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Egg Development and Larvae and Juveniles Morphology of Carp, Cyprinus carpio in Korean

  • Park, Jae Min;Mun, Seong Jun;Yim, Hu Sun;Han, Kyeong Ho
    • 한국발생생물학회지:발생과생식
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    • 제21권3호
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    • pp.287-295
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    • 2017
  • This study was conducted to observe egg and larvae morphological development of carp to obtain basic data for resource conservation and taxonomic research. Brood carp used in the research (total length 67.3-75.5 cm, average $71.0{\pm}3.45cm$) were bred in a circular rearing aquarium ($600{\times}300{\times}100cm$) using a running water system from January to July, 2015. Breeding water temperature was maintained at $23.0-25.0^{\circ}C$(average $24.0^{\circ}C$). Fertilized carp eggs were translucent and globular, and their size was 1.75-1.89 mm (average $1.82{\pm}0.06mm$). Blastoderms formed 10 min after fertilization and reached the two-cell stage 30 min after fertilization. Then, the embryo turned dark and exhibited melanophores, and blood started flowing from the heart across the egg yolk at 42 hrs and 50 min after fertilization. Hatching began 70 hrs and 26 min after fertilization larvae emerged through the egg membrane, starting from the head. The length of prelarvae immediately after hatching was 5.23-5.38 mm (average $5.31{\pm}0.11mm$) the mouth and anus were closed, and the pectoral fin was formed. Postlarvae at 18 days after hatching had a total length of 11.9-13.9 mm (average $12.9{\pm}1.40mm$), separate anal fin and back membranes, and fin ray. Juveniles fish at 35 days after hatching had a total length of 29.9-30.2 mm (average $30.1{\pm}0.13mm$), with the body covered with scales, and the same number of fin rays, color, and shape as their broodstork.

Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients

  • Xuan, Ying-Hua;Yu, Hak-Sun;Jeong, Hae-Jin;Seol, Sung-Yong;Chung, Dong-Il;Kong, Hyun-Hee
    • Parasites, Hosts and Diseases
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    • 제45권1호
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    • pp.1-9
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    • 2007
  • The endosymbionts of 4 strains of Acanthamoeba(KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of Acanthamoeba isolates. The endosymbionts of KA/E9, KA/E22, and KA/E23 were surrounded by electron-translucent areas. No lacunae-like structures were observed in the endosymbionts of KA/E21, the bacterial cell walls of which were studded with host ribosomes. Comparative analyses of the 16S rDNA sequences showed that the endosymbionts of KA/E9, KA/E22 and KA/E23 were closely related to Caedibacter caryophilus, whereas the KA/E21 endosymbiont was assigned to the Cytophaga-Flavobacterium-Bacteroides(CFB) phylum. In the 4 strains of Acanthamoeba, the hosts of the endosymbionts were identified as belonging to the Acanthamoeba castellanii complex, which corresponds to the T4 genotype. Acanthamoeba KA/E21 evidenced characteristics almost identical to those of KA/E6, with the exception of the existence of endosymbionts. The discovery of these endosymbionts from Acanthamoeba may prove essential to future studies focusing on interactions between the endosymbionts and the amoebic hosts.

양식산 터봇, Scophthalmus maximus에서의 irido-like virus 감염 (The infection of irido-like virus in cultured turbot)

  • 김위식;김기홍;김춘섭;김영진;정성주;정태성;키타무라 신이치;요시미즈 마모루;오명주
    • 한국어병학회지
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    • 제16권3호
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    • pp.153-159
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    • 2003
  • 본 연구는 병리조직학적 및 전자현미경적 방법을 통해 양식산 turbot에서 발생하는 Irido-like virus의 감염증을 밝히고자 한 것이다. 2003년 6월 전북 고창 소재 육상수조에서 양식중인 turbot 치어에서 50-70%의 대량폐사가 발생하였다. 병어는 사료섭취가 저하되고 힘없이 유영하다 폐사되었으며, 죽기 직전의 어류는 복부가 팽만되고 안구가 돌출되며 체색이 옅어지는 증상을 보였다. 해부학적 조사 결과, 아가미 빈혈과 간이 퇴색되어 있었으며, 특히 비장조직이 심하게 비대되어 있었다. 병어에 대한 조직학적 검사에서 비대 세포를 특징으로 하는 특이 병변이 주로 신장과 비장의 조혈조직에서 관찰되었으며, 아가미, 심장, 위, 장, 간, 췌장, 지방 조직에서도 관찰되었다. 그리고 전자현미경적 관찰 결과, 비대 세포의 세포질내에서 육각형의 지름이 136-159 nm인 Irido-like virus를 관찰할 수 있었다.

