for the CSG mixtures, the greater the proportion of PR, the longer the green color retention, while the higher the proportion of TF, the shorter the color retention. There was greater variation in green color duration among the CSGs than the WSGs. Across soil systems, color retention differences of 2 to 6 days were observed for the Korean lawngrass, but 7 to 36 days for the CSGs. These results demonstrate that green color retention varied greatly according to soil systems and also among turfgrasses. Selections of turfgrass and soil system should be made using a concept-oriented approach, when establishing garden, park, soccer field, golf course and other sports field. Information obtained in this study can be used to select soil systems and turfgrasses based on the expected degree of leaf color retention.
This study was conducted to find out withering and dying trends of turfgrasses on the base of tree, and to investigated how much area of turfgrasses withering and dying were progressed on angle of inclination at golf course. Turfgrasses withering and dying on base of trees on slope was extended more to the down-solpe direction than to the up-solpe direction. This trend was common in all experiment plot. Turfgrasses on the base of trees were withered and dead by competing with tree root fur nutrients and moisture in soil. But the trend of the withering and dying in some experiment plots occurred by soil erosion following turfgrass withering and dying occurring on upside ridge. This trend was generally appeared in region of tree community at upside ridge. The form of turfgrass withering and dying on the base of tree in some experiment plots was similar to that of the tree crown. This withering and dying from occurred at the $11^{th}$ experiment plot in Ji San Golf Course and the $6^{th}$ experiment plot in East valley Golf Course.
This study was carried out to evaluate the visual turfgrass's color quality, winter color, and spring green-up under three different soil systems and to make a practical use for sports turf design and construction. Several turfgrasses were evaluated in multi-layer, USGA and mono-layer systems. Turfgrass entries in the study comprised of 3 cultivars from Korean lawngrass (Zoysia japonica Steud.) of typical warm-season grass (WSG) and 3 blends and 3 mixtures from Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.) of cool-season grass (CSG). Significant differences were observed in the turfgrass's color quality, winter color, and spring green-up in the study. Seasonal variation of visual turf color greatly occurred according to soil systems and turfgrasses. Multi-layer and USGA systems were highly associated with better visual color ratings, as compared with mono-layer system. Regardless of soil system, visual turf color in all entries was better from spring to fall than in winter. Great color differences were observed during a period of early December to early spring. CSG produced a better color quality over WSG in any soil system. Overall color ratings for CSG were KB > PR > Mixtures > TF. As for a winter color, its ranking was USGA > multi-layer > mono-layer system. No difference was found in winter among cultivars of Korean lawngrass, being completely brown, but great differences among CSG. Rated best for winter color was PR, followed by CSG mixtures, KB and finally TF in order. It was generally conceded that fast green-up in spring was greatly related with multi-layer over mono-layer system and also CSG over WSG. Among CSG, TF had a fastest green-up. PR was also fast in green-up, but poor in color uniformity. KB, however, was the slowest due to shallow rooting system, when compared with other CSGs. These results demonstrate color differences were greatly variable according to soil systems and also among turfgrass species. A precise decision should be made in selecting turfgrass species and soil system. Multi-layer and USGA systems were considered as the suitable one for turfgrass color quality, winter color and spring green-up. It is a great necessity to combine proper soil system, right turfgrass species, and appropriate mixing rates by a concept-oriented approach, when establishing garden, parks, soccer field, and golf courses and so on.
Of 125 isolates collected from 35 golf courses, sensitivity of 44 isolates of Pythium species to metalaxyl was determined on corn meal agar with various concentrations of metalaxyl (0.1, 1.0, 10.0, 50.0, 100.0, and $250.0{\mu}g\;a.\;i/ml$). The isolates were able to be categorized into the sensitive and resistant groups based on hyphal growth measured in terms of colony diameters on the medium with $1.0\;and\;10.0{\mu}g\;a.\;i./ml$. When compared with hyphal growth on the medium without metalaxyl, hyphal growth of the sensitive group which included 31 isolates was inhibited by $66{\sim}98%$ on the medium with $1.0{\mu}g\;a.\;i./ml$, whereas that of the resistant group which included 13 isolates grew well and the hyphal growth was inhibited only by $6{\sim}26%$. When $10.0{\mu}g\;a.\;i./ml$ of metalaxyl was included in the medium, hyphal growth of the sensitive and resistant groups was inhibited by $82{\sim}99%\;and\;27{\sim}47%$, respectively. Occurrence of metalaxyl-resistant isolates of Pythium spp. not only from turfgrasses on golf courses but also from other crops was observed for the first time in Korea. Metalaxyl-resistant isolates occurred most frequently in P. graminicola. Control effects of metalaxyl were determined by applying metalaxyl after and before inoculation of 4 and 3 isolates of sensitive and resistant isolates of P. graminicola, respectively, to creeping bentgrass in pots. The minimum concentration of metalaxyl to control metalaxyl-sensitive isolates was $6.25{\mu}g\;a.\;i./ml$, whereas the disease caused by the metalaxyl-resistant isolates could not be controlled with $12.50{\mu}g\;a.\;i./ml$ of metalaxyl. The disease was controlled more effectively by an application of metalaxyl prior to inoculation than after occurrence of the disease.
