• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.17-20
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• 1999

In rice, limited efforts have been made to identify genes by the use of insertional mutagens, especially heterologous transposons such as the maize Ac/Ds. We constructed Ac and gene trap Ds vectors and introduced them into the rice genome by Agrobacterium-mediated transformation. In this report, rice plants that contained single and simple insertions of T-DNA were analyzed in order to evaluate the gene-tagging efficiency. The 3'end of Ds was examined for putative splicing donor sites. As observed in maize, three splice donor sites were identified at the 3'end of the Ds in rice. Nearly 80% of Ds elements wered excised from the original T-DNA sites, when Ac cDNA was expressed under a CaMV 35S promoter. Repetitive ratoon culturing was performed to induce new transpositions of Ds in new plants derived from cuttings. About 30% of the plants carried at least one Ds that underwent secondary transposition in the later cultures. 8% of transposed Ds elements expressed GUS in various tissues of rice panicles. With cloned DNA adjacent to Ds, the genomic complexities of the insertion sites were examined by Southern hybridization. Half of the Ds insertion sites showed simple hybriodization patterns which could be easily utilized to locate the Ds. Our data demonstrate that the Ac/Ds mediated gene trap system could prove an excellent tool for the analysis of functions of genes in rice. We discuss genetic strategies that could be employed in a largee scale mutagenesis using a heterologous Ac/Ds family in rice.

• 한국환경과학회지
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• 제1권1호
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• pp.41-46
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• 1992

주거지역에서 대기오염물 시료채취에 대한 장기간 연구를 수행하기 위해서 휘발성 유기 화합물 분석에 적합한 24시간동안의 종합적인 공기시료를 채취하기 위해 공기시료 채취기가 제작되었다. 이 시료 채취기는 시료채취시 요구되는 모든 특성을 포함할 뿐만 아니라, 일반가정에 근접해서 설치되어야하므로 심미적 특성도 포함한다. 이 공기시료 채취기는 흡착제(티넥스)로 채워진 5/8인치 외경의 스테인레스 스틸 트랩 네개를 수용할수 있고, 분당 5-50 밀리리터의 범위에서 네 종류의 공기유속을 이용할수 있게 고안 되었다. 금속필도(10마이크로미터)가 트랩 어댑터의 입구에 직접 부착되었다. 부가적인 특성은 다음과 같다. : 1) 유기물질에 불활성인 재료로 제작 되었고, 2) 날씨에 영향을 받지 않고, 3) 전지를 이용하고, 4) 사람의 호흡영역에서 공기 시료를 채취하고, 그리고 5) 방음제로 싸여진 작은 펌프를 이용하여 조용하게 작동된다. 펌프/전지 장치는 시료채취기의 본체로부터 분리 되었다. 이 장치로 부터 야기되는 소음수준은 주거지역의 허용기준치(뉴저지주 환경보호국 기준)보다 낮았다. 이 공기시료 채취기는 일상 주거지역과 단층의 한 국민학교 옥상에서 성공적으로 작동되었다.

• 한국가시화정보학회지
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• 제21권3호
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• pp.65-74
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• 2023

Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

• 한국자동차공학회논문집
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• 제24권2호
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• pp.152-160
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• 2016

The market demand for diesel engine tends to increase in general passenger cars as well as commercial vehicles because of its advantages. However, to meet the vehicle emissions regulation which will be more stringent in the future, it is necessary to plurally apply all after-treatment technologies such as diesel oxidation catalyst (DOC), catalyzed diesel particulate filter (CDPF), lean NOx trap (LNT) and selective catalytic reduction (SCR), and so on. Accordingly, the exhaust after-treatment system for diesel vehicle requires the technology of minimizing the numbers of catalysts by integrating every individual catalysts. The purposes of this study is to develop hybrid exhaust after-treatment device system which simultaneously uses LNT/DPF and SCR/DPF catalyst concurrently reducing NOx and particulate matter (PM). As the results, the hybrid system with $NH_3$ generated at LNT/DPF working as a reducing agent of SCR/DPF catalyst, improving NOx conversion rate, was found to be more excellent in de-NOx performance than that in LNT/DPF alone system.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.57-60
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• 2008

