• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.41 no.6
• /
• pp.427-434
• /
• 2008

Processing of collagen from yellowfin tuna (Thunnus albacares) abdominal skins was optimized by response surface methodology and central composite design. The values of independent variables at optimal conditions were NaOH concentration: 0.5 N, NaOH treatment time: 36.2 hr, pepsin concentration: 1:4.9 ratio (0.245%, w/v), and digestion time: 48.1 hr, respectively. The collagen content estimated under optimal conditions was 33.1%, and the actual experimental collagen content was 32.3%. Physicochemical properties of collagen from yellowfin tuna abdominal skin were investigated by amino acids analysis, SDS-PAGE, FT-IR, viscosity and denaturation temperature. Amino acids content of the collagen was 21.0%. SDS-PAGE pattern of the collagen showed two different $\alpha$-chain (${\alpha}_1$- and ${\alpha}_2$- chain), $\beta$-component and $\gamma$-component. The spectrum of FT-IR of the collagen showed wavenumber at 3,434, 1,650, 1,542 and $1,235\;cm^{-1}$ representing the regions of amide A, I, II and III, respectively. Relative viscosity of the collagen decreased continuously on heating up to $32^{\circ}C$, and the rate of decrease was retarded in the temperature range of $35-50^{\circ}C$. Denaturation temperature (Td) of the collagen solution (0.06%, w/v) was $31^{\circ}C$ and was lower than calf skin collagen ($35^{\circ}C$).

• Korean Journal of Ichthyology
• /
• v.20 no.2
• /
• pp.105-111
• /
• 2008

A pelagic tuna longline research cruise in the eastern and central Pacific Ocean from September to October of 2006 was conducted to compare catch rates with the use of different hook type and bait combinations. Traditional tuna hooks (J 4) and three circle hook types (C15, C16, C18), along with five bait types (chub mackerel (CM), jack mackerel (JM), milkfish (MF), sardine (SD), and squid (SQ)) and hook number as a proxy for hook depth were evaluated for their effect on bigeye tuna catch rates (fish per 1,000 hooks) using Generalized Linear Models (GLMs). Results from 28 sets indicated significant differences in bigeye catch rates between individual longline sets and hook number. The GLM explained 33% of the deviance in bigeye catch rates with these two factors. An alternative model formulation included bait type which had a small effect (explaining 2.7% of the deviance) on catch rates. Hook type had a negligible and non-significant effect in the GLMs. These results indicate that all of the hooks and baits tested are equally effective at catching bigeye tuna and that hook number (depth) was the paramount operational factor in explaining bigeye tuna catch rates.

• Journal of Aquaculture
• /
• v.20 no.1
• /
• pp.19-25
• /
• 2007

For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.

• Fisheries and Aquatic Sciences
• /
• v.19 no.3
• /
• pp.12.1-12.8
• /
• 2016

Roe is the term used to describe fish eggs (oocytes) gathered in skeins and is one of the most valuable food products from fishery sources. Thus, means of processing are required to convert the underutilized yellowfin tuna roes (YTR) into more marketable and acceptable forms as protein concentrate. Roe protein concentrates (RPCs) were prepared by cooking condition (boil-dried concentrate, BDC and steam-dried concentrate, SDC, respectively) and un-cooking condition (freeze-dried concentrate, FDC) from yellowfin tuna roe. The yield of RPCs was in the range from 22.2 to 25.3 g/100 g of roe. RPCs contained protein (72.3-77.3 %), moisture (4.3-5.6 %), lipid (10.6-11.3 %) and ash (4.3-5.7 %) as the major constituents. The prominent amino acids of RPCs were aspartic acid, 8.7-9.2, glutamic acid, 13.1-13.2, and leucine, 8.5-8.6 g/100 g of protein. Major differences were not observed in each of the amino acid. K, S, Na, and P as minerals were the major elements in RPCs. No difference noted in sodium dodecyl sulfate polyacrylamide gel electrophoresis protein band (15-100 K) possibly representing partial hydrolysis of myosin. Therefore, RPCs from YTR could be use potential protein ingredient for human food and animal feeds.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.10 no.1
• /
• pp.37-47
• /
• 1977

Korea started the Atlantic tuna fishery from 1964 by means of longline, then added bait-boats in 1972. Both fisheries have given the top priority to catch yellowfin tuna(Thunnus albacares). The paper reviews available catch, effort and biological data, estimates some population parameters in order to understand the status of the Atlantic yellowfin tuna as a whole. The main findings are summarized as follows: 1. The total of 476 million hook-equivalent fishing effort was thrown to catch yellowfin tuna in 1974, among which one thired was shared by longliners. 2. The dominant age group becomes younger in both surface and longline fisheries. 3. The recent mortality coefficients were calculated as 1.5 for total mortality and 0.7 for fishing mortality. 4. The weight at recruitment was 2.7 kg in 1973 which was smaller than the regulation size(3.2kg) proposed by ICCAT. 5. The maximum sustained yield was calculated to 95-145 thousand metric tons, which was the level of recent catch. Therefore, it is apparent that the present yellowfin tuna fishery should continue to receive close attention.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.47 no.3
• /
• pp.214-221
• /
• 2011

