• The Plant Pathology Journal
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• v.15 no.6
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• pp.335-339
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• 1999

Citrus tristeza virus(CTV), an aphid-borne closterovirus, is one of the most destructive pathogens of citrus. It has caused rapid decline in growth, stem pitting and death in citrus trees. A reverse transcriptase polymerase chain reaction (RT-PCR) assay was developed for detection of CTV and investigation of the CTV infection status of citrus and its related cultivars in Cheju island. For RT-PCR based CTV detection, primers were designed to amplify 670bp of coat protein gene. A screening test for CTV in citrus cultivars was conducted from March to July in 1999. Seventy individual citrus trees representing 9 species of 3 genera were tested. The infection rates of CTV for leaves from the years or older trees of late maturing citrus varieties such as Yuzu (C. junos Sieb. ex Tanaka), Navel orange (C.sinensis Osbeck), Kiyomitanger (C. unshiu x C. sinensis), and Shiranuhi ((C. unshiu x C. sinensis) x C. reticulata) were 100%, 80%, 60%, and 60% respectively. The CTV infection rates in Early satsuma mandarins such as 'Miyagawa Early' Satsuma mandarins (C. unshiu Marc. var. Miyagawa) and 'Okitsu Early' Satsuma mandarins (C. unshiu Marc. var. Okitsu) were 100%, and 60%, respectively. CTV was not detected in Cheju native Dangyooja (C. unshiu Marc. var. Osbeck), Trifoliate orange (Poncirus trifoliata) and Kumquat (Fortunella margarita Swingle). In conclusion, RT-PCR assay can be successfully applied to the detection of CTV in citrus trees.

• The Plant Pathology Journal
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• v.17 no.3
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• pp.184-188
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• 2001

Phytophthora-induced diseases on citrus in Jeju island have been considered of minor importance because of the use as root stock of trifoliate orange, which is immune to Phytophthora. However, brown rot on fruit, which severely occurred in 1998 and 1999, has become a great threat to citrus production in the island. About one-half of the surveyed orchards were infected in 1998 and 4 out of 19 infected fields showed over 20% fruit infection rate. The disease was less severe in 1999, with an estimated infected area and total fruit reduction of 3,155 ha and 15,300 tons, respectively. Typical gummosis was also occasionally observed on cv. Shiranugi, which is mostly cultivated under plastic film houses. Two types of Phytophthora were consistently isolated from various plant parts, identified as P. citrophthora and P. nicotianae. The former was isolated from the aerial parts of the fruit, young leaf, and shoot in the fields. Meanwhile, the latter was only isolated from the basal stem showing gummosis in plastic film houses.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.188-191
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• 2008

Poncirus trifoliata Rafinesque has been used as the immature fruit of the trifoliate orange tree. This study was carried out to investigate the quality change of Poncirus trifoliata Rafinesque depending on packing materials (vacuum packing, PP (polypropylene) packing, gunny sack packing), storage places and storage periods up to 12 months. The change of loss on drying content, content of poncirin were measured during the 12 months. As a result, the loss on drying content was decreased rapidly in gunny sack packing after storage of 12 months at room temperature. The content of poncirin was decreased generally according to storage conditions and its average loss percent was 38.8%.

• Journal of the Korean Society of Food Culture
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• v.36 no.2
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• pp.168-183
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• 2021

