• 약학회지
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• 제47권2호
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• pp.110-119
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• 2003

The purpose of the present study was to kinetically investigate the carrier-mediated uptake in the hepatic transport of organic anions, and to simulate the ″in vivo counter-transport″ phenomena, using kinetic model which was developed in this study. The condition that the mobility of carrier-ligand complex is greater than that of free carrier is not essential for the occurrence of ″counter-transport″ phenomenon. To examine the inhibitory effects on the initial uptake of a ligand by the liver, it is necessary to judge whether the true counter-transport mechanism (trans-stimulation) is working or not. The initial plasma disappearance curves of a organic anion were then kinetically analyzed based on a flow model, in which the ligand is eliminated only from the peripheral compartment (liver compartment). Moreover, ″in vive counter-transport″ phenomena were simulated based on the perfusion model which incorporated the carrier-mediated transport and the saturable intracellular binding. The ″in vivo counter-transport″ phenomena in the hepatic transport of a organic anion were well demonstrated by incorporating the carrier-mediated process. However, the ″in vivo counter-transport″ phenomena may be also explained by the enhancement of back diffusion due to the displacement of intracellular binding. In conclusion, one should be more cautious in interpreting data obtained from so-called ″in vivo counter-transport″ experiments.

• 대한의생명과학회지
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• 제12권1호
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• pp.17-22
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• 2006

The baculovirus expression system is a powerful method for producing large amounts of the human erythrocyte-type glucose transport protein, heterologously. Characterization of the expressed protein is expected to show its ability to transport sugars directly. To achieve this, it is a prerequisite to know the properties of the endogenous sugar transport system in Spodoptera frugiperda Clone 21 (Sf21) cells, which are commonly employed as a host permissive cell line to support the baculovirus replication. The Sf21 cells can grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transport system. However, unlike the human glucose transport protein that has a broad substrate and inhibitor specificity, very little is known about the nature of the endogenous sugar transport system in Sf21 cells. In order to characterize further the inhibitor recognition properties of the Sf21 cell transporter, the ability of phloretin, cytochalasin B and D-fructose to inhibit 2-deoxy-D-glucose (2dGlc) transport was examined by measuring inhibition constants $(K_i)$. The $K_i's$ for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. The 2dGlc transport in the Sf21 cells was very potently inhibited by phloretin, the aglucone of phlorizin with a $K_i$ similar to the value of about $2{\mu}M$ reported for inhibition of glucose transport in human erythrocytes. However, the Sf21 cell transport system was found to differ from the human transport protein in being much less sensitive to inhibition by cytochalasin B (apparent $K_i$ approximately $10\;{\mu}M$). In contrast, It is reported that the inhibitor binds the human erythrocyte counterpart with a $K_d$ of approximately $0.12\;{\mu}M$. Interestingly, the Sf21 glucose transport system also appeared to have high affinity for D-fructose with a $K_i$ of approximately 5mM, contrasting the reported $K_m$ of the human erythrocyte transport protein for the ketose of 1.5M.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.442-451
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• 2019

Objective: This study was conducted to investigate the effect of transport stress on physiological and hematological responses and milk performance in lactating dairy cows. Methods: Ten lactating dairy cows were randomly divided into 2 groups. The treatment group (TG) was transported 200 km for 4 h by truck, and the control group (NTG) was restrained by stanchion for 4 h in Konkuk University farm. Blood and milk samples were collected at 24 h pre-transport; 1, 2, and 4 h during transport; and 2, 24, and 48 h post-transport. Milk yields were measured at 24 h pre-transport, 0 h during transport, and 24, 48, and 72 h post-transport. Results: Leukocyte, neutrophil, and monocyte numbers in the TG were significantly higher than those of the NTG at each experimental time point. Lymphocyte numbers in the TG were significantly (p<0.05) higher than those of the NTG at 48 h post-transport. Additionally, the neutrophil:lymphocyte ratio of the TG was 45% and 46% higher than that of the NTG at 4 h during transport and 2 h post-transport, respectively. There were no significant differences in erythrocyte numbers, hemoglobin concentrations, platelet numbers, and hematocrit percentages between two groups. Cortisol levels in the TG were significantly (p<0.05) higher than those in the NTG. Milk yields in the TG were lower than those in the NTG. The somatic cell count (SCC) of the TG was significantly (p<0.05) higher than that of the NTG at 1 and 2 h during transport; that of the TG increased dramatically at 1 h during transport and gradually decreased subsequently. Conclusion: Transport stress increased blood parameters including leucocyte, neutrophil, and monocyte numbers by increased cortisol levels, but did not affect erythrocytes, hemoglobin and hematocrit levels. Additionally, transport resulted in a decrease in milk yield and reduced milk quality owing to an increase in milk SCC.

• 무역상무연구
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• 제69권
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• pp.303-326
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• 2016

The Rotterdam Rules regulate both transport documents and the legal effect of the choice of document much more comprehensively than the existing maritime convention to bring international harmonization of issues relating to transport documents. The Rotterdam Rules use the generic term 'transport documents' rather than referring to specific title such as bills of lading, sea waybills. The generic term 'transport documents' allow four types of transport documents to be identified as follows. 1. negotiable 2. negotiable which dispense with surrender 3. non-negotiable which require surrender 4. non-negotiable. Each types of transport documents has its requirements to be satisfied. Also, the choice of transport documents affects legal effect. Thus parties to the contract of carriage not only need to know how the document will be classified at the time it is issued but also consider what the documents will bring legal consequences.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.1668-1673
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• 2007

