Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Taxonomic status of Goodyera rosulacea (Orchidaceae): molecular evidence based on ITS and trnL sequences

로젯사철란(Goodyera rosulacea: Orchidaceae)의 분류학적 위치: ITS와 trnL 염기서열에 의한 분자적 증거

  • Lee, Chang Shook (Division of Molecular & Life Sciences, Collage of National Sciences, Ewha Womens University) ;
  • Eom, Sang Mi (Division of Molecular & Life Sciences, Collage of National Sciences, Ewha Womens University) ;
  • Lee, Nam Sook (Division of Molecular & Life Sciences, Collage of National Sciences, Ewha Womens University)
  • 이창숙 (이화여자대학교 자연과학대학 생명과학과) ;
  • 엄상미 (이화여자대학교 자연과학대학 생명과학과) ;
  • 이남숙 (이화여자대학교 자연과학대학 생명과학과)
  • Published : 2006.09.30
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Abstract

Goodyera rosulacea, which is morphologically similar to G. repens, is described recently as a new species based on its distinct morphological characters such as rosette-formed leaves, short rhizome and habitat. To verify the taxonomic identity of G. rosulacea and its taxonomic relationship within Korean Goodyera taxa, sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA and the trnL region of cpDNA from 24 accessions including 1 outgroup accession were analyzed. Aligned sequences were analyzed using maximum parsimony and distance method, and the taxonomic identity and the taxonomic relationships among the related taxa were estimated by the existence of private marker gene and the phylogenetic tree of the aligned sequences. Molecular data indicate that G. rosulacea gas several private marker genes and shows monophyly in phylogenetic trees of both ITS and trnL sequences. the pairwise distance between G. rosulacea and the orher taxa of Korean Goodyera was 3.49-6.68% for ITS region and 5.05-9.53% for trnL region, indicating that G. rosulacea could be treated as an independent species. Therefore, our molecular data support the taxonomic of G. rosulacea as a distinct species of Korea. In phylogenetic trees, G. rosulacea formed same clade with G. repens, which has similar morphological characters with G. rosulacea, and showed the lowest pairwise distance with G. repens among Korean Goodyera taxa. These molecular data sugguested that G. rosulacea and G. repens are closely related taxa.

로젯사철란(G. rosulacea Y. Lee)은 애기사철란과 유사하나 로젯트형의 잎, 짧은 땅속줄기와 서식지 등의 특징에 의해 한국산 사철란속(Goodyera R. Br.) 내의 신종으로 기재된 바 있다. 로젯사철란의 분류군의 실체와 근연종간의 유연관계를 파악하기 위하여 군외군을 포함한 24개의 사철난속 식물을 대상으로 핵 리보좀(ribosomal)의 DNA internal transcribed spacer와 엽록체 DNA의 trnL 구간의 염기서열을 분석하였다. 분류군의 실체와 근연종간의 유연관계는 정렬된 염기서열을 바탕으로 최대절약분석(Maximum parsimony analysis)와 근연결합법(Neibour Joining method)에 의한 계통수 및 고유 표지유전자 여부로 추정하였다. 분석 결과 로젯사철란은 다수의 고유한 표지유전자를 가지며, ITS와 trnL 계통수에서 모두 단계통군을 형성하였다. 로젯사철란과 한국산 사철란속 내 각 분류군간의 유전적 거리(pairwise distance)는 ITS에서 3.49-6.68, trnL에서 5.05-9.53으로서 독립된 종으로 간주하기에 무리가 없었다. 따라서 분자적 결과는 로젯사철란을 사철란속내 독립된 종으로 처리하는 것을 지지하였다. 계통수에서 로젯사철란은 형태적으로 유사한 애기사철란(G. repens)과 동일한 분계조를 형성하였고, 유전적 거리도 조사한 분류군들 중 가장 낮은 값을 나타냈으므로 가장 가까운 근연분류군임을 나타내었다.

