• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.314-334
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• 2013

This study explored an efficient modified Quick, Easy, Cheap, Effective, Rugged and safe (QuEChERS) method combined with liquid chromatography-electrospray ionization with tandem mass spectrometric detection for the analysis of residues of 76 pesticides in brown rice, barley and corn including acidic sulfonylurea herbicides. Formic acid (1%) acid in acetonitrile and dispersive solid phase extractions used for extraction of pesticides and clean-up of the extract respectively. Two fortified spikes at 50 and 200 ng $g^{-1}$ levels were performed for recovery test. Mean recoveries of majority of pesticides at two spike levels ranged from 73.2 to 132.2, 80.9 to 136.8, 66.6 to 143.5 for brown rice, barley and corn respectively with standard error (CV) less than 10%. Good linearity of calibration curves were achieved with $R^2$ > 0.9907 within the observed concentration ranged. The modified method also provided satisfactory results for sulfonylurea herbicides. The method was applied to the determination of residues of target pesticides in real samples. A total of 26 pesticides in 36 out of 98 tasted samples were observed. The highest concentration was observed for tricyclazole at 1.17 mg $kg^{-1}$ in brown rice. This pesticide in two brown rice samples exceeded their MRLs regulated for rice in republic of Korea. Except tricyclazole none of the observed pesticides' concentration was higher than their MRLs. The results reveal that the method is effectively applicable to routine analysis of residues of target pesticides in brown rice, barley and corn.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.421-428
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• 2008

This study was carried out to elucidate the effects of adjuvants on physicochemical properties and pest control efficacy of pesticide spray solution. Spray droplet sizes of pesticide solution decreased by addition of adjuvants even though its effect varied according to the pesticides. The adhesive effect of pesticides sprayed on target crops by addition of adjuvants varied according to the properties, the application methods, and the spray volume of pesticides. Wash-off of the deposited amount of active ingredients of the solution of tricyclazole WP and fenobucarb EC by simulated rainfall after spraying on rice plants dramatically reduced by adding adjuvants. The retained amount of active ingredients of the pesticides were $1.5{\sim}4.1$ times higher than those without adjuvants when subjected to simulated rainfall 6 hours after spraying. Addition of adjuvants to the propanil EC spray solution enhanced the control efficacy in barnyard grass, Echinocloa crus-galli Beauv. by $8{\sim}30%$, which showed a potential to reduce pesticide use.

• Korean Journal of Environmental Agriculture
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• v.2 no.2
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• pp.108-113
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• 1983

There has been two kind of research fields in evaluating the ecotoxicity of chemicals The one is a bioassay and the other is an ecosystem analysis. The toxicants are transported into different biota, which have quite different environmental behaviour patterns and toxic properties. The effects of pesticides (butachlor, carbofuran, and tricyclazole) on periphyton community was studied by analyzing content of chlorophyll-a and autotrophic index (AI) that is a means of determining the trophic nature of the periphyton community. Results indicated that the content of chlorophyll-a was not influenced by the pesticides. The growth of algae was inhibited by sunshine period. And AI value is 43-2027: the large value indicates heterotrophic periphyton community, and was not affected by pesticides.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.27-31
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• 2001

Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

• Journal of Environmental Analysis, Health and Toxicology
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• v.21 no.4
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• pp.304-315
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• 2018

Located in Gimhae, Hwapo is the biggest riverine wetland in the province of Gyeongsangsam-do, Korea, and is a major habitat for various species. However, it is suspected that various pollutants enter the wetlands from agricultural and industrial areas. This study identifies major organic pollutants in this wetland and their sources using high performance liquid chromatography-high resolution mass spectrometry during one summer season. Forty-five substances were selected for quantitative analysis using target screening, and other non-selected compounds were screened using suspect and non-target screening methods. The results were that 21 and 17 targeted substances were detected in July and August, respectively. Major pollutants in July and August were oxadiazon (July: 17-220 ng/L, August: 66-460 ng/L), carbendazim (July: 10-110 ng/L, August: 64-520 ng/L), caffeine (July: 33-1,100 ng/L, August: 56-580 ng/L), and niflumic acid (July: 23-75 ng/L, August: 42-290 ng/L). Sampling sites S4 in July and S2 in August were the major inflow points. Ten substances (tricyclazole, hexaconazole, diuron, fexofenadine, irbesartan, simetryn, cimetidine, valsartan, tebuconazole, and benzotriazole) and four metabolites (valsartan acid, azoxystrobin acid, TEB_M324c, and 2-aminobenzimidazole) were tentatively identified through suspect and non-target screening, respectively.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.75-89
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• 2006