In light of the growing traffic congestion problem and congestion cost, the container transport by railway has to be increased. The freight transport by railway can have decided advantages over trucks in terms of energy efficiency, emissions and cost for certain freight movements, just as transportation in the metropolitan region can have great advantages over driving truck. But the freight transport by truck should gain significant mobility benefits from a freight railway system. Thus, the DMT(Dual Mode Trailer) transport system which is coupled railway transport advantages with load transport advantages has been developed and used in the european countries. The DMT transport will therefore serve the areas required by transport organizers. This paper describes the various DMT transport systems and its strength and weakness.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2015년도 추계학술대회
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• pp.241-242
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• 2015

According to globalization of world economy on distribution and sales, logistics and transportation parts are play important role. Especially, it important to know and study how to choose right transport route and which is the key factor of route choice model in multimodal transport system. Thus this study aims to consider 4 main factors: cost, delivery time, freight, and logistics service factors additionally dividing into 13 sub factors and object is forwarders between Mongolia and Korean freight transport. The survey is based on AHP through interview with company officials. The paper provides empirical insights about current status of Mongolian forwarders and difference of the important factors between transportation modes. Result shows that time factor is role factor to choose transport route and then cost factors. Additionally, this study shows 2 different route choose factors between air transport and shipping transport forwarders.

• 무역상무연구
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• 제46권
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• pp.181-210
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• 2010

The continuing advance of multimodal transport with the importance for efficient and effective logistics management emphasizes the need for uniform legal approach to international multimodal transport. However, the current fragmented instrument regulating such transport is being an obstacle to development of multimodal transport as it aggravates confusion and uncertainty. The Rotterdam rules, which was adopted in December 2008 by UNCITRAL, expands its scope of application to door-to-door transport. However, the new rules has some problems in its application to multimodal transport operation as it has been conceived not to regulate general multimodal carriage but to regulate contract of carriage by sea that extends its services to the transport by other modes. This article examines conflict of conventions in the Rotterdam Rules. The applicability of the Rotterdam Rules in international multimodal transport contract and possibility of potential conflict with other transport conventions are analyzed with some hypothetical cases. Furthermore, problems arising from application of the Rotterdam Rules under international multimodal transport Contracts are indicated in the chapter IV.

• 정보처리학회논문지C
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• 제9C권5호
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• pp.627-636
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• 2002

이 논문에서는 이동통신망의 무선접속망(RAN . Radio Access Network)에서 ATM 전송 방식과 IP$_v$4 전송 방식 그리고 IP$_v$6전송 방식이 혼재하여 존재할 때, 다른 전송 방식들간의 연동을 지원하는 방법을 제시한다. 제시된 연동 방법은 하나의 노드안에 세 가지 전송 방식에 대한 각각의 기능모듈을 모두 구현하는 Triple Capable 노드 방법으로 다양한 전송 방식을 가진 노드들과 직접 연동할 수 있도록 설계되었다. 그리고 제시된 Triple Capable 노드 방법과 같이 하나의 노드가 다수의 전송 방식중에서 하나의 전송 방식을 선택하여 사용하는 것이 가능할 때, 네트워크 통신 상황에 따라서 통신 성능을 향상시킬 수 있도록 전송 방식을 동적으로 선택하여 사용하는 알고리즘을 제시한다.

• 한국환경과학회지
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• 제13권3호
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• pp.263-277
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• 2004

The purpose of this study is to analyze the characteristics of riverbed variation due to the sediment protection weir located on the estuary of the main stream of Taehwa river using I-D finite difference model, HEC-6 model, and the followings are the results of estimating sediment transport rate, amount of scour or deposition, and accumulated amount of deposit according to before and after of the sediment protection weir removal with various flow rates in the channel. Ackers-White transport function produced the greatest sediment transport rate while Meyer-Peter showed the smallest sediment transport rate at the most down stream area of the watershed through the sediment transport rate analyses for various flow rates according to the existence or nonexistence of the sediment protection weir. Toffaleti's and Colby transport function were closest to the average value, and the difference among the results of the sediment transport functions showed up to 8～9 times. Duboy's transport function produced the greatest riverbed variation while Toffaleti's showed the smallest variation through the riverbed variation analyses according to the existence or nonexistence of the sediment protection weir. Yang's was closest to the average value, and the difference among the results of the riverbed variation analyses ranged from 1.4 times to 11 times. It is thought that a sediment transport function must be selected very carefully with respect to the criteria of sediment yield estimation because the analysis results of the sediment transport rate and riverbed variation according to flow rates showed significant differences among the sediment transport functions, and the differences of sediment transport rate and riverbed variation according to the various sediment transport functions decreased as the flow rate increased.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2003년도 춘계공동학술대회논문집
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• pp.304-310
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• 2003

본 논문에서는 운송시간과 운송비용으로 구성된 희생량 모델과 운송경로의 환경비용을 이용한 수출입 컨테이너화물의 운송경로 선택에 관하여 고찰한다. 먼저 희생량 모델, 운송의 환경비용, 수출입 컨테이너화물의 물동량과 운송경로별 분담율에 대하여 간단히 살펴본다. 그리고 부산항의 수출입 컨테이너화물을 서울로 운송하는 경우를 가정하고 운송시간과 운송비용, 화물의 운송에 의하여 발생하는 환경비용에 대하여 고찰한다. 또한 환경비용을 고려한 희생량 모델을 이용하여 각 운송경로별 희생량을 계산한다. 마지막으로 계산된 운송경로의 희생량에 기초하여 수출입컨테이너 화물의 운송경로를 결정한다. 본 연구의 결과, 운송의 환경비용을 고려하는 경우 수출입컨테이너의 육상운송 분담율이 감소하며 연안운송 분담율이 증가하는 것을 확인하였다.