• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• Tunnel and Underground Space
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• v.26 no.1
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• pp.24-31
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• 2016

Recently, many studies have been conducted to use fixed-wing and rotary-wing unmanned aerial vehicles (UAVs, Drones) for topographic surveying in open-pit mines. Because the fixed-wing and rotary-wing UAVs have different characteristics such as flight height, speed, time and performance of mounted cameras, their results of topographic surveying at a same site need to be compared. This study selected a construction site in Yangsan-si, Gyeongsangnam-do, Korea as a study area and compared the topographic surveying results from a fixed-wing UAV (SenseFly eBee) and a popular rotary-wing UAV (DJI Phantom2 Vision+). As results of data processing for aerial photos taken from eBee and Phantom2 Vision+, orthomosaic images and digital surface models with about 4 cm grid spacing could be generated. Comparisons of the X, Y, Z-coordinates of 7 ground control points measured by differential global positioning system and those determined by eBee and Phantom2 Vision+ revealed that the root mean squared errors of X, Y, Z-coordinates were around 10 cm, respectively.

• Tunnel and Underground Space
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• v.24 no.4
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• pp.255-274
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• 2014

The target of this study is large scale rock slope collapsed by around 7 pm on August, 2012, which is located at ${\bigcirc}{\bigcirc}$ limestone quarries of Gangneung city, Gangwondo. The slope prior to the collapse is formed as the height of about 200 m and the average inclination of $45^{\circ}$. The estimated amount of the collapse is about $1,500,000m^3$ with respect to the slope after the collapse. Geotechnical and field investigations such as boring, geophysical prospecting, surface geological survey, geological lineaments, borehole imaging, metric 3D imaging, experimental and field test, mining work by year, and daily rainfall were performed to find the cause of rock slope failure. Various analyzes using slope mass rating, stereonet projection, limit equilibrium method, continuum and non-continuum model were conducted to check of the stability of the slope. It is expected that the cause of slope failure from the results of various analysis and survey is due to the combined factors such as topography, rainfall, rock type and quality, discontinuities, geo-structural characteristics as the limestone cavity and fault zones, but the failure of slope in case of the analysis without the limestone cavity is not occurred. Safe factor of 0.66 was obtained from continuum analysis of the slope considering the limestone cavity, so the ultimate causes of slope failure is considered to be due to the influence of limestone cavity developed along fault zone.

• Tunnel and Underground Space
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• v.23 no.6
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• pp.480-492
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• 2013

After the introduction of geothermal power generation technology based on engineering reservoir creation that can be applied on non-volcanic region, industrial need for studies on the efficient and economic execution of costly deep-depth drilling work becomes manifest increasingly. However, since it is very difficult to predict duration and cost of boring work with acceptable reliability because of many uncertain events during the execution, efficient and organized work management for drilling is not easily achievable. Especially, the round trip that discretely occurs because of the abrasion of bit takes more time as the depth goes deeper and it has a great impact on the work performance. Therefore, a technology that can simulate the occurrence timing and depth of round trip in advance and therefore optimize them is essentially required. This study divided the abrasion state of bit into eight steps for simulation cases and developed a forecast algorithm, i.e., TOSA which can analyze the depth and timing of round trip occurrence. A methodology that can divide a unit section for simulation has been suggested; while the Bourgoyne and Young model has been used for the forecast of drilling rates and bit abrasion extent by section. Lastly, the designed algorithm has been systemized for the convenience of the user.

• Tunnel and Underground Space
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• v.29 no.6
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• pp.468-479
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• 2019

It is essential to comprehend coupled hydro-mechanical behavior to utilize subsurface for the recent demand for underground space usage. In this study, we developed a new simulator for numerical simulation as a tool for researching to consider the various domestic field and subsurface conditions. To develop the new module, we combined OpenGeoSys, one of the scientific software package that handles fluid mechanics (H), thermodynamics (T), and rock and soil mechanics (M) in the subsurface with FLAC3D, one of the commercial software for geotechnical engineering problems reinforced. In this simulator development, we design OpenGeoSys as a master and FLAC3D as a slave via a file-based sequential coupling. We have chosen Terzaghi's consolidation problem related to single-phase fluid flow at a saturated condition as a benchmark model to verify the proposed module. The comparative results between the analytical solution and numerical analysis showed a good agreement.

• Tunnel and Underground Space
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• v.29 no.6
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• pp.439-455
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• 2019

A buffer is the major component of a high level radioactive waste repository. Due to their thermal conductivity and low permeability, bentonites have been considered as a key component of a buffer system in most countries. The deep geological condition generates ground water inflow and results in swelling pressure in the buffer and backfill. Investigation of swelling pressure of bentonite buffer is an important task for the safe disposal system. The swelling pressure that can be critical is affected by mechanical and hydro properties of the system. Therefore, in this study, a sensitivity analysis was conducted to examine the effect of hydro-mechanical (HM) behaviors in the MX-80 bentonite. Based on the results of the swelling pressure generation with HM model parameters, a coupled HM analysis of an unsaturated buffer and backfill in a deep geological repository was also carried out to investigate the major factor of the swelling pressure generation.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.10 no.4
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• pp.295-303
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• 2012