Using the big data analysis of the Choseonwangjosilrok, this research aimed to figure out the fruits' types, prevalence, seasonal appearances as well as the royalty's perspective on fruits during Choseon period. Choseonwangjosilrok included nineteen kinds of fruits and five kinds of nuts, totaling 1,601 cases at 72.8% and 533 cases at 24.2% respectively. The text recorded fruits being used as: tributes for kings, gifts from kings to palace officials, tomb offerings, county specialties, trade goods or gifts to the foreign ambassadors, and medicine ingredients in oriental pharmacy. Seasonally the fruits appeared demonstrating an even distribution. Periodic characteristics were observed in decreasing quantity chronologically. From fifteenth century to nineteenth century, the fruits with timely features were seen: 804 times at 36.6%, 578 times at 26.3%, 490 times at 22.3%, 248 times at 11.3%, and 78 times at 3.5% respectively. In fifteenth century: citrons, quinces, pomegranates, cherries, permissions, watermelons, Korean melons, omija, walnuts, chestnuts, and pine nuts appeared most frequently. In sixteenth century: pears, grapes, apricots, peaches, and hazelnuts appeared most frequently. In seventeenth century: tangerines and dates appeared most frequently. In eighteenth century, trifoliate orange was the most frequently mentioned fruit.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.91-91
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• 2018

Poncirus Fructus (PF) is obtained by drying the trifoliate orange fruit belonging to the Rutaceae family. In our country of medicine, PF has been used as a treatment of indigestion, allergy and inflammation. But Mechanism and medical data for PF is insignificant. Recently, the effect of the study PF of biological activity was reported, such as anti- thrombosis, anti-bacteria, anti-virus, anti- allergic. We investigated that the effect of PF on anti-inflammatory in murine macrophage-like cell line Raw264.7 cells. Our results show that the expression level of Nitric Oxide (NO) and Matrix-metallopeptidase-9 (MMP-9) significantly decreased. Moreover, to determine the expression level of pro-inflammatory cytokines such as Tumor Necrosis Factor ($TNF-{\alpha}$) and Interleukin-6 (IL-6) and the phosphorylation pattern of signaling molecules of mitogen-activated protein kinase (MAPK) family, we performed ELISA and westren blot in Raw264.7 cells. In addition, nuclear factor-kappa B ($NF-{\kappa}B$) pathway was confirmed. PF extract inhibited the production of $TNF-{\alpha}$ and IL-6. The extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of $NF-{\kappa}B$ p65 in activated cells. Our results suggest that PF can be used as a potential therapeutic agent or functional food to relieve inflammation.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.18-25
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• 2014

This study was conducted to investigated effects of water relation of mulching and trickle irrigation on the external and internal fruit quality in Satsuma mandarin grafted on trifoliate orange rootstock in a orchard assigned to randomly three groups; whole period of Tyvex mulching (TM), Tyvex mulching with trickle irrigation once a week from October 22 to harvesting season (WM) and non-mulching treatment (NM). The average soil moisture content in the TM was lower than the WM during the time of trickle irrigation from Oct. 21 to Nov. 28. The leaf water potential was at the level of ${\Psi}max$ of -1.5 to -2.5 MPa during whole period of Tyvex mulching treatment but gradually increased at the point of supplement of water. The water and osmotic potential in juice vesicle was decreased by drought but increased again in response to the supply of water in WM. The total soluble solids (TSS) in fruit juice was increased by drought stress, but diminished in response to supply of water after drought. The content of titratible acidity was increased by drought stress but gradually decreased due to supplement of water after drought, reached it at the level of 1%. It was suggested that the accumulation of the total soluble solids compensates the degree of active osmoregulation and the decrease in content of acidity accounts for the fast respiration and water uptake resulted of the water after drought.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.45-50
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• 2005

Virus-free stocks was produced by the combination of the heat treatment of virus infected plant and shoot-tip grafting (STS). To produce virus-free stocks, the plants infected with citrus viruses were used for virus-free stock production using the modified method of STG in thermotherapy at $40^{\circ}C$ for 16 hours in the light, and at $30^{\circ}C$ for 8 hours of darkness for 4 weeks. Trifoliate orange (P. trifoliata) were used as rootstock seedling for STG. Percentages of virus-free stocks against citrus tristeza virus (CTV), satsuma dwarf virus (SDV) and citrus tatter leaf virus (CTLV) were $75.7\%,\;100.0,\%\;82.6\%$ respectively. Shoot tip size for successful STG were as small as possible. Less than $0.3\;\cal{mm}$ of shoot tips gave the hight efficiency of virus free plants but survival rates were low. And, survival rate after shoot-tip culture was analyzed and the rates were dependant on the cultivars; Yuzu cultivar showed the hight survival rate ($74.6\%$) and early satsuma mandarin (Iwasagi) was $13.3\%$ as the lowest cultivar. But citrus trees were not succeed to grown, turned brown, and died.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.162-167
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• 2018

