• Title/Summary/Keyword: Transport functions

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Production of Leptin in E. coli and Its Effect on Glucose and Acetate Transport and Expression of Uncoupling Protein-2 Gene in Adipose Tissues of Korean Cattle (Hanwoo)

  • Kim, K.S.;Baik, M.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.8
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    • pp.1062-1068
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    • 2004
  • Leptin has a major role in the regulation of food intake and energy homeostasis. In addition, leptin participates in many physiological functions including regulation of lipid metabolism. Bovine recombinant leptin protein was produced in E. coli cells in order to understand function of leptin in the regulation of lipid metabolism. The leptin expression vector was constructed in pGEX-4T-3 vector and transformed into E. coli BL21 cells. Expression of the GST-leptin fusion protein was induced with IPTG. The fusion protein was purified using glutathione sepharose 4B batch method, and the recombinant leptin was eluted after thrombin protease digestion. The effect of leptin on glucose transport was examined in the differentiated adipocytes of 3T3-L1 cells. Leptin had no effect on basal and insulin-stimulated glucose transport in 3T3-L1 cells (p>0.05). Effect of recombinant leptin on glucose and acetate transport was examined in adipose tissues of Korean cattle (Hanwoo). Insulin stimulated glucose transport in both intramuscular and subcutaneous adipose tissues (p<0.05), but leptin did not affect glucose transport in both adipose tissues (p>0.05). Insulin stimulated acetate transport in bovine adipose tissues (p<0.05), but leptin did not affect acetate transport (p>0.05). Northern and RT-PCR analyses showed that mRNA levels of uncoupling protein-2 were increased by leptin treatment in 3T3-L1 cells without statistical difference (p>0.05). In conclusion, bovine recombinant leptin did not affect glucose and acetate transport in both 3T3-L1 adipocytes and bovine adipose tissues, while it stimulates UCP-2 mRNA expression in 3T3-L1 cells.

Perfecting the System for Assessment of the Financial Potential of a Transport Enterprise

  • Nesterov, Evgeny Aleksandrovich;Borisov, Andrei Viktorovich;Shadskaja, Irina Gennadievna;Shelygov, Aleksandr Vladimirovich;Sharonin, Pavel Nikolaevich;Frolov, Alexander Lvovich;Lebedeva, Olga Yevgenievna
    • International Journal of Computer Science & Network Security
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    • v.22 no.7
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    • pp.109-116
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    • 2022
  • The article is devoted to perfecting the system of management of the financial potential of transport enterprises. It is established that transport as an integral part of the state economy has to organically enter the market economy and provide sustainable transport services to national economy enterprises regardless of ownership, as well as ensure passenger transportation. It is also determined that in the conditions of market relations, transport highways must perform their functions with sufficient economic benefit to keep their material and technical resources in good order, conduct an investment policy with extensive use of scientific and technological progress, as well as a social policy guaranteeing the conditions for employees' motivated work. The study reveals an association between the financial and strategic goals of transport enterprises and the minimization of their economic risks, the prevention of bankruptcy and profit margin shortfalls. It is found that transport enterprises need to strive for the overall improvement of their financial potential through increasing the components of financial potential and assessing the impact of risk factors on them: the capacity of fixed assets, the capacity of financial resources, the capacity of services, and the capacity of credit opportunities. These are the elements of transport enterprises' financial potential that ensure its desired level. It is demonstrated that of critical importance in managing the financial potential of a transport enterprise is the role of financial resources, as a subject cannot reach the desired strategic goals without them.

