• Title/Summary/Keyword: Triple Layer

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Cytological Changes Associated with the Exposure of Escherichia coli to Several Disinfectants: An Ultrastructural Study (수종(數種) 항균제(抗菌劑) 처리(處理)에 의(依)한 Escherichia coli의 미세구조(微細構造) 변화(變化))

  • Deung, Young-Kun;Koh, Choon-Myung;Kim, Sung-Kwang
    • The Journal of the Korean Society for Microbiology
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    • v.11 no.1
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    • pp.1-11
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    • 1976
  • An electron microscopic study has been made of the effects of change of cell structure of Escherichia coli treated with several disinfectants. The alterations observed as follows: 1. Nucleoid, cytoplasm which contain ribosomes and cell wall appeared to be composed of a parallel triple. layered membrane can observed in control Escherichia coli. 2. The nuclear material was no longer demonstrable in its normal sites. The cytoplasm lost its granularity, became homogeneous and disruption of cell wall were observed by the treatment with 70% ethyl alcohol and 3% $H_2O_2$. 3. Aggregation of ribosomes and condensation of nuclear material were also observed by the treatment of 5% lysol solution and 1% dodecyl-diamino-ethyl-glycin-hydrochloride. 4. In autoclaved group, the each layer of cell wall was separated and destroyed in some sites where cytoplasm was extracted.

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Sorption of $UO^{2+}_2$ onto Goethite and Kaolinite: Mechanistic Modeling Approach

  • Jinho Jung;Lee, Jae-Kwang;Cho, Young-Hwan;Keum, Dong-Kwon;Hahn, Pil-Soo
    • Nuclear Engineering and Technology
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    • v.31 no.2
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    • pp.182-191
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    • 1999
  • The sorption of UO$_{2}$$^{2+}$ onto goethite and kaolinite under various experimental conditions was successfully interpreted using surface complexation modeling (SCM). The SCM approach used in this work is the triple-layer model (TLM) in which weakly bonded ions are modeled as outer-sphere (ion-pair) complexes and strongly bonded ions as inner-sphere (surface coordination) complexes. The change of ionic strength did not affect the U(VI) sorption onto goethite, thus the formation of inner-sphere surface complexes, (FeO)$_2$UO$_2$ and (FeO)$_2$(UO$_2$)$_3$OH$_{5}$ was assumed to simulate the effects of ionic strength and goethite concentration. On the other hand, the U(VI) sorption onto kaolinite showed ionic strength dependence, thus the formation of AlO-UO$_{2}$$^{2+}$(outer-sphere complex) and SiO(UO$_2$)$_3$OH$_{5}$ (inner-sphere complex) was assumed to simulate the experimental data. In the presence of carbonates, the sorption of U(VI) onto kaolinite decreased in the weakly alkaline pH range. This was well simulated assuming the formation of a outer-sphere surface complex, A1OH$^{2+}$- (UO$_2$)$_2$CO$_3$OH$_3$. Since SCM approach uses thermodynamic data such as surface complexation constants, it is more predictive than empirical modeling approach in which conditional values such as partition coefficient are used. used.

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Hierarchical Control Scheme for Three-Port Multidirectional DC-DC Converters in Bipolar DC Microgrids

  • Ahmadi, Taha;Hamzeh, Mohsen;Rokrok, Esmaeel
    • Journal of Power Electronics
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    • v.18 no.5
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    • pp.1595-1607
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    • 2018
  • In this paper, a hierarchical control strategy is introduced to control a new three-port multidirectional DC-DC converter for integrating an energy storage system (ESS) to a bipolar DC microgrid (BPDCMG). The proposed converter provides a voltage-balancing function for the BPDCMG and adjusts the states of charge (SoC) of the ESS. Previous studies tend to balance the voltage of the BPDCMG buses with active sources or by transferring power from one bus to another. Furthermore, the batteries available in BPDCMGs were charged equally by both buses. However, this power sharing method does not guarantee efficient operation of the whole system. In order to achieve a higher efficiency and lower energy losses, a triple-layer hierarchical control strategy, including a primary droop controller, a secondary voltage restoration controller and a tertiary optimization controller are proposed. Thanks to the multi-functional operation of the proposed converter, its conversion stages are reduced. Furthermore, the efficiency and weight of the system are both improved. Therefore, this converter has a significant capability to be used in portable BPDCMGs such as electric DC ships. The converter modes are analyzed and small-signal models of the converter are extracted. Comprehensive simulation studies are carried out and a BPDCMG laboratory setup is implemented in order to validate the effectiveness of the proposed converter and its hierarchical control strategy. Simulation and experimental results show that using the proposed converter mitigates voltage imbalances. As a result, the system efficiency is improved by using the hierarchical optimal power flow control.