Age and Growth of Blackfin Flounder Glyptocephalus stelleri in the East Sea, Korea

  • Yang, Jae-Hyeong;Yoon, Sang-Chul;Lee, Sung-Il;Cha, Hyung-Kee;Kim, Jong-Bin;Choi, Young-Min;Park, Jeong-Ho
    • Fisheries and Aquatic Sciences
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    • 제15권2호
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    • pp.169-176
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    • 2012
  • Age and growth of Glyptocephalus stelleri in the East Sea of Korea were determined, from monthly samples of commercial catches, caught by the eastern sea Danish seine fishery in 2007. The annuli of G. stelleri are formed once a year, with the boundary between opaque and translucent zones forming in September. Relationships between total length (TL) and total weight (TW) were $TW=0.002TL^{3.392}$ ($r^2=0.970$) for females and $TW=0.002TL^{3.335}$ ($r^2=0.961$) for males. TLs at annuli formation in otoliths were back-calculated from the otolith-length relationship and were adjusted to von Bertalanffy growth curves to $L_t=39.71(1-{\exp}^{(-0.152(t+1.156))})$ for females and $L_t=32.16(1-{\exp}^{(-0.213(t+0.879))})$ for males. From the age of 3 years, females grew faster than males (P<0.05).

동해 포항 연안 문치가자미(Pseudopleuronectes yokohamae)의 연령과 성장 (The Age and Growth of Marbled Flounder Pseudopleuronectes yokohamae in the Coastal Waters of East Sea off Pohang)

  • 양재형;이해원;이재봉;차형기;김성태;윤병선
    • 한국수산과학회지
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    • 제50권3호
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    • pp.278-286
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    • 2017
  • The age and growth of marbled flounder Pseudopleuronectes yokohamae in coastal waters of the East Sea of Korea off Pohang were determined, by evaluating monthly samples of commercial catches, caught by gill nets and longlines from February 2013 to January 2014. New annuli from annually in P. yokohamae otoliths, and the boundary between the opaque and translucent zones forms from March to May. The relationships between total length (TL) and total weight (TW) were $TW=0.009TL^{3.128}$ ($R^2=0.969$) for females and $TW=0.008TL^{3.150}$ ($R^2=0.964$) for males. The TLs at the time of annulus formation, back-calculated from the otolith-length relationship by reference to the von Bertalanffy growth curves, were $L_t=52.63$ ($1-{\exp}^{(-0.231(t+0.033))}$) for females and $L_t=36.22$ ($1-{\exp}^{(-0.400(t+0.117))}$) for males. Commencing at the age of 3 years, females grew faster than males (P<0.05).

레조르시놀 비스-에틸헥사노에이트를 함유한 나노에멀젼의 유화제 종류에 따른 형성 (Formation of Nano-emulsions with Resorcinol bis-ethylhexanoate upon Type of Emulsifiers)

  • 조완구
    • 대한화장품학회지
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    • 제42권1호
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    • pp.29-35
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    • 2016
  • 본 연구에서는 미백제인 RS White를 함유한 O/W 나노에멀젼의 형성 가능성을 유화제 종류별로 실험하였다. 친수성계면활성제로 Tween 80, 60, HCO 60 및 40과 친유성 계면활성제로 Span 80을 사용한 시스템에서는 적정한 농도에서 나노에멀젼이 형성되었으나 Myrj 52, Montanov L 및 Tegocare 450과 Span 80을 사용한 시스템에서는 형성되지 않았다. 형성된 나노에멀젼은 입자의 직경이 100 nm 미만의 반투명 외관을 보였으며 시간 경과에 따른 안정성 평가에서도 안정성을 유지하였다. 이들의 경피흡수 정도를 마크로에멀젼과 비교한 결과 24 h 동안 피부 투과량은 나노에멀젼은 $70.84{\mu}g/cm^2$, O/W 마크로에멀젼은 $28.97{\mu}g/cm^2$으로 나타났다. 따라서, 본 연구는 RS White 미백제를 함유한 안정한 나노에멀젼이 기능성 소재의 효율적인 피부 전달체로서 응용 가능성이 있음을 시사한다.

방사선치료에서 3D 프린터를 이용한 기능적 조직보상체의 제작 (Manufacturing a Functional Bolus Using a 3D printer in Radiation Therapy)

  • 이이성;김정구
    • 대한방사선기술학회지:방사선기술과학
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    • 제43권1호
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    • pp.9-14
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    • 2020
  • Commercial plate bolus is generally used for treatment of surface tumor and required surface dose. We fabricated 3D-printed bolus by using 3D printing technology and usability of 3D-printed bolus was evaluated. RT-structure of contoured plate bolus in the TPS was exported to DICOM files and converted to STL file by using converting program. The 3D-printed bolus was manufactured with rubber-like translucent materials using a 3D printer. The dose distribution calculated in the TPS and compared the characteristics of the plate bolus and the 3D printed bolus. The absolute dose was measured inserting an ion chamber to the depth of 5 cm and 10 cm from the surface of the blue water phantom. HU and ED were measured to compare the material characteristics. 100% dose was distributed at Dmax of 1.5 cm below the surface when was applied without bolus. When the plate bolus and 3D-plate bolus were applied, dose distributed at 0.9 cm and 0.8 cm below the surface of the bolus. After the comparative analysis of the radiation dose at the reference depth, differences in radiation dose of 0.1 ~ 0.3% were found, but there was no difference dose. The usability of the 3D-printed bolus was thus confirmed and it is considered that the 3D-printed bolus can be applied in radiation therapy.