Based on the data observed and analyzed on a groundwater flow system in the KURT (KAERI Underground Research Tunnel) site, the transport of radionuclides, which were assumed to be released at the supposed position, was calculated on the time-domain. A groundwater pathway from the release position to the surface was identified by simulating the groundwater flow model with the hydrogeological characteristics measured from the field tests in the KURT site. The elapsed time when the radionuclides moved through the pathway is evaluated using TDRW (Time Domain Random Walk) method for simulating the transport on the time-domain. Some retention mechanisms, such as radioactive decay, equilibrium sorption, and matrix diffusion, as well as the advection-dispersion were selected as the factors to influence on the elapsed time. From the simulation results, the effects of the sorption and matrix diffusion, determined by the properties of the radionuclides and underground media, on the transport of the radionuclides were analyzed and a decay chain of the radionuclides was also examined. The radionuclide ratio of the mass discharge into the surface environment to the mass released from the supposed repository did not exceed $10^{-3}$, and it decreased when the matrix diffusion were considered. The method used in this study could be used in preparing the data on radionuclide transport for a safety assessment of a geological disposal facility because the method could evaluate the travel time of the radionuclides considering the transport retention mechanism.

• Tunnel and Underground Space
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• v.11 no.3
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• pp.257-269
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• 2001

Numerical simulation is performed to understand the structural behavior of an underground radwaste repository, assumed to be located at the depth of 500 m, in a granitic rock mats, in which a fault intersects the roof of the repository cavern. Two dimensional universal distinct element code, UDEC is used in the analysis. The numerical model includes a granitic rock mass, a canister with PWR spent fuels surrounded by the compacted bentonite inside the deposition hole, and the mixed bentonite backfilled in the rest of the space within the repository cavern. The structural behavior of three different cases, each case with a fault of an angle of $33^{\circ},\;45^{\circ},\;and\;58^{\circ}$ passing through the cavern roof-wall intersection, has been compared. And then fro the case with the $45^{\circ}$ fault, the hydro-mechanical, thermo-mechanical, and thermo-hydro-mechanical interaction behavior have been studied. The effect of the time-dependent decaying heat, from the radioactive materials in PWR spent fuels, on the repository and its surroundings has been studied. The groundwater table is assumed to be located 10m below the ground surface, and a steady state flow algorithm is used.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.39.2-39.2
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• 2011

The new and renewable energy today has a great interest in all countries around the world. In special it has need more limit of the fossil fuel that needs of low carbon emission among the social necessary conditions. Recently, the high-rise building demand the structural safety, the economic feasibility and the functional design. The high-rise building spends enormous energy and it satisfied the design in solving energy requirements. The requirements of energy for the building depends on the partly form wind energy due to the cladding of the building that came from the surroundings of the high-rise building. In this study of the wind energy, the cladding of the building was assessed a tentative study. The wind energy obtains from several small wind powers that came from the building or the surrounding of the building. In making a cladding the wind energy forms with wind pressure by means of energy transformation methods. The assessment for the building cladding was surrounded of wind speed and wind pressure that was carried out as a result of numerical simulation of wind environment and wind pressure which is coefficient around the high-rise building with the computational fluid dynamics. In case of the obtained wind energy from the pressure of the building cladding was estimated by the simulation of CFD of the building. The wind energy at this case was calculated by energy transform methods: the wind pressure coefficients were obtained from the simulated model for wind environment using CFD as follow. The concept for the factor of $E_f$ was suggested in this study. $$C_p=\frac{P_{surface}}{0.5{\rho}V^{2ref}}$$ $$E_c=C_p{\cdot}E_f$$ Where $C_p$ is wind pressure coefficient from CFD, $E_f$ means energy transformation parameter from the principle of the conservation of energy and $E_c$ means energy from the building cladding. The other wind energy that is $E_p$ was assessed by wind power on the building or building surroundings. In this case the small wind power system was carried out for wind energy on the place with the building and it was simulated by computational fluid dynamics. Therefore the total wind energy in the building was calculated as the follows. $$E=E_c+E_p$$ The energy transformation, which is $E_f$ will need more research and estimation for various wind situation of the building. It is necessary for the assessment to make a comparative study about the wind tunnel test or full scale test.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.7
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• pp.552-561
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• 2016

In this experimental study, the dynamic stability derivatives of a tailless lambda-shape UAV are estimated from time history data of aerodynamic moments measured from the internal balance while the test model is forced to oscillate at given frequencies and amplitudes. A 3-axis forced oscillation apparatus is designed to induce decoupled roll, yaw, pitch oscillations respectively. The results show that the roll damping derivatives remain stable at the entire range of angle of attack tested, whereas the pitch damping derivatives become unstable beyond $15^{\circ}$ angle of attack. The amplitude and frequency have little impact on roll damping derivatives while the smaller amplitude and frequency of oscillation improves the pitch stability. The yaw damping derivative values are fairly small as expected for a tailless configuration. The results indicate that the proposed methodology and test apparatus area valid for estimating the dynamic stability derivatives of a tailless UAV.