Fabrication and Measurement of Triple U-shaped slot Microstrip Antenna in 5GHz band (5GHz 대역에서 트리플 U-슬롯 모양의 마이크로스트립 안테나 설계 및 제작)

  • Kang, Moon-Kyou;Jung, Yoo-Keun;Ju, Jeong-Min;Jeong, Gyey-Teak;Yoon, Joong-Han;Kwak, Kyung-Sup
    • Journal of IKEEE
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    • v.8 no.1 s.14
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    • pp.86-95
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    • 2004
  • In this paper, we designed, fabricated, and measured antenna results consisting of previous research, that can handle from wireless using-LAN U-shaped slot antenna of HiperLAN bandwidth to ISM band of HiperLAN 1/2 & 5 GHz bandwidth. We presented a new model that structuresof basic U-shaped slot added the same thing twice.After the foam layer is inserted between ground plane and substrate, we got enough bandwidth for VSWR<1.5. The antenna gain was $6.27{\sim}9.82dBi$. The radiation pattern got a stable pattern in frequency bandwidth.

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Effects of $C_2F_{6}$ Gas on Via Etching Characteristics ($C_2F_{6}$ 가스가 Via Etching 특성에 미치는 영향)

  • Ryu, Ji-Hyeong;Park, Jae-Don;Yun, Gi-Wan
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.1
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    • pp.31-38
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    • 2002
  • In order to improve the 0.35 $mutextrm{m}$-via hole etching process the etching characteristic of the gas $C_2F_{6}$ has been analyzed. The samples were triple-layer films(TEOS/SOG/TEOS) on 8-inch wafers and the orthogonal array matrix technique was used for the process. The equipment for etching was the transformer coupled plasma (TCP) source which is a type of high density plasma(HDP). This experiment showed the etching rate for $C_2F_{6}$ was 0.8 $mutextrm{m}$/min-1.1 $mutextrm{m}$/min and the measured uniformity was under $pm$6.9% in the matrix window. The CD skew comparison between pre and post-etching was under 10% which is an outstanding results in the window of profile in anisotropic etching. There was no problem in C2F6 with the flow rate of 20sccm, but when 14sccm of $C_2F_{6}$ was supplied there was a recess problem on the inner wall of SOG film. Consequently the etching characteristic of $C_2F_{6}$ shows a fast etching rate and a very wide process window in HDP TCP.

Isolation of Antibiotic-producing Microorganisms Antagonistic to Soilborne Pathogenic Fungi of Bentgrass and Their Antifungal Activity (잔디 토양전염성병원진균에 대한 길항미생물의 분리 및 길항효과)

  • 이용세;전하준;이창호;송치현
    • Korean Journal of Organic Agriculture
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    • v.6 no.1
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    • pp.133-149
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    • 1997
  • Recently, the importance of management and cultivation of grasses has been increased in Korea. Among these cultural practices, the appropriate control of diseases is considered more important than other cultivation techniques such as fertilization and irrigation. The damages of brown patch and large patch caused by Rhizoctonia spp. and Pythium blight caused by Pythium spp. are serious in the major cultivation area of turfgrass in Korea. Since these diseases are difficult to control by agrochemicals, the damages are very serious if these are occured. The periodic spray of agrochemicals, to protect and control these diseases could make some problems of toxicity and environmental pollution as well as rising of non-target diseases. Therefore, the biological methods to control diseases have been required to decrease problems resulted from overuse of agrochemicals, to conserve natural ecosystem, and to control effectively diseases of grasses in the long period. The number of studies about biological control using antagonistic microorganisms have been increased for last half century. However, the application of biological control method has been very limited. In this study, thirteen isolates of R. cerealis, 8 isolates of R. solani and 3 isolates of Phthyn spp. have been isolated from diseased turfgrass in golf course and grass-culture area that have patch and wilting symptoms of zoysia grass and creeping bentgrass. Isolation frequency of R. cerealis and R. solani was high in especially zoysiagrass, while Pythym spp. was isolated from bent grass at low frequency but showed high pathogenicity. Totally, 205 isolates of soil microorganisms were isolated in this study as primary antagonistic microorganism by Herr's triple agar layer plate and dual culture method using rhizosphere of grasses, soil of crop field as the source of antagonistic microorganisms. Among the 205 isolates, 23 isolates were actinomycetes and 182 isolates were bacteria. All of the actinomycetes were isolated by Herr's method. Antagonistic effect of primary isolated microorganisms was tested for in vitro mycelial growth inhibition against pathogenic fungi isolated from grasses and for inhibition of disease occurrence in 24 well tissue culture plate and pot experiment. Then, four isolated of bacteria which are BG23, BG74, BG136 and BG171 were selected as antagonistic microorganisms against soil-born pathogenic fungi of bentgrass.