The objective of this study was to find out a nomenclature and a code number for fruit commodities from the Codex Alimentarius Commission (Codex) corresponding with a commodity name used in South Korea. In addition, nomenclature or classification for commodity that needs an alteration or detailed examination domestically was determined. In this study, 'Food Code (Korean and English version)' and 'Pesticide MRLs in Food' from the Ministry of Food and Drug Safety and 'Codex Classification of Foods and Animal Feeds' were used. As results, regarding a nomenclature or classification used in South Korea, it appeared that alteration or further examination was needed for the following (English name of commodity, coming from an English version of Food Code). First, reconsiderations for classification of Chinese matrimony vine, fig, five-flavor magnolia vine, and pomegranate are needed as they are classified differently between Korea and Codex. Second, in any case of Korean or English language, nomenclature of commodity is different even within Korea or when it is compared with Codex. Such commodities are: Asian citron, Chinese bush cherry, Chinese matrimony vine, coconut, crimson glory vine, date palm, five-flavor magnolia vine, five-leaf chocolate vine, Japanese apricot, Japanese cornelian cherry, jujube, kiwifruit (golden kiwi), Korean black berry, Korean raspberry, kumquat, lychee, mandarin, persimmon, plum, quince, raspberry, and trifoliate orange. Third, reconsiderations for peach and raspberry nomenclatures are needed as it is currently unclear whether 'peach' includes nectarine and an English nomenclature, 'raspberry', is used in Korea for both various varieties (red, black) and one specific variety.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.311-322
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• 2003

Mycorrhizae is well known to increase the uptake of P and other mineral nutrients of plants. But if available P levels in soil is too low or high, mycorrhizae formation is limited. This study was carried out to determine the optimum level of available P for mycorrhizae formation of Poncirus trifoliata (trifoliate orange) seedling in volcanic ash soil. Eight levels of P in the range $0-1050mg\;kg^{-1}$ were applied with double superphosphate, and in each P level mycorrhizal fungi inoculated and uninnoculated treatments were included. The seedlings were grown in a greenhouse for 5 months and mycorrhizae formation, growth, and nutrient uptake were measured. As P application level increased, mycorrhizae formation increased at lower range of P application and the highest formation ratio of 43% was found at $100mg\;kg^{-1}$ P level ($2.6mg\;kg^{-1}$ available P in soil). At further higher levels of P application, mycorrhizae formation was rather suppressed. Seedling growth was increased by the inoculation of mycorrhizal fungi, and maximum growth was found at $100mg\;kg^{-1}$ P level where mycorrhizae formation was highest. The growth and mineral nutrient uptake of Poncirus trifoliata seedling represented a significant positive correlation with mycorrhizae formation at all P treatments.

• Journal of Life Science
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• v.23 no.2
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• pp.267-272
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• 2013

This study was conducted to eliminate viruses from citrus-infected plants using micrografting and thermotherapy. Six citrus cultivars including a 'Setoka' hybrid were used as plant sources. The TAS-ELISA technique demonstrated that several plants were CTV positive. However, no CTV symptoms were detected in plants obtained from shoots and treated at a high temperature of $40^{\circ}C$ during the day and night and micrografted for two weeks with old trifoliate orange rootstock in vitro. Indexing of CTV, SDV, and CTLV for RT-PCR analysis of the eleven citrus seedlings, including 'Setoka', 'Samdajosang', 'Pungkwang', 'Shiranuhi', and 'Ehimekashi dai28go' was virus free following the micrografting and thermal therapy.