• Title/Summary/Keyword: Transmission

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Exosomes isolation from bovine serum: qualitative and quantitative comparison between ultracentrifugation, combination ultracentrifugation and size exclusion chromatography, and exoEasy methods

  • Eun-Yeong Bok;Sang Young Seo;Han Gyu Lee;Sudu Hakuruge Madusha Pramud Wimalasena;Eunju Kim;Ara Cho;Young-Hun Jung;Tai-Young Hur;Kyoung-Min So;Sung-Lim Lee;Yoon Jung Do
    • Journal of Animal Science and Technology
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    • v.66 no.5
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    • pp.1021-1033
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    • 2024
  • Exosomes have been extensively studied as disease biomarker in humans, given their role in transporting bioactive molecules. However, despite the great potential of exosomes as noninvasive diagnostic markers and therapeutic nanocarriers for bovine diseases, few studies have been conducted on bovine exosome. Thus, this study aimed to quantitatively and qualitatively compare three isolation methods to identify a suitable method for bovine serum. Exosomes were isolated using ultracentrifugation alone (UC), a combination of ultracentrifugation and size exclusion chromatography (US), or membrane affinity-based exoEasy kit (EE). Isolated particles were evaluated using a range of complementary techniques. Transmission electron microscopy showed that all three isolation methods resulted in particles with a cup-shaped morphology. The particle concentration measured by nanoparticle trafficking analyzer of US was lower compared to those of UC and EE method. As a result of immunoblotting, exosome markers including TSG101, CD81, and HSP70 were detected in US particles, while in UC and EE, only TSG101 expression was confirmed. Particles isolated from UC and EE showed a contamination with the blood protein albumin, whereas particles from US did not show albumin contamination. In addition, to evaluate the possibility of using exosomes as biomarkers, the profiles of the small RNA in the exosomes were compared using the bioanalyzer 2100. As a result, in the EE method, the band of small RNA (25-200 nt) was most prominent, and in the US methods, a distinct band was observed in the small RNA range. Collectively, the purity of exosomes without non-exosomal contamination was highest in the US method. However, for the detection of small RNA, the EE method was found to be the most suitable. Therefore, the results suggest that the optimal isolation method varies depending on the specific purpose of exosome isolation.

Noble Women's Culture and Perception of Incense and Herbs in the Late Joseon Dynasty based on 『Gyuhap Chongseo』, 「BongimCheok」 (『규합총서』 「봉임측」에 나타난 조선후기 사대부 여성의 향문화(香文化)와 인식)

  • HA Sumin
    • Korean Journal of Heritage: History & Science
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    • v.57 no.3
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    • pp.38-54
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    • 2024
  • This paper examines how noblewomen during the Joseon Dynasty perceived incense and herbs, focusing on the 『Gyuhap Chongseo(閨閤叢書)』 a compendium edited by Yi Bingheogak(李憑虛閣, 1759~1824). The 『Gyuhap Chongseo』 is a classified collection of knowledge deemed essential for managing a noble household. By analyzing the content related to incense and herbs focused on 「Bongim Cheok」. This study reveals Yi Bingheogak's practical and realistic understanding of incense and herbs. For Yi, incense and herbs transcended mere cosmetic applications and luxury and served as indispensable tools in health and hygiene and domestic skills for traditional household management. While it may be challenging to generalize the perception of noblewomen solely based on the 『Gyuhap Chongseo』 the consistent transcription and transmission of the text suggest it resonated with the noblewomen's sentiments. With limited records left by noblewomen themselves, this study primarily explores Yi Bingheogak's perspective, who provided detailed knowledge and applications of incense and herbs across the compendium's sections. This reflects Yi's intention to broaden women's knowledge and cultural literacy, and contribute to improved quality of life through practical knowledge. This study demonstrates Yi's recognition of incense and herbs as vital commodities for the operation of domestic life, and illustrates how noblewomen of the Joseon Dynasty actively contributed to society and the household through knowledge and practical activities. The use of incense and herbs for beauty and domestic knowledge in various fields of traditional knowledge underscores a comprehensive understanding of noblewomen's perception and use of incense and herbs during the Joseon Dynasty.

