• Journal of Microbiology
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• 제44권5호
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• pp.492-501
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• 2006

In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of $40^{\circ}C$. The KNU5377 strain evidenced a very similar growth rate at $40^{\circ}C$ as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at $43^{\circ}C$. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and $H^+$-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures ($43^{\circ}C$), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.

• 한국융합학회논문지
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• 제8권12호
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• pp.445-450
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• 2017

최근 들어 대학에는 중국 유학생들이 증가하고 있으며, 유학생들의 문화적응 스트레스를 감소하여 대학생활 적응을 향상하고자 본 연구에서는 중국유학생들에게 차문화를 통해서 자신을 존중하고 사랑하는 참나를 만나는 참나다례의 직접적 체험과 참나다례 앱에 중국어를 첨가한 참나다례 앱의 융합 프로그램을 적용하였다. P시에 소재한 T대학교 중국 유학생 10명을 대상으로 하여 직접적 참나다례 체험 프로그램 주1회 4회기, 참나다례 앱 보기는 매일 2번 이상 보기로 30일간 진행하였다. 그 결과 프로그램 참여 전 중국 유학생들의 문화적응 스트레스 점수 평균은 98.5(SD 9.63)였고, 프로그램 참여 후 문화적응 스트레스 점수 평균은 93.9(SD 9.67)로 나타나 프로그램 참여 전과 참여 후의 문화적응 스트레스 점수 수준은 유의한 차이가 나는 것으로 확인되었다(Z=6.702***, P=.000). 따라서 본 프로그램은 중국 유학생들에게 문화적응 스트레스를 유의미하게 감소하여 대학생활 적응 향상에 효과적이라고 할 수 있다.

• 여성건강간호학회지
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• 제26권3호
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• pp.205-212
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• 2020

Purpose: This study aimed to identify the components of preterm birth (PTB) through women's personal narratives and to visualize clinical symptom expressions (CSEs). Methods: The participants were 11 women who gave birth before 37 weeks of gestational age. Personal narratives were collected by interactive unstructured storytelling via individual interviews, from August 8 to December 4, 2019 after receiving approval of the Institutional Review Board. The textual data were converted to PDF and analyzed using the MAXQDA program (VERBI Software). Results: The participants' mean age was 34.6 (±2.98) years, and five participants had a spontaneous vaginal birth. The following nine components of PTB were identified: obstetric condition, emotional condition, physical condition, medical condition, hospital environment, life-related stress, pregnancy-related stress, spousal support, and informational support. The top three codes were preterm labor, personal characteristics, and premature rupture of membrane, and the codes found for more than half of the participants were short cervix, fear of PTB, concern about fetal well-being, sleep difficulty, insufficient spousal and informational support, and physical difficulties. The top six CSEs were stress, hydramnios, false labor, concern about fetal wellbeing, true labor pain, and uterine contraction. "Stress" was ranked first in terms of frequency and "uterine contraction" had individual attributes. Conclusion: The text network analysis of narratives from women who gave birth preterm yielded nine PTB components and six CSEs. These nine components should be included for developing a reliable and valid scale for PTB risk and stress. The CSEs can be applied for assessing preterm labor, as well as considered as strategies for students in women's health nursing practicum.

• 한국양식학회지
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• 제14권1호
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• pp.57-64
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• 2001

