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Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets

  • Su, Weipeng;Xu, Wen;Zhang, Hao;Ying, Zhixiong;Zhou, Le;Zhang, Lili;Wang, Tian
    • Nutrition Research and Practice
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    • v.11 no.2
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    • pp.121-129
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    • 2017
  • BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets. MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a $2{\times}2$ factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication. RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), ${\alpha}$-ketoglutarate dehydrogenase (${\alpha}$-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-${\gamma}$ coactivator $1{\alpha}$ (PGC-$1{\alpha}$). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, ${\alpha}$-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-$1{\alpha}$, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, $H^+$ transporting, mitochondrial F1 complex, ${\beta}$ polypeptide in liver. CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.

A Study on the Microbiological Analysis of BACCP in Hamburger (햄버거 제품에 대한 미생물학적 위해 요인 분석에 관한 연구)

  • 정일형;노완섭
    • The Korean Journal of Food And Nutrition
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    • v.14 no.5
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    • pp.467-478
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    • 2001
  • This study was focused on the sanitary analysis of hazard factors and the establishment of critical control points on hamburger by the microbiological investigation. The degree of microorganic pollution on the ingredients and equipments for hamburger manufacturing and the variation of microorganisms at storage time and temperatures were investigated. The magnitudes of total aerobic bacteria In hamburger were highly detected to be in the order of resting placed in expressway > convenience stores > fast food stores, and coliforms were lowly detected as convenience stores > fast food stores > resting places in expressway. In investigation of basic ingredients, the degree of microorganic pollution showed highly on patty. cabbage and cucumber. In investigated result of mocroorganic distribution at the various phases in hamburger manufacturing, total aerobic bacteria counts were detected over 5.5$\times$10$^2$ CFU/g, and coliforms counts were detected over 2.0$\times$10$^2$ CFU/g. In investigated result of microorganic distribution on the instruments and equipments for hamburger manugacturing, total aerobic bacteria counts were detected over 10$\^$5/ CFU/100cm2 and coliforms counts were detected over 10$^2$CFU/100㎠. Staphylococcus aureus was detected at wagon and refrigerator. Salmonella spp. was detected at grinder and Vibrio parahaemolyticus was not detected. At various storage temperatures, total aerobic bacteria counts insreased 3.0$\times$10$^3$ CFU/g to 7.0$\times$10$^4$ CFU/g, 4.2$\times$10$\^$7/ CFU/g and 8.1$\times$10$\^$8/ CFU/g at 10$\^{C}$, 20$\^{C}$, 30$\^{C}$ after 48 hours respectively. coliform counts also increased 4.5$\times$10$^2$ CFU/g to 2.2$\times$10$^3$ CFU/g, 5.4$\times$10$\^$5/ CFU/g, 4.5$\times$10$\^$6/ CFU/g at 10$\^{C}$, 20$\^{C}$, 30$\^{C}$ after 48 hours respectively. The establishment of critical control point CCP was divided into CCP1 and CCP2 by the removing level of hazard factor, and then CCP1 was established on basic ingredients, and CCP2 was established on the phases of mixing, pouring, packaging, transporting and preserving.

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${SO_4}^{2-}$ ion Removal from Solution by Crystal Precipitation on Surface Active Glasses (표면활성유리에의 결정석출을 이용한 용액중 황산염 이온 제거)

  • 남명식;김철영
    • Journal of the Korean Ceramic Society
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    • v.35 no.12
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    • pp.1286-1293
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    • 1998
  • ${SO_4}^{2-}$ ions present in industrial waste water if combined with other metal ions in the water can cause serious scale problem in a transporting pipe. In this study therefore ${SO_4}^{2-}$ ions in an acidic solution have been removed by using surface active glasses. Glasses with various compositions of $SiO_2-Na_2O-B_2O_3-RO$ (R=Mg, Ca, Sr, Ba) system were reacted in a ${SO_4}^{2-}$ ion-containing solution with various pHs ranging from 1 to 4 for various time the reacted glass surfaces were analyzed by XRD and SEM and all ions in the reacted solution were also measured ${SO_4}^{2-}$ ions in the solution were combined with divalent ions leached out of glass and precipitated on the glass surface as sulfate crystals. In this was the surface ion could be removed from the acidic solution. The sulfate ion removal capacity is closely related to the solubility product con-stants of the newly formed sulfate crystals. Almost no sulfate crystal was formed on the MgO-containing glass while sulfate crystals were easily formed on the glass containing either SrO or BaO This indicates that those glasses have strong removal efficiency of ${SO_4}^{2-}$ ions from the solution.