The Effect of Breathing Meditation Qigong Therapy on the Recovery of Olfactory Disorders and Voice Handicap Index in Parkinson's Disease Patients (호흡명상기공테라피가 파킨슨병 환자의 후각 및 음성 기능장애 개선에 미치는 효과)

  • So Jung An;Hun Mo Ahn
    • Journal of Korean Medical Ki-Gong Academy
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    • v.23 no.1
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    • pp.10-29
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    • 2024
  • Objective : The purpose of this study is to determine whether An's breathing meditation qigong therapy (ABMQT) delivers bioenergy to the frontal lobe, prefrontal lobe, the olfactory tract in the mesolimbic pathway, olfactory bulb, CV22, CV21, olfactory area and vocal-related areas in Parkinson's disease (PD) patients to help improve olfactory disorders (anosmia) and vocal functions. Methods : The subjects of this study were 4 patients with idiopathic PD (3 males/1 female, 65.0±NA/68.7±10.2 years old). ABMQT was applied once a week, 120 minutes per session for 12 weeks in a non-invasive and noncontact manner, and the test before and after ABMQT application included olfactory impairment test the Korean version of Sniffin' stick test (KVSS), voice acoustic test, aerodynamic test, vocal handicap index (VHI-30), and auditory perception scale test tools. The results before and after the experiment were analyzed assuming a normal distribution, and a chi-square test was performed using a continuity correction, and the significance level was set to p<0.05. And the medical diagnosis and findings of the examiner (doctor in charge) before and after the experiment were described. Results : KVSS was significant as 0.2±0.5 and 9.0±0.0 before and after the experiment. There was no significant difference between the voice acoustic test FO and Jitter, the vocal aerodynamic test MPT, SP, AE, the vocal disorder index test, and the auditory perception test. However, the medical diagnosis findings of four study subjects showed that olfactory disorders, voice disorders, and laryngeal function were improved before and after the application of ABMQT. Conclusions : The breathing meditation qigong program showed significant effects on improving the olfactory disorders (anosmia) and speech function of each study subject. However, to produce meaningful results, it is thought that experiments involving a larger number of research participants are necessary, and additional blood and FMRI tests are conducted to verify metabolic activities and the olfactory neuron signal transmission system.

Research on the Development and Application of Home Economics Education Class Modules for Convergence Education (융복합 교육을 위한 가정과교육 수업모듈 개발 및 적용 연구)

  • Park, Ji Soon;Ju, Sueun
    • Journal of Korean Home Economics Education Association
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    • v.35 no.3
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    • pp.135-149
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    • 2023
  • The purpose of this study is to develop and implement an integrated course model that centers around the subject of Home Economics Education Curriculum and Teaching Methods and its pedagogical approaches, as well as the subject of Chinese Language and Literature Curriculum and Teaching Methods and its pedagogical methods. This study aims to provide a framework to prepare pre-service teachers to effectively address a variety of educational issues in future educational settings. To achieve these objectives, the study utilizes Fogarty's connected model as a guiding framework to explore the impact of the integrated curriculum on fostering collaborative and divergent thinking among students. The findings of this research confirm that this model not only cultivates interdisciplinary competencies among course participants but also goes beyond the mere transmission of knowledge to build the capacities needed for forming an educational community, thereby increasing course satisfaction. Additionally, the study substantiates the importance of learner-centered strategies, cooperative learning, and diverse evaluation mechanisms. Such an integrated course model has the potential to revolutionize not only pre-service teacher education but also to be applicable in in-service teacher training, thus contributing to solving a broader range of educational issues.

Microstructure and Abnormal Grain Coarsening Behavior of Nb-microalloyed Steel (Nb 첨가 합금강의 미세조직과 결정립 조대화 거동)

