• Asian-Australasian Journal of Animal Sciences
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• v.29 no.5
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• pp.631-639
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• 2016

China has a long history of sheep (Ovis aries [O. aries]) breeding and an abundance of sheep genetic resources. Knowledge of the complete O. aries mitogenome should facilitate the study of the evolutionary history of the species. Therefore, the complete mitogenome of O. aries was sequenced and annotated. In order to characterize the mitogenomes of 3 Chinese sheep breeds (Altay sheep [AL], Shandong large-tailed sheep [SD], and small-tailed Hulun Buir sheep [sHL]), 19 sets of primers were employed to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) sequence of each breed. The sizes of the complete mitochondrial genomes of the sHL, AL, and SD breeds were 16,617 bp, 16,613 bp, and 16,613 bp, respectively. The mitochondrial genomes were deposited in the GenBank database with accession numbers KP702285 (AL sheep), KP981378 (SD sheep), and KP981380 (sHL sheep) respectively. The organization of the 3 analyzed sheep mitochondrial genomes was similar, with each consisting of 22 tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA), 13 protein-coding genes, and 1 control region (D-loop). The NADH dehydrogenase subunit 6 (ND6) and 8 tRNA genes were encoded on the light strand, whereas the rest of the mitochondrial genes were encoded on the heavy strand. The nucleotide skewness of the coding strands of the 3 analyzed mitogenomes was biased toward A and T. We constructed a phylogenetic tree using the complete mitogenomes of each type of sheep to allow us to understand the genetic relationships between Chinese breeds of O. aries and those developed and utilized in other countries. Our findings provide important information regarding the O. aries mitogenome and the evolutionary history of O. aries inside and outside China. In addition, our results provide a foundation for further exploration of the taxonomic status of O. aries.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.62-70
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• 2014

This study was carried out to analysis the vegetation status, the habitat size and the meaning of new natural habitat, and to provide basic data for conservation or management on the newly augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. A. distichum is a Korean monotypic endemic species. Most natural habitats of A. distichum have been reported in South-Central area around Chungcheong-do. The extent of occurrence of A. distichum can be enlarged to Central area due to the Yeoju-si habitat. The new habitat is located on a hillock which is composed of rock field at $37^{\circ}20{\sim}21^{\prime}N$, $137^{\circ}43{\sim}21^{\prime}E$, and 99~120m a.s.l.. The habitat size is small as about $530m^2$, and a small stream runs aside. The number of individuals of A. distichum was about 1,200. But most individuals were smaller than 0.5m height, so just about 300 individuals are taller than 1.0m height. A. distichum on Yeoju-si seemed to be maintained and distributed by vegetative propagation from elongated roots of branches. Pinus rigida and Quercus aliena dominated tree layer, and Lindera obtusiloba, Q. aliena and Acer tatricum appeared frequently in subtree layer. The shrub layer was dominated by A. distichum with Stephanandra incisa, Euonymus alatus for. ciliatodentatus, Ligustrum obtusifolium, etc. The coverage of herbaceous layer was low.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.111-117
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• 1997

Occurrence status of Tetranychus viennensis Zacher was investigated in Kyonghuk province. Its ecological studies were carried out and chemical control efficacy against females and eggs was examined. And the results ohtained are as follows. T. viennensis occurred from mid April through mid November with the peak time in June on cherry tree. Population level of T. viennensis was varied from 0 to 160 individuals per 100 peach leaves in different peach orchards, and its occurrence rate was 35% out of 20 ochards. However no T. viennensis was observed from 25 apple orchards in 5 districts of Kyongbuk province. Overwintering stage of T. viennensis females appeared in mid October, and most of them moved into under the rough hark in early November. The overwintered adult females moved out from the rough bark to leaves in early April. Mortality during overwintering was about 55%. Under four constant temperatures of 16, 19, 22, 25$^{\circ}$C. egg periods were 14. 3, 9.2, 6.8, and 4.0 days ; larva -nymph periods were 20.2, 13.9, 8.7, and 6.6 days. Female longevity was 67. 7, 60.7, 46.4, and 34.0 days : mean tota: fecundities per female were 21.2, 44.3, 54.4, and 64.7 eggs. Some acaricides, azocyclotin, pyridaben, propargite, tehufenpyrad and fenpyroximate were highly effective against females, while hexythiazox and clofentezine showed very low control effect from 3.6 to 14.4%. Whereas, all of 7 acaricides tested highly effective against the eggs.