KEPRI has studied planar type SOFC stacks using anode-supported single cells and kW class co-generation systems for residential power generation. A 1kW class SOFC system consisted of a hot box part, a cold BOP part and a water reservoir. A hot box part contains a SOFC stack made up of 48 single cells and ferritic stainless steel interconnectors, a fuel reformer, a catalytic combustor and heat exchangers. Thermal management and insulation system were especially designed for self-sustainable operation. A cold BOP part was composed of blowers, pumps, a water trap and system control units. When a 1kW class SOFC system was operated at $750^{\circ}C$ with hydrogen after pre-treatment process, the stack power was 1.2kW at 30 A and 1.6kW at 50A. Turning off an electric furnace, the SOFC system was operated using hydrogen and city gas without any external heat source. Under self-sustainable operation conditions, the stack power was about 1.3kW with hydrogen and 1.2kW with city gas respectively. The system also recuperated heat of about 1.1kW by making hot water.

• 한국수소및신에너지학회논문집
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• 제21권5호
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• pp.405-411
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• 2010

A 5kW class SOFC cogeneration system consisted of a hot box part, a cold BOP (balance of plant) part, and a hot water reservoir. The hot box part contained a stack, a fuel reformer, a catalytic combustor, and heat exchangers. A cold BOP part was composed of blowers, pumps, a water trap, and system control units. A 5kW stack was designed to integrate 2 sub-modules. In this paper, the 5kW class SOFC system was operated using 2 short stacks connected in parallel to test the sub-module and the system. A short stack had 15 cells with $15{\times}15 cm^2$ area. When a natural gas was used, the total power was about 1.38 kW at 120A. Because the sub-modules were connected in parallel and current was loaded using a DC load, voltages of sub-modules were same and the currents were distributed according to the resistance of sub-modules. The voltage of the first stack was 11.46 V at 61A and the voltage of the second stack was 11.49V at 59A.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 추계학술대회 논문집
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• pp.13-13
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• 2009

본 연구는 MLCC 구성성분의 증발/비등에 의한 저분자물질의 제거가 bar 수축에 미치는 영향도를 평가하기 위해, 압착 bar에서 발생하는 고농도의 out gas를 정량하기 위한 최적 system을 구축하고자 하였다. gas 포집에 범용적으로 사용하는 Purge & Trap sampler 대신 Heating block를 사용하여 gas를 발생시키고 동시에 solvent에 용해시킴으로써 고농도의 시료가 희석될 수 있는 전처리 장치를 디자인하였다. 그 결과 고농도 gas 주입에 의한 장비오염과 peak saturation 문제가 해결되었고, gas phase의 시료를 liquid phase로 상전이 시켜 autosampler를 이용한 정확한 량의 시료 주입이 가능하였다. System의 Gage Linearity와 Bias는 각각 1.7%와 1.3%로 개선이 필요없는 수준의 정확도를 가졌다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집D
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• pp.802-806
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• 2001

Diesel particulate trap is the most reliable system to reduce the particulate matters from diesel engine. Filter is the core component of DPF and ceramic monolith type is dominantly used, which is expensive and fragile relatively at thermal shock. Porous metal filter, which has superior thermal characteristics and low cost, was tested in order to analyze the regeneration performance by using with ferrocene additive. This filter showed the 72% filtration efficiency, additives itself diminished 48% of PM from engine out emission, and final PM reduction ratio of 89% was achieved by DPF system with D-13 test mode.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.41-45
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• 1992

Shikonin production was examined in a bubble column bioreactor system with the hairy roots of Lithosphermum erythrorhizon. The volumetric productivity was higher than those obtained from other reactor configurations with free or immobilized cells of the same cell line. The productivities of the bubble column reactor, with and without a product absorption trap, were 7.4 and 4.5 mg of shikonin/l/d, respectively. This indicated the importance of the product removal in the design and operation of the shikonin production system with hairy root culture.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(1)
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• pp.109-112
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• 2002

SNMP is generally used protocol for network management. SNMP need MIB for exchanging management information But, method for sharing MIB is not automatic. General MIB sharing architecture is done by network management operator by manually. In this paper we describe exchanging MIB automatically using meta-MIB. We will formalize SMI as MIB structure. This meta MIB represent information about MIB of NE Agent. Meta MIB has Information of object in the MIB, Structure and index information fur columnar object, meaning of subtype at INTEGER syntax. Also, trap related information is represented too. In this paper, MIB information is represented as instance of meta MIB. This architecture will provide a method for automatic MIB exchanging