This study was performed to develop a video based system which can be used to measure the averaged fish size in a non-intrusive fashion. The design was based on principles of simple stereo geometry, incorporated fish dimensions weight relationships and took into consideration fish movement to lower system costs. As the fish size is an important factor that impacts the economy of an aquaculture enterprise. Size measurements, including fork length, width or height, girth, thickness and mass, can be used to determine fish condition in the fish farm, so the averaged fish size of fish cage needs to consistently monitor in open ocean aquaculture cage. A precision of ${\pm}3%$ for replicate length measurements of a 60cm bar is obtained at distances between 2.0 and 6.0m, and the mean fork length and mean swimming speed of bluefin tuna were estimated to 48.8cm and 0.78FL/s, respectively.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.16 no.4
• /
• pp.381-386
• /
• 2010

We have cultured the bluefin tuna in Yokjido, Tongyeong with floating cage which was vulnerable to harmful dinoflagellate, Cochiodinium polykrikoides. This study inspected a vertical migration of phytoplankton and Chl-a for reasonable sinking depth in floating cage. Furthermore, we analyzed the fluctuation of the phytoplankton including harmful dinoflagellates occurring in Yokgido for 6 years. Total cell density showed a significant monthly variation and the flora was predominated with diatoms. Gymnodinium sp. occurred 19 times, the greatest number of occurrence in all kinds of dinoflagellates during summer. In particular, the total number of occurrence at C. polykrikoides was 8, and harmful dinoflagellates such as Karenia brevis/Fibrcapsa japonica occurred. The relationship between Chl-a concentration/total cell number and sunset/sunrise was significant and reasonable sinking depth in floating cage was found to be at least 3m from the water surface, which was associated with massive fish kills caused by C. polykrikoides.

• Fisheries and Aquatic Sciences
• /
• v.19 no.3
• /
• pp.14.1-14.10
• /
• 2016

Isoelectric solubilization/precipitation (ISP) processing allows selective, pH-induced water solubility of proteins with concurrent separation of lipids and removal of materials not intended for human consumption such as bone, scales, skin, etc. Recovered proteins retain functional properties and nutritional value. Four roe protein isolates (RPIs) from yellowfin tuna roe were prepared under different solubilization and precipitation condition (pH 11/4.5, pH 11/5.5, pH 12/4.5 and pH 12/5.5). RPIs contained 2.3-5.0 % moisture, 79.1-87.8 % protein, 5.6-7. 4 % lipid and 3.0-3.8 % ash. Protein content of RPI-1 and RPI-2 precipitated at pH 4.5 and 5.5 after alkaline solubilization at pH 11, was higher than those of RPI-3 and RPI-4 after alkaline solubilization at pH 12 (P < 0.05). Lipid content (5.6-7.4 %) of RPIs was lower than that of freeze-dried concentrate (10.6 %). And leucine and lysine of RPIs were the most abundant amino acids (8.8-9.4 and 8.5-8.9 g/100 g protein, respectively). S, Na, P, K as minerals were the major elements in RPIs. SDS-PAGE of RPIs showed bands at 100, 45, 25 and 15 K. Moisture and protein contents of process water as a 2'nd byproduct were 98.9-99.0 and 1.3-1.8 %, respectively. Therefore, yellowfin tuna roe isolate could be a promising source of valuable nutrients for human food and animal feeds.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.29 no.2
• /
• pp.197-207
• /
• 1996

The proportion of log-associated school catches by Korean tuna purse seiners in the western Pacific has shown a declining trend until recent years. During the period $1990\~1995$, log-associated school catches contributed $34.6\%$ to the total Korean tuna purse seine catch, representing quite a low level compared to the early phase of the purse seine fishery. Species compositions of both log-associated and free-school catches showed that skipjack, Katswonus pelamis, was dominant species and yellowfin, Thunnus albacares, followed, with the small amount of bigeye tunas, T. obesus, Yellowfin proportion was higher in free-school catches than in log-associated school catches. Log-associated school catches monitored during the scientific observation period were made of $60\%$ skipjack, $38\%$ yellowfin, and $2\%$ bigeye tunas, indicating the low skipjack and high yellowfin proportion compared with historical fisheries data based on logbooks. A total of 11 by-catch species were identified, of which sharks occurred together with tunas in all sets and yellowtail kingfish was the most abundant by-catch species. From the length distribution it was found that small yellowfin less than 70 cm mainly distributed around floating objects.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.51 no.1
• /
• pp.79-85
• /
• 2015

Three specimens of larvae (5.2~7.8 mm in standard length (SL)), of the family Scombridae, were collected from the southeastern waters off Jeju Island in August, 2014. These specimens were identified to Katsuwonus pelamis have melanophores distributed on the 1st dorsal-fin spines and post ventral margin on caudal peduncle. An analysis of the cytochrome oxidase submit I (COI) sequences (652 base pairs) of mitochondrial DNA showed that our specimens must be K. pelamis, because their sequences were concordant with those of the adult K. pelamis (genetic distance= 0.000~0.002), followed by Auxis rochei (genetic distance= 0.061~0.063) and Euthynnus affinis (genetic distance= 0.077~0.079). During the larval stage, K. pelamis differed from Thunnus spp. species in melanophores distribution period of 1st dorsal-fin spines, lower jaws and ventral margin on caudal peduncle. In conclusion, occurrence of K. pelamis larvae means Korean waters are potential spawning ground of K. pelamis.