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Low Stratospheric Wind Measurement Using Mobile Rayleigh Doppler Wind LIDAR

  • Shu, Zhi-Feng;Dou, Xian-Kang;Xia, Hai-Yun;Sun, Dong-Song;Han, Yan;Cha, Hyunki;Kim, Dukhyeon;Wang, Guo-Cheng;Baik, Sunghoon;Hu, Dong-Dong
    • Journal of the Optical Society of Korea
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    • v.16 no.2
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    • pp.141-144
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    • 2012
  • A mobile Rayleigh Doppler wind LIDAR at an eye-safe wavelength of 355 nm incorporating double-edge technique with triple-channel Fabry-Perot etalon is developed for wind measurement from 5 to 40km. The structure of this LIDAR system is described. An intercomparsion experiment with rawinsonde is made, showing good agreement with expected measurement accuracy. A continuous observation of stratosphere wind field for several days with temporal resolution of 15 min and spatial resolution of 200 m from 5 to 40 km is presented, demonstrating the stability and robustness of the LIDAR. A stratospheric quasi-zero wind layer can be found at around 20 km with a direction change from east to west evident in the continuous observation.

Study on characteristics of thin films for reflection of near infrared light (근적외선 반사 박막 특성 연구)

  • Chung, Youn-Gil;Park, Hyun-Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.6
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    • pp.4121-4124
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    • 2015
  • Near infrared blocking function in energy saving window glass is required. The design, deposition and characteristics of optical thin films for reflection of near-infrared light were studied. The optical thin film is designed as laminated film structure with low refractive index film and high index film. Deposition experiments of $SiO_2$ and $TiO_2$ thin films with designed structure using the RF sputtering method were carried out. The characteristics of the thin film with deposition conditions were analyzed. High-refractive-index thin film of $TiO_2$/low refractive-index thin film of $SiO_2$ and high-refractive-index thin film of $TiO_2$ structure for reflection of near-infrared light was designed to be simulated. Results of simulation showed reflectance of 30% or more in the range from 930nm to 1682nm. Triple layer thin films fabricated with simulated results showed wavelength bands from 930nm to 1525nm for the reflectance of 33% or more.

Development of an Engineering Model of Hydrogen-Fueled Ultra-micro Combustor for UMGT

  • Shimotori, Shoko;Yuasa, Saburo;Sakurai, Takashi
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2008.03a
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    • pp.828-836
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    • 2008
  • To develop an engineering-model of hydrogen-fueled ultra-micro combustor for Ultra Micro Gas Turbine(UMGT), we reviewed and summarized the problems in downsizing combustors, and determined a suitable burning method. The key issue to actualize practical ultra-micro combustors is reducing heat loss from the combustor to compressor and turbine. The reduction of heat loss was discussed from 3 different viewpoints; heat-insulation material, high-space-heating-rate combustion, and combustor-insolated gas turbine structure. Use of heat-insulation material induced the heat loss reduction to the surroundings. The heat loss ratio decreased substantially in reverse proportion to space heating rate, leading the idea that it could be reduced by burning at a high space heating rate. By settling the combustor insolated from the compressor and turbine, the heat transfer from the combustor to the compressor and turbine becomes smaller. For a selection of the suitable burning method, comparison between 2 burning methods, flat-flame and swirling-flamer types, was conducted. Synthetically the flat-flame burning method was confirmed to be more suitable for ultra-micro combustors than latter one. Base on them, an engineering-model of hydrogen-fueled flat-flame ultra-micro combustor was developed. To obtain high overall heat-insulation, heat-resistant and strength, the engineering-model combustor had triple layer structure with an advanced ceramic, a heat insulation material and a stainless steel. To simplify heat transfer issue in the combustor, it was isolated from the other components. Furthermore it was designed by considering structure, size, material, velocity, pressure loss and prevention of flashback.

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Development of Antagonistic Microorganism for Biological Control of Dollar Spot of Turfgrass (잔디 동전마름병의 생물학적 방제를 위한 길항 미생물의 선발과 효력 검정)

  • Shim, Taek-Su;Jung, Woo-Cheol;Do, Ki-Seok;Shim, Gyu-Yul;Lee, Jae-Ho;Choi, Kee-Hyun
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.191-201
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    • 2006
  • Dollar spot caused by Sclerotinia homeocarpa is one of major diseases in putting greens. Microorganisms antagonistic to S. homeocarpa, a pathogen of dollar spot, were primarily screened through in vitro tests, including dual culture method and triple layer agar diffusion method. In vivo tests were also conducted to select the best candidate for a biocontrol microorganism, using pot experiment. Bacillus subtilis EW42-1 and Trichoderma harziaum GBF-0208 were finally selected as biocontrol agents against dollar spot. Relative Performance Index(RPI) was used as a criterion of selecting potential biocontrol agents. B. subtilis EW42-1 and T. harzianum GBF-0208 showed resistance to several agrochemicals mainly used in a golf course. B. subtilis EW42-1 and T. harzianum GBF-0208 suppressed effectively the disease progress of dollar spot like synthetic fungicide tebuconazole in the nursery where dollar spot had seriously occurred. B. subtilis EW42-1 and T. harzianum GBF-0208 have a potential to be biocontrol agents for the control of dollar spot.