  • Sungjin Kim;Jeonghu Choi;Minhee Kim;Minhwan Ryu;Jaehyun Park;Jaehyeok Sin;Woochul Shin;Minwook Kim;Jae-Gil Jung;Seok-Jae Lee
    • Journal of the Korean Society for Heat Treatment
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    • v.37 no.4
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    • pp.155-162
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    • 2024
  • SCr420H steel which is commonly utilized for automotive components requires the carburizing heat treatment process. Abnormal grain growth during this treatment significantly affects the mechanical properties of the steel parts. Consequently, a process designed to prevent abnormal grain growth at certain elevated temperatures is essential. For enhanced grain refinement, we considered the addition of Nb in SCr420H steel. The experimental condition of the carburizing heat treatment involved reheating the steel sample to temperatures between 940℃ and 1080℃. Using scanning electron microscopy, we examined the microstructure of specimens treated with the secondary solution, revealing an organization of bainite and ferrite. Transmission electron microscopy was utilized to determine the type, shape, and size of the carbonitrides, showing a high fraction of AlN at the secondary solution treatment temperature of approximately 1050℃ and of (Nb,Ti)(C,N) around 1200℃. AlN particles measured about 100 nm and (Nb,Ti)(C,N) about 50 nm. Optical microscopy was utilized to assess grain size variations at different secondary solution treatment temperatures. It is noted that the temperature at which abnormal grain coarsening occurred rose with increasing secondary solution treatment temperatures, indicating a greater influence of (Nb,Ti)(C,N) with higher heat treatment temperatures. This research provides reference data for preventing abnormal grain growth in Nb-added low alloy steels undergoing carburizing heat treatment.

Effect of Ti Addition on the Microstructure and Grain Coarsening of SCR420H Steel (SCR420H강의 미세조직과 결정립 조대화에 미치는 Ti 첨가 영향)

  • Jeonghu Choi;Sungjin Kim;Minhee Kim;Jaehyun Park;Jaehyeok Sin;Minhwan Ryu;Woochul Shin;Minwook Kim;Seok-Jae Lee;Jae-Gil Jung
    • Journal of the Korean Society for Heat Treatment
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    • v.37 no.4
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    • pp.163-171
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    • 2024
  • SCR420H steel is a low-carbon chromium alloy steel designed for carburizing heat treatment. Recently, research is being conducted on high-temperature carburization heat treatment to reduce costs and CO2 emissions by shortening the carburization time to meet the international carbon neutral policy. However, this high-temperature carburization heat treatment coarsens the steel grains and causes a decrease in mechanical properties. In this study, a large amount of Ti was added to increase the grain refinement effect in the high-temperature carburizing process. We investigated the microstructure and precipitates of SCR420H steel without Ti (Al steel) and with Ti (AlTi steel). Thermodynamic calculations showed that the AlN and (Ti,Nb)(C,N) precipitated in Al steel, while (Ti,Nb)(C,N) and Ti4C2S2 precipitated in AlTi steel. Addition of Ti increases the fraction of bainite after reheating process. Transmission electron microscopy analysis shows that small amounts of AlN and (Ti,Nb)(C,N) precipitates are formed in the Al steel. The addition of Ti increases the density of (Ti,Nb)(C,N) precipitates and induces the formation of Ti4C2S2 precipitates, increasing the grain coarsening temperature (GCT) under all heat treatment conditions. Higher reheating temperatures also resulted in higher GCT values due to increased precipitation.

Research on Optimization Strategies for Random Forest Algorithms in Federated Learning Environments (연합 학습 환경에서의 랜덤 포레스트 알고리즘 최적화 전략 연구)

  • InSeo Song;KangYoon Lee
    • The Journal of Bigdata
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    • v.9 no.1
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    • pp.101-113
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    • 2024
  • Federated learning has garnered attention as an efficient method for training machine learning models in a distributed environment while maintaining data privacy and security. This study proposes a novel FedRFBagging algorithm to optimize the performance of random forest models in such federated learning environments. By dynamically adjusting the trees of local random forest models based on client-specific data characteristics, the proposed approach reduces communication costs and achieves high prediction accuracy even in environments with numerous clients. This method adapts to various data conditions, significantly enhancing model stability and training speed. While random forest models consist of multiple decision trees, transmitting all trees to the server in a federated learning environment results in exponentially increasing communication overhead, making their use impractical. Additionally, differences in data distribution among clients can lead to quality imbalances in the trees. To address this, the FedRFBagging algorithm selects only the highest-performing trees from each client for transmission to the server, which then reselects trees based on impurity values to construct the optimal global model. This reduces communication overhead and maintains high prediction performance across diverse data distributions. Although the global model reflects data from various clients, the data characteristics of each client may differ. To compensate for this, clients further train additional trees on the global model to perform local optimizations tailored to their data. This improves the overall model's prediction accuracy and adapts to changing data distributions. Our study demonstrates that the FedRFBagging algorithm effectively addresses the communication cost and performance issues associated with random forest models in federated learning environments, suggesting its applicability in such settings.