Keywords

Acknowledgement

Supported by : 환경부

References

  1. Baldwin, B. G., M. J. Sanderson, J. M. Porter, M. F. Wojciechowski, C. S. Camoell, and M. J. Donoghue. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri Bot. Gard. 82: 247 -277 https://doi.org/10.2307/2399880
  2. Bogler D. J. and B. B. Simpson. 1996. Phylogeny of Agavaceae based on ITS rDNA sequence variation. Amer. J. Bot. 83: 1225-1235 https://doi.org/10.2307/2446206
  3. Compton, J. A. and A. Culham. 2002. Phylogeny and circumscription of tribe Actaeeae Ranunculaceae. Sys. Bot. 27: 502-511
  4. Corriveau, J. L. and A. W. Coleman. 1988. Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. Amer. J. Bot. 75: 1443-1458 https://doi.org/10.2307/2444695
  5. Cronn, Rand J. F. Wendel. 2003. Cryptic trysts, genomic mergers, and plant speciation. New Phytologist. 161: 133-142 https://doi.org/10.1111/j.1469-8137.2004.00947.x
  6. Chung, T. H. 1956. Korean Flora II. Shinzisa, Seoul. Pp. 1009-1010. [In Korean]
  7. Downie, S. Rand D. S. Katz-Downie. 1996. A molecular phylogeny of Apiaceae subfamily Apioideae: evidence from nuclear ribosomal DNA internal transcribed spacer sequences. Amer. J. Bot. 83: 234-251 https://doi.org/10.2307/2445943
  8. Downie, S. Rand D. S. Katz-Downie. and K. Spalik. 2000. A phylogeny of Apiaceae tribe Scandiceae evidence from nuclear ribosomal DNA internal transcribed spacer sequences. Amer. J. Bot. 87: 76-95 https://doi.org/10.2307/2656687
  9. Farris, J. S., M. Kallersjo, A. G. Kluge and C. Bult. 1995. Testing significance of incongruence. Cladistics, 10: 315-319 https://doi.org/10.1111/j.1096-0031.1994.tb00181.x
  10. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791 https://doi.org/10.2307/2408678
  11. Freudenstein J. V. 2001. A Systematic Study of Endothecial Thickenings In the Orchidaceae. Amer. J. Bot. 78: 766-781 https://doi.org/10.2307/2445067
  12. Gibson, T., D. Higgins and J. Thompson. 1994. Clustal X Program. EMBL, Heidelberg, Germany
  13. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol.: 11-120
  14. Lee, C. S., N. S. Lee and S. H. Yeau. 2004. Molecular phylogenetic relationships and speciation of Ranunculus cantoniensis (Ranunculaceae), Korean J. PI. Taxon. 34: 335-358. [In Korean] https://doi.org/10.11110/kjpt.2004.34.4.335
  15. Lee, C. S. and S. R. Downie. 2006. Phylogenetic relationships within Cicuta (Apiaceae trbe Oenantheae) inferred from nuclear rDNA ITS and cpDNA sequence data. Canad. J. of Bot. 83: 453-468
  16. Lee, Y. 1996. Flora of Korea. Gyohaksa, Seoul. [In Korean]
  17. Lee, Y. 2004. I. New Taxa of Korean Flora. Bulletin of Koran Plant Research. 4: 2-4. [In Korean]
  18. Li, H. L. 1978. Flora of Taiwan. vol. 5. Epoch Pub. Co., Taipei, Taiwan. Pp. 1006-1019
  19. Nakai, T. 1911. Flora Koreana II. J. ColI. Sci. Imp. Univ. 31: 226-227
  20. Qiu, Y-L., C. R. Parks and M. W. Chase. 1995. Molecular divergence In the Eastern Asia-Eastern North America disjunct section Rytidospermum of Magnolia (Magnoliaceae), Amer. J. Bot. 82: 1589-1598 https://doi.org/10.2307/2446188
  21. Saitou, N. and M. Nei. 1987. The Neighbour-joining method: A new method for reconstructing phylogenetic trees. Molec. Biol. Evol. 4: 406-425
  22. Satake, Y. J., S. Ohwi, S. Kitamura, S. Watari and T. Tominari. 1982. Wild Flowers of Japan. Monoco. [1]. Heibonsha, Tokyo
  23. Sim, J-K., I-I-D. Park and S-J. Park. 2002. Phylogenetic study of Korean Iris (Iridaceae) based on nrDNA ITS sequeces. Korean J. PI. Taxon. 32: 33-53 https://doi.org/10.11110/kjpt.2002.32.1.033
  24. Shin, K-S., Y. K. Shin, J-W. Kim and K-H. Tae. 2003. Phylogeny of the Genus Goodyera(Orchidaceae; Cranichideae) in Korea Based on Nuclear Ribosomal DNA ITS Region Sequences. J. Plant BioI. 45: 182-187
  25. Simmons, M. P. and H. Ochoterena. 2000. Gaps as Characters in Sequence-Based hylogenetic Analyses. Syst. Bot. 49: 369-381
  26. Swofford, D. L. 2002. PAUP*. Phylogenetic analysis USing parsimony (*and other methods), Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts
  27. Tae, K. H., E. H. Lee and S. C. Ko. 1997. A systematic study of the genus Goodyera in Korea by morphological and cytological characters. Korean J. PI. Taxon 27: 89-116. [In Korean] https://doi.org/10.11110/kjpt.1997.27.1.089
  28. Tae, K. H. and S. C. Ko. 1999. Interspecific relationship of the genus Goodyera in Korea using cluster analysis. Korean J. PI. Taxon 29: 63-73. [In Korean] https://doi.org/10.11110/kjpt.1999.29.1.063
  29. Taberlet, P., L. Gielly, G. Pautou, and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. PI. Molec. Biol. 17: 1105-1109 https://doi.org/10.1007/BF00037152
  30. Tsai, C. 2003. Molecular phylogeny, biogeography, and evolutinary trends of the genus phalaenopsis (Orchidaceae). Ph. D. thesis in Univ. of National Sun Yat-sen, Kaohsiung, Taiwan, Pp. 224
  31. White, T. J., T. Bruns, S. Lee, and J, Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp, 315-322. in PCR Protocols: A guide to methods and applications, eds. Innis, M. A., Gelfand, D. H., Sninsky, J, J., & White, T. J.. Academic Press, San Diego
  32. Yoo, K., S. Jang and W. Lee, 2005. Phylogeny of Korean Viola based on ITS sequeces. Korean J. PI. Taxon. 35: 7-23. [In Korean] https://doi.org/10.11110/kjpt.2005.35.1.007