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http://dx.doi.org/10.5352/JLS.2013.23.2.267

Micrografting and Heat Treatment Combination for Eliminating Virus of CTV-infected Citrus  

Chae, Chi Won (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Yun, Su Hyun (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Jae Ho (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Hyun, Jae Wook (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Koh, Sang Wook (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Dong Hoon (Planning and Coordination Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 267-272 More about this Journal
Abstract
This study was conducted to eliminate viruses from citrus-infected plants using micrografting and thermotherapy. Six citrus cultivars including a 'Setoka' hybrid were used as plant sources. The TAS-ELISA technique demonstrated that several plants were CTV positive. However, no CTV symptoms were detected in plants obtained from shoots and treated at a high temperature of $40^{\circ}C$ during the day and night and micrografted for two weeks with old trifoliate orange rootstock in vitro. Indexing of CTV, SDV, and CTLV for RT-PCR analysis of the eleven citrus seedlings, including 'Setoka', 'Samdajosang', 'Pungkwang', 'Shiranuhi', and 'Ehimekashi dai28go' was virus free following the micrografting and thermal therapy.
Keywords
Citrus; CTV; virus free plant; RT-PCR; TAS-ELISA;
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1 Ballester-Olmos, J. F., Pina, J. A., Carbonell, E. A., Moreno, P., Hermoso de Mendoza, A., Cambra, M. and Navarro, L. 1993. Biological diversity of citrus tristeza virus (CTV) isolates in Spain. Plant Pathol 42, 219-229.   DOI   ScienceOn
2 Gowda, S., Satyanarayana, T., Robertson, C. J., Garnsey, S. M. and Dawson, W. O. 2005. Infection of citrus plants with virions generated in Nicotiana benthamiana plants agroinfiltrated with binary vector based Citrus tristeza virus. pp. 23-33, In Hilf, M. E., Duran-Vila, N. and Rocha-Pena, M. A. (eds.), Proceedings of the 16th Conference of the International Organization of Citrus Virologists CA: IOCV Riverside.
3 Hilf, M. E. 2008. An immunocapture RT-PCR procedure using Apple stem grooving virus antibodies facilitates analysis of citrus tatter leaf virus from the original meyer lemon host. Plant Dis 92, 746-750.   DOI   ScienceOn
4 Iwanami, T. 2010. Properties and control of satsuma dwarf virus. Jpn Agric Res Q 44, 1-6.   DOI
5 Kano, T., Hiyama, T. Natsuaki, T., Imanishi, N., Okuda, S. and Ieki, H. 1998. Comparative sequence analysis of biologically distinct isolates of citrus tristeza virus in Japan. Ann. Phytopathol Soc Jpn 64, 270-275.   DOI
6 Kim, D. H. 2003. Identification and molecular characterization of citrus tristeza virus in Korea. Ph. D. thesis, SungKyun Kwan University, Seoul, Korea.
7 Kim, D. H., Oh, D. H., Hyun, C. W., Kwon, H. M., Kim, D. H. and Lee, S. C. 1999. Incidence of three major citrus viruses in Cheju Island. Plant Dis Agric 5, 34-40.   과학기술학회마을
8 Mary, E. H., Susan, D. L. Michael, M. and Kenneth, C. 1991. Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus. J Gen Virol 72, 1013-1020.   DOI   ScienceOn
9 Murashige, T., Bitters, W. P., Rangan, T. S., Nauer, E. M., Roistachek, C. N. and Holliday, P. B. 1972. A technique of shoot apex grafting and its utilization towards recovering virus-free citrus clones. Hort Sci 7, 118-119.
10 Murashige, T. and Skoog, R. 1962. Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473-497.   DOI
11 Navarro, L., Roistacher, C. N. and Murashige, T. 1975. Improvement of shoot-tip grafting in vitro for virus-free citrus. J Am Soc Hort Sci 100, 471-479.
12 Ohta, S., Kuniga, T., Nishikawa, F., Yamasaki, A., Endo, T., Iwanami, T. and Yoshioka, T. 2011. Evaluation of novel antiviral agents in the elimination of satsuma dwarf virus (SDV) by semi-micrografting in citrus. J Japan Soc Hor Sci 80, 145-149.   DOI
13 Raza, H., Khan, M. M. and Khan, A. A. 2003. Seedlessness in citrus. Int J Agri Biol 5, 388-391.
14 Shahsavar, A. and Khosh-khui, M. 1994. The effects of several variables on shoot-tip grafting of Clementine mandarin onto Troyer citrange. Iran Agric Res 13, 1-18.
15 Zarei, A. and Rahimian, H. 1997. Elimination of citrus tristeza virus from two cultivars of Satsuma mandarin through shoot-tip grafting. Iranian J Plant Pathol 33, 84-89.