In Paralichthys olivaceus and Nibea japonica, response to live air (5hr) or ship (25hr) transportation was assessed, by determining the levels of plasma cortisol, glucose, lactic acid and osmolality, as well as hematological parameters namely hematocrit (Ht), red blood cell (RBC) count, hemoglobin (Hb), corpuscular volume (MCV), corpuscular hemoglobin (MCH) and corpuscular hemoglobin concentration (MCHC). In the experimental series I, the olive flounder was subjected to stress or no stress for 1hr, prior to its air transportation for 5hr. The stress suffered by the flounder prior to air transportation resulted in significant reduction in Ht, but increases in MCH and MCHC. Air transportation led to increases in MCV and MCH in both the stressed and non stressed groups. In the non stressed group it led to significant increase in Ht but decrease in MCHC. In the stressed group, the air transportation led to significant increases in osmolality and plasma cortisol from 5 to 38.5ng/$m\ell$. Non stressed groups did not show significant differences in this before and after transportation. In the experiment II, the red blood cell (RBC) count ranged from 2.5$\times$10$^{6}$ /${mu}ell$ to 2.7$\times$10$^{6}$ /${mu}ell$ in the flounder and 1.9$\times$10$^{6}$ /${mu}ell$ to 2.1$\times$10$^{6}$ /${mu}ell$ in the croaker during the pre- and post-transportation, respectively. In the croaker the shipping led to significant increase in plasma cortisol from 26 to 35ng/$m\ell$ but decrease in glucose from 91.0 to 26.4mg/㎗. For glucose the reverse (39.0 to 51.0mg/㎗) was true for the flounder.

• 대한기계학회논문집A
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• 제32권1호
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• pp.21-28
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• 2008

본 연구에서는 인장과 압축실험 데이터를 사용하여 재료의 응력-변형률 곡선을 얻었다. 시편에 네킹 현상이 발생하기 전에는 인장실험의 결과를 토대로 각 재료의 영률값과 항복강도를 찾았다. 이후 비선형 거동은 압축실험의 데이터를 이용해 나타냈다. 이렇게 얻어진 재료의 실제응력-변형률 곡선을 구간 멱함수법을 사용해 변형률 구간별로 회귀하였다. 회귀하여 구한 곡선과 실제 재료의 응력-변형률 곡선을 비교해 최적 회귀방법과 상응하는 변형률 회귀구간을 찾았다. 하나와 두 변수에 의한 회귀를 혼용하면 가장 적절한 회귀 방법이 얻어진다. 우선 두 변수들로 회귀하여 항복강도를 찾는다. 뤼더변형률이 없는 재료의 항복강도를 예측에는 초기구간 데이터만을 이용해야 오차를 최소화 할 수 있다. 한편 뤼더변형률이 재료들은 곡선의 후반부 데이터를 사용해야 정확한 물성치를 찾아낼 수 있다. 이어 항복 강도가 구해진 상황에서 응력-변형률 곡선의 전체 데이터를 사용해, n을 단일변수로 하여 회귀한다. 여기서 얻은 항복강도와 n을 이용하면 실제 실험 응력-변형률 곡선을 가장 유사하게 따라가는 회귀곡선을 얻을 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.116-121
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• 2004

It is known that the transportation stress of market pigs can affect their carcass quality and that blood concentrations of glucose, creatine kinase (CK) and lactate dehydrogenase (LDH) are indicators of the transportation stress. Fifty-seven gilts and 57 barrows weighing approximately 110 kg were randomly assigned into six groups in a 3 [high (0.31 $m^2/100$ kg BW)-, medium (0.35 $m^2$)- and low (0.39 $m^2$)-stocking densities]${\times}2$[1 h vs. 3 h transportation time] arrangement of treatments. Blood samples were taken during transportation and after 2 h lairage. The percentage of "standing" animals during transportation was less in the low- than in the mediumor high-stocking density; the opposite was true for the "sitting" posture. Plasma concentrations of glucose, CK and LDH increased after loading and declined to the resting levels after lairage. Concentrations of CK and LDH were greater in the 3 h vs. 1 h transportation group. Moreover, the LDH concentration was less in the low- than in the medium- or high-density group. Also detected was a significant interaction between the stocking density and transportation time in all of these blood variables. The incidence of pale, soft and exudative (PSE) carcass was greatest in the high-stocking density group. Interestingly, the PSE incidence increased following the 3 h vs. 1 h transportation at the low-density, but not at the medium-density. Results suggest that the medium-density may be preferable to the lowdensity in the long-distance transportation.