The Dragon Symbol and its Meaning in the Painting Poetry (제화시에 나타난 용(龍)의 표상과 그 의미)

  • Park Hae-young
    • Journal of the Daesoon Academy of Sciences
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    • v.49
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    • pp.157-186
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    • 2024
  • This study aims to understand the symbolic meanings of dragons in the contemporary period through the painting featuring poetry based on the Hwaryong (paintings of dragons) and to analyze the literary representations and characteristics of dragons. First, the shape and aura of the dragon in the paintings should be confirmed. In dragon Painting Poetry (Paintings Featuring Poetry), the shape of the dragon is embodied in the aura of 'spirit.' However, people in previous eras customarily believed that dragons were real. In addition, the main spirit of the Hwaryong was in the representation of the dragon's aura, or "Jeonsin (transmission of spirit)," such as the creature's dragonly virtue or Heavenly Perfection. Next, the rhetorical representation of the dragon in the painting is to be examined. In the actual Painting Poetry, dragon images were relatively common in descriptions of pines, bamboo, and plum trees. The pine-bamboo-plum motif is known as Sehan Samwu (the three friends of the cold season), and since the Northern Song Dynasty, it has become a symbol of literary incision. Manifested as a dragon, the pine-bamboo-plum motif has a common denominator of winding branches, tangled stems, and bluish jade coloration, and like the dragon, it is used as a backdrop for weather phenomena such as rain, wind, and lightning. In the schematic that connects the Hwaryong, dragon Painting Poetry, the original meaning of the dragon is transformed into shapes and language. This is the meaning conveyed by the dragon, and this symbolic meaning was shared among the writers of the period who viewed the poems and paintings.

A Study on the Establishment and Utilization of e-SOP System based on One-site Action Manual of Local Government (현장조치 행동매뉴얼 기반의 e-SOP 시스템 구축 및 활용 방안 연구)

  • Duckgil Kim;Yuri Kim;Daewon Jang;Yonsoo Kim
    • Journal of Wetlands Research
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    • v.26 no.3
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    • pp.266-271
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    • 2024
  • In order to minimize damage from disasters, rapid initial measures using disaster response manuals are needed. However, the existing disaster response manual is written in the form of a vast booklet, so there is a limit to accurately understanding the contents of the manual and using it for disaster response. Therefore, this study established an e-SOP system based on the disaster response manual, and suggested countermeasures using the e-SOP in the event of a disaster. Based on the guidelines for preparing the on-site action manual, the main functions of the e-SOP consisted of situation propagation, situation reporting, and situation management. In order to increase the on-site responsiveness and usability of the system, the bottom-up reporting system was strengthened so that the person in charge could report the response situation of the site to the situation room using the situation reporting function. In addition, the situation room was able to identify and manage the progress of disaster response work and support decision-making through situation reports delivered from the field.

Study on Vibration Reduction Rates of Barrier Walls under Load Transmission of High-Speed Trains at 180 km/h (시속 180 km 고속열차 하중전달시 차단벽의 진동저감율 연구)

  • Young-Min Kim;Sung-Wook Choi;Kang-Il Lee
    • Journal of the Society of Disaster Information
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    • v.20 no.3
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    • pp.549-562
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    • 2024
  • Purpose: In this study, numerical analysis was conducted to verify the vibration reduction effect of installing vibration barriers under various installation conditions to mitigate train-induced vibrations from the GTX. Method: To identify the factors influencing vibration reduction among the installation conditions, the stiffness ratio of the filling material and the installation depth of the barrier were varied. Result: The study results indicated that using ductile filling materials provided superior vibration reduction compared to hard filling materials. The vibration reduction effect was found to be more significant when the stiffness ratio between the ground and the filling material was closer to zero. Additionally, the deeper the installation depth of the barrier, the better the vibration reduction effect. Conversely, if the barrier was installed too shallowly, vibration at the measurement point was amplified. Conclusion: The optimal installation condition for vibration reduction was found to be a stiffness ratio of 0.08 and an installation depth of 15 meters, resulting in a vibration reduction rate of 60.34% at a measurement point 10 meters away from the vibration source.