The actinomycete strain JK-1 that showed strong inhibitory activity against some plant pathogenic fungi and oomycetes was isolated from Jung-bal Mountain in Ko-yang, Korea. The strain JK-1 produced spores singly borne on sporophores and the spores were spherical and 0.9-1.2 11m in diameter. The cell wall of the strain JK-1 contained meso-diaminopimelic acid. The actinomycete strain JK-1 was identified as the genus Micromonospora based on the morphological, physiological, biochemical and chemotaxonomic characteristics. From the 168 rDNA analysis, the strain JK-1 was assigned to M aurantiaca. The antibiotic MA-1 was purified from the culture broth of M aurantiaca JK-1 using various purification procedures, such as Diaion HP20 chromatography, C18 flash column chromatography, silica gel flash column chromatography and Sephadex LH-20 column chromatography. $^{1}H-$, $^{13}C-NMR$ and EI mass spectral analysis of the antibiotic MA-1 revealed that the antibiotic MA-1 is identical to phenylacetic acid. Phenylacetic acid showed in vitro inhibitory effects against fungal and oomycete pathogens Alternaria mali, Botrytis cinerea, Magnaporthe grisea, Phytophthora capsici and yeast Saccharomyces cerevisiae at < 100 $\mug$ $ml^{-1}$. In addition, phenylacetic, acid completely inhibited the growth of Sclerotinia sclerotiorum, Bacillus subtilis, Candida albicans, Xanthomonas campestris pv. vesicatoria at < $\mug$ $ml^{-1}$. Phenylacetic acid strongly inhibited conidial germination and hyphal growth of M grisea and C. orbiculare. Phenylacetic acid showed significantly high levels of inhibitory' effect against rice blast and cucumber anthracnose diseases at 250 $\mug$ $ml^{-1}$. The control efficacies of phenylacetic acid against the two diseases were similar to those of commercial compounds tricyclazole, iprobenfos and chlorothalonil .n the greenhouse.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.230-237
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• 2007

This study was carried out to indicate the status of agricultural environment in Yangpyong area which is special protection area for tap water in Han river during three years($1996{\sim}1998$). The main study was on pesticide monitoring, analysis of pesticides in Bokpocheon water and soil. Water in this small river for supplying to the rice was found nine pesticides residues, ranged from 0.1 to 22.7 ppb, similar patterns from survey conducted in National Institute of Agricultural Science and technology, Soil in rice paddy has also low levels of eleven pesticide residues, ranged from 0.002 to 0.55 ppm.

• The Korean Journal of Pesticide Science
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• v.20 no.4
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• pp.312-318
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• 2016

This study investigated the reduction of residual pesticide in soil by using eight kinds of soil amendments such as shell, quick lime, dolomite, silicic acid, leaf mold, oil cake, bed soil and husk. A total of ten pesticides most frequently detected in agricultural soil were selected (azoxystrobin etc.) and soil amendments were treated in soils at a 2% application rate and incubated for 7 days. Pesticides were analyzed by HPLC-MS/MS with QuEChERS-based sample preparation procedure and pH was measured on 1, 3 and 7 days. This study showed that, soil amemdments were effective in reducing pesticides after 7days of incubation. Over 90% of azoxystrobin, cadusafos, chlorpyrifos, fluquinconazole, imidacloprid, isoprothiolane and procymidone were decomposed from soils amended with 2% quick lime, whereas the concentration of boscalid, dimethomorph and triycyclazole were not decreased. The soil pH increased to 12.8-12.9 in soil amended with quick lime, but other soil conditioners did not change the soil pH. Quick lime was particularly effective in reducing residual pesticides.

• The Korean Journal of Pesticide Science
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• v.4 no.4
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• pp.35-39
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• 2000

In order to develop the indicators of environmental impact of pesticide, its actual usage in paddy rice was surveyed, and usage trends of individual pesticides were evaluated. The tendency of pesticide use indicated insecticide 43%, herbicide 29%, fungicide 27% and top ranking item in insecticide, herbicide and fungicide was carbofuran, molinate + pyrazosulfuran-ethyl, IBP. The usage statistics of formulation types showed GR>DP>WP>EC>FG>SP. Pesticide usage(a.i.) per hectare was 7.13kg and total usage for paddy rice was estimated at 8,387 M/T. In the result of comparison of fact-usage with pesticide consumption reported in 1998, the fitness was 94.7% for fungicide, 84.3% for insecticide, 77.8% for herbicide. The result of monitoring pesticide residue of unpolished-rice sampled from farm house of survey indicated 0.14 ppm(BPMC), 0.16 ppm(Isoprocarb), 0.17 ppm(Isoprothiolane). In case of rice straw, the residue level was 0.27 ppm(Isoprothiolane), 0.28 ppm(IBP), 0.39 ppm(Carbofuran). The residue levels of pesticides were below MRLs.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.247-263
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• 2016

Safe animal feed is important for the health of animals, the environment and for the safety of foods of animal origin. Therefore, the aims of this study were: 1) to validate simultaneous analytical method and 2) to investigate the amount of pesticide residues in animal feed from Republic of Korea as a part of official control. A total of 126 samples were collected in 2015 and analyzed for 105 pesticides. According to pesticides monitoring result, no residue was found in 84.1% of the samples, whereas 15.9% of samples contained pesticide residues below the maximum residue limits (MRLs). Pirimiphos-methyl and cyproconazole were the two most frequently found pesticides. The results show that all commercial feed monitored in 2015 were safe under the Korean MRL and occurrence of pesticide residues in animal feed could not be considered serious threats to human and animal health. However, continuous monitoring with tighter regulation for pesticide residues in animal feed is recommended.