VLSI Design and Implementation of Multimedia Transport Protocol for Reliable Networks

  • Jong-Wook Jang
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.1 no.1
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    • pp.21-33
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    • 1997
  • This dissertation deals with the design and VLSI implementation of the MTP(Multimedia Transport Protocol) protocol for the high speed networks. High throughput, functional diversity and flexible adaptation are key requirements for the future transport protocol. However it is very difficult to satisfy all these requirements simultaneously. Fortunately, the future networks will be very reliable. It means that the future transport protocol will usually perform some fixed functions without the protocol state information. According to this concept, we proposed and designed the MTP protocol that is consisted of Information Plane and Control Plane. Information Plane performs some fixed functions that are independent of the protocol state information as far as no error. However Control Plane manages the protocol state information and controls the operation of Information Plane. Our MTP protocol was finally implemented as an FPGA chip using the VHDL. We built a testbed for verification of the implemented protocol, and it was shown that the MTP protocol worked correctly and made a throughput of about 800 Mbps. Our future works include the addition of multiplexing and multicasting capabilities to our protocol for multimedia applications.

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VALIDATION OF A DESIGN CODE FOR SODIUM-TO-SODIUM HEAT EXCHANGERS BY UTILIZING COMPUTATIONAL FLUID DYNAMICS (전산유체역학을 이용한 소듐-소듐 열교환기 설계코드의 검증)

  • Kim, D.;Eoh, J.H.;Lee, T.H.
    • Journal of computational fluids engineering
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    • v.21 no.1
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    • pp.19-29
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    • 2016
  • A Prototype Gen-IV Sodium-cooled Fast Reactor which is one of the $4^{th}$ generation nuclear reactors is in development by Korea Atomic Energy Research Institute. The reactor is composed of four main fluid systems which are categorized by its functions, i.e., Primary Heat Transport System, Intermediate Heat Transport System, Decay Heat Removal System and Sodium-Water Reaction Pressure Relief System. The coolant of the reactor is liquid sodium and sodium-to-sodium heat exchangers are installed at the interfaces between two fluid systems, Intermediate Heat Exchangers between the Primary Heat Transport System and the Intermediate Heat Transport System and Decay Heat Exchangers between the Primary Heat Transport System and the Decay Heat Removal System. For the design and performance analysis of the Intermediate Heat Exchanger and the Decay Heat Exchanger, a computer code was written during previous step of research. In this work, the computer code named "SHXSA" has been validated preliminarily by computational fluid dynamics simulations.

An Experimental Investigation on the Operating Characteristics of a Reversible Loop Heat Pipe (가역 루프 히트파이프의 작동특성에 관한 실험적 연구)

  • Kim Bong-Hun;Choi Joon-Min
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.18 no.3
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    • pp.231-239
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    • 2006
  • An experimental investigation of a Reversible Loop Heat Pipe (RLHP) was conducted to determine the operating limits and performance characteristics as functions of the thermophysical parameters, the heat input, and the cooling intensity. Variations in both temperature and heat transport capacity were measured and analyzed in order to accurately evaluate the transient operating characteristics. In addition, the maximum heat transport as a function of the mean evaporator temperature, the ratio of heat transport to heater input power as a function of the mean evaporator temperature, and the overall thermal resistance as a function of the overall heat transport capacity were examined as well. Results indicated that the cooling intensity played an important role on the operating characteristics and performance limitation. The maximum heat transports corresponding to cooling intensity $72W/^{\circ}C$ and $290W/^{\circ}C$ were 446 W and 924 W, respectively. Also, observation of the startup characteristics indicated that the mean evaporator temperature should be maintained between $40^{\circ}C$ and $60^{\circ}C$, and overall thermal resistance were measured as $0.02^{\circ}C/W$.

Study on the Electron Transport Coefficient in Mixtures of $CF_4$ and Ar ($CF_4-Ar$ 혼합기체의 전자수송계수에 관한 연구)