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A Study on the Habitat Environment and Mutualism with Ants of Genus Melampyrum - focusing on M. roseum, M. setaceum var. nakaianum and M. roseum var. ovalifolium in Kangwondo - (며느리밥풀속 서식지 특성 및 개미와의 공생관계에 관한 연구 - 강원도의 꽃며느리밥풀, 새며느리밥풀 및 알며느리밥풀을 중심으로 -)

  • Lee, Jae-Hyeon;Lyu, Dong-Pyo;Kim, Gab-Tae
    • Korean Journal of Environment and Ecology
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    • v.30 no.2
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    • pp.139-145
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    • 2016
  • To search for habitat characteristics of myrmecochory Melampyrum species, diaspores and related ant species, habitat characteristics, collecting fruits and diaspores of three Melampyrum species, cafeteria experiments, and the related ant species were studied from May 2012 to November 2014. The habitat of Melampyrum species was south-facing open forest edge with slightly acidic soil and relatively shallow soiil layers. Major plants coexisting with Melampyrum species were four woody species including Quercus mongolica and four herbaceous species including Carex siderosticta. These Plants might be host plants of the Melampyrum species. The shape of the diaspore was an oval seed attached to the white cap on one side. Significant differences is proved between three plant species in weight of diaspores, seeds and and elaiosome ratios. Mean values of diaspores weight showed 10.6mg, 8.0mg and 7.2mg in M. setaceum var. nakaianum, M. roseum var. ovalifolium and M. roseum, respectively. The heaviest elaiosome is M. roseum var. ovalifolium with 1.2mg, M. setaceum var. nakaianum has 1.0mg and the lightest elaiosome is M. roseum with 0.8mg. Major ant species frequently transporting the diaspores of Melampyrum species are Myrmicinae four species; Myrmica kotokui, M. excelsa, Myrmecina nipponica and Aphaenogaster japonica. Further studies on Melampyrum species, hemiparasite annual myrmecochorous plant, and related ant species should be needed.

Development and Applications of Pore-filled Ion-exchange Membranes (세공충진 이온교환막의 개발 및 응용)

  • Kim, Do-Hyeong;Kang, Moon-Sung
    • Membrane Journal
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    • v.28 no.5
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    • pp.307-319
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    • 2018
  • Ion-exchange membrane (IEM) has fixed charge groups and is a separation membrane which is capable of selectively transporting ions of the opposite polarity. Recently, the interest in IEMs has been increasing as the importance of the desalination and energy conversion processes using them as the key components has increased. Since the IEMs determine the efficiency of the above process, it is necessary to improve the separation performance and durability of them and also to lower the expensive membrane price, which is a hindrance to the widening application of the IEM process. Therefore, it is urgent to develop high-performance and low-cost IEMs. Among various types of IEMs, pore-filled membranes prepared by filling ionomer into a porous polymer substrate are intermediate forms of homogeneous membranes and heterogeneous membranes. The production cost would be cheap like the case of heterogeneous membranes because of the use of inexpensive supports and the reduction of the amount used of raw materials, and at the same time, they exhibit excellent electrochemical characteristics close to homogeneous membranes. In this review, major research and development trends of pore-filled IEMs, which are attracting attention as high-performance and low-cost IEMs, have been summarized and reported according to the application fields.

A Study on the operation status and improvement of EMU (Empty Management Unit) (EMU(Empty Management Unit)의 운영현황 및 개선방안에 관한 연구)

  • Kim, Ki-Young;Nam, Tae-Hyun;Kong, Jeong-Min;Yeo, Gi-Tae
    • Journal of Digital Convergence
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    • v.15 no.12
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    • pp.273-282
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    • 2017
  • The purpose of this study is to investigate the changes in the recovery of equipment using full containers loaded with freight instead of direct container transportation according to the sales policy considering the cost of transporting the containers. The EMU operation case was analyzed. The policies implemented by Company A to provide EMU credits depended only on the relative accountability contribution margin per box (RA CMPB) for the profitability of backhaul full freight. Therefore, these policies resulted in limitations in sales based on profitable accountability (PA) CMPB, which is more profitable. Moreover, while some Asian regions have surplus equipment, many Asian destinations to which their freight is exported have equipment shortages. Thus, in some cases, service sales to the regions with surplus equipment generated greater company-wide profits when compared with the return of empty containers or sales to the regions with insufficient equipment. In other words, the conditions for providing EMU credits should be changed, and even the full freight between regions with surplus equipment should be provided with credit amounts in which potential factory and transport costs in a destination are pre-reflected.

International Airfares and Application of Competition Laws (국제항공운임과 국내 경쟁법규의 적용)

  • Shin, Dong-Chun
    • The Korean Journal of Air & Space Law and Policy
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    • v.26 no.1
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    • pp.93-125
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    • 2011
  • The International Civil Aviation Convention (Chicago Convention) has been a backbone of international air transport system whereby air transport between States should be based on bilateral agreements, and in particular, international airfares, which are set up through IATA(International Air Transport Association) rate-fixing machinery could be approved by the governments concerned. International airfares are fares for transporting passenger and freight and their conditions between two or more countries. However, since U.S. pursued th deregulation policy in 1978 whereby routes, capacity and fares could be freely determined by airlines, many States have been following so called open-skies agreements. In many cases, aeronautical and competent authorities have been reviewing whether airlines' commercial activities including air fares could possibly conflict with free competition rules envisaged in relevant laws and regulations. As competition among airlines gets intense, airlines often resort to cooperation with other airlines in the forms such as equity exchange, M&A, code-sharing, fares consultation and resource pooling, mainly with a view to effectively use resources available and to avoid monopoly situation resulting from excessive and destructive competition among players. Whereas bearing in mind that application of competition laws is important to secure consumers' interests by preventing airlines's malpractices such as bargaining exorbitant fares, it is also important to comprehensively consider as many factors as possible, from that unilateral measure by governments may bring about retaliatory measures by the governments affected, to that airlines' cooperative practices may rather increase consumers' benefits by lowering air fares.