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.179-188
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• 2020

Weeding refers to the process of removing weeds around crop trees, which cause competition in nutrients, moisture, and light, and has a great effect on the early growth and survival of crop trees for establishing forest resources. This study was conducted to find out the timing and method of weeding that is the most suitable for the major afforestation species in national forests. We investigated (1) weeding time and frequency for each afforestation species and (2) the height of crop trees and the length of weeds. Up to 99% weeding operation was carried out until 5 years following afforestation. Pinus densiflora, Larix kaempferi, Chamaecyparis obtusa, Prunus sargentii, and Betula platyphylla plantations accounted for more than 70% of the total weeding sites. Once-a-year weeding process was conducted from late June to late July (67%), and twice-a-year weeding process was the highest in June-August by 31%. Most species were shorter than the weeds until the first and second year of planting, but the height of the crop trees was higher than that of the weeds by 48% in average from the third year. Therefore, from the third year of afforestation, except for some species, it is possible to reduce the cost of weeding operations while reducing nutrient competition by the weeds through the removal of weed sprouts, mainly woody weeds, rather than clear cutting. This study contributes to efficiently develop forest resource establishment while reducing operational costs through a detailed weeding schedule and species-specific method.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.326-335
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• 2015

Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.

• The Korean Journal of Mycology
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• v.32 no.2
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• pp.152-157
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• 2004

In this study the ITS1, ITS2 and 5.8S ribosomal DNA sequences from 29 strains of the Genus Inonotus and its related genera were compared with 31 strains obtained from GenBank database. Using the neighbor-joining (NJ) method and most parsimonious analysis the phylogenetic tree was constructed. The hymenochaetales formed no monophyletic group and several non-hymenochaetales appeared as intermingled with the Hymenochaetales. Strains 6, 46, 49, 50, 53, 55 showed no certain affinities within the Hymenochaetales, whereas Inonotus sp. (51) was closely related to Phellinus baumii, and Inonotus sp. (52), and Inonotus glomeratus (10) was related to Phellinus linteus, and Fomes fomentarius (30) was related to Ganoderma lucidum. Inonotus sp. and Phellinus sp. formed no monophyletic groups and a subdivision in the following genera is accepted: Inonotus sp. Phellinus baumii, Phellinus linteus, Phellinus igniarius, Phellinus pini, Hericium erinaceum, Ganoderma lucidum and Sparassis sp. were confirmed and separated genera. The taxonomic status of Inonotus remained uncertain. Eight new combinations are proposed.

• The Journal of Internal Korean Medicine
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• v.32 no.1
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• pp.129-135
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• 2011

This report documents a case in which the administration of an herbal product, an extract of the lacquer tree, Rhus verniciflua Stokes, as sequential and concurrent treatment with chemotherapy was associated with a long term survival and good quality of life in a patient with metastatic non-small cell lung cancer(NSCLC). A 51-year-old Korean female was referred to the $M{\cdot}{\mu}$ Integrative Cancer Center, East-West Neo Medical centrer, Kyung Hee University for stage IV, metastatic NSCLC. She was treated with aRVS alone for 19 months and then received 1st line paclitaxel/carboplatin combined with aRVS, 2nd line gefitinib, and 3rd line pemetrexed. The number of cycles of pemetrexed administered was seventeen. aRVS was restarted as the 13th pemetrexed was administered. Pemetrexed with aRVS is currently ongoing. This patient has been alive for 41 months, and has been keeping a good performance status so far. We suggest aRVS as sequential and concurrent treatment with chemotherapy is an effective alternative treatment strategy.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.4
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• pp.348-357
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• 2015