Ha, Jong-Won;Hong, Yong;Lee, Sang-Myeong;Choo, Ho-Yul;Kim, Jin-Ho;Lee, Dong-Woon
Asian Journal of Turfgrass Science
/
v.24
no.2
/
pp.191-198
/
2010
Effective sampling method and distribution of earthworms in turfgrasses of golf courses were investigated at 10 Korean golf clubs. Tea tree extract was highly effective in extracting Eisenia andrei compared with mustard oil or formalin. 1000-fold diluent of tea tree extract (4 L/$m^2$) was more effective than 500- and 2000-fold. Sampling efficacy of earthworm increased as soil depth decreased. Additional watering after treatment of tea tree extract did not significantly influence the sampling of earthworms. Eight earthworm species in 4 genera were collected from turfgrasses of 10 golf courses with 4 L of 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$. Among, dominant earthworm was Amynthas heteropodus and collected 147 individuals from 6 golf clubs. Thirteen Drawida japonica was collected from 3 golf clubs and A. hilgendorfi and A. hupeiensis were collected from 2 golf clubs. A. hupeiensis was not collected with 1000-fold diluent of tea tree extract (15% tea saponin)/$m^2$.
Research was initiated to investigate the effect of high water-absorbing polymer on turf grass quality of three major turfgrasses. A total of 12 treatment combinations were used in the study. Treatments were made with different rates of sand, soil organic amendment (SOA), and water-swelling polymer (WSP). Visual turf grass quality was evaluated in creeping bentgrass (Agrostis palustris Huds., CB), Kentucky bluegrass (Poa pratensis L., KB), and zoysiagrass (Zoysia japonica Steud., Zoy) grown under greenhouse conditions. Significant differences were observed among the treatments in CB, KB, and Zoy. Visual quality ratings varied with mixing rates of SOA and WSP, being maximum 5.6 in differences among them. At the end of study it ranged from 0.3 to 9.6 in CB, 0.3 to 4.0 in KB, and 0.9 to 5.8 in Zoy. Turfgrass quality pattern changed with time after seeding among treatments influenced by WSP rates. From this study, a proper rate of WSP is considered to be 5%, 5~10%, and 5% for CB, KB and Zoy, respectively. In general, overall treatment effect of WSP on turfgrass quality was highly associated with SOA 20% in three turtgrass species. When mixing sand with SOA and WSP for rootzone soil, a proper rate of SOA is considered to be 15 to 20% for CB and KB, while 20% for Zoy of warm-season grass. A further study would be required to investigate the effect of varied, gradual mixing rates of WSP on growth characteristics of turfgrasses grown on mixtures of sand, SOA, and WSP before a field application.
Lee, Joon-Hee;Trenholm, Laurie. E.;Unruh, J. Bryan
Asian Journal of Turfgrass Science
/
v.23
no.1
/
pp.9-22
/
2009
Due to increasing concerns over issues with both water quantity and quality for turfgrass use, research was conducted to determine the response of five warm-season turfgrasses to deficit irrigation and to gain a better understanding of relative drought tolerance. St. Augustinegrass(Stenotaphrum secundatum [Walt.] Kuntze.) cultivars 'Floratam' and 'Palmetto', 'SeaIsle 1' seashore Paspalum(Paspalum vaginatumSwartz.), 'Empire' zoysiagrass(Zoysia japonica Steud.), and 'Pensacola' bahiagrass(Paspalum notatum Flugge) were established in lysimeters in the University of Florida Envirotron greenhouse facility in Gainesville. Irrigation was applied at100%, 80%, 60%, or 40% of evapotranspiration(ET). Evaluations included: a) shoot quality, leaf rolling, leaf firing; b) leaf relative water content(RWC), soil moisture content, chlorophyll content index(CCI), canopy photosynthesis(PS); c) multispectral reflectance(MSR); d) root distribution; and e) water use efficiency. Grasses irrigated at 100% and 80% of ET had no differences in visual quality, leaf rolling, leaf firing, RWC, CCI, and PS. Grasses irrigated at 60% of ET had higher values in physiological aspects than grasses irrigated at 40% of ET. 'Sealsle 1' and 'Palmetto' had a deeper root system than 'Empire' and 'Pensacola', while 'Floratam' had the least amount of root mass. Photosynthesis was positively correlated with visual assessments such as turf quality, leaf rolling, leaf firing, and sensor-based measurements such as CCI, soil moisture, and MSR. Reducing the amount of applied water by 20% did not reduce turfgrass quality and maintained acceptable physiological functioning.
The majority of turfgrass composition of golf courses are zoysiagrass, and mingle with cool-season turfgrasses have been increased. As result of mingling the species, invading problem has been raised. In this study, 50 golf courses nationwide were surveyed of grass composition and invading problem with hetero-species of turfgrasses. Among the courses, 47 golf courses have invading problem with more than one species at least. The invading problem has been issued most of golf course in nationwide. Thirty-seven courses composited with zoysiagrass; at 25 courses showed creeping bentgrass invading and at 33 courses were invaded by kentucky bluegrass. Among 47 kentucky bluegrass composition courses, 43 courses had creeping bentgrass invading problem. Invading problem within the grass species (creeping bentgrass in zoysiagrass, kentucky bluegrass in zoysiagrass, creeping bentgrass in kentucky bluegrass); 14, 12, and 21 courses were detected single species, two species, and three species invading problem, respectively.
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