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A Quantitative Risk Analysis of Related to Tower Crane Using the FMEA (타워크레인의 정량적 위험성 평가가법에 관한 연구(FMEA 기법 위주))

  • Shim, Kyu-Hyung;Rie, Dong-Ho
    • Journal of the Korean Society of Safety
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    • v.25 no.6
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    • pp.34-39
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    • 2010
  • The purpose of this study is to suggest objective evaluation model as a plan to utilize as opportunity in establishing judgment standard of mutual inspection criteria and to upgrade inspection ability by reviewing and analyzing level of danger and importance in advance based on inspection results of inspection institutions regarding tower cranes used in construction fields. Tower crane is a mechanical device transporting construction supplies and heavy materials to places over 20~150M high from the ground for the period ranging from a short time of 2~3 months to two years after being installed in construction sites in vicinity of buildings or structures and is an important facility indispensable for construction sites. However, since use period after installation is short and professional technical ability of technicians working on-site about of tower crane is poor, systematic and quantitative safety management is not carried out As a part of researches on procedure of RBI(Risk Based Inspection) possible to apply to Knowledge Based System based on knowledge and experiences of experts as well as to tower cranes for solving these problems, quantitative RPN(Risk Priority Number) was applied to RPN utilizing technique of FMEA(Failure Mode and Effect Analyses). When general RBI 80/20 Rule was applied parts with high level of risks were found out as wire rope, hoist up/down safety device, reduction gear, and etc. However, since there are still many insufficient parts as risk analyses of tower crane were not established, it is necessary for experts with sufficient experiences and knowledge to supplement active RBI techniques and continuous researches on tower cranes by sharing and setting up data base of important information with this study as a starting point.

A Study on the Optimum Generation Condition of Ultrasonic Guided Waves for Insulation Pipelines (단열된 배관의 유도초음파 최적 발생조건 선정에 관한 연구)

  • Lee, Dong-Hoon;Cho, Hyun-Joon;Kang, To;Park, Dong-Jun;Kim, Byung-Duk;Huh, Yun-Sil;Lee, Yeon-Jae
    • Journal of the Korean Institute of Gas
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    • v.20 no.6
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    • pp.50-57
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    • 2016
  • Pipeline is one of the most abundant components in petrochemical plant. It plays a critical role in transporting fluids. Some pipelines are thermally insulated by wrapping them with insulating materials to prevent the loss of energy. However, when corrosion begins under insulation, it cannot be easily seen without unwrapping the cover, and thus corrossion should be detected using a non-destructive ways such as ultrasound guided wave. In this paper, the piping where the CUI (Corrosion Under Insulation) which occurs in the insulation parts guided waves effectively the optimum condition which is theoretical for selected guided waves phase velocity dispersion curve and wave-structure. The results of this study are expected to be directly utilized for onsite inspection of pipeline's CUI in many petrochemical plants.

Seasonal Variation of Residual Flow and Prospect for Sediment Transport in the Macrotidal Coastal Area (대조차 연안해역의 계절적 잔차류 변화와 퇴적물 이동 예상)

  • Lee, Jong Dae;Yoon, Byung Il;Kim, Jong Wook;Kim, Myung-Seok;Jeong, Jae-Soon;Woo, Seung-Buhm
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.32 no.1
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    • pp.39-47
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    • 2020
  • In order to investigate current characteristics at the Gungpyung beach, which is a macrotidal flat, field measurements were carried out in the summer and winter at the intertidal and the subtidal zones. The distribution of residual current at intertidal flat was dominant in the northward direction in the summer and dominant in the northward and southwestern directions in the winter. The direction and speed of the residual current in the winter are highly correlated with the significant wave height, and the turbidity is also highly correlated with the significant wave height. Therefore, in the winter, high sediment rates are suspended by high waves, and sediments are transporting due to the residual current in the southwest direction. On the other hand, it is expected that the northward residual current is predominant due to the small wave in the summer, and sediment transport does not occur largely due to less suspended sediments. In addition, sediment transport in the southern direction is blocked by the dock, which is the artificial structure, and the erosion occurs in the south side of the dock. The erosion pattern in the macrotidal zone of Yellow sea is dominated by seasonal waves, and blocking of sediments by artificial structure is very important.