The main purpose of this study is to understand the potential geographic distribution of P. koraiensis, which is known to be one of major economic tree species, based on the RCP (Representative Concentration Pathway) 8.5 scenarios and current geographic distribution from National Forest Inventory(NFI) data using ecological niche modeling. P. koraiensis abundance data extracted from NFI were utilized to estimate current geographic distribution. Also, GARP (Genetic Algorithm for Rule-set Production) model, one of the ecological niche models, was applied to estimate potential geographic distribution and to project future changes. Environmental explanatory variables showing Area Under Curve (AUC) value bigger than 0.6 were selected and constructed into the final model by running the model for each of the 27 variables. The results of the model validation which was performed based on confusion matrix statistics, showed quite high suitability. Currently P. koraiensis is distributed widely from 300m to 1,200m in altitude and from south to north as a result of national greening project in 1970s although major populations are found in elevated and northern area. The results of this study were successful in showing the current distribution of P. koraiensis and projecting their future changes. Future model for P. koraiensis suggest large areas predicted under current climate conditions may be contracted by 2090s showing dramatic habitat loss. Considering the increasing status of atmospheric $CO_2$ and air temperature in Korea, P. koraiensis seems to experience the significant decrease of potential distribution range in the future. The final model in this study may be used to identify climate change impacts on distribution of P. koraiensis in Korea, and a deeper understanding of its correlation may be helpful when planning afforestation strategies.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.189-207
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• 2006

Goodyera rosulacea, which is morphologically similar to G. repens, is described recently as a new species based on its distinct morphological characters such as rosette-formed leaves, short rhizome and habitat. To verify the taxonomic identity of G. rosulacea and its taxonomic relationship within Korean Goodyera taxa, sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA and the trnL region of cpDNA from 24 accessions including 1 outgroup accession were analyzed. Aligned sequences were analyzed using maximum parsimony and distance method, and the taxonomic identity and the taxonomic relationships among the related taxa were estimated by the existence of private marker gene and the phylogenetic tree of the aligned sequences. Molecular data indicate that G. rosulacea gas several private marker genes and shows monophyly in phylogenetic trees of both ITS and trnL sequences. the pairwise distance between G. rosulacea and the orher taxa of Korean Goodyera was 3.49-6.68% for ITS region and 5.05-9.53% for trnL region, indicating that G. rosulacea could be treated as an independent species. Therefore, our molecular data support the taxonomic of G. rosulacea as a distinct species of Korea. In phylogenetic trees, G. rosulacea formed same clade with G. repens, which has similar morphological characters with G. rosulacea, and showed the lowest pairwise distance with G. repens among Korean Goodyera taxa. These molecular data sugguested that G. rosulacea and G. repens are closely related taxa.

• Journal of Forest and Environmental Science
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• v.30 no.4
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• pp.383-392
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• 2014

In this study, we investigated the Fukiage sand dunes of southern Kyushu, Japan. We surveyed the status of recovery of coastal Japanese black pine forests damaged by pine wilt disease and their disaster prevention capability. We placed two transects: Transect 1, in an area that was severely damaged (80-90% damage rate) by pine wilt disease, and Transect 2, in an area that was mostly undamaged (<10% damage rate). Then, we installed survey lines, carried out vegetation surveys, and measured the depth and pH of humus soil. The survey lines were placed perpendicular to the coastline from the top of the fore-dune to the inland area, and divided into five 50 m sections. Before the point 100 m inland from the top of the fore-dune, the number of invasive hardwoods and of Japanese black pines were small because of the poor growth environment in both transects. Past the 100 m point, the species and number of Japanese black pines and broad-leaved trees increased further inland because the growth environment improved. In addition, the recovery metrics of tree height, diameter at breast height, age, and number in Transect 1 were much lower than those in Transect 2, and the basal area of broad-leaved trees and the depth of humus soil in Transect 1 were lower than in Transect 2, and the soil pH of humus soil in Transect 1 was higher than that of Transect 2. The shape ratio of the Japanese black pine forests indicated that they were insufficient for disaster prevention. Therefore, in order to fully promote the disaster prevention capability of coastal Japanese black pine forests, we should not only focus on prevention of pine wilt disease but also undertake continuous control efforts taking into consideration the sound growth environment such as appropriate density and soil management and removal of invasive broad-leaved trees.