범가자미, Verasper variegatus의 연령과 성장 (Age and Growth of Spotted Halibut, Verasper variegatus)

  • 전복순;박병하;전임기;강용주
    • 한국어류학회지
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    • 제8권1호
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    • pp.56-63
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    • 1996
  • 1992년 12월부터 1993년 12월까지 매월 1회씩 남해안에서 어획된 범가자미를 대상으로 연령과 성장을 조사하였다. 연령사정은 이식으로 하였으며, 윤문은 투명대에서 불투명대로 이행하는 경계로 하였다. 윤문은 2월부터 3월 사이에 연 1회 형성되었다. 이석경(R)에 대한 전장(TL)의 관계를 보면 암컷은 TL=18.0329R-11.6879, 수컷은 TL=15.1458R-5.6902였다. 전장(TL)에 대한 체중(BW)의 관계는 암컷은 $BW=0.0065TL^{3.2042}$, 수컷은 $BW=0.0146TL^{2.9620}$이었다. 연령(t)에 대한 체장(Lt)의 Von Bertalanffy 성장식은 암컷은$L_t=71.0783(1-e^{-0.3036(t-0.1173)})$, 수컷은 $L_t=57.4414(1-e^{-0.2931(t+0.1590)})$이었다. 채집된 표본 중 암컷은 6세, 수컷은 4세까지 있었지만 대부분 3세 이하였다.

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현대 복식에 표현된 투명디자인의 조형성 (Plasticity of Transparent Design Expressed in Contemporary Fashion)

  • 정연자
    • 한국의상디자인학회지
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    • 제3권2호
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    • pp.51-62
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    • 2001
  • This study was intended to investigate the contemporary meaning of transparent design expressed in contemporary fashion by inquiring into its plasticity expressed in contemporary design and fashion and analyzing the association between them. It used the comparative anesthetic method widely used by H. W lfflin and other scholars in eliciting the differences and similarities in styles. It attempted to investigate the plasticity of transparent design expressed in contemporary design by dividing it into the epidermal, spatial and ideological effects. Contemporary design expressed the epidermal effects such as lightness(nimbleness), smoothness, contrast and stress It expressed the spatial effects such as spatial flexibility through the interpenetration of interior and exterior spaces, expansion of the space and the like. And it expressed ideological effects such as openness, pureness, playfulness and the like. In terms of lightness, contemporary design expressed actual or visual lightness by using transparent materials. In terms of the interpenetration of interior and exterior spaces, it expressed the interpenetration of the interior space into the exterior spaces and vice versa. In terms of spatial expansion, contemporary design expressed the infinite spatial connection by drawing the exterior space into the interior space. Contrast and stress were formed by imposing visual unity on contemporary fashion by using opaque and transparent materials. In terms of openness, contemporary design expressed contemporary people's candor and dependability by making the inside of the building or object look outward. To express pureness, contemporary design used the clear and transparent image by using the glass or translucent materials taking on the pure property. To express playfulness, contemporary made use of the vagueness of the spatial boundary, the locational switching of inner and outer spaces, and interesting external forms occurring when light pass through opaque and transparent materials.

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Unity3D 게임 엔진을 이용한 의료 데이터 가시화 (Medical data visualization using Unity3D game engine)

  • 하태준;계희원
    • 한국컴퓨터그래픽스학회논문지
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    • 제23권3호
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    • pp.87-94
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    • 2017
  • 상용의 게임 엔진의 기능이 향상되면서 과학적 가시화 프로그램에 게임 엔진을 적용하는 사례가 증가하고 있다. 본 연구는 Unity3D 게임 엔진을 기반으로, 의료 볼륨 데이터를 가시화하는 가상현실 응용프로그램의 제작 사례를 설명한다. 게임 엔진을 사용하는 경우, 반투명한 물체의 깊이 정렬이나 가상현실 하드웨어 지원과 같이, 응용 프로그램이 필요한 다양한 기능이 기본적으로 제공되는 장점이 있다. 한편, 게임 엔진의 특성에 적합하도록 응용 프로그램의 구조를 수정하여 적용해야 하는 제약이 있다. 본 연구는 게임 엔진의 구조를 이용하여 의료 볼륨 데이터의 가시화를 수행하는 방법에 대해 설명한다. 그 결과, 표면 데이터와 의료 볼륨 데이터 조각들이 함께 구성된 가상현실 장면을 생성할 수 있었으며, 게임 엔진의 의료 시뮬레이션 제작 도구로서의 가능성을 확인하였다.