• 농업과학연구
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• 제45권2호
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• pp.154-168
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• 2018

Salinity is a major abiotic stress that adversely affects crop productivity and quality. In this study, the metabolic profile and nutritional composition of rice in response to NaCl were analyzed. The plants were exposed to stressed or unstressed conditions, and their metabolic changes were examined in the shoots, roots, and grains collected at different growth stages. The levels of nutrients and anti-nutrients, including proximates, amino acids, fatty acids, minerals, vitamins, and phytic acid, were also determined for the grains. Application of NaCl significantly decreased the shoot and root growth and induced metabolic alterations at the tillering stage. During the heading stage, only the root metabolites were influenced by NaCl, and no metabolic variations related to salinity were found in the shoot, roots, and grains at the ripening stage. Nutritional analysis of the grain samples revealed that the amounts of linolenic acid and tricosanoic acid were significantly reduced while those of copper, sodium, and phytic acid were enhanced in response to stress. However, except for sodium, those differences were not great. Our results suggest that although NaCl-salinity influences the phenotypic and metabolic profiles of rice shoots and roots at the tillering stage, this impact becomes negligible as tissue development proceeds. This is especially true for the grains. Compositional analysis of the grains indicated that salinity induces some changes in fatty acids, minerals, and anti-nutrients.

• 한국습지학회지
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• 제20권3호
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• pp.227-234
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• 2018

본 논문은 점착성 변화에 따른 부유 미립자의 플럭밀도에 대한 물리적 특성을 조사하였다. 플럭밀도에 관한 분석은 바닥전단응력 변화와 이온첨가 조건하에 자유수면이 존재하는 소형 환형수조에서 수행되었다. 미립자 실리카는 퇴적물에 영향을 미치는 점토의 주요 광물 성분이기 때문에 이를 실험재료로써 사용하였다. 연구결과로서 플럭밀도는 바닥전단응력, 염분과 pH값의 증가에 의존함을 나타낸다. 플럭밀도는 정지수면상태에서 염분과 플럭입자가 증가함에 따라 감소하는 반면에 바닥전단응력이 증가하면 정반대로 나타난다. 플럭밀도는 바닥전단응력의 범위가 $0.0086{\sim}0.0132N/m^2$이면 pH4.2에서 보다 pH6.8에서 증가하였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.308-313
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• 2004

SP test has been confirmed the availability, however the application of SP test is hampered because the relation of stress-strain and load-displacement is not determined definitely. This study suggested an evaluation technique of plastic flow characteristic for X20CrMoV121 steel weldment through inverse analysis using SP test and finite element analysis(FEA). From the result, good agreement was found in load-displacement curves obtained from SP test and FEA. Also, The behavior of load-displacement curve from FEA show a rule that load is increase with increasing K(strength coefficient) and displacement is increase with increasing n(work hardening index). From the inverse analysis, true stress-strain curve could be obtained for each local structure of weldment. And the CGHAZ and WM, which showed lower load- displacement behavior, have smaller work hardening index, while FGHAZ have the largest index.

• 한국해양공학회지
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• 제21권2호
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• pp.50-59
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• 2007

Steam generator tubes are degraded from wear, stress corrosion cracking, rupture and fatigue and so on. Therefore, the failure assessment of steam generator tube is very important for the integrity of energy plants. In the steam generator tubes, sometimes, the local wall thinning may result from severe degradations such as erosion-corrosion damage and wear due to vibration. In this paper, the elasto-plastic analysis was performed by FE code ANSYS on steam generator tubes with wall thinning. Also, the four-point bending tests were performed on the wall thinned specimens, and then it was compared with the analysis results. We evaluated the failure mode, fracture strength and fracture behavior from the experiment and FE analysis. Also, it was possible to predict the crack initiation point by estimating true fracture ductility under multi-axial stress conditions at the center of the thinned area from FE analysis.