  • Kim, Sang-Nam
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.56 no.1
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    • pp.1-5
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    • 2007
  • Study on the electron transport coefficient in mixtures of CF4 and Ar, have been analyzed over a range of the reduced electric field strength between 0.1 and 350[Td] by the two-term approximation of the Boltzmann equation (BEq.) method and the Monte Carlo simulation (MCS). The calculations of electron swarm parameters require the knowledge of several collision cross-sections of electron beam. Thus, published momentum transfer, ionization, vibration, attachment, electronic excitation, and dissociation cross-sections of electrons for $CF_4$ and Ar, were used. The differences of the transport coefficients of electrons in $CF_4$ mixtures of Ar, have been explained by the deduced energy distribution functions for electrons and the complete collision cross-sections for electrons. The results of the Boltzmann equation and the Monte Carlo simulation have been compared with the data presented by several workers. The deduced transport coefficients for electrons agree reasonably well with the experimental and simulation data obtained by Nakamura and Hayashi. The energy distribution function of electrons in $CF_4-Ar$ mixtures shows the Maxwellian distribution for energy. That is, $f({\varepsilon})$ has the symmetrical shape whose axis of symmetry is a most probably energy. The proposed theoretical simulation techniques in this work will be useful to predict the fundamental process of charged particles and the breakdown properties of gas mixtures. A two-term approximation of the Boltzmann equation analysis and Monte Carlo simulation have been used to study electron transport coefficients.

Application of Landscape Ecology to Watershed Management : How can We Restore Ecological Functions in Fragmented landscape\ulcorner (유역관리에서 경관생태학의 응용 : 절개된 경관의 생태적 기능을 어떻게 회복시킬 수 있을까\ulcorner)

  • Nakamura, Futoshi
    • The Korean Journal of Ecology
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    • v.21 no.4
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    • pp.373-382
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    • 1998
  • This paper describes the ecological structure and function of riparian zone, and their historical changes with land-use. The riparian zone consists of valley floor landform and riparian vegetation. The functions discussed are attenuation of sunlight energy, input of leaves and needles, contribution of woody debris to streams, and retention of flowing material out of transport. These primary functions directly or indirectly influence water and sediment qualities of streams, bars and floodplains, and thereby aquatic biota. Temporal changes in a hydrological system and riparian ecosystem were examined with reference to land-use conversin in order to understand the linkages between these two systems in Toikanbetsu River. The influences of channelization and land-use on discharge of suspended sediment and wetland vegetation was also investigated in Kushiro Marsh. These two examples suggested that the ecological functions of riparian zone have been degraded as flood control and reclamation works have expanded in the past twenty years The author proposes river restoration planning by preserving or creating landscape elements based on the concepts of sustaining physical and ecological linkages.

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Realization of a Scalable and Reliable Multicast Transport Protocol for Many-to-Many Sessions

  • Lee, Seung-Ik;Ko, Yang-Woo;Lee, Dong-Man
    • ETRI Journal
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    • v.29 no.6
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    • pp.745-754
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    • 2007
  • In this paper, we present the Enhanced Communication Transport Protocol-Part 5 (ECTP-5), which provides scalable and reliable multicast communication service for many-to-many applications by constructing high quality recovery trees from two-layer logical trees and repairing the losses via unicast automatic repeat request-based error control. In order to realize the protocol, we developed feasible protocol architectures and building blocks including additional functions which deal with engineering details, such as membership dynamics and sender coordination. Experimental results show that ECTP-5 scales well with various session sizes and packet loss rates in terms of control overhead and recovery latency.

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Design and Performance Evaluation of OBP Satellite B-ISDN Transport Network Architecture (OBP 탑재 n이성 B-ISDN 중계망 구조 설계 및 성능 평가)

  • Park, Seok-Cheon
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.3
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    • pp.901-908
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    • 2000
  • Satellite communication in the 21 century's high tech information world is developing rapidly, marked by high levels of applications and functions. For example, satellite communication can process and switch the speed of the service provided by a broad and vast digital multimedia system such as a long-distance all between nations or broadcasting transfer service, which is supplied by a contemporary satellite system. So, it bring about problems which lack of satellite orbit and gibes out frequency resource by increment of satellite universally. To support this, an OBP satellite system is need, which includes an on-board IF/RF switch, baseband signal processing, multi-beam antenna technology, as well as a simple transponder system. In this paper, we have outlined the next generation of satellite communication; satellite OBP transport network architecture, which offers multimedia service and applied frequency reuse method for multi-spot beam. The satellite B-ISDN transport network architecture is also analyzed.

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